BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	pSIJ663_pHT315_araE_xy	6,718	A→C	9.4%	intergenic (+1987/–)	ampR → / –	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	pSIJ663_pHT315_araE_xy	6,718	0	A	C	9.4%	2926.2 / 71.7	1403	intergenic (+1987/–)	ampR/–	–/–
Reads supporting (aligned to +/- strand): ref base A (808/434); new base C (26/133); total (836/567)
Fisher's exact test for biased strand distribution p-value = 1.62e-32
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1509 total aligned reads displayed.

GGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGG‑TTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCTGACAGCAAAATAGACCCCCGACGTCCCCAGCGAT  >  pSIJ663_pHT315_araE_xy/6580‑6856

gggATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGG‑TTAATTTCGCGGCCCAATCCAGa                                                                                                                                            >  7:192574/1‑139 (MQ=255)

         gtgggGCGTACGCGCGCCGTAAAGAAACGGCACAAACAAAACTGGCTCGGCACTTTCATCAGCCTTTTGAGCTGCAGCTATAAAAGCTGGGACATTGCTCAGGCCGT‑TTAATTTCGCGGCCCAATCCAGACACGACGCt                                                                                                                                   <  6:143073/139‑1 (MQ=255)

          tgtgGCTTACGGACGCGTTAATGATAAGGCACAAACAAAAATGCCGCGGAAATTGAATCAGCCGTTTTCGCTGCAGATATTAAAGGTGGTAATTTGCAATGGCCGT‑TTAATTAGGCGCCCAAATCCAGACACGACGCt                                                                                                                                   <  4:278369/138‑1 (MQ=255)

          tgtgGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGG‑TTAATTTCGCGGCCCAATCCAGACACGACGCTg                                                                                                                                  >  6:26728/1‑139 (MQ=255)

          tgtgGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGG‑TTAATTTCGCGGCCCAATCCAGACACGACGCTg                                                                                                                                  >  5:112901/1‑139 (MQ=255)

          tgtgGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGG‑TTAATTTCGCGGCCCAATCCAGACACGACGCTg                                                                                                                                  >  7:257747/1‑139 (MQ=255)

            tgGCGTCCGCGCGCCGGAAAGATAAGGAACAACCCAAACTGGCTCGGCACTTTCATCCGCCTGTTGAGCTGCAGCGATTAAAGCGGGGACATTGCTCAGGCCGG‑TTAATTTCGCGGCCCAATCCAGACACGACGCTGCa                                                                                                                                <  8:10545/139‑1 (MQ=255)

            tgGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACCTTGCTCAGTCCGG‑TTAATTTCGCGGCCAAATCCAGACACGACGCTGCa                                                                                                                                >  4:253121/1‑139 (MQ=255)

            tgGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGG‑TTAATTTCGCGGCCCAATCCAGACACGACGCTGCa                                                                                                                                >  1:101726/1‑139 (MQ=255)

            tgGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGG‑TTAATTTCGCGGCCCAATCCAGACACGACGCTGCa                                                                                                                                >  3:89328/1‑139 (MQ=255)

            tgGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGG‑TTAATTTCGCGGCCCAATCCAGACACGACGCTGCa                                                                                                                                >  7:235157/1‑139 (MQ=255)

              gCGTCCGCGCGCCGTAAAGATACGGCATAAACCAACCTGGCTCGGCACTTTCATCCGCCTTTTGAGCGGCAGCGATTAACGCGGGGACATTGCCCCGGCCGT‑TTAATTGCGCGCCCCAAACCACACACGACGCTGCACt                                                                                                                              <  8:36991/139‑1 (MQ=255)

              gCGTACGCGCGCCGGAACGAAACGCCACAAACCAACCTGGCGCGGCACTTACACCAGCCTGTGGCGCGGCAGCTATAAACGCGGGCAAATGGCTCCGCCCGG‑TTAATTTCGCGGCCCAATCCAGACACGACGCTGCACt                                                                                                                              <  4:74386/139‑1 (MQ=255)

               cGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGG‑TTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTc                                                                                                                             >  2:137924/1‑139 (MQ=255)

                gTACGCCCGCCGTAAAGATAAGGCAGAAACCAACCGGCCGCGCAACTTTCATCAGCCTGTTGCGCTGCTGCGATTAACGCTGGGACATTGCTCCGGCGGT‑TTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCa                                                                                                                            <  8:5523/139‑1 (MQ=255)

                 tACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGG‑TTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAg                                                                                                                           >  5:296484/1‑139 (MQ=255)

                 tACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGG‑TTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAg                                                                                                                           >  6:22635/1‑139 (MQ=255)

                 tACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGG‑TTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAg                                                                                                                           >  3:112582/1‑139 (MQ=255)

                 tACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGG‑TTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAg                                                                                                                           >  5:246130/1‑139 (MQ=255)

                 tACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGG‑TTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAg                                                                                                                           >  3:148793/1‑139 (MQ=255)

                 tACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGG‑TTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAg                                                                                                                           >  1:199942/1‑139 (MQ=255)

                        gCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGG‑TTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATcaca                                                                                                                    <  7:219380/139‑1 (MQ=255)

                        gCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGG‑TTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATcaca                                                                                                                    >  1:229941/1‑139 (MQ=255)

                         ccGGAAAGATACGCAACAAACCACTCTGGCTCGGCAATTTCATCAGCATGTTGCGCTCCAGCTATAAACGCGGGGACTTGGCCCAGGCGGG‑TAATTTACGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAg                                                                                                                   <  8:282000/139‑1 (MQ=255)

                         ccGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGG‑TTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATca                                                                                                                      >  5:279085/1‑136 (MQ=255)

                         ccGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGG‑TTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATca                                                                                                                      >  4:102184/1‑136 (MQ=255)

                            gAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGG‑TTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACa                                                                                                                >  7:9118/1‑139 (MQ=255)

                                    aGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGACTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCGGG‑GTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGc                                                                                                        >  1:287039/1‑139 (MQ=255)

                                     ggaataaaCAAAAGTGGCTGGGAATTTTCATCACCTTGTTGAGCTTATGCTTTTAATGCTGGGAAATTGGTAAGGCCGT‑TTATTTTCGCGCCCAAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGcc                                                                                                       <  6:53524/134‑1 (MQ=255)

                                     gccAGAAACCAAACGGGCTCGGCACTCTCCTCCGCCTTTTGCGGTGCAGCGATTAAAGCTGTGACATTGCTCAGGCCTG‑TTAATTTCGCGGCCCAATCCACACACGACGCTGCACTCAGCATCACAGACATTAAATGcc                                                                                                       <  1:96497/137‑1 (MQ=255)

                                          aaaCCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGG‑TTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGCCATTAAATGCCAACGt                                                                                                  >  2:96085/1‑139 (MQ=255)

                                          aaaCCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGG‑TTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGt                                                                                                  >  5:96460/1‑139 (MQ=255)

                                          aaaCCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGG‑TTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGt                                                                                                  >  4:17067/1‑139 (MQ=255)

                                               aaccgggCGCGGCACTTTCAGCAGCCTGTTGAGCTGCAGCTATAAACGCGGGTACATTGCTCCGGCCGG‑TAATTTCCGCGCCCCAAGCCCGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCgg                                                                                             <  8:143525/134‑1 (MQ=255)

                                                aaCTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGG‑TTAATTTCGCGGCCCAATCCAGACACGACGCTGCCCTCAGCATCACAGACATTAAATGCCAACGTTGCgg                                                                                             >  7:279488/1‑138 (MQ=255)

                                                    ggCTCGGCACTTTCAACCGCCTGTTGAGCTGCAGCTATTAAAGGTGGTACATTGCTCAGGCCGG‑TTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAgcg                                                                                        <  6:253648/139‑1 (MQ=255)

                                                     gcccGGCACTTACATCAGCCTGTTGCGCGGCAGCCATAAACGCTGGCACTTTGCTCAGGCCGG‑TAAATTTCGCGGCCCAAACCACCCACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAgcgc                                                                                       <  4:121400/136‑1 (MQ=255)

                                                     gCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGG‑TTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAgcg                                                                                        >  3:3282/1‑138 (MQ=255)

                                                     gCTCGGAAATTACATCCGCGTGTTGAGCTGCAGCTATTAACGCGGGCAATTTCGTCCGGGGGT‑TTAATTTCGCGCCCCAATCCAGCCACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAgcgc                                                                                       <  4:5874/139‑1 (MQ=255)

                                                          gCACTTTCATCCGCCTGTTGAGCGGCAGCGATTAACGCGGGGACATTGCTCAGGCCGG‑TTATTTGCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGc                                                                                  <  1:39404/139‑1 (MQ=255)

                                                            ccTTTCATCTGCCTTTTGTGCGCCCGCTATTAACGCTGTTAAATTGCGCCGGCGGG‑TTAATTGCGCGCCCCACTCCCCACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCaa                                                                                <  3:179823/138‑1 (MQ=255)

                                                            agtttCATCAGCCTGTTGAGCTGCAGCTATAAAAGCGGGTACATTGCTCAGGCGGG‑TAAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCaa                                                                                <  2:167375/137‑1 (MQ=255)

                                                            aCTTTCATCAGCCTGTTGAGCTGCTGCGATTAAAGCGGGTACATTGCTCTGGCCGG‑TTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCaa                                                                                <  8:72384/139‑1 (MQ=255)

                                                            aCTTCCAGCCGCCTGTGGCGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGG‑TAAATTGCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCaa                                                                                <  6:108678/139‑1 (MQ=255)

                                                             cTTTCAGCCGCCTGTGCCGCGGCAGCTATAACAGCTGGGACATTGCTCCGGCCGT‑TAAATTGCGCGCCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCaaa                                                                               <  7:135542/139‑1 (MQ=255)

                                                                taatcaGCGTTTTCTGCTGCAGCTATTAATGCTGGGACTTTGCTCTGGCCGG‑TTATTTCCGCGGCCAAATCCAGCCACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAg                                                                             <  6:99067/136‑1 (MQ=255)

                                                                        cTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGG‑TTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACg                                                                    >  4:141070/1‑139 (MQ=255)

                                                                           ttGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGG‑TTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGgc                                                                  <  7:147559/138‑1 (MQ=255)

                                                                            tGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGG‑TTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATAACAGACATTAAATGCGAACGTTGCGGTAGCGCATTGCAAAAGATATGTACGgcgc                                                                >  5:125010/1‑139 (MQ=255)

                                                                                cTGCAGCGATTAAAGCTGGGCCATTGCTCAGGCCGG‑TTAATTTCGCGGCCCAATCCAGACCCGCCGCTGCACTCTGCATCACAGCCATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTGTc                                                            >  6:168648/1‑139 (MQ=255)

                                                                                cTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGG‑TTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTATATGCCACCGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTc                                                            >  8:239188/1‑139 (MQ=255)

                                                                                cTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGG‑TTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAg                                                                             <  4:94255/122‑1 (MQ=255)

                                                                                cTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGG‑TTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTc                                                            >  4:237351/1‑139 (MQ=255)

                                                                                cTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGG‑TTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTc                                                            >  8:187245/1‑139 (MQ=255)

                                                                                   cAGCGATTAAAGCTGGGACATTGCTCAGGCCGG‑TTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTgg                                                         >  1:55454/1‑139 (MQ=255)

                                                                                   cAGCGATTAAAGCTGGGACATTGCTCAGGCCGG‑TTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTgg                                                         >  1:173602/1‑139 (MQ=255)

                                                                                   cAGCGATTAAAGCTGGGACATTGCTCAGGCCGG‑TTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTgg                                                         >  3:254875/1‑139 (MQ=255)

                                                                                   cAGCGATTAAAGCTGGGACATTGCTCAGGCCGG‑TTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTgg                                                         >  1:147736/1‑139 (MQ=255)

                                                                                     ggttttAAAGCGGGGACATTGCTCCGGCCGG‑TAAATTCCGCGGCCCAATCCAGCCACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGgct                                                       <  6:32554/135‑1 (MQ=255)

                                                                                     gCGATTAACGCTGGGACATGGCTCCGGCCGG‑TTAATTGCGCGGCCCAAGCCACACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGgct                                                       <  2:23523/139‑1 (MQ=255)

                                                                                     gCGATTAAAGCTGGGACATTGCTCAGGCCGG‑TTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCTGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGgct                                                       >  6:20575/1‑139 (MQ=255)

                                                                                      cttttAACGCTGGCACATTGGTAAGGCGGT‑ATTATTTCGCGGCCATATCCACACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGgct                                                       <  1:12363/135‑1 (MQ=255)

                                                                                      ctaTTAAAGGTGGTACTTTGCTCCCGCGGG‑TAAATTTCGGGGCCCATACCCCCCACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGgctt                                                      <  7:179809/137‑1 (MQ=255)

                                                                                            acgcggggACATTGCTCAGGCCGG‑TAAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTaa                                                <  3:123343/134‑1 (MQ=255)

                                                                                              gcggggACATGGCTCAGGCCGG‑TTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTTTGTACGGCGCTTTCTGGCTTGCTTAAGa                                              <  6:125651/136‑1 (MQ=255)

                                                                                                  ggACATTGCTCAGGCCGG‑TTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTCCGGCGCTTTCTGGCTTGCTTAAGAAccc                                          >  2:209366/1‑139 (MQ=255)

                                                                                                  ggACATTGCTCAGGCCGG‑TTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAAccc                                          >  3:135896/1‑139 (MQ=255)

                                                                                                   gACATTGCCCTGGCGGC‑TTATCTTCGCTGCCCAATCCAGCCACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCt                                         <  5:169089/139‑1 (MQ=255)

                                                                                                        tGCTCAGGCCGG‑TTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCt                                    >  6:83513/1‑139 (MQ=255)

                                                                                                        tGCTCAGGCCGG‑TTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCt                                    >  4:205381/1‑139 (MQ=255)

                                                                                                         gcgccggCCGG‑TTATTTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCt                                    <  5:209826/133‑1 (MQ=255)

                                                                                                         gCTCAGGCCGG‑TTAATTTCCCGGCCCAATCCTCACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCt                                    <  5:19104/138‑1 (MQ=255)

                                                                                                          cTCAGGCCGG‑TTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCTGa                                  <  2:42110/139‑1 (MQ=255)

                                                                                                            cAGGCCGG‑TTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCTGa                                  >  1:265490/1‑137 (MQ=255)

                                                                                                             aGGCGGG‑TTAATTTCTCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCTGACAg                               <  8:175481/139‑1 (MQ=255)

                                                                                                               gCCGGTTTAATTTCGGGGCCAATTCCCTACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGGTGCGGTAGCGGATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCTGACAg                               <  8:226457/138‑1 (MQ=255)

                                                                                                               gCCGG‑TTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAATCACCCTTCGCTGACAGc                              >  4:30486/1‑138 (MQ=255)

                                                                                                               gCCGG‑TTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCTGACAg                               <  3:237351/137‑1 (MQ=255)

                                                                                                               gCCGG‑TTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCTGACAGc                              >  3:281109/1‑138 (MQ=255)

                                                                                                               gCCGG‑TTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCTGACAGc                              >  2:62976/1‑138 (MQ=255)

                                                                                                               gCCGG‑TTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCTGACAGc                              >  7:201683/1‑138 (MQ=255)

                                                                                                               gCCGG‑TTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCTGACAGc                              >  5:143238/1‑138 (MQ=255)

                                                                                                               gCCGG‑TTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCTGACAGc                              >  7:162946/1‑138 (MQ=255)

                                                                                                                   g‑TTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATca                                                                                                                      >  5:230944/1‑46 (MQ=255)

                                                                                                                   g‑TTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCTGACAGCaaaa                          >  7:110782/1‑138 (MQ=255)

                                                                                                                     ttAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACTGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCTGACAGCaaaa                          >  8:156984/1‑137 (MQ=255)

                                                                                                                     ttAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCTGACAGCaaaa                          >  5:48671/1‑137 (MQ=255)

                                                                                                                     ttAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCTGACAGCaaaa                          >  7:53729/1‑137 (MQ=255)

                                                                                                                     ttAATTTCGCGGCCCAATCCAGACACGACGATGCACTCAGCATCACCGACATTAAATGCCAACGTTGCGGAAGCGCATGGCAAAAGCTAAGTACTGCGCGTTCTGGCTTGCTTAAGATCCCTTCGCTGACACCAAAAt                         >  6:285674/1‑138 (MQ=255)

                                                                                                                        aTTCCGCGGCCAAACCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCTGACAGCAAAATAGAc                     <  8:20954/139‑1 (MQ=255)

                                                                                                                          ttCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGcc                                                                                                       >  7:33273/1‑55 (MQ=255)

                                                                                                                              cgGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCTGACAGCAAAATAGACCCCCgc               >  1:72969/1‑138 (MQ=255)

                                                                                                                              cgGCCCAATACAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCTGACAGCAAAATAGACCCCCGa               >  8:263623/1‑139 (MQ=255)

                                                                                                                                    aaTCCAGCCACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCTGACAGCAAAATAGACCCCCGACGTccc         <  3:205381/139‑1 (MQ=255)

                                                                                                                                     aTCCAGCCACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCTGACAGCAAAATAGACCCCCGACGTcccc        <  3:58166/139‑1 (MQ=255)

                                                                                                                                     aTCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCTGACAGCAAAATAGACCCCCGACGTcccc        <  7:69028/139‑1 (MQ=255)

                                                                                                                                           acacGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGgcttg                                                     <  2:27964/88‑1 (MQ=255)

                                                                                                                                           acacGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCTGACAGCAAAATAGACCCCCGACGTCCCCAGCGa   >  5:66614/1‑138 (MQ=255)

                                                                                                                                           acacGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCTGACAGCAAAATAGACCCCCGACGTCCCCAGCGa   >  5:262499/1‑138 (MQ=255)

                                                                                                                                           acacGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCTGACAGCAAAATAGACCCCCGACGTCCCCAGCGAt  <  3:75008/139‑1 (MQ=255)

GGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGG‑TTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCTGACAGCAAAATAGACCCCCGACGTCCCCAGCGAT  >  pSIJ663_pHT315_araE_xy/6580‑6856

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`