| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP006881 | 1,045,011 | A→C | 5.8% | S276S (TCT→TCG) | U712_05135 ← | putative membrane protein yhfA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 1,045,011 | 0 | A | C | 5.8% | 217.0 / 3.4 | 69 | S276S (TCT→TCG) | U712_05135 | putative membrane protein yhfA |
| Reads supporting (aligned to +/- strand): ref base A (31/34); new base C (2/2); total (33/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01 | |||||||||||
AGCGGCAATCGGGATGACGGGGCCGAAAACCTTAAAACCGAACTTAAATCCGTCAATAAAATAGCCGGTGATTTTTTCAAGCCCTTTATGCTTATACACGAAAAAATGAACGGTAAAGAGGATACAAATTGCAGACCCGCCAATTAACGCGGTTGCATCATTTCCTTGTAGGTTAAACAAGAGCATGCAGGCGATATCTGCAAGAAAGGCAAGCGGAATCAAAAAAGCCAGTATGGACCGCAGGCGTTTTGGCAAGTACAGCGAGTTGTC > CP006881/1044879‑1045148 | agCGGCAATCGGGATGACGGGGCCGAAAACCTTAAAACCGAACTTAAATCCGTCAATAAAATAGCCGGTGATTTTTTCAAGCCCTTTATGCTTATACACGAAAAAATGAACGGTAAAGAGGATACAAATTGCAGACCCg > 8:116910/1‑139 (MQ=255)agCGGCAATCGGGATGACGGGGCCGAAAACCTTAAAACCGAACTTAAATCCGTCAATAAAATAGCCGGTGATTTTTTCAAGCCCTTTATGCTTATACACGAAAAAATGAACGGTAAAGAGGATACAAATTGCAGACCCg > 7:236012/1‑139 (MQ=255) gCGGCAATCGGGATGACGGGGCCGAAAACCTTAAAACCGAACTTAAATCCGTCAATAAAATAGCCGGTGATTTTTTCAAGCCCTTTATGCTTATACACGAAAAAATGAACGGTAAAGAGGATACAAATTGCAGACCAGc > 2:202007/1‑139 (MQ=255) tCGGGATGACGGGGCCGAAAACCTTAAAACCGAACTTAAATCCGTCAATAAAATAGCCGGTGATTTTTTCAAGCCCTTTATGCTTATACACGAAAAAATGAACGGTAAAGAGGATACAAATTGCAGACCCGCCAATTaa < 5:265850/139‑1 (MQ=255) tCGGGATGACGGGGCCGAAAACCTTAAAACCGAACTTAAATCCGTCAATAAAATAGCCGGTGATTTTTTCAAGCCCTTTATGCTTATACACGAAAAAATGAACGGTAAAGAGGATACAAATTGCAGACCCGCCAATTaa < 7:98296/139‑1 (MQ=255) gggATGCCGGGGCCGAAAACCTTAAAACCGAACTTAAATCCGTCAATAAAATAGCCGGTGATTTTTTCAAGCCCTTTATGCTTATACACGAAAAAATGAACGGTAAAGAGGATACAAATTGCAGACCCGCCAATTAAcg < 2:229713/139‑1 (MQ=255) gggATGACGGGGCCGAAAACCTTAAAACCGAACTTAAATCCGTCAATAAAATAGCCGGTGATTTTTTCAAGCCCTTTATGCTTATACACGAAAAAATGAACGGTAAAGAGGATACAAATTGCAGACCCGCCAATTAAcg < 2:69283/139‑1 (MQ=255) aCGGGGCCGAAAACCTTAAAACCGAACTTAAATCCGTCAATAAAATAGCCGGTGATTTTTTCAAGCCCTTTATGCTTATACACGAAAAAATGAACGGTAAAGAGGATACAAATTGCAGACCCGCCAATTAACGCGGTTg > 3:10783/1‑139 (MQ=255) ccGAAAACCTTAAAACCGAACTTAAATCCGTCAATAAAATAGCCGGTGATTTTTTCAAGCCCTTTATGCTTATACACGAAAAAATGAACGGTAAAGAGGATACAAATTGCAGACCCGCCAATTAAcg < 1:15030/127‑1 (MQ=255) ccGAAAACCTTAAAACCGAACTTAAATCCGTCAATAAAATAGCCGGTGATTTTTTCAAGCCCTTTATGCTTATACACGAAAAAATGAACGGTAAAGAGGATACAAATTGCAGACCCGCCAATTAAcg > 2:15030/1‑127 (MQ=255) cGAAAACCTTAAAACCGAACTTAAATCCGTCAAAAAAATACCCGGTGATTTTTTCAAGCCCTTTATGCTTATACACGAAAAAATGAACGGTAAAGAGGATACAAATTGCAGACCCGCCAATTAACGCGGTTGCATCAtt < 8:243004/139‑1 (MQ=255) cGAAAACCTTAAAACCGAACTTAAATCCGTAAATAAAATAGCCTGTGATTTTTTCAAGCCCTATATGATTATAAACGAAAAACTGAACGGTAAAGAGGATACAAATTGCAGACCCGCCAATTAACGCGGTTGCATCAtt < 3:78033/139‑1 (MQ=255) aCCTTAAAACCGAACTTAAATCCGTCAATAAAATAGCCGGTGATTTTTTCAAGCCCTTTATGCTTATACACGAAAAAATGAACGGTAAAGAGGATACAAATTGCAGACCCGCCAATTAACGCGGTTGCATCATTTCCtt < 4:101823/139‑1 (MQ=255) ccTTAAAACCGAACTTAAATCCGTCAATAAAATAGCCGGTGATTTTTTCAAGCCCTTTATGCTTATACACGAAAAAATGAACGGTAAAGAGGATACAAATTGCAGACCCGCCAATTAACGCGGTTGCATCATTTCCTTg < 5:218260/139‑1 (MQ=255) aaaCCGAACTTAAATCCGTCAATAAAATAGCCGGTGATTTTTTCAAGCCCTTTATGCTTATACACGAAAAAATGAACGGTAAAGAGGATACAAATTGCAGACCCGCCAATTAACGCGGTTGca > 2:49129/1‑123 (MQ=255) aaaCCGAACTTAAATCCGTCAATAAAATAGCCGGTGATTTTTTCAAGCCCTTTATGCTTATACACGAAAAAATGAACGGTAAAGAGGATACAAATTGCAGACCCGCCAATTAACGCGGTTGca < 1:49129/123‑1 (MQ=255) aaaCCGAACTTAAATCCGTCAATAAAATAGCCGGTGATTTTTTCAAGCCCTTTATGCTTATACACGAAAAAATGAACGGTAAAGAGGATACAAATTGCAGACCCGCCAATTAACGCGGTTGCATCATTTCCTTGTAgg < 3:138301/138‑1 (MQ=255) aaCTTAAATCCGTCAATAAAATAGCCGGTGATTTTTTCAAGCCCTTTATGCTTATACACGAAAAAATGAACGGTAAAGAGGATACAAATTGCAGACCCGCCAATTAACGCGGTTGCATCATTTCCTTGTAGGTTAAAc > 3:138314/1‑138 (MQ=255) aaCTTAAATCCGTCAATAAAATAGCCGGTGATTTTTTCAAGCCCTTTATGCTTATACACGAAAAAATGAACGGTAAAGAGGATACAAATTGCAGACCCGCCAATTAACGCGGTTGCATCATTTCCTTGTAGGTTAAAc > 3:142529/1‑138 (MQ=255) aCTTAAATCCGTCAATAAAATAGCCGGTGATTTTTTCAAGCCCTTTATGCTTATACACGAAAAAATGAACGGTAAAGAGGATACAAATTGCAGACCCGCCAATTAACGCGGTTGCATCATTTCCTTGTAGGTTAAACaa < 2:37456/139‑1 (MQ=255) cTTAAATCCGTCAATAAAATAGCCGGTGATTTTTTCAAGCCCTTTATGCTTATACACGAAAAAATGAACGGTAAAGAGGATACAAATTGCAGACCCGCCAATTAACGCGGTTGCATCATTTCCTTGTAGGTTAAACAag < 4:10783/139‑1 (MQ=255) tCAATAAAATAGCCGGTGATTTTTTCAAGCCCTTTATGCTTATACACGAAAAAATGAACGGTAAAGAGGATACAAATTGCCGACCCGCCAATTAACGCGGTTGCATCATTTCCtt > 4:105701/1‑115 (MQ=255) tCAATAAAATAGCCGGTGATTTTTTCAAGCCCTTTATGCTTATACACGAAAAAATGAACGGTAAAGAGGATACAAATTGCCGACCCGCCAATTAACGCGGTTGCATCATTTCCtt > 4:101429/1‑115 (MQ=255) tCAATAAAATAGCCGGTGATTTTTTCAAGCCCTTTATGCTTATACACGAAAAAATGAACGGTAAAGAGGATACAAATTGCCGACCCGCCAATTAACGCGGTTGCATCATTTCCtt < 3:101429/115‑1 (MQ=255) tCAATAAAATAGCCGGTGATTTTTTCAAGCCCTTTATGCTTATACACGAAAAAATGAACGGTAAAGAGGATACAAATTGCCGACCCGCCAATTAACGCGGTTGCATCATTTCCtt < 3:105701/115‑1 (MQ=255) tCAATAAAATAGCCGGTGATTTTTTCAAGCCCTTTATGCTTATACACGAAAAAATGAACGGTAAAGAGGATACAAATTGCAGACCCGCCAATTAACGCGGTTGCATCATTTCCTTGTAgg < 4:45777/120‑1 (MQ=255) tCAATAAAATAGCCGGTGATTTTTTCAAGCCCTTTATGCTTATACACGAAAAAATGAACGGTAAAGAGGATACAAATTGCAGACCCGCCAATTAACGCGGTTGCATCATTTCCTTGTAgg > 3:45777/1‑120 (MQ=255) tCAATAAAATAGCCGGTGATTTTTTCAAGCCCTTTATGCTTATACACGAAAAAATGAACGGTAAAGAGGATACAAATTGCAGACCCGCCAATTAACGCGGTTGCATCATTTCCTTGTAGGTTAAACAAGAGCATGCAgg > 7:215398/1‑139 (MQ=255) aaTAAAATAGCCGGTGATTTTTTCAAGCCCTTTATGCTTATACACGAAAAAATGAACGGTAAAGAGGATACAAATTGCAGACCCGCCAATTAACGCGGTTGCATCATTTCCTTGTAGGTTAAACAAGAGCATGCAGGCg < 6:82103/139‑1 (MQ=255) tAAAATAGCCGGTGATTTTTTCAAGCCCTTTATGCTTATACACGAAAAAATGAACGGTAAAGAGGATACAAATTACAGACCCGCCAATTAACGCGGTTGCATCATTTCCTTGTAGGTTAAACAAGAGCATGCAGGCGat < 8:99913/139‑1 (MQ=255) gCCGGTGATTTTTTCAAGCCCTTTATGCTTATACACGAAAAAATGAACGGTAAAGAGGATACAAATTGCAGACCCGCCAATTAACGCGGTTGCATCATTTCCTTGTAGGTTAAACAAGAGCATGCAGGCGATATCTGCa < 7:255057/139‑1 (MQ=255) ccGGTGATTTTTTCAAGCCCTTTATGCTTATACACGAAAAAATGAACGGTAAAGAGGATACAAATTGCAGACCCGCCAATTAACGCGGTTGCATCATTTCCTTGTAGGTTAAACAAGAGCATGCAGGCGATATCTGc > 8:255057/1‑137 (MQ=255) tttCAAGCCCTTTATGCTTATACACGAAAAAATGAACGGTAAAGAGGATACAAATTGCAGACCCGCCAATTAACGCGGTTGCATCATTTCCTTGTAGGTTAAACAAGAGCATGCAGGCGATATCTGCAAGAAAGGCAAg > 1:80553/1‑139 (MQ=255) tATGCTTATACACGAAAAAATGAACGGTAAAGAGGATACAAATTGCAGACCCGCCAATTAACGCGGTTGCATCATTTCCTTGTAGGTTAAACAAGAGCATGCAGGCGATATCTGCAAGAAAGGCAAGCGGAATCaaaa > 2:164806/1‑138 (MQ=255) tATGCTTATACACGAAAAAATGAACGGTAAAGAGGATACAAATTGCAGACCCGCCAATTAACGCGGTTGCATCATTTCCTTGTAGGTTAAACAAGAGCATGCAGGCGATATCTGCAAGAAAGGCAAGCGGAATCaaa < 7:116910/137‑1 (MQ=255) tATGCTTATACACGAAAAAATGAACGGTAAAGAGGATACAAATTGCAGACCCGCCAATTAACGCGGTTGCATCATTTCCTTGTAGGTTAAACAAGAGCATGCAGGCGATATCTGCAAGAAAGGCAAGCGGAATCaaa < 4:138314/137‑1 (MQ=255) cTTATACACGAAAAAATGAACGGTAAAGAGGATACAAATTGCAGACCCGCCAATTAACGCGGTTGCATCATTTCCTTGTAGGTTAAACAAGAGCATGCAGGCGATATCTGCAAGAAAGGCAAGCGGAATCAAAAAAGc < 6:125067/138‑1 (MQ=255) cTTATACACGAAAAAATGAACGGTAAAGAGGATACAAATTGCAGACCCGCCAATTAACGCGGTTGCATCATTTCCTTGTAGGTTAAACAAGAGCATGCAGGCGATATCTGCAAGAAAGGCAAGCGGAATCAAAAAAGc < 4:132338/138‑1 (MQ=255) tataCACGAAAAAATGAACGGTAAAGAGGATACAAATTGCAGACCCGCCAATTAACGCGGTTGCATCATTTCCTTGTAGGTTAAACAAGAGCATGCAGGCGATATCTGCAAGAAAGGCAAGCGGAATCAAAAAAGCCAg > 2:15110/1‑139 (MQ=255) tataCACGAAAAAATGAACGGTAAAGAGGATACAAATTGCAGACCCGCCAATTAACGCGGTTGCATCATTTCCTTGTAGGTTAAACAAGAGCATGCAGGCGATATCTGCAAGAAAGGCAAGCGGAATCAAAAAAGCCAg > 5:186070/1‑139 (MQ=255) tataCACGAAAAAATGAACGGTAAAGAGGATACAAATTGCAGACCCGCCAATTAACGCGGTTGCATCATTTCCTTGTAGGTTAAACAAGAGCATGCAGGCGATATCTGCAAGAAAGGCAAGCGGAATCAAAAAAGCCAg > 6:223647/1‑139 (MQ=255) taCACGAAAAAATGAACGGTAAAGAGGATACAAATTGCAGACCCGCCAATTAACGCGGTTGCATCATTTCCTTGTAGGTTAAACAAGAGCATGCAGGCGATATCTGCAAGAAAGGCAAGCGGAATCAAAAAAGCCAGTa > 8:76615/1‑139 (MQ=255) taCACGAAAAAATGAACGGTAAAGAGGATACAAATTGCAGACCCGCCAATTAACGCGGTTGCATCATTTCCTTGTAGGTTAAACAAGAGCATGCAGGCGATATCTGCAAGAAAGGCAAGCGGAATCAAAAAAGCCAGTa > 7:109131/1‑139 (MQ=255) taCACGAAAAAATGAACGGTAAAGAGGATACAAATTGCAGACCCGCCAATTAACGCGGTTGCATCATTTCCTTGTAGGTTAAACAAGAGCATGCAGGCGATATCTGCAAGAAAGGCAAGCGGAATCAAAAAAGCCAGTa > 8:253909/1‑139 (MQ=255) gAAAAAATGAACGGTAAAGAGGATACAAATTGCAGACCCGCCAATTAACGCGGTTGCATCATTTCCTTGTAGGTTAAACAAGAGCATGCAGGCGATATCTGCAAGAAAGGCAAGCGGAATCAAAAAAg < 1:124067/128‑1 (MQ=255) gAAAAAATGAACGGTAAAGAGGATACAAATTGCAGACCCGCCAATTAACGCGGTTGCATCATTTCCTTGTAGGTTAAACAAGAGCATGCAGGCGATATCTGCAAGAAAGGCAAGCGGAATCAAAAAAg > 2:124067/1‑128 (MQ=255) gAAAAAATGAACGGTAAAGAGGATACAAATTGCAGACCCGCCAATTAACGCGGTTGCATCATTTCCTTGTAGGTTAAACAAGAGCATGCAGGCGATATCTGCAAGAAAGGCAAGCGGAATCAAAAAAGCCAGTATGGAc < 6:186070/139‑1 (MQ=255) aCGGTAAAGAGGATACAAATTGCAGACCCGCCAATTAACGCGGTTGCATCATTTCCTTGTAGGTTAAACAAGAGCATGCAGGCGATATCTGCAAGAAAGGCAAGCGGAATCAAAAAAGCCAGTATGGACCGCAGGCGtt > 1:283607/1‑139 (MQ=255) tAAAGAGGATACAAATTGCAGACCCGCCAATTAACGCGGTTGCATCATTTCCTTGTAGGTTAAACAAGAGCATGCAGGCGATATCTGCAAGAAAGGCAAGCGGAATCAAAAAAGCCAGTATGGACCGCAGGCGTTTTgg < 1:156586/139‑1 (MQ=255) aaaGAGGATACAAATTGAAGACCCTCCAATAAACGCGGTTGCATCATTTCCTTGTAGGTTAAACAAGAGCATGCAGGCGATATCTGCAAGAAAGGCAAGCGGAATCAAAAAAGCCAGTATGGACCGCAGGCGTTTTGGc < 1:202007/139‑1 (MQ=255) aaGAGGATACAAATTGCAGACCCGCCAATTAACGCGGTTGCATCATTTCCTTGTAGGTTAAACAAGAGCATGCAGGCGATATCTGCAAGAAAGGCAAGCGGAATCAAAAAAGCCAGTATGGACCGCAGGCGTTTTGGCa < 2:242994/139‑1 (MQ=255) agGATACAAATTGCAGACCCGCCAATTAACGCGGTTGCATCATTTCCTTGTAGGTTAAACAAGAGCATGCAGGCGATATCTGCAAGAAAGGCAAGCGGAATCAAAAAAGCCAGTATGGACCGCAGGCGTTTTGGCAAg > 4:66209/1‑138 (MQ=255) aTACAAATTGCAGACCCGCCAATTAACGCGGTTGCATCATTTCCTTGTAGGTTAAACAAGAGCATGCAGGCGATATCTGCAAGAAAGGCAAGCGGAATCAAAAAAGCCAGTATGGACCGCAGGCGTTTTGGCAAGTACa < 8:215398/139‑1 (MQ=255) aCAAATTGCAGACCCGCCAATTAACGCGGTTGCATCATTTCCTTGTAgg < 7:110519/49‑1 (MQ=255) aCAAATTGCAGACCCGCCAATTAACGCGGTTGCATCATTTCCTTGTAgg > 8:110519/1‑49 (MQ=255) aCAAATTGCAGACCCGCCAATTAACGCGGTTGCATCATTTCCTTGTAGGTTAAACAAGAGCATg > 6:154778/1‑64 (MQ=255) aCAAATTGCAGACCCGCCAATTAACGCGGTTGCATCATTTCCTTGTAGGTTAAACAAGAGCATg < 5:154778/64‑1 (MQ=255) aCAAATGGCAGACCCGCCAATTAACGCGGTTGCATCATTTCCTTGTAGGTTAAACAAGAGCATGCAGGCGATATCTGCAAGAAAGGCAAGCGGAATCAAAAAAGCCAGTATGGACCGCAGGCGTTTTGGCAAGTACAGc < 2:276545/139‑1 (MQ=255) aaTTGCAGACCCGCCAATTAACGCGGTTGCATCATTTCCTTGTAGGTTAAACAAGAGCATGCAGGCGATATCTGCAAGAAAGGCAAGCGGAATCAAAAAAGCCAGTATGGACCGCAGGCGTTTTGGCAAGTACAGCGAg < 4:142529/139‑1 (MQ=255) aTTGCAGACCCGCCAATTAACGCGGTTGCATCATTTCCTTGTAGGTTAAACAAGAGCATGCAGGCGATATCTGCAAGAAAGGCAAGCTGAATCAAAAAAGCCAGTATGGACCGCAGGCGTTTTGGCAAGTACAGCGAGt > 6:290458/1‑139 (MQ=255) gCAGACCCGCCAATTAACGCGGTTGCATCATTTCCTTGTAGGTTa > 6:245062/1‑45 (MQ=255) gCAGACCCGCCAATTAACGCGGTTGCATCATTTCCTTGTAGGTTa < 5:245062/45‑1 (MQ=255) gCAGACCCGCCAATTAACGCGGTTGCATCATTTCCTTGTAGGTTAAACAAGAGCATGCAGGCGATATCTGCAAGAAAGGCATGCGGAATCAAAAAAGCCAGTATGGACCGCAGGCGTTTTGGCAAGTACAGCGAGTTGt > 4:177971/1‑139 (MQ=255) gCAGACCCGCCAATTAACGCGGTTGCATCATTTCCTTGTAGGTTAAACAAGAGCATGCAGGCGATATCTGCAAGAAAGGCAAGCGGAATCAAAAAAg < 2:139281/97‑1 (MQ=255) gCAGACCCGCCAATTAACGCGGTTGCATCATTTCCTTGTAGGTTAAACAAGAGCATGCAGGCGATATCTGCAAGAAAGGCAAGCGGAATCAAAAAAg > 1:139281/1‑97 (MQ=255) cAGACCCGCCAATTAACGCGGTTGCATCATTTCCTTGTAGGTTAAACAagag < 7:272488/52‑1 (MQ=255) cAGACCCGCCAATTAACGCGGTTGCATCATTTCCTTGTAGGTTAAACAagag > 8:272488/1‑52 (MQ=255) cAGACCCGCCAATTAACGCGGTTGCATCATTTCCTTGTAGGTTAAACAAGAGCATGCAGGCGATATCTGCAAGAAAGGCAAGCGGAATCAAAAAAGCCAGTATGGACCGCAGGCGTTTTGGCAAGTACAGCGAGTTGTc > 4:131639/1‑139 (MQ=255) cAGACCCGCCAATTAACGCGGTTGCATCATTTCCTTGTAGGTTAAACAAGAGCATGCAGGCGATATCTGCAAGAAAGGCAAGCGGAATCAAAAAAGCCAGTATGGACCGCAGGCGTTTTGGCAAGTACAGCGAGTTGTc > 8:48034/1‑139 (MQ=255) | AGCGGCAATCGGGATGACGGGGCCGAAAACCTTAAAACCGAACTTAAATCCGTCAATAAAATAGCCGGTGATTTTTTCAAGCCCTTTATGCTTATACACGAAAAAATGAACGGTAAAGAGGATACAAATTGCAGACCCGCCAATTAACGCGGTTGCATCATTTCCTTGTAGGTTAAACAAGAGCATGCAGGCGATATCTGCAAGAAAGGCAAGCGGAATCAAAAAAGCCAGTATGGACCGCAGGCGTTTTGGCAAGTACAGCGAGTTGTC > CP006881/1044879‑1045148 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |