BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	pSIJ663_pHT315_araE_xy	3,783	G→T	5.2%	intergenic (+68/‑88)	eryR → / → ampR	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	pSIJ663_pHT315_araE_xy	3,783	0	G	T	5.2%	5830.1 / 41.9	1796	intergenic (+68/‑88)	eryR/ampR	–/–
Reads supporting (aligned to +/- strand): ref base G (543/1100); new base T (110/31); total (660/1136)
Fisher's exact test for biased strand distribution p-value = 1.54e-25
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1838 total aligned reads displayed.

AAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGTATCGGGGTACATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTG  >  pSIJ663_pHT315_araE_xy/3646‑3913

aaGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAg                                                                                                                                    <  7:293656/138‑1 (MQ=255)

 aGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGat                                                                                                                                  <  2:287388/139‑1 (MQ=255)

 aGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGat                                                                                                                                  <  2:228783/139‑1 (MQ=255)

 aGTACCATTACATATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGat                                                                                                                                  <  7:233253/139‑1 (MQ=255)

           cTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGa                                                                                                                        <  3:87380/139‑1 (MQ=255)

           cTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGa                                                                                                                        <  2:202949/139‑1 (MQ=255)

              aTGAGCAATGATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGATTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAATTTATTAGATATACTACTGAc                                                                                                                       <  4:191990/137‑1 (MQ=255)

                gAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCGTTTTTATATTTTGAAAGTTTAACGTTTCTTAATGGTATTTAGATAAATTTTTATATATAATACTGAAAGCt                                                                                                                   >  5:131553/1‑139 (MQ=255)

                 aGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTTAATTTTGTAAGTTACACGTTTATAAAGGGAATGGAGATAAATTATTAGATATAATACTTAAAGcgt                                                                                                                  >  5:273927/1‑137 (MQ=255)

                    aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                                                               <  5:40745/139‑1 (MQ=255)

                    aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                                                               <  3:249771/139‑1 (MQ=255)

                     aGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaa                                                                                                              <  8:169122/139‑1 (MQ=255)

                      gTATTGTCTATTTTTAATAGTTATCTTTTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTATATGGTATGGAGATAAATTATTTGATATACTACTGACAGCTTCCaag                                                                                                             <  4:14263/139‑1 (MQ=255)

                      gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCTCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaag                                                                                                             <  4:10513/139‑1 (MQ=255)

                      gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaag                                                                                                             <  5:58829/139‑1 (MQ=255)

                      gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaag                                                                                                             <  4:273981/139‑1 (MQ=255)

                      gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaag                                                                                                             <  1:273601/139‑1 (MQ=255)

                      gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTAGGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaag                                                                                                             <  7:9399/139‑1 (MQ=255)

                        aTTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaaga                                                                                                            <  7:217428/138‑1 (MQ=255)

                           gTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGc                                                                                                         <  7:27398/138‑1 (MQ=255)

                                         gTTATATATTATTTAACGGTAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAg                                                                                          <  8:207064/139‑1 (MQ=255)

                                                   attTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACgg                                                                                 <  3:5157/138‑1 (MQ=255)

                                                   attTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACgg                                                                                 <  3:201419/138‑1 (MQ=255)

                                                   attTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACgg                                                                                 <  5:291866/138‑1 (MQ=255)

                                                           ggaggaAATAATTCTATGAGTCGCGTTTTTTAATTTGGAAAGTTTGACGGTTGTAAATGGGTAGGAGATAAATTATTTGTTATTATACCGACCACGTGCAAGAAGCGAAAAAGGTGCCTAGCGCCGACGGGGTAtgtgg                                                                        >  4:40222/1‑138 (MQ=255)

                                                                 aataatTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGATATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtaca                                                                  <  1:73144‑M1/139‑8 (MQ=255)

                                                                 aataatTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtaca                                                                  <  1:9515‑M1/139‑8 (MQ=255)

                                                                   taatTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacatt                                                                <  4:241918‑M1/139‑10 (MQ=255)

                                                                        cTATGAGTCGCTTTTTTAAATTTGTAAAGTTACACGTTACTAAAGGGAATGTAGATAAATTATTAGATAAACTACTGACCGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaa                                                            <  4:220354‑M1/138‑14 (MQ=255)

                                                                        cTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaa                                                            <  3:229074‑M1/138‑14 (MQ=255)

                                                                           tGAGTCTCTTTTTTAAATTTGGAAAGTTCAACTTTACTTAAGGGAATGGATATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                        <  7:209258‑M1/139‑18 (MQ=255)

                                                                           tGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGTAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                        <  3:216362‑M1/139‑18 (MQ=255)

                                                                           tGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTATATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                        <  5:105599‑M1/139‑18 (MQ=255)

                                                                           tGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                        <  1:1646‑M1/139‑18 (MQ=255)

                                                                           tGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                        <  2:247703‑M1/139‑18 (MQ=255)

                                                                            tagTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                        <  1:145537‑M1/137‑18 (MQ=255)

                                                                            gAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                        <  5:216343‑M1/138‑18 (MQ=255)

                                                                            gAGTCGCTTTTTTAAATTTCGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                        <  8:61804‑M1/138‑18 (MQ=255)

                                                                            gAGTAGCTTTTTTAAATTTTGAAAGTTACACGTTACTAAAGGGATTGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                        <  6:77851‑M1/138‑18 (MQ=255)

                                                                             aGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa                                                      <  1:231148‑M1/139‑20 (MQ=255)

                                                                             aGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa                                                      <  6:101413‑M1/139‑20 (MQ=255)

                                                                             aGTCGCTTTTATAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa                                                      <  1:121238‑M1/139‑20 (MQ=255)

                                                                                 gCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagag                                                   <  8:274528‑M1/138‑23 (MQ=255)

                                                                                    tttttAAATTTGGAAAGTTACACGTTACTAAAGGTAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                                                <  7:150347‑M1/138‑26 (MQ=255)

                                                                                     ttttAAATTTGGAAAGTTACACGTTACTAAAGGGAATTGAGATAAATTATTATATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                                                <  1:100052‑M1/137‑26 (MQ=255)

                                                                                     ttttAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                                                <  1:203897‑M1/137‑26 (MQ=255)

                                                                                      tttAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatga                                              <  3:123490‑M1/138‑28 (MQ=255)

                                                                                         aaaTTTGGAAAGTTACACTTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGATAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagta                                           <  7:48540‑M1/138‑31 (MQ=255)

                                                                                         aaaTTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagta                                           <  2:113008‑M1/138‑31 (MQ=255)

                                                                                         aaaTTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACAACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagta                                           <  1:284843‑M1/138‑31 (MQ=255)

                                                                                          taTTTTGAAATTTACTAGTTAATATATGGATTGGATATAAATTATTATATATAGTACTGTCAGCTTCCAAGAAGATAAAGAGGTACCTAGCGCCTACGGGGAATTT‑‑‑‑‑GGGGTACATTGATAAAGGAAGAGTATGAGTAtt                                    <  1:251298/138‑1 (MQ=255)

                                                                                              tGGAAAGTTACACGTTACTAAAGGGAATGTATATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGATAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaa                                      <  3:278379‑M1/138‑36 (MQ=255)

                                                                                               ggAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaaca                                    >  4:83397‑M1/1‑102 (MQ=255)

                                                                                               ggAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaaca                                    >  8:153901‑M1/1‑102 (MQ=255)

                                                                                                        cacGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgt                           <  5:147850‑M1/139‑47 (MQ=255)

                                                                                                        cacGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgt                           <  7:61585‑M1/139‑47 (MQ=255)

                                                                                                        cacGTTACTAAAGGGAATGCATATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgt                           <  7:259681‑M1/139‑47 (MQ=255)

                                                                                                           gTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgc                        <  5:199742‑M1/139‑50 (MQ=255)

                                                                                                             tACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                        >  6:2384‑M1/1‑88 (MQ=255)

                                                                                                             tACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGggg                                                                      >  5:176348‑M1/1‑88 (MQ=255)

                                                                                                               cTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgccct                     >  4:123746‑M1/1‑86 (MQ=255)

                                                                                                               cTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgccct                     >  7:108862‑M1/1‑86 (MQ=255)

                                                                                                               cTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagga                                                       <  3:31623‑M1/104‑19 (MQ=255)

                                                                                                                  aaGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttatt                 >  8:115976‑M1/1‑83 (MQ=255)

                                                                                                                     ggAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGtggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattccc              >  8:227295‑M1/1‑80 (MQ=255)

                                                                                                                     ggAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattccc              >  6:283243‑M1/1‑80 (MQ=255)

                                                                                                                     ggAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtatt                                         >  2:75438‑M1/1‑80 (MQ=255)

                                                                                                                     ggAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGATAAAGAGGTCCCTAGCGCCTACGCGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtctcccttattccc              >  2:148699‑M1/1‑80 (MQ=255)

                                                                                                                       aaTGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattccctt            >  6:3859‑M1/1‑78 (MQ=255)

                                                                                                                       aaTGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattccctt            >  4:61428‑M1/1‑78 (MQ=255)

                                                                                                                       aaTGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattccctt            >  7:188301‑M1/1‑78 (MQ=255)

                                                                                                                       aaTGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattccctt            >  2:5109‑M1/1‑78 (MQ=255)

                                                                                                                       aaTGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattccctt            >  6:53907‑M1/1‑78 (MQ=255)

                                                                                                                       aaTGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattccctt            >  6:264549‑M1/1‑78 (MQ=255)

                                                                                                                       aaTGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattccctt            >  6:12033‑M1/1‑78 (MQ=255)

                                                                                                                       aaTGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgccc                      >  6:162964‑M1/1‑78 (MQ=255)

                                                                                                                       aaTGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagta                                           <  7:80760‑M1/108‑31 (MQ=255)

                                                                                                                       aaTGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                                                <  6:137718‑M1/103‑26 (MQ=255)

                                                                                                                       aaTGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                                                <  8:43023‑M1/103‑26 (MQ=255)

                                                                                                                       aaTGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa                                                      <  6:211491‑M1/97‑20 (MQ=255)

                                                                                                                       aaTGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                        <  5:254990‑M1/95‑18 (MQ=255)

                                                                                                                       aaTGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                        <  4:16718‑M1/95‑18 (MQ=255)

                                                                                                                       aaTGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATATGgggtacattgaaaaaggaagagtatgagtattcaacatttcagtctcgccattattacctt            >  8:185766‑M1/1‑78 (MQ=255)

                                                                                                                        aTGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttt           >  8:136066‑M1/1‑77 (MQ=255)

                                                                                                                         tGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattccctttt          >  8:82237‑M1/1‑76 (MQ=255)

                                                                                                                         tGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatga                                              >  7:168935‑M1/1‑76 (MQ=255)

                                                                                                                         tGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa                                                      >  1:181879‑M1/1‑76 (MQ=255)

                                                                                                                         tGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggta                                                                    <  6:185995‑M1/81‑6 (MQ=255)

                                                                                                                          ggAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagta                                           <  7:124155‑M1/105‑31 (MQ=255)

                                                                                                                            agaTAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                                                <  5:238264‑M1/98‑26 (MQ=255)

                                                                                                                             tatAAATTATAAGATATACTACTGACAGCTTCCAAGAAGATAAAGAGGTCCCTAGCGCCTACGGTGAATTTGgggtacattgaaaaaggaagagtatgagtattcaaaatttccgtgtcgcccttattcccttttttg       <  8:291281‑M1/137‑67 (MQ=255)

                                                                                                                              aTAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcg     >  2:17575‑M1/1‑71 (MQ=255)

                                                                                                                              aTAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa                                                      >  3:69912‑M1/1‑71 (MQ=255)

                                                                                                                               tAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcgg    >  8:251207‑M1/1‑70 (MQ=255)

                                                                                                                               tAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcgg    >  5:8204‑M1/1‑70 (MQ=255)

                                                                                                                                 aaTTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggca  >  6:251055‑M1/1‑68 (MQ=255)

                                                                                                                                 aaTTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggca  >  3:154406‑M1/1‑68 (MQ=255)

                                                                                                                                      ttaGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                                                <  1:43104‑M1/88‑26 (MQ=255)

                                                                                                                                      ttaGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                                                <  3:10502‑M1/88‑26 (MQ=255)

                                                                                                                                      ttaGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtaca                                                                  <  3:18097‑M1/70‑8 (MQ=255)

AAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGTATCGGGGTACATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTG  >  pSIJ663_pHT315_araE_xy/3646‑3913

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`