BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	pSIJ663_pHT315_araE_xy	5,173	T→G	6.3%	intergenic (+442/–)	ampR → / –	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	pSIJ663_pHT315_araE_xy	5,173	0	T	G	6.3%	6653.3 / 92.6	3285	intergenic (+442/–)	ampR/–	–/–
Reads supporting (aligned to +/- strand): ref base T (1493/1502); new base G (156/111); total (1654/1631)
Fisher's exact test for biased strand distribution p-value = 7.31e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 3395 total aligned reads displayed.

ATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGC  >  pSIJ663_pHT315_araE_xy/5035‑5311

agaCCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGACAGTGGCGATAAGTCGTGTCTTACCGGGt                                                                                                                                            <  5:251323/137‑1 (MQ=255)

aTACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGt                                                                                                                                            >  3:74356/1‑139 (MQ=255)

aTACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGt                                                                                                                                            >  5:148431/1‑139 (MQ=255)

    cAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATGCTGTTACCAGTGGCTGGTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTTGa                                                                                                                                        <  4:134287/139‑1 (MQ=255)

    cAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGa                                                                                                                                        <  3:97341/139‑1 (MQ=255)

      aaTACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACt                                                                                                                                      <  1:189203/139‑1 (MQ=255)

      aaTACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACt                                                                                                                                      <  7:287922/139‑1 (MQ=255)

      aaTACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACt                                                                                                                                      <  8:222819/139‑1 (MQ=255)

          cTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCaa                                                                                                                                   <  5:232829/138‑1 (MQ=255)

             ttcTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGAc                                                                                                                                >  6:101748/3‑138 (MQ=255)

             ttcTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGAc                                                                                                                                >  2:121453/3‑138 (MQ=255)

             ttcTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGGTGGACTCAAGAc                                                                                                                                >  3:57816/3‑138 (MQ=255)

                ttCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGa                                                                                                                              >  6:297280/1‑137 (MQ=255)

                  cTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTAGATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGAt                                                                                                                             <  2:201886/136‑1 (MQ=255)

                  cTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGCCTCAAGACGATAGt                                                                                                                          >  3:284794/1‑139 (MQ=255)

                  cTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGAt                                                                                                                             <  7:117569/136‑1 (MQ=255)

                  cTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGt                                                                                                                          >  1:212672/1‑139 (MQ=255)

                     gtgtAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTa                                                                                                                        <  4:24053/138‑1 (MQ=255)

                     gtgtAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTa                                                                                                                        <  4:113979/138‑1 (MQ=255)

                     gtgtAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTAc                                                                                                                       >  7:22298/1‑139 (MQ=255)

                     gtgtAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGATAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGTACTCAAGACGATAGTTa                                                                                                                        <  7:201912/138‑1 (MQ=255)

                          gCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGa                                                                                                                   >  7:96659/1‑138 (MQ=255)

                           ccGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATa                                                                                                                 <  5:74311/139‑1 (MQ=255)

                                gTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGCTAAGTCGTGTCTTACCGGGGTGGGCGCAAGACGAGAGTTTCCCGATGAGgca                                                                                                            >  1:101415/1‑138 (MQ=255)

                                gTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGa                                                                                                                                        >  8:141987/1‑111 (MQ=255)

                                    ggCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAg                                                                                                         <  1:1229/138‑1 (MQ=255)

                                      ccaccaCTTCAACAACTCTGTAGCACCGCCTACATACCTCGCTCAGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGGCGGGTCTTACCGGGTTGGACTCAAGACGAAAGTTACCGGATAAGGCACAGCgg                                                                                                      <  1:41240/139‑1 (MQ=255)

                                       caccacTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGt                                                                                                     <  5:18672/139‑1 (MQ=255)

                                          cacTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGt                                                                                                                                            >  4:276249/1‑97 (MQ=255)

                                                       ctGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACgggggg                                                                                     >  7:79179/1‑139 (MQ=255)

                                                          tAGCACCGGCTACATACCTCGAGCTGATAATCCTGTTTCCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGGTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTTAACGGGGGGt                                                                                    <  7:265038/137‑1 (MQ=255)

                                                               ccGCCTACATACCTCGCTCTGCTGATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTGGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGca                                                                             <  3:174826/139‑1 (MQ=255)

                                                                cGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACgggg                                                                                       >  1:84353/1‑128 (MQ=255)

                                                                 gCCTACATACCTCGCTCTGCTTATCCTGTTAGCAGTGGCGGCTGCCAGTGGCGATAAGTCGTGGCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGcaca                                                                           <  1:263918/139‑1 (MQ=255)

                                                                 gCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTTGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTTAACGGGGGGTTCGTGcaca                                                                           <  1:64345/139‑1 (MQ=255)

                                                                 gCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGcaca                                                                           <  4:148812/139‑1 (MQ=255)

                                                                 agagaCAGACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCGGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGcaca                                                                           <  2:270110/135‑1 (MQ=255)

                                                                  ccTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTTCCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGcaca                                                                           >  7:62554/1‑138 (MQ=255)

                                                                  ccTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTa                                                                                                                        >  4:199531/1‑93 (MQ=255)

                                                                  ccTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTa                                                                                                                        <  3:151673/93‑1 (MQ=255)

                                                                  ccTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGCACGGGGGGTTCGTGcacac                                                                          >  8:240617/1‑139 (MQ=255)

                                                                  ccTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGcacac                                                                          >  6:170822/1‑139 (MQ=255)

                                                                  ccTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGcacac                                                                          >  7:138098/1‑139 (MQ=255)

                                                                  ccTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGcacac                                                                          >  3:225928/1‑139 (MQ=255)

                                                                  ccTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGGTCGTGcacac                                                                          >  4:9304/1‑139 (MQ=255)

                                                                  ccTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGGTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGcacac                                                                          >  6:238806/1‑139 (MQ=255)

                                                                  ccTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGCTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCt                                                                                               <  3:266306/118‑1 (MQ=255)

                                                                  ccTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCCGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGcacac                                                                          >  4:5231/1‑139 (MQ=255)

                                                                      cATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGc                                                                       <  3:246517/138‑1 (MQ=255)

                                                                      cATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGc                                                                       <  8:11725/138‑1 (MQ=255)

                                                                      cATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGc                                                                       <  8:199360/138‑1 (MQ=255)

                                                                           cTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCCAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGATCGGGGGGTTCGTGCACACAGCCCAGCt                                                                 >  8:157707/1‑139 (MQ=255)

                                                                           cTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCt                                                                 >  2:223947/1‑139 (MQ=255)

                                                                            tCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCtt                                                                >  3:136297/1‑139 (MQ=255)

                                                                            tCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTGCGTGCACACAGCCCAGCtt                                                                >  6:58449/1‑139 (MQ=255)

                                                                             cGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTa                                                                                                                        <  4:70820/82‑1 (MQ=255)

                                                                             cGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTa                                                                                                                        <  6:216616/82‑1 (MQ=255)

                                                                             cGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGgcac                                                                                                           <  2:156754/95‑3 (MQ=255)

                                                                             cGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACgggggg                                                                                     <  7:16016/117‑1 (MQ=255)

                                                                             cGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTAGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGcc                                                                      >  8:178058/1‑132 (MQ=255)

                                                                                tctGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCg                                                                                 <  6:277378/118‑1 (MQ=255)

                                                                                tctGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCg                                                                                 >  5:277378/1‑118 (MQ=255)

                                                                                    cTAATCCTTTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGa                                                         <  5:167133/138‑1 (MQ=255)

                                                                                    cTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTTCCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGa                                                         <  2:93797/138‑1 (MQ=255)

                                                                                    cTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTGCCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGa                                                         <  1:2357/138‑1 (MQ=255)

                                                                                    cTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGaa                                                        >  7:47139/1‑139 (MQ=255)

                                                                                    cTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGaa                                                        <  4:254575/139‑1 (MQ=255)

                                                                                    cTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGa                                                         <  2:182810/138‑1 (MQ=255)

                                                                                    cTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGCGCACACAGCCCAGCTTGGAGCGaa                                                        <  6:150936/139‑1 (MQ=255)

                                                                                        tCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGAc                                                    <  5:30391/139‑1 (MQ=255)

                                                                                        tCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGAc                                                    <  5:209498/139‑1 (MQ=255)

                                                                                          cTGTTACGAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCt                                                  <  7:241607/139‑1 (MQ=255)

                                                                                            gTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTTCCGGGTTGGACTGCACACGATAGGTACCGGTTAAGGCACCGCGGTCGGGCTGAACGGGGGGTTCGTGCCCACAGCCCCGCTTGGAGCGCAAGCCCTac                                                >  4:218595/1‑139 (MQ=255)

                                                                                            gTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGcaca                                                                           <  8:46264/112‑1 (MQ=255)

                                                                                              tACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGa                                                         >  1:286382/1‑128 (MQ=255)

                                                                                              tACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCAAGCTTGGAGCGAACGACCTacac                                              >  6:131739/1‑139 (MQ=255)

                                                                                                  aGTGTCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCACGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGaa                                          <  3:199730/139‑1 (MQ=255)

                                                                                                   gTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGGTGGACTCAAGACGCTAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACCCAGCCCAGCTGGGAGCGTACGCCCTACACAGAAc                                         >  1:172069/1‑139 (MQ=255)

                                                                                                    tGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTa                                                                                                                        <  2:34597/59‑1 (MQ=255)

                                                                                                      gctgctGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTga                                      <  5:109024/139‑1 (MQ=255)

                                                                                                      gctgctGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTga                                      <  5:53479/139‑1 (MQ=255)

                                                                                                       ctgctgCCAGTGGCGATCAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTgag                                     <  1:14811/139‑1 (MQ=255)

                                                                                                        tgctgcCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTgaga                                    <  8:83256/139‑1 (MQ=255)

                                                                                                          ctgcCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTgaga                                    <  6:196591/137‑1 (MQ=255)

                                                                                                          ctgcCAGTGGCGATAAGTCGTGGCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCGGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTgaga                                    <  2:52747/137‑1 (MQ=255)

                                                                                                           tgcCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGca                                                                             >  3:39290/1‑95 (MQ=255)

                                                                                                              cAGTGGCGATAAGGCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAATGCACAGCGGTCGGGCTGAACGGGGGGTGCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTa                              <  2:78046/139‑1 (MQ=255)

                                                                                                                gTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTa                              <  8:282533/137‑1 (MQ=255)

                                                                                                                 tGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGc                                                                       >  2:104890/1‑95 (MQ=255)

                                                                                                                  ggCGATAAGTCGTGGCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAg                           <  7:182746/138‑1 (MQ=255)

                                                                                                                   gCGATAAGTCGTGTCTTCCCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCg                         <  8:264173/139‑1 (MQ=255)

                                                                                                                   gCGATAAGGCGTGTCTTACCGGGGTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCg                         <  6:85364/139‑1 (MQ=255)

                                                                                                                    cGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGt                                                                                >  3:117290/1‑83 (MQ=255)

                                                                                                                    cGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGCACGACCTACACCGCACTGAGATACCTACAGCGt                        >  6:111852/1‑139 (MQ=255)

                                                                                                                     gATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCCGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGt                        >  5:126067/1‑138 (MQ=255)

                                                                                                                             gtgtCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTgaga                                    >  2:243443/1‑118 (MQ=255)

                                                                                                                               gtCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATgaga             <  6:256941/139‑1 (MQ=255)

                                                                                                                                  ttACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGCCCTACACCGAACTGAGATACCTACAGCGGGAGCTATGAGAAAg          >  4:33711/1‑139 (MQ=255)

                                                                                                                                     ccGGGTGTGACTCAAGACGATCGTAACCGGTTAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCCAACGACCTACACCGAACTGAGAAACCTACAGCGTGAGATATGAGAAAgcgc       <  4:1747/139‑1 (MQ=255)

                                                                                                                                          ttGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGAt                                   >  8:63585/1‑106 (MQ=255)

ATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGC  >  pSIJ663_pHT315_araE_xy/5035‑5311

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`