Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP006881	1,371,810	C→T	6.0%	M269I (ATG→ATA)	U712_07010 ←	Signaling protein ykoW

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	1,371,810	0	C	T	6.0%	208.1 / 2.6	68	M269I (ATG→ATA)	U712_07010	Signaling protein ykoW
Reads supporting (aligned to +/- strand):  ref base C (32/29);  new base T (2/2);  total (37/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.55e-01

CTTCGTCATGTCTTTACAAATGACATAAATGCTGTCTAGTTCCTGATTGACCTCAACAGGTATAAGCGTAATATTTAAGTGAAACTGGCCTTTTTCAGTGATAAGATCGCTATCGCTGTTCATCGCTTGTTTATCTTTTTTAACTTGCTCAAAAAGGCGCTTAACCTTCAGTGATGTGTAATTCCTCCAGTCATGCATGCTGAAGCCAAATTTGCTGATCATTTCCTCACCAGCGTGATTGGAAGATATTATGTCTC  >  CP006881/1371690‑1371946                                                                                                                         |                                                                                                                                         cTTCGTCATGTCTTTACAAATGACATAAATGCTGTCTAGTTCCTGATTGACCTCAACAGGTATAAGCGTAATATTTAAGTGAAACTGGCCTTTTTCAGTGATAAGATCGCTATCGCTGTTCATCGCTTGTTTATCtttt                                                                                                                        >  6:56192/1‑139 (MQ=255) cTTCGTCATGTCTTTACAAATGACATAAATGCTGTCTAGTTCCTGATTGACCTCAACAGGTATAAGCGTAATATTTAAGTGAAACTGGCCTTTTTCAGTGATAAGATCGCTATCGCTGTTCATCGCTTGTTTATCtttt                                                                                                                        >  6:216265/1‑139 (MQ=255) cTTCGTCATGTCTTTACAAATGACATAAATGCTGTCTAGTTCCTGATTGACCTCAACAGGTATAAGCGTAATATTTAAGTGAAACTGGCCTTTTTCAGTGATAAGATCGCTATCGCTGTTCATCGCTTGTTTATCtttt                                                                                                                        >  5:262632/1‑139 (MQ=255) cTTCGTCATGTCTTTACAAATGACATAAATGCTGTCTAGTTCCTGATTGACCTCAACAGGTATAAGCGTAATATTTAAGTGAAACTGGCCTTTTTCAGTGATAAGATCGCTATCGCTGTTAATCGCTTGTTTATCtttt                                                                                                                        >  2:101670/1‑139 (MQ=255)      tCATGTCTTTACAAATGACATAAATGCTGTCTAGTTCCTGATTGACCTCAACAGGTATAAGCGTAATATTTAAGTGAAACTGGCCTTTTTCAGTGATAAGATCGCTATCGCTGTTCATCGCTTGTTTATCTTTTTTAAc                                                                                                                   >  7:7022/1‑139 (MQ=255)      tCATGTCTTTACAAATGACATAAATGCTGTCTAGTTCCTGATTGACCTCAACAGGTATAAGCGTAATATTTAAGTGAAACTGGCCTTTTTCAGTGATAAGATCGCTATCGCTGTTCATCGCTTGTTTATCTTTTTTAAc                                                                                                                   >  4:157022/1‑139 (MQ=255)      tCATGTCTTTACAAATGACATAAATGCTGTCTAGTTCCTGATTGACCTCAACAGGTATAAGCGTAATATTTAAGTGAAACTGGCCTTTTTCAGTGATAAGATCGCTATCGCTGTTCATCGCTTGTTTATCTTTTTTAAc                                                                                                                   >  4:229612/1‑139 (MQ=255)                aCAAATGACATAAATGCTGTCTAGTTCCTGATTGACCTCAACAGGTATAAGCGTAATATTTAAGTGAAACTGGCCTTTTTCAGTGATAAGATCGCTATCGCTGTTCATCGCTTGTTTAt                                                                                                                             <  8:163772/119‑1 (MQ=255)                aCAAATGACATAAATGCTGTCTAGTTCCTGATTGACCTCAACAGGTATAAGCGTAATATTTAAGTGAAACTGGCCTTTTTCAGTGATAAGATCGCTATCGCTGTTCATCGCTTGTTTAt                                                                                                                             >  7:163772/1‑119 (MQ=255)                       aCATAAATGCTGTCTAGTTCCTGATTGACCTCAACAGGTATAAGCGTAATATTTAAGTGAAACTGGCCTTTTTCAGTGATAAGATCGCTATCGCTGTTCATCGCTTGTTTATCTTTTTTAACTTGCTCAAAAAGGCGCt                                                                                                  <  7:48812/139‑1 (MQ=255)                       aCATAAATGCTGTCTAGTTCCTGATTGACCTCAACAGGTATAAGCGTAATATTTAAGTGAAACTGGCCTTTTTCAGTGATAAGATCGCTATCGCTGTTCATCGCTTGTTTATCTTTTTTAACTTGCTCAAAAAGGCGCt                                                                                                  <  2:266151/139‑1 (MQ=255)                            aaTGCTGTCTAGTTCCTGATTGACCTCAACAGGTATAAGCGTAATATTTAAGTGAAACTGGCCTTTTTCAGTGATAAGATCGCTATCGCAGTTCATCGCTTGTTTATCTTTTTTAACTTGCTCAAAAAGGCGCTTAAcc                                                                                             >  4:64983/1‑139 (MQ=255)                                cTGTCTAGTTCCTGATTGACCTCAACAGGTATAAGCGTAATATTTAAGTGAAACTGGCCTTTTTCAGTGATAAGATCGCTATCGCTGTTCATCGCTTGTTTATCTTTTTTAACTTGCTCAAAAAGGCGCt                                                                                                  >  1:139719/1‑130 (MQ=255)                                cTGTCTAGTTCCTGATTGACCTCAACAGGTATAAGCGTAATATTTAAGTGAAACTGGCCTTTTTCAGTGATAAGATCGCTATCGCTGTTCATCGCTTGTTTATCTTTTTTAACTTGCTCAAAAAGGCGCt                                                                                                  <  2:139719/130‑1 (MQ=255)                                cTGTCTAGTTCCTGATTGACCTCAACAGGTATAAGCGTAATATTTAAGTGAAACTGGCCTTTTTCAGTGATAAGATCGCTATCGCTGTTCATCGCTTGTTTATCTTTTTTAACTTGCTCAAAAAGGCGCTTAACCtt                                                                                           >  2:91187/1‑137 (MQ=255)                                   tCTAGTTCCTGATTGACCTCAACAGGTATAAGCGTAATATTTAAGTGAAACTGGCCTTTTTCAGTGATAAGATCGCTATCGCTGTTCATCGCTTGTTTATCTTTTTTAACTTGCTCAAAAAGGCGCTTAACCTTCAg                                                                                        <  1:91187/137‑1 (MQ=255)                                   tCTAGTTCCTGATTGACCTCAACAGGTATAAGCGTAATATTTAAGTGAAACTGGCCTTTTTCAGTGATAAGATCGCTATCGCTGTTCATCGCTTGTTTATCTTTTTTAACTTGCTCAAAAAGGCGCTTAACCTTCAGTg                                                                                      <  4:88428/139‑1 (MQ=255)                                   tCTAGTTCCTGATTGACCTCAACAGGTATAAGCGTAATATTTAAGTGAAACTGGCCTTTTTCAGTGATAAGATCGCTATCGCTGTTCATCGCTTGTTTATCTTTTTTAACTTGCTCAAAAAGGCGCTTAACCTTCAGTg                                                                                      <  6:262632/139‑1 (MQ=255)                                   tCTAGTTCCTGATTGACCTCAACAGGTATAAGCGTAATATTTAAGTGAAACTGGCCTTTTTCAGTGATAAGATCGCTATCGCTGTTCATCGCTTGTTTATCTTTTTTAACTTGCTCAAAAAGGCGCTTAACCTTCAGTg                                                                                      <  6:7230/139‑1 (MQ=255)                                   tCTAGTTCCTGATTGACCTCAACAGGTATAAGCGTAATATTTAAGTGAAACTGGCCTTTTTCAGTGATAAGATCGCTATCGCTGTTCATCGCTTGTTTATCTTTTTTAACTTGCTCAAAAAGGCGCTTAACCTTCAGTg                                                                                      <  3:157022/139‑1 (MQ=255)                                   tCTAGTTCCTGATTGACCTCAACAGGTATAAGCGTAATATTTAAGTGAAACTGGCCTTTTTCAGTGATAAGATCGCTATCGCTGTTCATCGCTTGTTTATCTTTTTTAACTTGCTCAAAAAGGCGCTTAACCTTCAGTg                                                                                      <  1:101670/139‑1 (MQ=255)                                   tCTAGTTCCTGATTGACCTCAACAGGTATAAGCGTAATATTTAAGAGAAACTGGCCTTTTTCAGTGATAAGATCGCTATCGCTGTTCATCGCTTGTTTATCTTTTTTAACTTGCTCAAAAAGGCGCTTAACCTTCAGt                                                                                       >  4:93169/1‑138 (MQ=255)                                       gTTCCTGATTGACCTCAACAGGTATAAGCGTAATATTTAAGTGAAACTGGCCTTTTTCAGTGATAAGATCGCTATCGCTGTTCATCGCTTGTTTATCTTTTTTAACTTGCTCAAAAAGGCGCTTAACCTTCAGTGAtg                                                                                   <  8:7022/138‑1 (MQ=255)                                        ttCCTGATTGACATCAAAAGGAATAAGATTAATATTTAAGTGAAACTGGCCTTTTTCAGTCATAAGATCGCTATCTATGTTAATATCTTGTTTATCTTTTTTAACTAGCTCATAAAGGAGATTAACCTTCAGTGAtgtg                                                                                 >  7:290108/1‑139 (MQ=255)                                             gATTGACCTCAACAGGTATAAGCGTAATATTTAAGTGAAACTGGCCTTTTTCAGTGATAAGATCGCTATCGCTGTTCATCGCTTGTTTATCTTTTTTAACTTGCTCAAAAAGGCGCTTAACCTTCAGTGATGTGTAAtt                                                                            >  3:47258/1‑139 (MQ=255)                                             gATTGACCTCAACAGGTATAAGCGTAATATTTAAGTGAAACTGGCCTTTTTCAGTGATAAGATCGCTATCGCTGTTCATCGCTTGTTTATCTTTTTTAACTTGCTCAAAAAGGCGCTTAACCTTCAGTGATGTGTAAtt                                                                            >  8:139226/1‑139 (MQ=255)                                             gATTGACCTCAACAGGTATAAGCGTAATATTTAAGTGAAACTGGCCTTTTTCAGTGATAAGATCGCTATCGCTGTTCATCGCTTGTTTATCTTTTTTAACTTGCTCAAAAAGGCGCTTAACATTCAGTGATGTGTAAt                                                                             >  1:36472/1‑138 (MQ=255)                                                            gTATAAGCGTAATATTTAAGTGAAACTGGCCTTTTTCAGTGATAAGATCGCTATCGCTGTTCATCGCTTGTTTATCTTTTTTAACTTGCTCAAAAAGGCGCTTAACCTTCAGTGATGTGTAATTCCTCCAGTcatgcat                                                             >  5:128998/1‑139 (MQ=255)                                                             tataAGCGTAATATTTAAGTGAAACTGGCCTTTTTCAGTGATAAGATCGCTATCGCTGTTCATCGCTTGTTTATCTTTTTTAACTTGATCAAAAAGGCGCTTAACCTTCAGTGATGTGTAATTCCTCCAGTcatgcatg                                                            >  6:25158/1‑139 (MQ=255)                                                               taAGCGTAATATTTAAGTGAAACTGGCCTTTTTCAGTGATAAGATCGCTATCGCTGTTCATCGCTTGTTTATCTTTTTTAACTTGCTCAAAAAGGCGCTTAACCTTCAGTg                                                                                      <  1:265052/111‑1 (MQ=255)                                                               taAGCGTAATATTTAAGTGAAACTGGCCTTTTTCAGTGATAAGATCGCTATCGCTGTTCATCGCTTGTTTATCTTTTTTAACTTGCTCAAAAAGGCGCTTAACCTTCAGTg                                                                                      >  2:265052/1‑111 (MQ=255)                                                                 aGCGTAATATTTAAGTGAAACTGGCCTTTTTCAGTGATAAGATCGCTATCGCTGTTCATCGCTTGTTTATCTTTTTTAACTTGCTCAAAAAGGCGCTTAACCTTCAGTg                                                                                      >  8:244541/1‑109 (MQ=255)                                                                 aGCGTAATATTTAAGTGAAACTGGCCTTTTTCAGTGATAAGATCGCTATCGCTGTTCATCGCTTGTTTATCTTTTTTAACTTGCTCAAAAAGGCGCTTAACCTTCAGTg                                                                                      <  7:244541/109‑1 (MQ=255)                                                                      aaTATTTAAGTGAAACTGGCCTTTTTCAGTGATAAGATCGCTATCGCTGTTCATCGCTTGTTTATCTTTTTTAACTTGCTCAAAAAGGCGCTTACCATTCAGTGATGTGTAATTCCTCCAGTCATGCATGCTGAAGcc                                                    >  2:290994/1‑138 (MQ=255)                                                                      aaTATTTAAGTGAAACTGGCCTTTTTCAGTGATAAGATCGCTATCGCTGTTCATCGCTTGTTTATCTTTTTTAACTTGCTCAAAAAGGCGCTTAACCTTCAGTGATGTGTAATTCCTCCAGTCATGCATGCTGATGcc                                                    >  7:253336/1‑138 (MQ=255)                                                                      aaTATTTAAGTGAAACTGGCCTTTTTCAGTGATAAGATCGCTATCGCTGTTAATCGCTGGTTTATCTTTTTTAACTTGCTCAAAAAGGCGCTTAACCTAAAGTGATGTGTAATTCCTCCAGTCATGCCTGCTGAAGcc                                                    >  2:214066/1‑138 (MQ=255)                                                                       atatTTAAGTGAAACTGGCCTTTTTCAGTGATAAGATCGCTATCGCTGTTCATCGCTTGTTTATCTTTTTTAACTTGCTCAAAAAGGCGCTTAACCTTCAGTGATGTGTAATTCCTCCAGt                                                                    <  1:76703/121‑1 (MQ=255)                                                                       atatTTAAGTGAAACTGGCCTTTTTCAGTGATAAGATCGCTATCGCTGTTCATCGCTTGTTTATCTTTTTTAACTTGCTCAAAAAGGCGCTTAACCTTCAGTGATGTGTAATTCCTCCAGt                                                                    >  2:76703/1‑121 (MQ=255)                                                                       atatTTAAGTGAAACTGGCCTTTTTCAGTGATAAGATCGCTATCGCTGTTCATCGCTTGTTTATCTTTTTTAACTTGCTCAAAAAGGCGCTTAACCTTCAGTGATGTGTAATTCCTCCAGTCATGCATGCTGAAGCCa                                                   >  1:216818/1‑138 (MQ=255)                                                                       atatTTAAGTGAAACTGGCCTTTTTCAGTGATAAGATCGCTATCGCTGTTCATCGCTTGTTTATCTTTTTTAACTTGCTCAAAAAGGCGCTTAACCTTCAGTGATGTGTAATTCCTCCAGTCATGCATGCTGAAGCCa                                                   >  1:123238/1‑138 (MQ=255)                                                                       atatTTAAGTGAAACTGGCCTTTTTCAGTGATAAGATCGCTATCGCTGTTCATCGCTTGTTTATCTTTTTTAACTTGCTCAAAAAGGCGCTTAACCTTCAGTGATGTGTAATTCCTCCAGTCATGCATGCTGAAGCCa                                                   >  3:150129/1‑138 (MQ=255)                                                                       atatTTAAGTGAAACTGGCCTTTTTCAGTGATAAGATCGCTATCGCTGTTCATCGCTTGTTTATCTTTTTTAACTTGCTCAAAAAGGCGCTTAACCTTCAGTGATGTGTAATTCCTCCAGTCATGCATGCTGAAGCCa                                                   >  2:25697/1‑138 (MQ=255)                                                                             aaGTGAAACTGGCCTTTTTCAGTGATAAGATCGCTATCGCTGTTCATCGCTTGTTTATCTTTTTTAACTTGCTCAAAAAGGCGCTTAACCTTCAGTGATGTGTAATTCCTCCAGTCATGCATGCTGAAGCCAAATTTg                                             <  5:56192/138‑1 (MQ=255)                                                                             aaGTGAAACTGGCCTTTTTCAGTGATAAGATCGCTATCGCTGTTCATCGCTTGTTTATCTTTTTTAACTTGCTCAAAAAGGCGCTTAACCTTCAGTGATGTGTAATTCCTCCAGTCATGCATGCTGAAGCCAAATTTGc                                            >  1:47419/1‑139 (MQ=255)                                                                                  aatcTGGCCTTTTTCAGTGATTAGATCGCTATATATGTTCATCGCTTGTTTATCTTTTTTATCTTGCTCAAAAAGGCGCTTAAGCTTCAGTGATGTGTAATTCCTCCAGTCATGCATGCTGAAGCCATATTTGCTGAtg                                       <  6:177987/136‑2 (MQ=255)                                                                                  aaaCTGGCCTTTTTCAGTGATAAGATCGCTATCGCTGTTCATCGCTTGTTTATCTTTTTTAACTTGCTCAAAAAGGCGCTTa                                                                                                >  5:257362/1‑82 (MQ=255)                                                                                  aaaCTGGCCTTTTTCAGTGATAAGATCGCTATCGCTGTTCATCGCTTGTTTATCTTTTTTAACTTGCTCAAAAAGGCGCTTa                                                                                                <  6:257362/82‑1 (MQ=255)                                                                                  aaaCTGGCCTTTTTCAGTGATAAGATCGCTATCGCTGTTCATCGCTTGTTTATCTTTTTTAACTTGCTCAAAAAGGCGCTTAACCTTCAGTGATGTGTAATTCCTCCAGTCATGCATGCTGAAGCCAAATTTGCTGATc                                       <  6:197517/139‑1 (MQ=255)                                                                                  aaaCTGGCCTTTTTCAGTGATAAGATCGCTATCGCTGTTCATCGCTTGTTTATCTTTTTTAACTTGCTCAAAAAGGCGCTTAACCTTCAGTGATGTGTAATTCCTCCAGTCATGCATGCTGAAGCCAAATTTGCTGATc                                       <  4:14150/139‑1 (MQ=255)                                                                                        gCCTTTTTCAGTGATAAGATCGCTATCGCTGTTTATCGCTTGTTTATCTTTTTTAACTTGCTCAAAAAGGCGCTTAACCTTCAGTGATGTGTAATTCCTCCAGTCATGCATGCTGAAGCCAAATTTGCTGATCATTTcc                                 >  2:159205/1‑139 (MQ=255)                                                                                        gCCTTTTTCAGTGATAAGATCGCTATCGCTGTTTATCGCTTGTTTATCTTTTTTAACTTGCTCAAAAAGGCGCTTAACCTTCAGTGATGTGTAATTCCTCCAGTCATGCATGCTGAAGCCAAATTTGCTGATCATTTcc                                 >  6:221199/1‑139 (MQ=255)                                                                                        gCCTTTTTCAGTGATAAGATCGCTATCGCTGTTCATCGCTTGTTTATCTTTTTTAACTTGCTCAAAAAGGCGCTTAACCTTCAGTGATGTGTAATTCCTCCAGTCATGCATGCTGAAGCCAAATTTGCTGATCAttt                                   <  6:128998/137‑1 (MQ=255)                                                                                             tttCAGTGATAAGATCGCTATCGCTGTTCATCGCTTGTTTATCTTTTTTAACTTGCTCAAAAAGGCGCTTAACCTTCAGTGATGTGTAATTCCTCCAGTCATGCATGCTGAAGCCAAATTTGCTGATCATTTCCTCAc                             <  2:47419/138‑1 (MQ=255)                                                                                               tCAGTGATAAGATCGGTCTCGCTGTTCATCGCTTGTTTATCTTTTTTAACTTGCTCCAAAAGGCGCTTAACCTTCAGTGATGTGTAATTGCTCCAGTCATGCATGCTGAAGCCAAATTTGCTGTTGATTTCCTCACCa                           <  4:71798/138‑1 (MQ=255)                                                                                                cAGTGATAAGATCGCTATCGCTGTTCATCGCGTGTTTATCTTTTTTAACTTGCTCAAAAAGGCGCTTAACCTTCAGTGATGTGTAATTCCTCCAGTCATGCATGGTGAAGCCAAATTTGCTGATCATTTCCTCACCAGc                         >  8:122137/1‑139 (MQ=255)                                                                                                  gTGATAAGATCGCTATCGCTGTTCATCGCTTGTTTATCTTTTTTAACTTGCTCAAAAAGGCGCTTAACCTTCAGTGATGTGTAATTCCTCCAGTCATGCATGCTGAAGCCAAATTTGCTGATCATTTCCTCACCAGCGt                       <  5:197998/139‑1 (MQ=255)                                                                                                   tGATAAGATCGCTATCGCTGTTCATCGCTTGTTTATCTTTTTTAACTTGCTCAAAAAGGCGCTTAACCTTCAGTGATGTGTAATtcctc                                                                        <  8:155088/89‑1 (MQ=255)                                                                                                   tGATAAGATCGCTATCGCTGTTCATCGCTTGTTTATCTTTTTTAACTTGCTCAAAAAGGCGCTTAACCTTCAGTGATGTGTAATtcctc                                                                        >  7:155088/1‑89 (MQ=255)                                                                                                        aGATCGCTATCGCTGTTCATCGCTTGTTTATCTTTTTTAACTTGCTCAAAAAGGCGCTTAACCTTCAGTGATGTGTAATTCCTCCAGTCATGCATGCTGAAGCCAAATTTGCTGATCATTTCCTCACCAGCGTGATTgg                 <  7:67951/139‑1 (MQ=255)                                                                                                         gATCGCTATCGCTGTTTATCGCTTGTTTATCTTTTTTAACTTGCTCAAAAAGGCGCTTAACCTTCAGTGATGTGTAATTCCTCCAGTCATGCATGCTGAAGCCAAATTTGCTGATCATTTCCTCACCAGCGTGATTGGa                <  1:159205/139‑1 (MQ=255)                                                                                                         gATCGCTATCGCTGTTTATCGCTTGTTTATCTTTTTTAACTTGCTCAAAAAGGCGCTTAACCTTCAGTGATGTGTAATTCCTCCAGTCATGCATGCTGAAGCCAAATTTGCTGATCATTTCCTCACCAGCGTGATTGGa                <  5:221199/139‑1 (MQ=255)                                                                                                          atcgctatcgctGTTCATCGCTTGTTTATCTTTTTTAACTTGCTCAAAAAGGCGCTTAACCTTCAGTGATGTGTAATTCCTCCAGTCATGCATGCTGAAGCCAAATTTGCTGATCATTTCCTCACCAGCGTGATTGGaa               <  5:216265/139‑1 (MQ=255)                                                                                                           tagctatcgctGTTCATCGCTTGTTTATCTTTTTTAACTTGCTCAAAAAGGCGCTTAACCTTCAGTGATGTGTAATTCCTCCAGTCATGCATGCTGAAGCCAAATTTGCTGATCATTTCCTCACCAGCGTGATTGGaa               <  6:176007/136‑1 (MQ=255)                                                                                                             gctatcgctGTTCATCGCTTGTTTATCTTTTTTAACTTGCTCAAAAAGgcgc                                                                                                   <  2:66479/52‑1 (MQ=255)                                                                                                             gctatcgctGTTCATCGCTTGTTTATCTTTTTTAACTTGCTCAAAAAGgcgc                                                                                                   >  1:66479/1‑52 (MQ=255)                                                                                                               tatcgctGTTCATCGCTTGTTTATCTTTTTTAACTTGCTCAAAAAGGCGCTTAACCTTCAGTGATGTGTAATTCCTCCAGTCATGCATGCTGAAGCCAAATTTGCTGATCATTTCCTCACCAGCGTGATTGGAAGatat          >  1:246472/1‑139 (MQ=255)                                                                                                                    ctGTTCATCGCTTGTTTATCTTTTTTAACTTGCTCAAAAAGGCGCTTAACCTTCAGTGATGTGTAATTCCTCCAGTCATGCATGCTGAAGCCAAATTTGCTGATCATTTCCTCACCAGCGTGATTGGAAGATATTATGt     <  4:150129/139‑1 (MQ=255)                                                                                                                       ttCATCGCTTGTTTATCTTTTTTAACTTGCTCAAAAAGGCGCTTAACCTTCAGTGATGTGTAATTCCTCCAGTCATGCATGCTGAAGCCAAATTTGCTGATCATTTCCTCACCAGCGTGATTGGAAGATATTATGtctc  >  5:78258/1‑139 (MQ=255)                                                                                                                         |                                                                                                                                         CTTCGTCATGTCTTTACAAATGACATAAATGCTGTCTAGTTCCTGATTGACCTCAACAGGTATAAGCGTAATATTTAAGTGAAACTGGCCTTTTTCAGTGATAAGATCGCTATCGCTGTTCATCGCTTGTTTATCTTTTTTAACTTGCTCAAAAAGGCGCTTAACCTTCAGTGATGTGTAATTCCTCCAGTCATGCATGCTGAAGCCAAATTTGCTGATCATTTCCTCACCAGCGTGATTGGAAGATATTATGTCTC  >  CP006881/1371690‑1371946

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑