Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 1,816,378 G→T 5.1% S817S (TCG→TCT)  U712_09030 → Polyketide synthase PksR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068811,816,3780GT 5.1% 273.2 / 3.8 79S817S (TCG→TCT) U712_09030Polyketide synthase PksR
Reads supporting (aligned to +/- strand):  ref base G (39/36);  new base T (2/2);  total (41/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.97e-01

ACCACTTTCGAAGGCGATGGAAGATCAGGATCATATTTACGCTGTGATCAAAAGCAGTTTTGCTAACCATAGCGGCGGAAGACAGATGTATACAGCTCCGGACCCGAAGCAGCAAGCAAAGTTAATTGTCAAGTCGATTCAGCAGTCGGGCATTGATCCAGAGACTATCGGCTATATTGAATCGGCGGCAAATGGTTCGGCGCTGGGCGATCCTATTGAAGTAATTGCCTTAACAAACGCGTTTCAACAATATACAAACAAGAAACAGTTTT  >  CP006881/1816243‑1816514
                                                                                                                                       |                                                                                                                                        
aCCACTTTCGAAGGCGATGGAAGATCAGGATCATATTTACGCTGTGATCAAAAGCAGTTTTGCTATCCATAGCGGCGGAAGACAGATGTATACAGCTCCGGACCCGAAGCAGCAAGCAAAGTTAATTGTCAAGTCGAt                                                                                                                                        >  4:65783/1‑138 (MQ=255)
aCCACTTTCGAAGGCGATGGAAGATCAGGATCATATTTACGCTGTGATCAAAAGCAGTTTTGCTAACCATAGCGGCGGAAGACAGATGTATACAGCTCCGGACCCGAAGCAGCAAGCAAAGTTAATTGTCAAGTCGAtt                                                                                                                                       >  1:280641/1‑139 (MQ=255)
   aCTTTCGAAGGCGATGGAAGATCAGGATCATATTTACGCTGTGATCAAAAGCAGTTTTGCTAACCATAGCGGCGGAAGACAGATGTATACAGCTCCGGACCCGAAGCAGCAAGCAAAGTTAATTGTCAAGTCGATTcag                                                                                                                                    <  6:54432/139‑1 (MQ=255)
   aCTTTCGAAGGCGATGGAAGATCAGGATCATATTTACGCTGTGATCAAAAGCAGTTTTGCTAACCATAGCGGCGGAAGACAGATGTATACAGCTCCGGACCCGAAGCAGCAAGCAAAGTTAATTGTCAAGTCGATTcag                                                                                                                                    <  7:273079/139‑1 (MQ=255)
                 tGGAAGATCAGGATCATATTTACGCTGTGATCAAAAGCAGTTTTGCTAACCATAGCGGCGGAAGACAGATGTATACAGCTCCGGACCCGAAGCAGCAAGCAAAGTTAATTGTCAAGTCGATTCAGCAGTCGGGCATTGa                                                                                                                      >  1:101773/1‑139 (MQ=255)
                       aTCAGGATCATATTTACGCTGTGATCAAAAGCAGTTTTGCTAACCATAGCGGCGGAAGACAGATGTATACAGCTCCGGACCCGAAGCAGCAAGCAAAGTTAATTGTCAAGTCGATTCAGCAGTCGGGCATTGATCCa                                                                                                                  >  8:9813/1‑137 (MQ=255)
                           ggATCATATTTACGCTGTGATCAAAAGCAGTTTTGCTAACCATAGCGGCGGAAGACAGATGTATACAGCTCCGGACCCGAAGCAGCAAGCAAAGTTAATTGTCAAGTCGATTCAGCAGTCGGGCATTGATCCAGAGACt                                                                                                            <  3:114424/139‑1 (MQ=255)
                             aTCATATTTACGCTGTGATCAAAAGCAGTTTTGCTAACCATAGCGGCGGAAGACAGATGTATACAGCTCCGGACCCGAAGCAGCAAGCAAAGTTAATTGTCAAGTCGATTCAGCAGTCGGGCATTGATCCAGAGACTAt                                                                                                          <  3:128882/139‑1 (MQ=255)
                                      aCGCTGTGATCAAAAGCAGTTTTGCTAACCATAGCGGCGGAAGACAGATGTATACAGCTCCGGACCCGAAGCAGCAAGCAAAGTTAATTGTCAAGTCGATTCAGCAGTCGGGCATTGATCCAGAGACTATCGGCTatat                                                                                                 >  7:129922/1‑139 (MQ=255)
                                            tgATCAAAAGCAGTTTTGCTAACCATAGCGGCGGAAGACAGATGTATACAGCTCCGGACCCGAAGCAGCAAGCAAAGTTAATTGTCAAGTCGATTCAGCAGTCGGGCa                                                                                                                          >  1:194521/1‑108 (MQ=255)
                                            tgATCAAAAGCAGTTTTGCTAACCATAGCGGCGGAAGACAGATGTATACAGCTCCGGACCCGAAGCAGCAAGCAAAGTTAATTGTCAAGTCGATTCAGCAGTCGGGCa                                                                                                                          <  2:194521/108‑1 (MQ=255)
                                            tgATCAAAAGCAGTTTTGCTAACCATAGCGGCGGAAGACAGATGTATACAGCTCCGGACCCGAAGCAGCAAGCAAAGTTAATTGTCAAGTCGATTCAGCAGTCGGGCATTGATCCAGAGACTATCGGCTATATTGAATc                                                                                           >  6:6375/1‑139 (MQ=255)
                                            tgATCAAAAGCAGTTTTGCTAACCATAGCGGCGGAAGACAGATGTATACAGCTCCGGACCCGAAGCAGCAAGCAAAGTTAATTGTCAAGTCGATTCAGCAGTCGGGCATTGATCCAGAGACTATCGGCTATATTGAATc                                                                                           >  5:38280/1‑139 (MQ=255)
                                            tgATCAAAAGCAGTTTTGCTAACCATAGCGGCGGAAGACAGATGTATACAGCTCCGGACCCGAAGCAGCAAGCAAAGTTAATTGTCAAGTCGATTCAGCAGTCGGGCATTGATCCAGAGACTATCGGCTATATTGAATc                                                                                           >  8:140765/1‑139 (MQ=255)
                                            tgATCAAAAGCAGTTTTGCTAACCATAGCGGCGGAAGACAGATGTATACAGCTCCGGACCCGAAGCAGCAAGCAAAGTTAATTGTCAAGTCGAATCAGCAGTCGGGCATTGATCCAGAGACTAt                                                                                                          >  7:6842/1‑124 (MQ=255)
                                            tgATCAAAAGCAGTTTTGCTAACCATAGCGGCGGAAGACAGATGTATACAGCTCCGGACCCGAAGCAGCAAGCAAAGTTAATTGTCAAGTCGAATCAGCAGTCGGGCATTGATCCAGAGACTAt                                                                                                          <  8:6842/124‑1 (MQ=255)
                                              aTCAAAAGCAGTTTTGCTAACCATAGCGGCGGAAGACAGATGTATACAGCTCCGGACCCGAAGCAGCAAGCAAAGTTAATTGTCAAGTCGATTCAGCAGTCGGGCATTGATCCAGAGACTATCGGCTATATTGAATcgg                                                                                         >  5:13848/1‑139 (MQ=255)
                                              aTCAAAAGCAGTTTTGCTAACCATAGCGGCGGAAGACAGATGTATACAGCTCCGGACCCGAAGCAGCAAGCAAAGTTAATTGTCAAGTCGATTCAGCAGGCGGGCATTGATCCAGAGACTATCGGCTATATTGAATcgg                                                                                         <  2:280641/139‑1 (MQ=255)
                                                 aaaaGCAGTTTTGCTAACCATAGCGGCGGAAGACAGATGTATACAGCTCCGGACCCGAAGCAGCAAGCAAAGTTAATTGTCAAGTCGATTCAGCAGTCGGGCATTGATCCAGAGACTATCGGCTATATTGAATcggcg                                                                                       <  8:129922/138‑1 (MQ=255)
                                                 aaaaGCAGTTTTGCTAACCATAGCGGCGGAAGACAGATGTATACAGCTCCGGACCCGAAGCAGCAAGCAAAGTTAATTGTCAAGTCGATTCAGCAGTCGGGCATTGATCCAGAGACTATCGGCTATATTGAATcggcg                                                                                       <  2:257354/138‑1 (MQ=255)
                                                        gTTTTGCTAACCATAGCGGCGGAAGACAGATGTATACAGCTCCGGACCCGAAGCAGCAAGCAAAGTTAATTGTCAAGTCGATTCAGCAGTCGGGCATTGATCCAGAGACTATCGGCTATATTGAATCGGCGGCAAATgg                                                                               >  4:238408/1‑139 (MQ=255)
                                                                 aCCATAGCGGCGGAAGACAGATGTATACAGCTCCGGACCCGAAGCAGCAAGCAAAGTTAATTGTCAAGTCGATTCAGCAGTCGGGCATTGATCCAGAGACTATCGGCTATATTGAATCGGCGGCAAATGGTTCGgcg                                                                        <  3:238408/137‑1 (MQ=255)
                                                                      aGCGGCGGAAGACAGATGTATACAGCTCCGGACCCGAAGCAGCAAGCAAAGTTAATTGTCAAGTCTATTCAGCAGTCGGGCATTGATCCAGAGACTATCGGCTATATTGAATCGGCGGCAAATGGtt                                                                             <  1:99576/127‑1 (MQ=255)
                                                                      aGCGGCGGAAGACAGATGTATACAGCTCCGGACCCGAAGCAGCAAGCAAAGTTAATTGTCAAGTCTATTCAGCAGTCGGGCATTGATCCAGAGACTATCGGCTATATTGAATCGGCGGCAAATGGtt                                                                             >  2:103876/1‑127 (MQ=255)
                                                                      aGCGGCGGAAGACAGATGTATACAGCTCCGGACCCGAAGCAGCAAGCAAAGTTAATTGTCAAGTCTATTCAGCAGTCGGGCATTGATCCAGAGACTATCGGCTATATTGAATCGGCGGCAAATGGtt                                                                             >  2:99576/1‑127 (MQ=255)
                                                                      aGCGGCGGAAGACAGATGTATACAGCTCCGGACCCGAAGCAGCAAGCAAAGTTAATTGTCAAGTCTATTCAGCAGTCGGGCATTGATCCAGAGACTATCGGCTATATTGAATCGGCGGCAAATGGtt                                                                             <  1:103876/127‑1 (MQ=255)
                                                                      aGCGGCGGAAGACAGATGTATACAGCTCCGGACCCGAAGCAGCAAGCAAAGTTAATTGTCAAGTCGATTCAGCAGTCGGGCATTg                                                                                                                       >  7:193721/1‑85 (MQ=255)
                                                                      aGCGGCGGAAGACAGATGTATACAGCTCCGGACCCGAAGCAGCAAGCAAAGTTAATTGTCAAGTCGATTCAGCAGTCGGGCATTg                                                                                                                       <  8:193721/85‑1 (MQ=255)
                                                                         ggcggAAGACAGATGTATACAGCTCCGGACCCGAAGCAGCAAGCAAAGTTAATTGTCAAGTCGATTCAGCAGTCGGGCATTGATCCAGAGACTATCGGCTATATTGAATCGGCGGCAAATGGTTCGGCGCTGGGCGATc                                                              <  8:145194/139‑1 (MQ=255)
                                                                             gAAGACAGATGTATACAGCTCCGGACCCGAAGCAGCAAGCAAAGTTAATTGTCAAGTCGATTCAGCAGTCGGGCATTGATCCAGAGACTATCGGCTATATTGAATCGGCGGCAAATGGTTCGGCGCTGGGCGATCCTAt                                                          >  5:106837/1‑139 (MQ=255)
                                                                               aGACAGATGTATACAGCTCCGGACCCGAAGCAGCAAGCAAAGTTAATTGTCAAGTCGATTCAGCAGTCGGGCATTGATCCAGAGACTATCGGCTATATTGAATCGGCGGCAAATGGTTCGGCGCTGGGCGATCCTATTg                                                        <  3:65783/139‑1 (MQ=255)
                                                                                       gTATACAGCTCCGGACCCGAAGCAGCAAGCAAAGTTAATTGTCAAGTCGAtt                                                                                                                                       >  8:140895/1‑52 (MQ=255)
                                                                                       gTATACAGCTCCGGACCCGAAGCAGCAAGCAAAGTTAATTGTCAAGTCGAtt                                                                                                                                       <  7:140895/52‑1 (MQ=255)
                                                                                          taCAGCTCCGGACCCGAAGCAGCAAGCAAAGTTAATTGTCAAGTCGATTCAGCAGTCGGGCATTGATCCAGAGACTATCGGCTATATTGAATCGGCGGCAAATGGTTCGGCGCTGGGCGATCCTATTGAAGTAATTGcc                                             >  2:247148/1‑139 (MQ=255)
                                                                                          taCAGCTCCGGACCCGAAGCAGCAAGCAAAGTTAATTGTCAAGTCGATTCAGCAGTCGGGCATTGATCCAGAGACTATCGGCTATATTGAATCGGCGGCAAATGGTTCGGCGCTGGGCGATCCTATTGAAGTAATTGc                                              <  3:48094/138‑1 (MQ=255)
                                                                                             aGCTCCGGCCCCGAAGCAGCAAGCAAAGTTAATTGTCAAGTCGATTCAGCAGTCGGGCATTGATCCAGAGCCGATCGGCTTTATTGCATCGGCGGCAAATGGTTCGGCGCTGGGCGAGCCTATGGAAGTATTTGCCtt                                           >  2:258590/1‑138 (MQ=255)
                                                                                              gCTCCGGACCCGAAGCAGCAAGCAAAGTTAATTGTCAAGTCGATTCAGCAGTCGGGCATTGATCCAGAGACTATCGGCTATATTGAATCGGCGGCAAATGGTTCGGCGCTGGGCGATCCTATTGAAGTAATTGCCTTaa                                         >  1:123174/1‑139 (MQ=255)
                                                                                               cTCCGGACCCGAAGCAGCAAGCAAAGTTAATTGTCAAGTCGATTCAGCAGTCGGGCATTGATCCAGAGACTATCGGCTATATTGAATCGGCGGCaaa                                                                                  >  5:220188/1‑97 (MQ=255)
                                                                                               cTCCGGACCCGAAGCAGCAAGCAAAGTTAATTGTCAAGTCGATTCAGCAGTCGGGCATTGATCCAGAGACTATCGGCTATATTGAATCGGCGGCaaa                                                                                  <  6:220188/97‑1 (MQ=255)
                                                                                               cTCCGGACCCGAAGCAGCAAGCAAAGTTAATTGTCAAGTCGATTCAGCAGTCGGGCATTGATCCAGAGACTATCGGCTATATTGAATCGGCGGCAAATGGTTCGGCGCTggg                                                                   >  8:136645/1‑112 (MQ=255)
                                                                                               cTCCGGACCCGAAGCAGCAAGCAAAGTTAATTGTCAAGTCGATTCAGCAGTCGGGCATTGATCCAGAGACTATCGGCTATATTGAATCGGCGGCAAATGGTTCGGCGCTggg                                                                   <  7:136645/112‑1 (MQ=255)
                                                                                               cTCCGGACCCGAAGCAGCAAGCAAAGTTAATTGTCAAGTCGATTCAGCAGTCGGGCATTGATCCAGAGACTATCGGCTATATTGAATCGGCGGCAAATGGTTCGGCGCTGGGCGa                                                                >  5:273522/1‑115 (MQ=255)
                                                                                               cTCCGGACCCGAAGCAGCAAGCAAAGTTAATTGTCAAGTCGATTCAGCAGTCGGGCATTGATCCAGAGACTATCGGCTATATTGAATCGGCGGCAAATGGTTCGGCGCTGGGCGa                                                                <  6:273522/115‑1 (MQ=255)
                                                                                               cTCCGGACCCGAAGCAGCAAGCAAAGTTAATTGTCAAGTCGATTCAGCAGTCGGGCATTGATCCAGAGACTATCGGCTATATTGAATCGGCGGCAAATGGTTCGGCGCTGGGCGATCCTATTg                                                        >  4:3299/1‑123 (MQ=255)
                                                                                               cTCCGGACCCGAAGCAGCAAGCAAAGTTAATTGTCAAGTCGATTCAGCAGTCGGGCATTGATCCAGAGACTATCGGCTATATTGAATCGGCGGCAAATGGTTCGGCGCTGGGCGATCCTATTg                                                        <  3:3299/123‑1 (MQ=255)
                                                                                               cTCCGGACCCGAAGCAGCAAGCAAAGTTAATTGTCAAGTCGATTCAGCAGTCGGGCATTGATCCAGAGACTATCGGCTATATTGAATCGGCGGCAAATGGTTCGGCGCTGGGCGATCCTATTGAAGTAATTGCCTTaa                                         >  1:19761/1‑138 (MQ=255)
                                                                                               cTCCGGACCCGAAGCAGCAAGCAAAGTTAATTGTCAAGTCGATTCAGCAGTCGGGCATTGATCCAGAGACTATCGGCTATATTGAATCGGCGGCAAATGGTTCGGCGCTGGGCGATCCTATTGAAGTAATTGCCTTaa                                         >  7:270435/1‑138 (MQ=255)
                                                                                               cTCCGGACCCGAAGCAGCAAGCAAAGTTAATTGTCAAGTCGATTCAGCAGTCGGGCATTGATCCAGAGACTATCGGCTATATTGAATCGGCGGCAAATGGTTCGGCGCTGGGCGATCCTATTGAAGTAATTGCCTTaa                                         >  2:25068/1‑138 (MQ=255)
                                                                                               cTCCGGACCCGAAGCAGCAAGCAAAGTTAATTGTCAAGTCGATTCAGCAGTCGGGCATTGATCCAGAGACTATCGGCTATATTGAATCGGCGGCAAATGGTTCGGCGCTGGGCGATCCTATTGAAGTAATTGCCTTaa                                         >  2:4705/1‑138 (MQ=255)
                                                                                               cTCCGGACCCGAAGCAGCAAGCAAAGTTAATTGTCAAGTCGATTCAGCAGTCGGGCATTGATCCAGAGACTATCGGCTATATTGAATCGGCGGCAAATGGTTCGGCGCTGGGCGATCCTATTGAAGTAATTGCCTTaa                                         >  5:103820/1‑138 (MQ=255)
                                                                                               cTCCGGACCCGAAGCAGCAAGCAAAGTTAATTGTCAAGTCGATTCAGCAGTCGGGCATTGATCCAGAGACTATCGGCTATATTGAATCGGCGGCAAATGGTTCGGCGCTGGGCGATCCTATTGAAGTAATTGCCTTaa                                         >  2:220954/1‑138 (MQ=255)
                                                                                               cTCCGGACCCGAAGCAGCAAGCAAAGTTAATTGTCAAGTCGATTCAGCAGTCGGGCATTGATCCAGAGACTATCGGCTATATTGAATCGGCGGCAAATGGTTCGGCGCTGGGCGATCCTATTGAAGTAATTGCCTTaa                                         >  3:115668/1‑138 (MQ=255)
                                                                                               cTCCGGACCCGAAGCAGCAAGCAAAGTTAATTGTCAAGTCGATTCAGCAGTCGGGCATTGATCCAGAGACTATCGGCTATATTGAATCGGCGGCAAATGGTTCGGCGCTGGGCGATCCTATTGAAGTAATTGCCTTAAc                                        >  3:89318/1‑139 (MQ=255)
                                                                                                  cGGACCCGAAGCAGCAAGCAAAGTTAATTGTCAAGTCGATTCAGCAGTCGGGCATTGATCCAGAGACTATCGGCTATATTGAATCGGCGGCAAATGGTTCGGCGCTGGGCGa                                                                >  2:150135/1‑112 (MQ=255)
                                                                                                  cGGACCCGAAGCAGCAAGCAAAGTTAATTGTCAAGTCGATTCAGCAGTCGGGCATTGATCCAGAGACTATCGGCTATATTGAATCGGCGGCAAATGGTTCGGCGCTGGGCGa                                                                <  1:150135/112‑1 (MQ=255)
                                                                                                  cGGACCCGAAGCAGCAAGCAAAGTTAATTGTCAAGTCGATTCAGCAGTCGGGCATTGATCCAGAGACTATCGGCTATATTGAATCGGCGGCAAATGGTTCGGCGCTGGGCGATCCTATTGAAGTAATTGCCTTAACaaa                                     <  2:171480/139‑1 (MQ=255)
                                                                                                  cGGACCCGAAGCAGCAAGCAAAGTTAATTGTCAAGTCGATTCAGCAGTCGGGCATTGATCCAGAGACTATCGGCTATATTGAATCGGCGGCAAATGGTTCGGCGCTGGGCGATCCTATTGAAGTAATTGCCTTAACaaa                                     >  5:27910/1‑139 (MQ=255)
                                                                                                  cGGACCCGAAGCAGCAAGCAAAGTTAATTGTCAAGTCGATTCAGCAGTCGGGCATTGATCCAGAGACTATCGGCTATATTGAATCGGCGGCAAATGGTTCGGCGCTGGGCGATCCTATTGAAGTAATTGCCTTAACaaa                                     <  1:4705/139‑1 (MQ=255)
                                                                                                    gACCCGAAGCAGCAAGCAAAGTTAATTGTCAAGTCGATTCAGCAGTCGGGCATTGATCCAGAGACTATCGGCTATATTGAATCGGCGGCAAATGGTTCGGCGCTGGGCGATCCTATTGAAGTAATTGCCTTAACAAAcg                                   >  8:26131/1‑139 (MQ=255)
                                                                                                       ccGAAGCAGCAAGCAAAGTTAATTGTCAAGTCGATTCAGCAGTCGGGCATTGATCCAGAGACTATCGGCTATATTGAATCGGCGGCAAATGGTTCGGCGCTGGGCGATCCTATTGAAGTAATTGCCTTAACAAACGCGt                                <  6:106837/139‑1 (MQ=255)
                                                                                                         gAAGCAGCAAGCAAAGTTAATTGTCAAGTCGATTCAGCAGTCGGGCATTGATCCAGAGACTATCGGCTATATTGAATCGGCGGCAAATGGTTCGGCGCTGGGCGATCCTATTGAAGTAATTGCCTTAACAAACGCGttt                              <  1:247148/139‑1 (MQ=255)
                                                                                                         gAAGCAGCAAGCAAAGTTAATTGTCAAGTCGATTCAGCAGTCGGGCATTGATCCAGAGACTATCGGCTATATTGAATCGGCGGCAAATGGTTCGGCGCTGGGCGATCCTATTGAAGTAATTGCCTTAACAAACGCGttt                              <  1:25068/139‑1 (MQ=255)
                                                                                                         gAAGCAGCAAGCAAAGTTAATTGTCAAGTCGATTCAGCAGTCGGGCATTGATCCAGAGACTATCGGCTATATTGAATCGGCGGCAAATGGTTCGGCGCTGGGCGATCCTATTGAAGTAATTGCCTTAACAAACGCGttt                              >  8:257564/1‑139 (MQ=255)
                                                                                                         gAAGCAGCAAGCAAAGTTAATTGTCAAGTCGATTCAGCAGTCGGGCATTGATCCAGAGACTATCGGCTATATTGAATCGGCGGCAAATGGTTCGGCGCTGGGCGATCCTATTGAAGTAATTGCCTTAACAAACGCGttt                              <  1:220954/139‑1 (MQ=255)
                                                                                                             cagcaAGCAAAGTTAATTGTCAAGTCGATTCAGCAGTCGGGCATTGATCCAGAGACTATCGGCTATATTGAATCGGCGGCAAATGGTTCGGCGCTGGGCGATCCTATTGAAGTAATTGCCTTAACAAACGCGTTTcaac                          <  7:26131/139‑1 (MQ=255)
                                                                                                              agcaagcaAAGTTAATTGTCAAGTCGATTCAGCAGTCGGGCATTGATCCAGAGACTATCGGCTATATTGAATCGGCGGCAAATGGTTCGGCGCTGGGCGATCCTATTGAAGTAATTGCCTTAACAAACGCGTTTcaaca                         <  7:257564/139‑1 (MQ=255)
                                                                                                                caagcaaAGTTAATTGTCAAGTCGATTCAGCAGTCGGGCATTGATCCAGAGACTATCGGCTATATTGAATCGGCGGCAAATGGTTCGGCGCTGGGCGATCCTATTGAAGTAATTGCCTTAACAAACGCGTTTCAACAat                       <  7:140765/139‑1 (MQ=255)
                                                                                                                     aaaGTTAATTGTCAATTCGATTCAGCAGGCGGGCATTGATCCAGAGACTATCGGCTATATTGAATCGGCGGCAAATGGTTCGGCGCTGGGCGATCCTATTGAAGTAATTGCCTTAACAAACGCGTTTCAACAATATaca                  <  5:198147/139‑1 (MQ=255)
                                                                                                                         ttAATTGTCAAGTCGATTCAGCAGTCGGGCATTGATCCAGAGACTATCGGCTATATTGAATCGGCGGCAAATGGTTCGGCGCTGGGCGATCCTATTGAAGTAATTGCCTTAACAAACGCGTTTCAACAATATacaaaca              <  7:9813/139‑1 (MQ=255)
                                                                                                                         ttAATTGTCAAGTCGATTCAGCAGTCGGGCATTGATCCAGAGACTATCGGCTATATTGAATCGGCGGCAAATGGTTCGGCGCTGGGCGATCCTATTGAAGTAATTGCCTTAACAAACGCGTTTCAACAATATacaaaca              <  6:287913/139‑1 (MQ=255)
                                                                                                                         ttAATTGTCAAGTCGATTCAGCAGTCGGGCATTGATCCAGAGACTATCGGCTATATTGAATCGGCGGCAAATGGTTCGGCGCTGGGCGATCCTATTGAAGTAATTGCCTTAACAAACGCGTTTCAACAATATacaaaca              <  6:287215/139‑1 (MQ=255)
                                                                                                                            aTTGTCAAGTCGATTCAGCAGTCGGGCATTGATCCAGAGACTATCGGCTATATTGAATCGGCGGCAAATGGTTCGGCGCTGGGCGATCCTAATGAAGTAATTGCCTTAACAAACGCGTTTCAACAATATACAAACAAg            >  6:182652/1‑138 (MQ=255)
                                                                                                                                 cAAGTCGATTCAGCAGTCGGGCATTGATCCAGAGACTATCGGCTATATTGAATCGGCGGCAAATGGTTCGGCGCTGGGCGATCCTATTGAAGTAATTGCCTTAACAAACGCGTTTCAACAATATACAAACAAGAAACAg      >  5:110024/1‑139 (MQ=255)
                                                                                                                                  aaGTCGATTCAGCAGTCGGGCATTGATCCAGAGACTATCGGCTATATTGAATCGGCGGCAAATGGTTCGGCGCTGGGCGATCCTATTGAAGTAATTGCCTTAACAAACGCGTTTCAACAATATACAAACAAGAAACAGt     >  6:132005/1‑139 (MQ=255)
                                                                                                                                  aaGTCGATTCAGCAGTCGGGCATTGATCCAGAGACTATCGGCTATATTGAATCGGCGGCAAATGGTTCGGCGCTGGGCGATCCTATTGAAGTAATTGCCTTAACAAACGCGTTTCAACAATATACAAACAAGAAACAGt     >  6:70268/1‑139 (MQ=255)
                                                                                                                                   aGTCGATTCAGCAGTCGGGCATTGATCCAGAGACTATCGGCTATATTGAATCGGCGGCAAATGGTTCGGCGCTGGGCGATCCTATTGAAGTAATTGCCTTAACAAACGCGTTTCAACAATATACAAACAAGAAACAGtt    <  5:132005/139‑1 (MQ=255)
                                                                                                                                   aGTCGATTCAGCAGTCGGGCATTGATCCAGAGACTATCGGCTATATTGAATCGGCGGCAAATGGTTCGGCGCTGGGCGATCCTATTGAAGTAATTGCCTTAACAAACGCGTTTCAACAATATACAAACAAGAAACAGtt    >  5:221566/1‑139 (MQ=255)
                                                                                                                                   aGTCGATTCAGCAGTCGGGCATTGATCCAGAGACTATCGGCTATATTGAATCGGCGGCAAATGGTTCGGCGCTGGGCGATCCTATTGAAGTAATTGCCTTAACAAACGCGTTTCAACAATATACAAACAAGAAACAGtt    <  5:70268/139‑1 (MQ=255)
                                                                                                                                     tCGATTCAGCAGTCGGGCATTGATCCAGAGACTATCGGCTATATTGAATCGGCGGCAAATGGTTCGGCGCTGGGCGATCCTATTGAAGTAATTGCCTTAACAAACGCGTTTCAACAATATACAAACAAGAAACAGtttt  <  6:27910/139‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                        
ACCACTTTCGAAGGCGATGGAAGATCAGGATCATATTTACGCTGTGATCAAAAGCAGTTTTGCTAACCATAGCGGCGGAAGACAGATGTATACAGCTCCGGACCCGAAGCAGCAAGCAAAGTTAATTGTCAAGTCGATTCAGCAGTCGGGCATTGATCCAGAGACTATCGGCTATATTGAATCGGCGGCAAATGGTTCGGCGCTGGGCGATCCTATTGAAGTAATTGCCTTAACAAACGCGTTTCAACAATATACAAACAAGAAACAGTTTT  >  CP006881/1816243‑1816514

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑