BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	pSIJ663_pHT315_araE_xy	8,799	A→C	5.4%	intergenic (+4068/–)	ampR → / –	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	pSIJ663_pHT315_araE_xy	8,799	0	A	C	5.4%	4452.9 / 30.9	1556	intergenic (+4068/–)	ampR/–	–/–
Reads supporting (aligned to +/- strand): ref base A (724/703); new base C (14/104); total (738/818)
Fisher's exact test for biased strand distribution p-value = 1.20e-17
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1599 total aligned reads displayed.

AAACTGTTTCAGGAAGCCATAGACCGTCGCGGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTAACCTTCACGACCGCCCCACAGGACATAGTTTTCACCGCCCAATTTATGGGTTGCTTCCATCGCTGTAACAACTTGCGTTGCCGC  >  pSIJ663_pHT315_araE_xy/8662‑8937

aaaCTGTTTCAGGAAGCCATAGACCGTCGCGGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAg                                                                                                                                           <  3:165640/139‑1 (MQ=255)

aaaCTGTTTCAGGAAGCCATAGACCGTCGCGGAATCGTAATCATATTGATGTTTGGGCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAg                                                                                                                                           <  5:216603/139‑1 (MQ=255)

   cTGTTTCAGGAAGCCATAGACCGTCGCGGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCgg                                                                                                                                        <  1:225915/139‑1 (MQ=255)

      tttCAGTAAGCCATAGAACGTCGCGGCATCGTATTCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCTTCTGCATAAAGCGGcc                                                                                                                                      <  4:139718/138‑1 (MQ=255)

      tttCAGGAAGCCATAGACCGTCGCGGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGcc                                                                                                                                      <  1:12007/138‑1 (MQ=255)

        tCAGGAACCCATAGACCGTCGCGGGCTCGGAATCAAATTGATGTTTGGTCGGTTCTTGCGGTTTGGGTTCGATAAGCAACGTGGCGTGGAAACCGATATTATGTTTATGCTCAACCACCCTCAGCATAAAGCGGCCCAg                                                                                                                                   >  4:26779/1‑139 (MQ=255)

            gAAGCCATAGACCGTCGCGGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAgttgt                                                                                                                               >  6:158425/1‑139 (MQ=255)

               gCCATAGACCGTCGCGGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCa                                                                                                                            <  3:55431/139‑1 (MQ=255)

               gCCATAGACCGTCGCGGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCa                                                                                                                            <  6:144755/139‑1 (MQ=255)

               gCCATAGACCGTCGCGGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCa                                                                                                                            <  2:154904/139‑1 (MQ=255)

               gCCATAGACCGTCGCGGCATCGTAATCATATTGAGGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCa                                                                                                                            <  7:126075/139‑1 (MQ=255)

                ccATAGACCGTCGCGGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCAc                                                                                                                           >  3:264803/1‑139 (MQ=255)

                     gACCGTCGCGGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTcc                                                                                                                      <  4:250138/139‑1 (MQ=255)

                          tCGCGGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGcc                                                                                                                                      <  6:107425/118‑1 (MQ=255)

                          tCGCGGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGAct                                                                                                                 >  8:247073/1‑138 (MQ=255)

                          tCGCGGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACg                                                                                                                 >  7:170337/1‑139 (MQ=255)

                          tCGCGGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACg                                                                                                                 >  5:110705/1‑139 (MQ=255)

                          tCGCGGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACg                                                                                                                 >  8:41463/1‑139 (MQ=255)

                          tCGCGGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACg                                                                                                                 >  8:223504/1‑139 (MQ=255)

                          tCGCGGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACg                                                                                                                 >  5:240865/1‑139 (MQ=255)

                          tCGCGGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACg                                                                                                                 >  5:262850/1‑139 (MQ=255)

                          tCGCGGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACg                                                                                                                 >  4:79389/1‑139 (MQ=255)

                          tCGCGGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACg                                                                                                                 >  7:55432/1‑139 (MQ=255)

                          tCGCGGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACg                                                                                                                 >  2:243328/1‑139 (MQ=255)

                          tCGCGGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACg                                                                                                                 >  1:152861/1‑139 (MQ=255)

                          tCGCGGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACg                                                                                                                 >  4:235317/1‑139 (MQ=255)

                          tCGCGGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACg                                                                                                                 >  1:63503/1‑139 (MQ=255)

                          tCGCGGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGAGCACTGGAAACCGATTTTATGATTATGCTCAACCACCATCAGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACg                                                                                                                 >  3:279704/1‑139 (MQ=255)

                          tCGCGGCAGCGTAATAATATGGATGTTGGGGCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGGGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACg                                                                                                                 <  4:157612/139‑1 (MQ=255)

                               gCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGt                                                                                                            <  5:64709/139‑1 (MQ=255)

                               gCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGt                                                                                                            <  5:158425/139‑1 (MQ=255)

                               gCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGt                                                                                                            <  3:74883/139‑1 (MQ=255)

                               gCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGt                                                                                                            <  1:100526/139‑1 (MQ=255)

                               gCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGGTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGGTGTTCACGCTCCTGACGCAAGt                                                                                                            <  3:92489/139‑1 (MQ=255)

                               gCATCGTAATCATATTGATGTTTGGGCGGATCTTGCGGGTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGt                                                                                                            <  5:200376/139‑1 (MQ=255)

                                cATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGcc                                                                                                                                      >  6:272326/1‑112 (MQ=255)

                                 aTCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCACCCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCg                                                                                                          <  8:121457/139‑1 (MQ=255)

                                 aTCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCg                                                                                                          <  1:76977/139‑1 (MQ=255)

                                 aTCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCg                                                                                                          <  4:98049/139‑1 (MQ=255)

                                 aTCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCg                                                                                                          <  1:187071/139‑1 (MQ=255)

                                 aTCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCg                                                                                                          <  2:96045/139‑1 (MQ=255)

                                 aTCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCg                                                                                                          <  6:59980/139‑1 (MQ=255)

                                        tCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCa                                                                                                                            <  6:289147/114‑1 (MQ=255)

                                          atatTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTaa                                                                                                  >  8:86306/1‑138 (MQ=255)

                                            cttGATGTTTGGTCGGTTCTTGCGGTTTCGGCTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACa                                                                                                <  4:209183/137‑1 (MQ=255)

                                            atTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTcc                                                                                                                      <  1:237151/116‑1 (MQ=255)

                                             ttGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAACCCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGc                                                                                              >  6:43829/1‑139 (MQ=255)

                                                  gTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTc                                                                                         >  5:130998/1‑139 (MQ=255)

                                                   tttGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCg                                                                                        >  1:97324/1‑139 (MQ=255)

                                                   tttGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCg                                                                                        <  2:195954/139‑1 (MQ=255)

                                                   tgtGGGCGGTGCTTGCGGTTTCGGCTCGATAAGCAACGGGCCCGGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAACGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCg                                                                                        <  6:189864/137‑1 (MQ=255)

                                                    ttGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGc                                                                                              <  4:29409/132‑1 (MQ=255)

                                                     tGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCCCCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGt                                                                                                            <  6:25374/117‑1 (MQ=255)

                                                     tGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAg                                                                                                                                           <  8:64461/86‑1 (MQ=255)

                                                     tGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGt                                                                                                            <  7:159538/117‑1 (MQ=255)

                                                     tGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGt                                                                                       >  3:21621/1‑138 (MQ=255)

                                                      ggTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAATGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTa                                                                                      <  7:71494/138‑1 (MQ=255)

                                                      ggTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTaa                                                                                     >  4:181798/1‑139 (MQ=255)

                                                      ggTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTaa                                                                                     >  6:235864/1‑139 (MQ=255)

                                                      ggTCGGTTCTTGCGGTTTCGGTGCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTa                                                                                      <  7:243437/138‑1 (MQ=255)

                                                      ggTCGGTTCTTGCGGTTGCGGTTCGATAAGCAACGTGCCCTGGAAACGTATTTTATGTTTATGCGCAAAAACCATCTGAATAAAGCGGCCCAGTTGTTCACGCTCATGACGCAAGTCGGTATTTAACAGCGTTTCGTa                                                                                      <  3:241430/138‑1 (MQ=255)

                                                          ggTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTAACCtt                                                                                 >  5:181608/1‑139 (MQ=255)

                                                          ggTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTAACCtt                                                                                 >  1:28571/1‑139 (MQ=255)

                                                            ttCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCa                                                                                                                            >  2:178167/1‑94 (MQ=255)

                                                            ttCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTAACCTTCa                                                                               >  3:141568/1‑139 (MQ=255)

                                                                      ttCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGt                                                                                                            <  7:257262/100‑1 (MQ=255)

                                                                      ttCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTAACCTTCACGACCGcccc                                                                     >  5:47456/1‑139 (MQ=255)

                                                                      ttCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTAACCTTCACGACCGcc                                                                       >  7:92070/1‑137 (MQ=255)

                                                                      ttCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCACAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTAACCTTCACGACCGcccc                                                                     >  8:182436/1‑139 (MQ=255)

                                                                       tCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACg                                                                                                                          >  2:264605/1‑85 (MQ=255)

                                                                         ggTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTACCCTTCACGACCGCCCca                                                                    >  7:102237/1‑137 (MQ=255)

                                                                             cGATAAGCACCGTGCCCTGGAAACCGATTTTATGTTTATCCTCACCCCCCATCTCCATAACGCGCCCCAGTTGTTCACGCGCCTGCCGCAAGTGGGTATTAAACCGCGTTTCGTAACCTTCACGACCGCCCCACAGGAc                                                              <  8:277997/139‑1 (MQ=255)

                                                                              gATAAGCTACGTGCCGTGGAAACCGATTTTTTTTTTATGCTCAACCACCATCTGCATAACGCGGCCCTGTTGTTCACGCTCCTGACGCATGTCGGTATTTAACAGGGTTTCGTAACCTTCACGACCGCCCCACAGGAc                                                              <  2:214947/138‑1 (MQ=255)

                                                                               aTAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTAACCTTCACTACCGCCCCACAGGACa                                                             >  3:196294/1‑138 (MQ=255)

                                                                               aTAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTAACCTTCACGACCGCCCCACAGGACa                                                             >  1:210599/1‑138 (MQ=255)

                                                                                       caggCCCTGGAACCAGATTTTATGTTTATGTTCTACCACCATGTGCATAAAGGGGCCCTGTTGTTCACGCTCCTCACGCAAGTCGGTTTTTAACAGGGTTTCGTAACCTTCACGACCGCCCCACAGGACATAGTTTTCa                                                    <  2:30063/136‑1 (MQ=255)

                                                                                       cGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTAACCTTCACGACCGCCCCACAGGACATAGTTTTCa                                                    >  3:128652/1‑139 (MQ=255)

                                                                                        gTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTAACCTTCACGACCGCCCCACAGGACATAGTTTTCAc                                                   >  4:47954/1‑139 (MQ=255)

                                                                                         tGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTAACCTTCACGACCGCCCCACAGGACATAGTTTTCAcc                                                  >  1:168094/1‑139 (MQ=255)

                                                                                         tGCCCTGGAAACAGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTAACCTTCACGAc                                                                           >  8:199605/1‑114 (MQ=255)

                                                                                            ctggtaaaCCTATTTTATGTTTATGCTCAACCACCATCTGAAAAACGCGGCCCTGTTGTTCACGCTCCTGACGCAAGTGGGTATTTAACCGCTTTTCGTAACCTTCACGACCGCCCCACAGGACATAGTTTTCACCGcc                                               <  7:282536/134‑1 (MQ=255)

                                                                                            ccgtgAACCCGATTTTTTTTTTATGCTCACCCACATTCTCCAAAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACCGCGTTGCGAAACCTCCACGACCGCCCCACAGGACATAGTTTTCACCGcc                                               <  5:291734/135‑1 (MQ=255)

                                                                                            ccTGGAAACCTATTTTATGTTAATGCACACCCACCATCTGCATAACCCGCCCCTGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTAACCTTCACGACCGCCCCACAGGACATAGTTTTCACCGcc                                               <  8:144949/139‑1 (MQ=255)

                                                                                            ccTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTAACCTACACGACCGCCCCACAGGACATAGATTTCACCGcc                                               >  7:201891/1‑139 (MQ=255)

                                                                                              tGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACg                                                                                                                          <  2:127182/62‑1 (MQ=255)

                                                                                                   cccGATTTTATGTTTATGCTCACCCCCCATCTGCATAACGCGGCCCCGTTGTTCCCGCGCCTGCCGCAAGTCGGTATATAACAGCGTTTCGAAACCTTCACGACCGCCCCACAGGACATAGTTTTCACCGCCCAATTTa                                        <  6:212754/138‑1 (MQ=255)

                                                                                                   aCCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCCCGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTAACCTTCACGACCGCCCCACAGCACATAGTTTTCACCGCCCAATTTa                                        >  2:80688/1‑139 (MQ=255)

                                                                                                    ccGATTTTATGTTTATGCTCACCCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTAACCTTCACGACCGCCCCACAGGACATAGTTTTCACCGCCCAATTTAt                                       <  6:122678/139‑1 (MQ=255)

                                                                                                        ttttATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTAACCTTCACGACCGCCCCACAGGACAGAGTTTTCACCGCCCAATTTATGGGt                                   >  3:34768/1‑139 (MQ=255)

                                                                                                        ttttATGTTTATGCGCAACCACCATCTCAATAAAGCGGCCCAGTTGTTCACGCGCCTGACGCAAGTCGGTATTTAACCGCGTTTCGTAACCTTCACGACCGCCCCACAGGACATAGTTTTCACCGCCCAATTTATggg                                    <  5:278219/138‑1 (MQ=255)

                                                                                                          ttatgtttatgCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTAACCTTCACGACCGCCCCACAGGACATAGTTTTCACCGCCCAATTTATGGGtt                                  <  1:2610/138‑1 (MQ=255)

                                                                                                                  atgCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTAACCTTCACGACCGCCCCACAGGACATAGTTTTCACCGCCCAATTTATGGGTTGCTTCCATc                         >  5:90055/1‑139 (MQ=255)

                                                                                                                  atgCTCAACCACCATCTGCATAAAGCGGCCCAGTGGTTCACGCGCCTGACGCAAGGCGGTATTTAACAGCGTTTCGTAACCTTCACGACCGCCCCACAGGACATAGTTTTCACCGCCCAATTTATGGGTTGCTTCCAt                          <  7:223504/138‑1 (MQ=255)

                                                                                                                  atgCACAACCACCTCCTGCATAAAGCGGCCCCGTTTTTCACGCTCCTGACCCAAGTGGGTATTTAACCGCGTTTAGTAACCTTCACGACCGCCCCACAGGACATAGTTTTCACCGCCCAATTTATGGGTTGCTTCCAt                          <  4:113578/138‑1 (MQ=255)

                                                                                                                       cAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTAACCTTCACGACCGCCCCACAGGACATAGTTTTCACCGCCCAATTTATGGGTTGCTTCCATCGCTg                     >  1:40970/1‑138 (MQ=255)

                                                                                                                        aaCCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGt                                                                                                            >  8:16829/1‑50 (MQ=255)

                                                                                                                                   ccATAACGCGGCCCTGTTGTTCCCGCTCCGGCCGCAAGTGGGTATTTAACAGCGTTTCGAACCCTCCACGACCGCCCCACAGGACATAGTTTTCACCGCCCAATTTATGGGTTGCTTCCATCGCTGTAACAACTTGCGt        <  7:19065/138‑1 (MQ=255)

                                                                                                                                     aTAACGCGGCCGAGTTGAACCCGCACCTGACGCAAGTCGTTTTTTAACCGCGTTTCGTCACCTCCACGACCGCCCCACAGGACATAGTTTTCACCGCCCAATTTATGGGTTGCTTCCATCGCTGTAACAACTTGCGTTg      <  2:56013/139‑1 (MQ=255)

                                                                                                                                        aaGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTAACCTTCACGACCGCCCCACAGGACATAGTTTTCACCGCCCAATTTATGGGTTGCTTCCATCg                        >  7:4119/1‑118 (MQ=255)

                                                                                                                                        aaGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTAACCTTCACGACCGCCCCACAGGACATAGTTTTCACCGCCCAATTTATGGGTTGCTTCCATCGCTGTAACAACTTGCGTTgccg   >  7:145127/1‑139 (MQ=255)

AAACTGTTTCAGGAAGCCATAGACCGTCGCGGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTAACCTTCACGACCGCCCCACAGGACATAGTTTTCACCGCCCAATTTATGGGTTGCTTCCATCGCTGTAACAACTTGCGTTGCCGC  >  pSIJ663_pHT315_araE_xy/8662‑8937

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`