BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	pSIJ663_pHT315_araE_xy	4,648	T→A	10.0%	Y260N (TAC→AAC) ‡	ampR →

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	pSIJ663_pHT315_araE_xy	4,648	0	T	A	10.0%	3958.7 / 93.4	1435	Y260N (TAC→AAC) ‡	ampR
Reads supporting (aligned to +/- strand): ref base T (957/314); new base A (2/155); total (960/475)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1452 total aligned reads displayed.

TTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTT  >  pSIJ663_pHT315_araE_xy/4511‑4785

   cAGGCCCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGGGGGTCTCGCGGTAGCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTacac                                                                                                                                       >  3:66840/1‑139 (MQ=255)

   cAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTacac                                                                                                                                       >  7:230844/1‑139 (MQ=255)

   cAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGAAGCACTGGGGCCAGATGGTAAGACCTCCCGTATCGTAGTTATCTacac                                                                                                                                       >  3:161280/1‑139 (MQ=255)

        ccACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTATATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTAGCATTGCAGCACAGGGGCCAGATGGTAAGCCCTCCCGTATCGAAGTTATCTACAGGACgg                                                                                                                                  >  1:123164/1‑139 (MQ=255)

              cTGCGCTCGGCCCATCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTAGCGTAGTTATCTACACGACGGGGAGt                                                                                                                             >  5:79129/1‑138 (MQ=255)

                 cgggcGGCCCTGCCGGGGGGCTGGTTTATTGATGATAATTCTGGTGCGGGTGCGCGGGGGTCGCGCGGTATCATTGCCGCACTGGGCCCTGTTGGTACGCCCTCCCGTATCGTAGTTATCTCCACGACGGGGAGTCAgg                                                                                                                         <  2:80661/135‑1 (MQ=255)

                 cgcTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAgg                                                                                                                         >  3:90276/1‑139 (MQ=255)

                 cgcTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAgg                                                                                                                         >  4:122689/1‑139 (MQ=255)

                     cGGCCCTTCCGGCTGGCTGGTTTATTGATTATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGCTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCaa                                                                                                                      >  1:95801/1‑138 (MQ=255)

                      ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTACGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAAc                                                                                                                     >  3:211509/1‑138 (MQ=255)

                      ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTACGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAAc                                                                                                                     >  8:213756/1‑138 (MQ=255)

                      ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAAc                                                                                                                     >  8:261511/1‑138 (MQ=255)

                      ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAAc                                                                                                                     >  3:133243/1‑138 (MQ=255)

                      ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAAc                                                                                                                     >  7:54361/1‑138 (MQ=255)

                      ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAAc                                                                                                                     >  7:273239/1‑138 (MQ=255)

                      ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAAc                                                                                                                     >  4:139853/1‑138 (MQ=255)

                      ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAAc                                                                                                                     >  2:206821/1‑138 (MQ=255)

                      ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAAc                                                                                                                     >  4:33115/1‑138 (MQ=255)

                      ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAAc                                                                                                                     >  6:257046/1‑138 (MQ=255)

                           ttCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGa                                                                                                               >  7:217934/1‑139 (MQ=255)

                              cGGGTGGCTGTTTTATTGCTTATAAATCTGGCGCCGGTGCGGGTGGGGCGCGCGGAATCATTGCCGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGa                                                                                                            <  3:181890/139‑1 (MQ=255)

                              cGGCTGTCGGGTTTATGGATTTTATCTCTGGCGCGGGTGCGCGGGGGTCGCGGGGAATCATTGCAGCACTGGGGCAATATGTTACGCCATCCCTTCTGTTAGTTATAAACACGACGGGGAGTCAGGCAACTATGGATGa                                                                                                            <  6:17148/139‑1 (MQ=255)

                                  tggctggTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACg                                                                                                         >  6:93503/1‑138 (MQ=255)

                                  tggctggTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACg                                                                                                         >  7:37466/1‑138 (MQ=255)

                                  tggctggTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGAAGTTATCTACACGACCGGGAGTCAGGCAACTATGGATGAACg                                                                                                         >  8:113120/1‑138 (MQ=255)

                                           tATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGa                                                                                                  >  6:209584/1‑136 (MQ=255)

                                                  gATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCt                                                                                         >  3:116426/1‑138 (MQ=255)

                                                  gATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCt                                                                                         >  1:82017/1‑138 (MQ=255)

                                                   aTAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCt                                                                                         >  1:245408/1‑137 (MQ=255)

                                                      aaTCGGGCGCGGGGGAGGGGGGGGATCGGGGTAACTTTCCAGCACTGGGCCCAGTTGGTAAGCCCCCCCGTATGGTAGTTATCAACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGAt                                                                                    <  5:141285/139‑1 (MQ=255)

                                                            gAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTATTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGc                                                                              >  2:255246/1‑139 (MQ=255)

                                                                cGGTGTGCGGGGGTCTGGCGTTATAATGGCTGCACGGGGGCACTATGGTACGCCCTCCCGTATCGTAGTTATATACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCa                                                                          <  2:272382/139‑1 (MQ=255)

                                                                 ggTGTGCGTGGGGCGCGCGTTATATTTGCAGCACTGGGGCCATATGTTAAGCCCTCCCTTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCAc                                                                         <  8:173309/139‑1 (MQ=255)

                                                                  gTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGCCGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACt                                                                        >  8:34826/1‑139 (MQ=255)

                                                                  gTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACt                                                                        >  1:175221/1‑139 (MQ=255)

                                                                  gTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACt                                                                        >  6:268124/1‑139 (MQ=255)

                                                                        gggggTCTCGCGGTATTTTGCCAGCCGGGGGGCATTATGGTACGCCCTCCCTTTTGTTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTaa                                                                  <  7:128358/137‑1 (MQ=255)

                                                                        cgtggTTTCGCGTTATCATTGCACCTCTGGGGCATGTTTGTAAGCCCTCCCTTATGGTTTTTATCTCCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTaa                                                                  <  6:296998/136‑1 (MQ=255)

                                                                         ggggTCGCGGGTTAGCATTGCAGCAGTGGGGCCAGTTGGAACGCCCTCCCGTAGCGTAGTTATCACCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAg                                                                 <  5:252795/138‑1 (MQ=255)

                                                                           ggTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCa                                                               >  1:52318/1‑139 (MQ=255)

                                                                           ggTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCa                                                               >  1:187531/1‑139 (MQ=255)

                                                                                gggggTATCATTGCAGCCGGGGGGCCAGTTGGAACGCCCTCCCTTCTCGTATTTATACACACGACGGGGAGTCAGGCAACAATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGt                                                          <  4:251827/136‑1 (MQ=255)

                                                                                ggctttATCATTCCAGCAGGGGGGCCTTATGTTAAGCCCGCCCGTATCTTAGTTACAAACACGACGGGGAGGCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGt                                                          <  5:203603/134‑1 (MQ=255)

                                                                                       tCATTGCAGCACTTGGGCCAGATGGTAAGCCCTCACGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGAAGAAAGAAATAGACAGATCGCTGAGATAGGTCCCTCACGGATAAAGCATTGGTAACTGt                                                    >  4:56911/1‑138 (MQ=255)

                                                                                           tGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTcctgt                                               >  3:226448/1‑135 (MQ=255)

                                                                                            tccgctcTGGGGCCTGATGGTACGCCCTCCGGTATATTTCTAACCTACACGTCGGGGAGTCAGGCATCTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGAc                                               <  7:7345/132‑1 (MQ=255)

                                                                                            gccccaCTGGGGCCAGTTGGTAAGCCCTCCCGTATCGTAGTTAGCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGAcc                                              <  4:107167/135‑1 (MQ=255)

                                                                                               gcaCTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAg                                           >  8:83271/1‑139 (MQ=255)

                                                                                               gcaCTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAg                                           >  5:63147/1‑139 (MQ=255)

                                                                                               gcaCTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAg                                           >  8:274669/1‑139 (MQ=255)

                                                                                                 acgggggCCCGATGGTAAGCCCTCCCGTATCGCAGTAATCAACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGtt                                         <  8:67679/136‑1 (MQ=255)

                                                                                                   tGGGGCCAGATGGTAACCCCTCCCTTATCGTAGTAAACACCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                       <  7:261511/139‑1 (MQ=255)

                                                                                                   tGGGGCAAGTTGGAAAGCCGGCCCGTATCGTAGTAATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                       <  8:190257/139‑1 (MQ=255)

                                                                                                   tGGGGACATATGGTACCCCCACCGGTAGCTTAGTAATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                       <  1:14945/139‑1 (MQ=255)

                                                                                                   gggggCCTGATGGTAAGCCCTCCGGTTTGGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                       <  4:225286/138‑1 (MQ=255)

                                                                                                   ggggcCCAGATTGTAACCCCCCCCTTATCTTAGTAATAACCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                       <  3:43407/138‑1 (MQ=255)

                                                                                                    ggggCCTGTTGGTACGCCCCCCCGTATCGTATTTATCAACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTAc                                      <  1:204021/139‑1 (MQ=255)

                                                                                                     gggCCTGATGGAAACCCCTCCCGTAGCGAAGTAAACTCCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                       <  3:284899/137‑1 (MQ=255)

                                                                                                     gggCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCCACTATGGATGAACGAAATAGACAGATCGCGGAGATAGGTGCCTCACTGAGTAAGCATTGGTAACTGTCAGACCTCGTTTagt                                     >  8:139620/1‑137 (MQ=255)

                                                                                                     gggCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGCTCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTaat                                     >  4:191297/1‑137 (MQ=255)

                                                                                                     gggCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGt                                                    >  1:22725/1‑124 (MQ=255)

                                                                                                     gggCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                       <  2:160494/137‑1 (MQ=255)

                                                                                                     gggCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTAc                                      >  2:227372/1‑138 (MQ=255)

                                                                                                     gggCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACt                                     >  1:290519/1‑139 (MQ=255)

                                                                                                     gggCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCAACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                       <  5:105508/137‑1 (MQ=255)

                                                                                                     gggCCAGATGGTAAGCCCTCCCGTATCGAAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATCAACGAAATAGACAGATCCCTGAGATAGGTGCCTCACTGCTTAAGCATTGGTAACTGTCATCCCAAGTTTAc                                      >  2:125663/1‑138 (MQ=255)

                                                                                                     ggcccAGAGGGAACGCCCACCCGATTGGTTGTTATCTCCACGACGGGGAGTCAGGCAACTAGGCATGAACGAAATAGACCGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACt                                     <  6:72202/136‑1 (MQ=255)

                                                                                                          aGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCata                                 >  7:181226/1‑138 (MQ=255)

                                                                                                          aGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCata                                 >  8:48168/1‑138 (MQ=255)

                                                                                                           cttGGTAAGCCCCCCCGTATCGTAGTTATCACCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCatata                               <  7:159274/137‑1 (MQ=255)

                                                                                                            aTGTTAACCCCTCCGGTATGGTATTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCatat                                <  6:224884/137‑1 (MQ=255)

                                                                                                            aTGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGAc                                               <  3:103389/122‑1 (MQ=255)

                                                                                                            aTGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCatata                               >  2:39126/1‑138 (MQ=255)

                                                                                                                tAAGCCCTCCCGTTTCGTAGTTATATACCCGACGGGGAGTCAGGCAACTATGGATGCACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACtt                          >  6:54887/1‑139 (MQ=255)

                                                                                                                 aaGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACtt                          >  4:207669/1‑138 (MQ=255)

                                                                                                                  aGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTgag                                                                                      >  6:122559/1‑77 (MQ=255)

                                                                                                                     ccTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAgat                     <  8:232228/139‑1 (MQ=255)

                                                                                                                      cTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                       >  2:61728/1‑120 (MQ=255)

                                                                                                                      cTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAgatt                    >  1:102191/1‑139 (MQ=255)

                                                                                                                       tCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAgatt                    >  5:126711/1‑138 (MQ=255)

                                                                                                                       tCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAgatct                   >  5:224884/1‑137 (MQ=255)

                                                                                                                        cccGTATCGTTGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCaa                                            >  3:248295/1‑113 (MQ=255)

                                                                                                                        cccGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                       >  4:271105/1‑118 (MQ=255)

                                                                                                                        cccGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                       >  5:97212/1‑118 (MQ=255)

                                                                                                                        cccGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                       <  5:278282/118‑1 (MQ=255)

                                                                                                                        cccGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAgattga                  >  5:175038/1‑139 (MQ=255)

                                                                                                                        cccGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGAAAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAgattga                  >  7:290339/1‑139 (MQ=255)

                                                                                                                              tCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGAc                                               <  2:176959/104‑1 (MQ=255)

                                                                                                                                 tAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTc                                    <  1:27235/112‑1 (MQ=255)

                                                                                                                                 tAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCatat                                <  3:143698/116‑1 (MQ=255)

                                                                                                                                 tAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACt         >  4:149783/1‑139 (MQ=255)

                                                                                                                                 tAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACt         >  6:13379/1‑139 (MQ=255)

                                                                                                                                 tAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACAAAGTTTACTCATATATACTTTAGATTGATTTAAAAcc         >  3:265371/1‑138 (MQ=255)

                                                                                                                                   gTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACtt        >  5:40689/1‑138 (MQ=255)

                                                                                                                                   gTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACtt        >  2:185583/1‑138 (MQ=255)

                                                                                                                                      aTCTCCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                       <  4:169488/104‑1 (MQ=255)

                                                                                                                                      aTCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGt                                                          >  7:88870/1‑85 (MQ=255)

                                                                                                                                      aTCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGtt                                         >  7:195958/1‑102 (MQ=255)

                                                                                                                                      aTCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCatat                                >  1:71056/1‑111 (MQ=255)

                                                                                                                                         tACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCAtttt  >  4:81578/1‑138 (MQ=255)

TTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTT  >  pSIJ663_pHT315_araE_xy/4511‑4785

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`