BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	pSIJ663_pHT315_araE_xy	6,676	T→G	6.4%	intergenic (+1945/–)	ampR → / –	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	pSIJ663_pHT315_araE_xy	6,676	0	T	G	6.4%	4945.1 / 48.7	1749	intergenic (+1945/–)	ampR/–	–/–
Reads supporting (aligned to +/- strand): ref base T (872/733); new base G (4/138); total (878/871)
Fisher's exact test for biased strand distribution p-value = 8.02e-39
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1765 total aligned reads displayed.

GCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAAC  >  pSIJ663_pHT315_araE_xy/6538‑6814

 ccATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTAGGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGATg                                                                                                                                           <  5:247853/139‑1 (MQ=255)

 ccATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTg                                                                                                                                           >  4:229610/1‑139 (MQ=255)

 ccATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTg                                                                                                                                           <  3:272408/139‑1 (MQ=255)

 ccATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTg                                                                                                                                           <  4:67704/139‑1 (MQ=255)

 ccATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTg                                                                                                                                           <  2:6566/139‑1 (MQ=255)

 ccATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTg                                                                                                                                           <  6:168231/139‑1 (MQ=255)

  cATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTgg                                                                                                                                          >  3:233534/1‑139 (MQ=255)

    tGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGa                                                                                                                                        >  5:129590/1‑139 (MQ=255)

    tGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGa                                                                                                                                        >  5:81469/1‑139 (MQ=255)

     gTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACGTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGa                                                                                                                                        >  2:101867/1‑138 (MQ=255)

      ttGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACa                                                                                                                                      <  4:107914/139‑1 (MQ=255)

      ttGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACa                                                                                                                                      <  8:186816/139‑1 (MQ=255)

      ttGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACa                                                                                                                                      <  2:194536/139‑1 (MQ=255)

      ttGATGAGTCAAACCAAAGAAAACCCCCTTCGCATGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGAc                                                                                                                                       <  6:197515/138‑1 (MQ=255)

        gatgaGTCAAACCAAAGAAAACCCCCTACGCCAGGGGATTATTTTGTGGCGTACCCTCGCCGGAAAGATAAGCCAGAAACCAAACTGGCTCGGCACTTGAATCACCCTGTTGAGCTGCACCGAATAAACCTGGGACAtt                                                                                                                                    >  1:235835/1‑139 (MQ=255)

         atgaGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGCCATTg                                                                                                                                   >  3:166877/1‑139 (MQ=255)

         atgaGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTg                                                                                                                                   >  4:124168/1‑139 (MQ=255)

            aGTCAAACCAAAGAAAACCCCCTTCGGCTGGGGATTATTGTGTGGCGGACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCt                                                                                                                                 <  8:31387/138‑1 (MQ=255)

              tCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAg                                                                                                                              >  6:40022/1‑139 (MQ=255)

              tCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAg                                                                                                                              >  7:7265/1‑139 (MQ=255)

               cAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAgg                                                                                                                             <  6:223740/139‑1 (MQ=255)

                aaaCCAAAGAAAACCCCCTTCGGCTGTGGATTATTGTGTCGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCACACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAgg                                                                                                                             <  5:281937/138‑1 (MQ=255)

                aaaCCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGNAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAgg                                                                                                                             <  5:297461/138‑1 (MQ=255)

                aaaCCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCCCTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGc                                                                                                                            >  4:259143/1‑139 (MQ=255)

                aaaCCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACa                                                                                                                                      <  8:53652/129‑1 (MQ=255)

                aaaCCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAgg                                                                                                                             <  6:251820/138‑1 (MQ=255)

                aaaCCAAAGAAAACACCCTTCGCCAGGGGATTATTTTGTGGCGTACGCTAGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTAATCAGCCTGTTGAGATGCAGCGATTAAAGCACGGACAATGCTCAGGc                                                                                                                            >  1:120205/1‑139 (MQ=255)

                       aGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTaa                                                                                                                     >  4:144093/1‑139 (MQ=255)

                       aGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTaa                                                                                                                     >  1:206926/1‑139 (MQ=255)

                            aCCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTcg                                                                                                                <  2:168074/139‑1 (MQ=255)

                            aCCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTcg                                                                                                                <  6:91884/139‑1 (MQ=255)

                               cccTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCgg                                                                                                             >  7:178966/1‑139 (MQ=255)

                                ccTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACa                                                                                                                                      <  7:91451/113‑1 (MQ=255)

                                 cTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGcc                                                                                                           <  8:209717/139‑1 (MQ=255)

                                 cTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGcc                                                                                                           <  2:41230/139‑1 (MQ=255)

                                     gCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAAt                                                                                                       <  8:72422/139‑1 (MQ=255)

                                          gggATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGa                                                                                                  >  7:192574/1‑139 (MQ=255)

                                          gggATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCCGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGa                                                                                                  >  7:226457/1‑139 (MQ=255)

                                                   gtgggGCGTACGCGCGCCGTAAAGAAACGGCACAAACAAAACTGGCTCGGCACTTTCATCAGCCTTTTGAGCTGCAGCTATAAAAGCTGGGACATTGCTCAGGCCGTTTAATTTCGCGGCCCAATCCAGACACGACGCt                                                                                         <  6:143073/139‑1 (MQ=255)

                                                    tgtgGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACGCTg                                                                                        >  5:49293/1‑139 (MQ=255)

                                                     gtgGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGCCACGACGCTGc                                                                                       >  3:186980/1‑139 (MQ=255)

                                                      tgGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTg                                                                                                                                           >  3:131611/1‑86 (MQ=255)

                                                      tgGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTg                                                                                                                                           >  5:150864/1‑86 (MQ=255)

                                                      tgGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGAc                                                                                                                                       <  3:52294/90‑1 (MQ=255)

                                                      tgGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACGCTGCa                                                                                      >  1:101726/1‑139 (MQ=255)

                                                      tgGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACGCTGCa                                                                                      >  3:147564/1‑139 (MQ=255)

                                                      tgGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACGCTGCa                                                                                      >  7:565/1‑139 (MQ=255)

                                                      tgGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACCCTGCa                                                                                      >  2:253136/1‑139 (MQ=255)

                                                         cGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAgg                                                                                                                             >  1:182500/1‑97 (MQ=255)

                                                           tACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCCCTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGcc                                                                                                           >  8:225189/1‑113 (MQ=255)

                                                           tACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGccc                                                                                                          >  5:116110/1‑114 (MQ=255)

                                                           tACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGcc                                                                                                           <  1:35125/113‑1 (MQ=255)

                                                           tACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGcc                                                                                                           <  2:123020/113‑1 (MQ=255)

                                                           tACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGcc                                                                                                           <  2:285746/113‑1 (MQ=255)

                                                           tACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAg                                                                                 >  7:103245/1‑139 (MQ=255)

                                                           tACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAg                                                                                 >  5:10075/1‑139 (MQ=255)

                                                           tACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAg                                                                                 >  5:180639/1‑139 (MQ=255)

                                                           tACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAg                                                                                 >  3:294035/1‑139 (MQ=255)

                                                              gcgcGCCGTAAAGATACGCCACAAACCAAACTGGCTCGGAAATTTCATCCGCGTGTGGAGCTGCCGCGATTAACGCGGGGACTTGGCTCTGGCCGTTTAATTGCGCGGCCCAATCCACACACGACGCTGCACTCAGCAt                                                                              <  6:271381/136‑1 (MQ=255)

                                                                  gCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCCGCCTGTTGAGCTGCAGCGATTAACGCTGGGACATTGCTCAGGCCGGTTATTTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATcaca                                                                          <  6:122341/139‑1 (MQ=255)

                                                                  gCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATca                                                                            >  4:41060/1‑137 (MQ=255)

                                                                  gCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATca                                                                            >  2:95572/1‑137 (MQ=255)

                                                                   ccGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACGCTGCCCTCAGCATCACAg                                                                         >  1:261288/1‑139 (MQ=255)

                                                                        aaGATACGGCAGAACCCAAACTGGCGCGGCACTTCCAGCAGCCTTTTGAGAGGCAGCTATTAAAGCTGGGACATTGCTCAGGCGGTTTAATTTCGCGGCCCAATCCAGCCACGACGCTGCACTCAGCATCACAGACAtt                                                                    <  7:13102/139‑1 (MQ=255)

                                                                            tAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAAGCCAGACACGACGCTGCACTCAGCATCCCAGACATTAAAt                                                                >  2:150002/1‑139 (MQ=255)

                                                                              aGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGACTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCGGGGTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGc                                                              >  1:287039/1‑139 (MQ=255)

                                                                               ggCACAACCCAACCGGGCGCGCCACTTTCAACCGCGTGTGGAGCGGCAGCGATTAAAGCGGGGACATGCCTCAGGCCGGTTAATTGCGCGGCCCAACCCACACACGACGCTGCACTCAGCATCACAGACATTAAATGcc                                                             <  7:38937/139‑1 (MQ=255)

                                                                                    aaaCCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGcc                                                                                                           <  1:145437/88‑1 (MQ=255)

                                                                                    aaaCCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGt                                                        >  5:96460/1‑139 (MQ=255)

                                                                                    aaaCCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGt                                                        >  5:20542/1‑139 (MQ=255)

                                                                                    aaaCCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGt                                                        >  4:197049/1‑139 (MQ=255)

                                                                                    aaaCCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGt                                                        >  5:231242/1‑139 (MQ=255)

                                                                                    aaaCAAAACGGGCTCGGCACTTTCAGCCGCCTGTTGCGCGGCAGCTATAAAAGCTGGTACATTGCTCTGGCCGTTAAATTTCGCGGCCCAATCCACCCACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGt                                                        <  6:107222/139‑1 (MQ=255)

                                                                                          aaCTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACGCTGCCCTCAGCATCACAGACATTAAATGCCAACGTTGCgg                                                   >  7:279488/1‑138 (MQ=255)

                                                                                          aaCTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCgg                                                   >  7:247134/1‑138 (MQ=255)

                                                                                           aCTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCt                                                                                                                                 <  8:174901/59‑1 (MQ=255)

                                                                                               gcccGGCACTTTCATCCGCCTGTGCAGCGGCAGCGATAAACGCGGGGACATTGCGCCGGCCGGTAAATTCCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAgcgc                                             <  1:79073/136‑1 (MQ=255)

                                                                                                 tCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTTGGACATTGCTCAGGCCGGTTAATTTCGCTGcc                                                                                                           <  8:103513/75‑1 (MQ=255)

                                                                                                    gCACTTTCAGCCGCCTGTTGACCGGCAGCGATAAAAGCGGGGCCATTGCGCCGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGc                                        <  4:85193/139‑1 (MQ=255)

                                                                                                      ccTTACATCACCCTGAGGAGCTGCAGCGATTAACGCGGGGACATGGCGCCGGCGGGTAAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCaa                                      <  8:158045/138‑1 (MQ=255)

                                                                                                      catttCATCAGCCTGTTGAGCTGCAGCGATTAAAGCGGGGACATTGCCCAGGCCGGTTAATTTCGCGGCCCAATCCACACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCaa                                      <  6:69915/137‑1 (MQ=255)

                                                                                                      aCTTTCATCCGCCTGTTCAGCTGAAGCCATTAAAGCTGGCACATTGCTCAGCCCGGTTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCaa                                      <  3:76299/139‑1 (MQ=255)

                                                                                                      aCTTTCAGCAGCCTGTTGTGCTGCAGCGATTAACGCGGGGAAATTCGTCCGGCCGGTTAATTGCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCaa                                      <  3:256070/139‑1 (MQ=255)

                                                                                                         ttCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAg                                   >  8:14454/1‑139 (MQ=255)

                                                                                                                 ccTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTAc                           >  7:266594/1‑139 (MQ=255)

                                                                                                                  cTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACACTACGCTGCACTAATCATCACAGACATTAAATGACAACGTTTCGGTAGCGAATGGAAAAACCTATGTACg                          >  1:157399/1‑139 (MQ=255)

                                                                                                                    ggtGAGCTGCTTCTATTAATGCGGGCACTTTGCTCCGGCCGGTTAATTTGGCGGCACAAGCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACgg                         <  7:146907/136‑1 (MQ=255)

                                                                                                                    gTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTAcctg                        >  3:93567/1‑136 (MQ=255)

                                                                                                                     ttccgCTGCAGCGATTAACGCGGGCACATTGCTCAGGCCGGTAAATTGCGCGGCCCAATCCACCCACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACgg                         <  7:159838/133‑1 (MQ=255)

                                                                                                                     ttGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACgg                         <  5:261821/137‑1 (MQ=255)

                                                                                                                     ttGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGgcg                       >  8:245709/1‑139 (MQ=255)

                                                                                                                          cTGCAGCGATTAAAGCTGGGACATTGCTCCGGCCGGTGAATTTCGCGGCCCCATCCAGTCACGCCGCTGCACTCAGCATCACGGACATTAACTGCCACCGTTGCGGTAGCGCATGGCAGGCGCTGTGTACGGCGCGTTc                  >  6:291840/1‑139 (MQ=255)

                                                                                                                             cAGCTATTAACGCGGCGACATGGCCCTGGCCGGTCTATTGCGCGGCCAAACCCACACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTgg               <  7:210997/139‑1 (MQ=255)

                                                                                                                             cAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTgg               >  1:47272/1‑139 (MQ=255)

                                                                                                                               gCGATTAAAGCGGGGACATTGGTCTGGGCGTTTAATTTCGCGGCCAATGCCCCCCACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGgct             <  3:112384/139‑1 (MQ=255)

                                                                                                                                agATTAATTCTGGGACATGTCCATGGCCGGTTAATTTCGCGGCCAAATCCAGCCACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGATGCGGTAGCTCATGGCAAAAGCTATGTACGGCGCTTTCTGgct             <  8:2756/137‑1 (MQ=255)

                                                                                                                                     aaaGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGgcttgctt        >  3:208061/1‑138 (MQ=255)

                                                                                                                                       aGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGgcttgctt        >  4:169314/1‑136 (MQ=255)

                                                                                                                                       aGCGGGCACATTGCTCAGGCGGTTTAATTGCGCGGCCCATTCCAGCCACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAg     <  4:282034/139‑1 (MQ=255)

                                                                                                                                        gCTGGGACATTGCTCAGGCCGGTTAATTTCGGGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGa    <  2:229941/139‑1 (MQ=255)

GCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAAC  >  pSIJ663_pHT315_araE_xy/6538‑6814

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`