BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	pSIJ663_pHT315_araE_xy	4,649	A→C	7.1%	Y260S (TAC→TCC) ‡	ampR →

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	pSIJ663_pHT315_araE_xy	4,649	0	A	C	7.1%	3588.8 / 59.0	1403	Y260S (TAC→TCC) ‡	ampR
Reads supporting (aligned to +/- strand): ref base A (916/357); new base C (4/122); total (923/480)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1457 total aligned reads displayed.

TTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAA‑GCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTA  >  pSIJ663_pHT315_araE_xy/4511‑4787

   cAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAA‑GCCCTCCCGTATCGTAGTTATCTacac                                                                                                                                         >  6:32950/1‑139 (MQ=255)

   cAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAA‑GCCCTCCCGTATCGTAGTTATCTacac                                                                                                                                         >  7:230844/1‑139 (MQ=255)

   cAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGAAGCACTGGGGCCAGATGGTAA‑GACCTCCCGTATCGTAGTTATCTacac                                                                                                                                         >  3:161280/1‑139 (MQ=255)

        ccACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTATATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTAGCATTGCAGCACAGGGGCCAGATGGTAA‑GCCCTCCCGTATCGAAGTTATCTACAGGACgg                                                                                                                                    >  1:123164/1‑139 (MQ=255)

                 cgggcGGCCCTGCCGGGGGGCTGGTTTATTGATGATAATTCTGGTGCGGGTGCGCGGGGGTCGCGCGGTATCATTGCCGCACTGGGCCCTGTTGGTAC‑GCCCTCCCGTATCGTAGTTATCTCCACGACGGGGAGTCAgg                                                                                                                           <  2:80661/135‑1 (MQ=255)

                 cgcTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAA‑GCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAgg                                                                                                                           >  4:122689/1‑139 (MQ=255)

                      ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAC‑GCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAAc                                                                                                                       >  8:213756/1‑138 (MQ=255)

                      ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAC‑GCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAAc                                                                                                                       >  3:211509/1‑138 (MQ=255)

                      ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAA‑GCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAAc                                                                                                                       >  8:261511/1‑138 (MQ=255)

                      ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAA‑GCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAAc                                                                                                                       >  4:33115/1‑138 (MQ=255)

                      ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAA‑GCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAAc                                                                                                                       >  4:139853/1‑138 (MQ=255)

                      ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAA‑GCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAAc                                                                                                                       >  3:4447/1‑138 (MQ=255)

                      ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAA‑GCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAAc                                                                                                                       >  3:133243/1‑138 (MQ=255)

                      ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAA‑GCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAAc                                                                                                                       >  7:273239/1‑138 (MQ=255)

                      ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAA‑GCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAAc                                                                                                                       >  7:54361/1‑138 (MQ=255)

                      ggCCCTTCCGCCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGAGGGTCTCGCGGTATCATTGCAGCACTGGGGCCCGATGGTAA‑GCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCaca                                                                                                                       >  8:115758/1‑136 (MQ=255)

                           ttCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAA‑GCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGa                                                                                                                 >  5:281201/1‑139 (MQ=255)

                           ttCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAA‑GCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGa                                                                                                                 >  7:217934/1‑139 (MQ=255)

                           ttCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAA‑GCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGa                                                                                                                 >  5:63232/1‑139 (MQ=255)

                              ggggtgggtgtTTTATGGGTGATAATCGTGGAGCCGGTTAGGGTGGGGCGGGCGGTATCTTTCCAGCCCGGGGGCCAGATGGTAA‑GCCCTCCCGTAGCGTAGTTATCTCCACGACGGGGAGTCAGGCAACTATGGATGa                                                                                                              <  6:257563/135‑1 (MQ=255)

                                  tggctggTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAA‑GCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACg                                                                                                           >  7:37466/1‑138 (MQ=255)

                                        gTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAA‑GCCCTCCCGTATCTTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGATATAGa                                                                                                    >  2:160912/1‑139 (MQ=255)

                                           tATTGCTTATAACTCTGTAGAGGGGGAGGGGGGTTCGGGCGTTTTCATTGCAGCAGGGGGGCCTTTTGGTAA‑GCGCACCCGTTTCGTTTTTATATACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACa                                                                                                  <  6:96404/138‑1 (MQ=255)

                                            ttggCTGATAAATCGGGGGCGGGTGCGGGTGGGGGTCGGGTTTTCTTTCCACCGCTCGGGCCATATGGATA‑CCCCTCCGTTTTCGTTTTTATATCCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAg                                                                                                 <  2:62041/135‑1 (MQ=255)

                                                  taTAAATGTGGCGCGGGGGAGGGGGGGGCGCGCGGTATCATTGCCGCACGGGGGCCATTGGGTAC‑GCCCTCCCTTATCGTAGTTATCTCCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCt                                                                                           <  6:134951/137‑1 (MQ=255)

                                                  gATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAA‑GCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCt                                                                                           >  1:82017/1‑138 (MQ=255)

                                                  gATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAA‑GCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCt                                                                                           >  3:116426/1‑138 (MQ=255)

                                                   aTAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAA‑GCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCt                                                                                           >  1:245408/1‑137 (MQ=255)

                                                      aaTCGGGCGCGGGGGAGGGGGGGGATCGGGGTAACTTTCCAGCACTGGGCCCAGTTGGTAA‑GCCCCCCCGTATGGTAGTTATCAACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGAt                                                                                      <  5:141285/139‑1 (MQ=255)

                                                          cggcgCCGGTGCGGGTGGGGCGCGCGGTATCATTGCCCCACTGGGGCCAGATGGTAC‑CCCCCCCCTTAGCGTTTTAATCAACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAgg                                                                                   <  5:4314/134‑1 (MQ=255)

                                                                gggTGTGGGTGGGGCGCGCGTTACCATTGCAGCACTGGGGCCAGTTGGAAA‑GCCCTCCCTTAGCGTATTTATATACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCa                                                                            <  3:227665/138‑1 (MQ=255)

                                                                gggTGCGGGTGGGGCTCGTGTATTCATTGCAGCAGTGGGGCATGATGGTAC‑GCCCGCCCTTAGCGTTGTTAACTCCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCa                                                                            <  3:182685/138‑1 (MQ=255)

                                                                cggggcgCGGGGGGCTCGGGTTATCATTGCAGCACTGGGGCCATTTGGTAA‑GCCCTCCCGTATCGTAGTTAACTACACGACGGGGAGTCAGGCAACTATGGGTGAACGAAATAGACAGATCGATGAGATAGGTGCCTCa                                                                            <  6:73983/133‑1 (MQ=255)

                                                                 ggTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAA‑GCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCAc                                                                           >  1:96808/1‑139 (MQ=255)

                                                                  gTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAA‑GCCCTCCCGTATCGTAGTTATCTACACGCCGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACt                                                                          >  8:34826/1‑139 (MQ=255)

                                                                  gTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAA‑GCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACt                                                                          >  1:175221/1‑139 (MQ=255)

                                                                      gCGGGGGGCGCGCGTTATATTAGCAGCACGGGGGCCAGTTGGAAA‑GCCCTCCGGTATGGTAGTAATCAACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGAtt                                                                      <  5:79761/139‑1 (MQ=255)

                                                                        gggggTCTCGCGGTATTTTGCCAGCCGGGGGGCATTATGGTAC‑GCCCTCCCTTTTGTTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTaa                                                                    <  7:128358/137‑1 (MQ=255)

                                                                           ggTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAA‑GCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCa                                                                 >  1:52318/1‑139 (MQ=255)

                                                                           ggTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAA‑GCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCa                                                                 >  1:187531/1‑139 (MQ=255)

                                                                               tcGCGGTATCATTGCATAACTGGTGACAGATGGTAA‑GCCCTCCAGTATCGTAGTTATCTACACGACGGTGAGTCAGGCAACTATGGATGAACGAAATATACAGATCGCTGAGATAGGTGACTCACTGATTAAGCATTgt                                                             >  6:226771/1‑138 (MQ=255)

                                                                                gggggTATCATTGCAGCCGGGGGGCCAGTTGGAAC‑GCCCTCCCTTCTCGTATTTATACACACGACGGGGAGTCAGGCAACAATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGt                                                            <  4:251827/136‑1 (MQ=255)

                                                                                ggctttATCATTCCAGCAGGGGGGCCTTATGTTAA‑GCCCGCCCGTATCTTAGTTACAAACACGACGGGGAGGCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGt                                                            <  5:203603/134‑1 (MQ=255)

                                                                                cggtgTATCATTGCCGCACGGGGCCCCGATGGAAA‑GCCCCCCCGTATGTTAGTTACCAACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGt                                                            <  7:74113/135‑1 (MQ=255)

                                                                                       tCATTGCAGCACTTGGGCCAGATGGTAA‑GCCCTCACGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGAAGAAAGAAATAGACAGATCGCTGAGATAGGTCCCTCACGGATAAAGCATTGGTAACTGt                                                      >  4:56911/1‑138 (MQ=255)

                                                                                       tCATTGCAGCACTGGGGCCAGATGGTAA‑GCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGt                                                      >  6:2693/1‑138 (MQ=255)

                                                                                            gccccaCTGGGGCCAGTTGGTAA‑GCCCTCCCGTATCGTAGTTAGCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGAcc                                                <  4:107167/135‑1 (MQ=255)

                                                                                            gcagccCGGGGGCTTTATGATAA‑GCACTCCCTTATCGTTTTTTTCACCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGAcc                                                <  2:207756/139‑1 (MQ=255)

                                                                                            gcagcaGGGGGGCAAGATGGTAA‑GCCCTCCCGATTCTTAGTTATCTACACGACGGGGAGTCAGGAAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGGGCCTCACTGATTAAGCATTGGTAACTGTCAGAc                                                 <  2:227841/138‑1 (MQ=255)

                                                                                            cccgcaCGGGGCCCAGA‑GGTAACGCCCACCCTTATCGTTTTTATCACCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGAc                                                 <  4:83358/135‑1 (MQ=255)

                                                                                               gcaCTGGGGCCAGATGGTAA‑GCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGACCGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAg                                             >  6:210945/1‑139 (MQ=255)

                                                                                               gcaCTGGGGCCAGATGGTAA‑GCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAg                                             >  8:83271/1‑139 (MQ=255)

                                                                                               gcaCTGGGGCCAGATGGTAA‑GCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAg                                             >  8:274669/1‑139 (MQ=255)

                                                                                                 ccggggcCCCGATGGAAA‑CCCCCCCCTTACCGTATTAACCAACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGtt                                           <  5:97686/136‑1 (MQ=255)

                                                                                                 acgggggCCCGATGGTAA‑GCCCTCCCGTATCGCAGTAATCAACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGtt                                           <  8:67679/136‑1 (MQ=255)

                                                                                                   tGGGGCCTTATGGTAA‑GCCCCCCCGTATCTTAGTTATATACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                         <  3:67650/139‑1 (MQ=255)

                                                                                                   tGGGGCCAGATGGTAA‑CCCCTCCCTTATCGTAGTAAACACCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                         <  7:261511/139‑1 (MQ=255)

                                                                                                   tGGGGCAAGTTGGAAA‑GCCGGCCCGTATCGTAGTAATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                         <  8:190257/139‑1 (MQ=255)

                                                                                                   tGGGGACATATGGTAC‑CCCCACCGGTAGCTTAGTAATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                         <  1:14945/139‑1 (MQ=255)

                                                                                                   ggcgcccAGAGGGTAC‑CCCCGCCCGTATCGTAGTTAACAACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                         <  5:257046/134‑1 (MQ=255)

                                                                                                   gaggcccAGCTGGTCA‑GCCCGCGCGTATAGTAGTTCTCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                         <  2:177173/134‑1 (MQ=255)

                                                                                                    ggggCCTGTTGGTAC‑GCCCCCCCGTATCGTATTTATCAACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTAc                                        <  1:204021/139‑1 (MQ=255)

                                                                                                     gggCCTGATGGAAA‑CCCCTCCCGTAGCGAAGTAAACTCCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                         <  3:284899/137‑1 (MQ=255)

                                                                                                     gggCCAGATGGTAA‑GCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCCACTATGGATGAACGAAATAGACAGATCGCGGAGATAGGTGCCTCACTGAGTAAGCATTGGTAACTGTCAGACCTCGTTTagt                                       >  8:139620/1‑137 (MQ=255)

                                                                                                     gggCCAGATGGTAA‑GCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAAGAATGGATGAACGAAAAAGACAGATCGCTGAGATAGCTGCCTCACTGATTAAGCATTGCTAACTGTCAGACCAAGTTTACt                                       >  6:171518/1‑139 (MQ=255)

                                                                                                     gggCCAGATGGTAA‑GCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGt                                                      >  1:22725/1‑124 (MQ=255)

                                                                                                     gggCCAGATGGTAA‑GCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTAc                                        >  2:187189/1‑138 (MQ=255)

                                                                                                     gggCCAGATGGTAA‑GCCCTCCCGTATCGTAGTTATCAACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                         <  5:105508/137‑1 (MQ=255)

                                                                                                     gggCCAGATGGTAA‑GCCCTCCCGTATCGAAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATCAACGAAATAGACAGATCCCTGAGATAGGTGCCTCACTGCTTAAGCATTGGTAACTGTCATCCCAAGTTTAc                                        >  2:125663/1‑138 (MQ=255)

                                                                                                     ggcccAGAGGGCAC‑GCCCTCCCGTCGCGTAGAAATCAACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                         <  1:33577/134‑1 (MQ=255)

                                                                                                          agttGGTAA‑CCCCCCCCTTATCGTAGTTAACAACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCatat                                  <  3:267552/136‑1 (MQ=255)

                                                                                                          aGATGGTAA‑GCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCata                                   >  8:48168/1‑138 (MQ=255)

                                                                                                          aGATGGTAA‑GCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCata                                   >  4:274330/1‑138 (MQ=255)

                                                                                                           gATGGTAT‑CACCTCCAGTATATTAGTTTTCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCatata                                 <  4:194318/139‑1 (MQ=255)

                                                                                                           cttGGTAA‑GCCCCCCCGTATCGTAGTTATCACCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCatata                                 <  7:159274/137‑1 (MQ=255)

                                                                                                            aTGGTAA‑GCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGAc                                                 <  3:103389/122‑1 (MQ=255)

                                                                                                            aTGGTAA‑GCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCatata                                 >  2:39126/1‑138 (MQ=255)

                                                                                                               gTAA‑GCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACt                             >  1:71698/1‑139 (MQ=255)

                                                                                                                 aa‑GCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACtt                            >  4:207669/1‑138 (MQ=255)

                                                                                                                      ccTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAgat                       <  6:56011/139‑1 (MQ=255)

                                                                                                                      ccTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAgat                       <  8:232228/139‑1 (MQ=255)

                                                                                                                       cTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAgatt                      >  1:102191/1‑139 (MQ=255)

                                                                                                                         cccGTATCGTTGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCaa                                              >  3:248295/1‑113 (MQ=255)

                                                                                                                         cccGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGAAAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAgattga                    >  7:290339/1‑139 (MQ=255)

                                                                                                                                  tAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGAt                                                                                      >  7:179382/1‑64 (MQ=255)

                                                                                                                                  tAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGt                                                      >  8:173409/1‑96 (MQ=255)

                                                                                                                                  tAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGCCCAAGTTTACTCATATATACTTTAGATTGATTTAAAACt           >  5:225835/1‑139 (MQ=255)

                                                                                                                                  tAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTc                                      <  1:27235/112‑1 (MQ=255)

                                                                                                                                  tAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCatat                                  <  3:143698/116‑1 (MQ=255)

                                                                                                                                  tAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACt           >  2:272888/1‑139 (MQ=255)

                                                                                                                                  tAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACt           >  6:123043/1‑139 (MQ=255)

                                                                                                                                  tAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACt           >  3:91663/1‑139 (MQ=255)

                                                                                                                                  tAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACt           >  4:149783/1‑139 (MQ=255)

                                                                                                                                    gTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACtt          >  2:255784/1‑138 (MQ=255)

                                                                                                                                       aTCTCCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                         <  4:169488/104‑1 (MQ=255)

                                                                                                                                       aTCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGt                                                            >  7:88870/1‑85 (MQ=255)

                                                                                                                                       aTCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGtt                                           >  7:195958/1‑102 (MQ=255)

                                                                                                                                       aTCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCAtt      >  5:177286/1‑139 (MQ=255)

                                                                                                                                         cTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCAtttt    >  5:127161/1‑139 (MQ=255)

                                                                                                                                         cTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCAtttt    >  4:226665/1‑139 (MQ=255)

TTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAA‑GCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTA  >  pSIJ663_pHT315_araE_xy/4511‑4787

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`