BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	pSIJ663_pHT315_araE_xy	6,717	G→C	8.3%	intergenic (+1986/–)	ampR → / –	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	pSIJ663_pHT315_araE_xy	6,717	0	G	C	8.3%	4247.9 / 80.8	1435	intergenic (+1986/–)	ampR/–	–/–
Reads supporting (aligned to +/- strand): ref base G (847/438); new base C (2/136); total (851/584)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1439 total aligned reads displayed.

GGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGG‑TTAA‑TTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCTGACAGCAAAATAGACCCCCGACGTCCCCAGCG  >  pSIJ663_pHT315_araE_xy/6580‑6854

gggATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGG‑TTAA‑TTTCGCGGCCCAATCCAGa                                                                                                                                          >  3:155179/1‑139 (MQ=255)

gggATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGG‑TTAA‑TTTCGCGGCCCAATCCAGa                                                                                                                                          >  7:53896/1‑139 (MQ=255)

gggATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGG‑TTAA‑TTTCGCGGCCCAATCCAGa                                                                                                                                          >  5:249646/1‑139 (MQ=255)

gggATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGG‑TTAA‑TTTCGCGGCCCAATCCAGa                                                                                                                                          >  5:52698/1‑139 (MQ=255)

gggATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGG‑TTAA‑TTTCGCGGCCCAATCCAGa                                                                                                                                          >  3:282034/1‑139 (MQ=255)

gggATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGG‑TTAA‑TTTCGCGGCCCAATCCAGa                                                                                                                                          >  3:40781/1‑139 (MQ=255)

  tttttttGTGGGGCGAACGGTCGCGGTAAAGATACGGCAGAAACCAAACTGGCGCGGCACTTTCATCTGCGTGTTGAGCTCCAGCTATTAAAGCGGGTACTTTGCTCTGG‑CGG‑TTAATTTTCGCGCCCAAACCCCGaca                                                                                                                                        <  2:28908/134‑1 (MQ=255)

  ttttattGTGTGGCGACCGGTCGCCGCAAAGATAAGGCAGAAACCAAACTGGGTCGGAACTTTCATCCGCCTGTTTAGCTGCAGCTATTAAAGCTGGGACATTGCTCCGCCGGT‑TTAA‑TTTCGCGCCCCAATCCCGaca                                                                                                                                        <  6:101402/137‑1 (MQ=255)

     tttggtgggGCTTACGCGCGCCGTAAAGATAAGGCAGAAACCAAACTGGCTCGGAACTTTAATAAGCGTGTTGAGCGGACTCTATTAAAGCTGGGACATTGCTCAGGCCGG‑TAAA‑TTGCGGGGCCCAATCCAGACacg                                                                                                                                      <  8:172329/134‑1 (MQ=255)

            tgGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGG‑TTAA‑TTTCGCGGCCCAATCCAGACACGACGCTGcc                                                                                                                              >  3:7785/1‑138 (MQ=255)

            tgGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGG‑TTAA‑TTTCGCGGCCCAATCCAGACACGACGCTGCa                                                                                                                              >  7:72384/1‑139 (MQ=255)

            tgGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGG‑TTAA‑TTTCGCGGCCCAATCCAGACACGACGCTGCa                                                                                                                              >  3:102794/1‑139 (MQ=255)

            tgGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGG‑TTAA‑TTTCGCGGCCCAATCCAGACACGACGCTGCa                                                                                                                              >  1:101726/1‑139 (MQ=255)

            tgGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGG‑TTAA‑TTTCGCGGCCCAATCCAGACACGACGCTGCa                                                                                                                              >  7:222603/1‑139 (MQ=255)

            tgGCGTACCGTCGCCGCAAATTTCAGGCATAAGCCAACCGGGCGCGCCACTTTCTTCAGCGTGTTGTGCTGCTGCTATTAACGGTGGGACATTGCTCTGGCGGG‑TTAT‑TTTCGCGGCCAAATCCAGACACGACGCTGCa                                                                                                                              <  2:245778/139‑1 (MQ=255)

              gCGTCCGCGCGCCGTAAAGATACGGCATAAACCAACCTGGCTCGGCACTTTCATCCGCCTTTTGAGCGGCAGCGATTAACGCGGGGACATTGCCCCGGCCGT‑TTAA‑TTGCGCGCCCCAAACCACACACGACGCTGCACt                                                                                                                            <  8:36991/139‑1 (MQ=255)

               cGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGG‑TTAA‑TTTCGCGGCCCAATCCAGACACGACGCTGCACTc                                                                                                                           >  5:12668/1‑139 (MQ=255)

                gTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGCCACTTTCATCCGCCTGTTGAGCTGCAGCGATTAACGCTGGGACATTGCTCAGGCCGG‑TTAA‑TTCCGCGGCCCAATCCAGACACGACGCTGCACTCa                                                                                                                          <  3:137770/139‑1 (MQ=255)

                gTACGCCCGCCGTAAAGATAAGGCAGAAACCAACCGGCCGCGCAACTTTCATCAGCCTGTTGCGCTGCTGCGATTAACGCTGGGACATTGCTCCGGCGGT‑TTAA‑TTTCGCGGCCCAATCCAGACACGACGCTGCACTCa                                                                                                                          <  8:5523/139‑1 (MQ=255)

                 tACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGG‑TTAA‑TTTCGCGGCCCAATCCAGACACGCCGCTGCAcctgt                                                                                                                         >  7:139228/1‑135 (MQ=255)

                 tACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGG‑TTAA‑TTTCGCGGCCCAATCCAGACACGACGCTGCACTCAg                                                                                                                         >  7:102685/1‑139 (MQ=255)

                 tACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGG‑TTAA‑TTTCGCGGCCCAATCCAGACACGACGCTGCACTCAg                                                                                                                         >  6:261821/1‑139 (MQ=255)

                 tACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGG‑TTAA‑TTTCGCGGCCCAATCCAGACACGACGCTGCACTCAg                                                                                                                         >  5:10075/1‑139 (MQ=255)

                 tACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGG‑TTAA‑TTTCGCGGCCCAATCCAGACACGACGCTGCACTCAg                                                                                                                         >  7:282206/1‑139 (MQ=255)

                 tACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGG‑TTAA‑TTTCGCGGCCCAATCCAGACACGACGCTGCACTCAg                                                                                                                         >  8:242615/1‑139 (MQ=255)

                 tACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGG‑TTAA‑TTTCGCGGCCCAATCCAGACACGACGCTGCACTCAg                                                                                                                         >  1:42110/1‑139 (MQ=255)

                       cgccgcAAACAAACGGCACAAACCAACCTGGCTCGGCACTTGCATCAGCCTGTTGCGCTGCAGCGATTAACGCTGGGACATTGCCCCGGCCGG‑TAAA‑TTTCGCGGCCCAATCCACACACGACGCTGCACTCAGCATcac                                                                                                                   <  1:199921/139‑1 (MQ=255)

                        gcgtgAAATATAAGGCATAAACCAAACTGGGTCGGCACTTTCATCAGCCTGTTGTGCTGCAGCTATTAACGCTGGGACTTTGCTCCGGCCGT‑TTAT‑TTTCGCGGCCAAATCCAGACACGACGCTGCACTCAGCATcaca                                                                                                                  <  6:85560/135‑1 (MQ=255)

                        gCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGGCATTGCTCAGGCCGG‑TTAA‑TTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATca                                                                                                                    >  8:105909/1‑137 (MQ=255)

                        gCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGG‑TTAA‑TTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATcaca                                                                                                                  >  1:229941/1‑139 (MQ=255)

                                    aGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGG‑TTAA‑TTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGc                                                                                                      >  1:293663/1‑139 (MQ=255)

                                     cgccgAAACCAACCGGGCGCGGCACTTTCATCAGCCTGGTCAGCGGCCGCGATTAACCCTGGGACATGGCCCAGGCCGG‑TTAA‑TTTCGCGGCCCAATCCACCCACGACGCTGCACTCAGCATCACAGACATTAAATGcc                                                                                                     <  7:241333/135‑1 (MQ=255)

                                       cacaacccAAACGGGCTCGGCAGTTTCATCCGCGTGGTGAGCTGCACCGATTAATGCTGGGACATTCCTCCGGCCGG‑TTAA‑TTTCGCGCCCCAATCCACACACGACGCTGCACTCAGCATCACAGACATTAAATGCCaa                                                                                                   <  4:144294/133‑1 (MQ=255)

                                          aaaCCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGG‑TTAA‑TTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGt                                                                                                >  1:1306/1‑139 (MQ=255)

                                                aaCTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGG‑TTAA‑TTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCgg                                                                                           >  3:91676/1‑138 (MQ=255)

                                                     gcacGGCACTTTCATCAGCCTGTTGATATGCCTCTATAAACGGTGGGACATTCAGAAGGCCTG‑TTAA‑TTCCGCGGCCCAATCCAGACACTACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAgcgc                                                                                     <  6:65639/136‑1 (MQ=255)

                                                            aCTTTCATCCCGCTGTTGAGCTGATGCTTTTTACGCTGGGACATTGCTCTCGCCGT‑TAAA‑TTGCCGGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGTTAGCGCATGGcta                                                                              <  2:8115/139‑3 (MQ=255)

                                                            aCTTTCATCAGCCTGTTGAGCTGCTGCGATTAAAGCGGGTACATTGCTCTGGCCGG‑TTAA‑TTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCaa                                                                              <  8:72384/139‑1 (MQ=255)

                                                            aCTTTCATCAGCCTGTGGAGCGCCAGCTATTAAAGCGGGTACATTGCTCTGGCCGG‑TTAA‑TTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCaa                                                                              <  6:127629/139‑1 (MQ=255)

                                                            aCTTACATCACCAGGTTGAGCTGCAGCGATAAAAGCTGGGACATTGCGCTGGCGGG‑TTAA‑TTTCGCGGCCCAACCCACACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCaa                                                                              <  7:165597/139‑1 (MQ=255)

                                                               ttCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGG‑TTAA‑TTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAg                                                                           >  8:14454/1‑139 (MQ=255)

                                                                tcatcaGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGG‑TTAA‑TTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCTTGGCAAAAGc                                                                          >  4:207849/1‑139 (MQ=255)

                                                                 catcaGCGTGTTGCGCGGCAGCTATAAAAGCGGGGACATGGCCCCGCCCGG‑TAAA‑TTTCCCGCCCCAATCCCGCCACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGc                                                                          <  5:283579/138‑1 (MQ=255)

                                                                       ccTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGG‑TTAA‑TTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTAc                                                                   >  4:32001/1‑139 (MQ=255)

                                                                           ttGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGG‑GAAA‑TTTCGCGGCCAACTCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTCCGgcg                                                               >  5:3404/1‑139 (MQ=255)

                                                                                cTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGG‑TTAA‑TTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTc                                                          >  8:187245/1‑139 (MQ=255)

                                                                                   cAGCGATTAAAGCTGGGACATTGCTCAGGCCGG‑TTAA‑TTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTgg                                                       >  1:147736/1‑139 (MQ=255)

                                                                                   cAGCGATTAAAGCTGGGACATTGCTCAGGCCGG‑TTAA‑TTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTgg                                                       >  3:130523/1‑139 (MQ=255)

                                                                                   cAGCGATTAAAGCTGGGACATTGCTCAGGCCGG‑TTAA‑TTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTgg                                                       >  3:115151/1‑139 (MQ=255)

                                                                                   cAGCGATTAAAGCTGGGACATTGCTCAGGCCGG‑TTAA‑TTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTgg                                                       >  7:258753/1‑139 (MQ=255)

                                                                                   cAGCGATTAAAGCTGGGACATTGCTCAGGCCGG‑TTAA‑TTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTgg                                                       >  1:173602/1‑139 (MQ=255)

                                                                                    aGCGATTAAAGCTGGGACATTGCTCAGGCCGG‑TTAA‑TTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGgc                                                      >  1:77975/1‑139 (MQ=255)

                                                                                     gggATTAAAGCTGGGACATTGCTCAGGCCGG‑TTAA‑TTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGgct                                                     <  5:210575/137‑1 (MQ=255)

                                                                                     gCGATTAACGCTGGGACATTGCTCAGGCCGG‑TTAA‑TTCCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTAgct                                                     <  5:200061/139‑1 (MQ=255)

                                                                                     gCGATTAAAGCTGGGACATTGCTCAGGCCGG‑TTAA‑TTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGgct                                                     >  7:94529/1‑139 (MQ=255)

                                                                                       gATTAAAGCTGGGACATTGCTCAGGCCGG‑TTAA‑TTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGgcttg                                                   <  8:282206/139‑1 (MQ=255)

                                                                                        aTTAAAGCTGGGACATTGCTCAGGCCGG‑TTAA‑TTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGgcttgc                                                  >  5:170849/1‑139 (MQ=255)

                                                                                           aaaGCTGGTACATTGCTCAGGCCGG‑TTAA‑TTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGgcttgctt                                                <  8:156841/138‑1 (MQ=255)

                                                                                             aGCTGGGACATTGCTCAGGCCGG‑TTAA‑TTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGgcttgctt                                                >  6:144073/1‑136 (MQ=255)

                                                                                             aGCGGGCACATTGCTCAGGCGGT‑TTAA‑TTGCGCGGCCCATTCCAGCCACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAg                                             <  4:282034/139‑1 (MQ=255)

                                                                                                 gggACATTGCTCAGGCCGG‑TTAA‑TTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAAcc                                         >  2:139367/1‑139 (MQ=255)

                                                                                                  ggACATTGCTCAGGCCGG‑TTAA‑TTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAAccc                                        >  5:233032/1‑139 (MQ=255)

                                                                                                  ggACATTGCTCAGGCCGG‑TTAA‑TTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAAccc                                        >  8:263790/1‑139 (MQ=255)

                                                                                                  ggACATTGCTCAGGCCGG‑TTAA‑TTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAAccc                                        >  4:103719/1‑139 (MQ=255)

                                                                                                  ggACATTGCTCAGGCCGG‑TTAA‑TTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAAcc                                         >  6:244836/1‑138 (MQ=255)

                                                                                                  ggACATTGCTCAGGCCGG‑TTAA‑TTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAAcc                                         >  7:121704/1‑138 (MQ=255)

                                                                                                  ggACATTGCTCAGGCCGG‑TTAA‑TTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGATTGCTTAAGAAccc                                        >  7:204455/1‑139 (MQ=255)

                                                                                                   taCATTGCTCCGGCGGG‑TTAA‑TTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCt                                       <  5:81537/138‑1 (MQ=255)

                                                                                                       ttGCTCAGGCCGG‑TTAA‑TTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGc                                   >  1:270245/1‑139 (MQ=255)

                                                                                                        tGCTCAGGCCGG‑TTAA‑TTTCGCGGCCCAATCCAGaca                                                                                                                                        <  6:270488/37‑1 (MQ=255)

                                                                                                        tGCTCAGGCCGG‑TTAA‑TTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGCCATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTCCGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCt                                  >  6:40905/1‑139 (MQ=255)

                                                                                                        tGCTCAGGCCGG‑TTAA‑TTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCaa                                                                              >  4:254591/1‑95 (MQ=255)

                                                                                                        tGCTCAGGCCGG‑TTAA‑TTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCaa                                                                              <  3:254591/95‑1 (MQ=255)

                                                                                                        tGCTCAGGCCGG‑TTAA‑TTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGACCCCTTCGCt                                  >  4:181619/1‑139 (MQ=255)

                                                                                                        tGCTCAGGCCGG‑TTAA‑TTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCt                                  >  6:212968/1‑139 (MQ=255)

                                                                                                         gCTCAGGCCGG‑TAAA‑TTTCGCGCCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCt                                  <  2:47814/138‑1 (MQ=255)

                                                                                                          ttCAGGCGGT‑TTAA‑TTTCGCGGCCCAATCCAGCCACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCTGa                                <  4:123025/138‑1 (MQ=255)

                                                                                                          cTCCGGCCGG‑TTAA‑TTTCGCGGCCCAATCCACACACGAAGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCTGa                                <  4:77129/139‑1 (MQ=255)

                                                                                                          cTCCGGCCGG‑TAAA‑GTACGCGCCCCAACCCAGCCACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCTGa                                <  3:180236/139‑1 (MQ=255)

                                                                                                            cAGGCCGG‑TTAA‑TTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCTGa                                >  1:60887/1‑137 (MQ=255)

                                                                                                             aGGCCGG‑TTAA‑TTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCTGACAg                             <  4:241738/139‑1 (MQ=255)

                                                                                                             aGGCCGG‑TTAA‑TTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCTGACAg                             >  3:61566/1‑139 (MQ=255)

                                                                                                               gCCGGTTTAA‑TTTCGGGGCCAATTCCCTACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGGTGCGGTAGCGGATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCTGACAg                             <  8:226457/138‑1 (MQ=255)

                                                                                                               gCCGG‑TTAA‑TTTCGCGGCCCAATCCAGCCACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCaa                                                                              <  5:143806/88‑1 (MQ=255)

                                                                                                               gCCGG‑TTAA‑TTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCTGACAg                             <  3:237351/137‑1 (MQ=255)

                                                                                                               gCCGG‑TTAA‑TTCCGCGCCCCAAGCCCGCCACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCTGACAg                             <  4:63477/137‑1 (MQ=255)

                                                                                                                     ttAA‑TTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCTGACAGCaaaa                        >  7:180918/1‑137 (MQ=255)

                                                                                                                     ttAA‑TTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCTGACAGCaaaa                        >  7:153076/1‑137 (MQ=255)

                                                                                                                     ttAA‑TTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCCTGCTTAAGAACCCTTCGCTGACAGCaaaa                        >  2:214661/1‑137 (MQ=255)

                                                                                                                        a‑TTCCGCGGCCAAACCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCTGACAGCAAAATAGAc                   <  8:20954/139‑1 (MQ=255)

                                                                                                                           ttCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCTGACAGCAAAATAGAccc                 >  5:114337/1‑139 (MQ=255)

                                                                                                                               cgacccAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCTGACAGCAAAATAGACCCCCGa             >  5:266379/4‑139 (MQ=255)

                                                                                                                               cgGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCaa                                                                                                   <  2:177705/53‑1 (MQ=255)

                                                                                                                               cgGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCTGACAGCAAAATAGACCCCCGa             >  7:194092/1‑139 (MQ=255)

                                                                                                                               cgGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCTGACAGCAAAATAGACCCCCGa             >  2:10666/1‑139 (MQ=255)

                                                                                                                                     aaTCCAGCCACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCTGACAGCAAAATAGACCCCCGACGTccc       <  3:205381/139‑1 (MQ=255)

                                                                                                                                      aTCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCTGACAGCAAAATAGACCCCCGACGTcccc      <  7:36438/139‑1 (MQ=255)

                                                                                                                                      aTCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCTGACAGCAAAATAGACCCCCGACGTcccc      <  6:289384/139‑1 (MQ=255)

                                                                                                                                      aTCCACCCACGACGCTGCACTCAGCAGCACAGCCATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCTGACAGCAAAATAGACCCCCGACGTcccc      <  6:180548/139‑1 (MQ=255)

                                                                                                                                      aTCCACACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTGGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCTGACAGCAAAATAGACCCCCGACGTcccc      <  6:111284/139‑1 (MQ=255)

GGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGG‑TTAA‑TTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCTGACAGCAAAATAGACCCCCGACGTCCCCAGCG  >  pSIJ663_pHT315_araE_xy/6580‑6854

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`