Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 1,966,649 C→T 6.4% C80Y (TGC→TAC)  U712_09710 ← Glutamate synthase [NADPH] large chain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068811,966,6490CT 6.4% 202.9 / 2.4 62C80Y (TGC→TAC) U712_09710Glutamate synthase [NADPH] large chain
Reads supporting (aligned to +/- strand):  ref base C (23/35);  new base T (2/2);  total (25/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATCCAAGAACGACTTGGCCTTCTTGTTCAATTAGTGCATTGATTTGCTTTTCAATTTTTTTTCTTTCATCTTCCTTCTGTGAGAAAAAGACCATTCCTACCCCGTAACGTTCTTTTTCTGGCAGATTGATGTTTTTGCACTCTTTTCTAAAGAAAGCATCAGGGATTTGAACCAGTAAGCCGGCTCCGTCTCCTGTATCCGGATCACTGCCTTGCCCTCCTCTATGGTCTAGCTGGCAAAGCATCTTAAGTCCTTGTTTGAC  >  CP006881/1966512‑1966773
                                                                                                                                         |                                                                                                                            
atccaAGAACGACTTGGCCTTCTTGTTCAATTAGTGCATTGATTTGCTTTTCAATTTTTTTTCTTTCATCTTCCTTCTGTGAGAAAAAGACCATTCCTACCCCGTAACGTTCTTTTTCTGGCAGATTGATGTTTTTGCa                                                                                                                             <  1:240250/139‑1 (MQ=255)
    aaGAACGACTTGGCCTTCTTGTTCAATTAGTGCATTGATTTGCTTTTCAATTTTTTTTCTTTCATCTTCCTTCTGTGAGAAAAAGACCATTCCTACCCCGTAACGTTCTTTTTCTGGCAGATTGATGTTTTTGCActct                                                                                                                         >  7:97592/1‑139 (MQ=255)
    aaGAACGACTTGGCCTTCTTGTTCAATTAGTGCATTGATTTGCTTTTCAATTTTTTTTCTTTCATCTTCCTTCTGTGAGAAAAAGACCATTCCTACCCCGTAACGTTCTTTTTCTGGCAGATTGATGTTTTTGCActct                                                                                                                         >  2:8912/1‑139 (MQ=255)
       aaCGACTTGGACTTCTTGTTCAATTAGTGCATTGATTTGCTTTTCAATTTTTTTTCTTTCATCTTCCTTATGTGAGAAAAAGACCATTCCTACCCCGTAACGTTCTTTTTCTGGCAGATTGATGTTTTTGCACTCtttt                                                                                                                      <  2:138469/139‑1 (MQ=255)
           aCTTGGCCTTCTTGTTCAATTAGTGCATTGATTTGCTTTTCAATTTTTTTTCTTTCATCTTCCTTCTGTGAGAAAAAGACCATTCCTACCCCGTAACGTTCTTTTTCTGGCAGATTGATGTTTTTGCACTCTTTTCTa                                                                                                                   >  6:79710/1‑138 (MQ=255)
            cTTGGCCTTCTTGTTCAATTAGTGCATTGATTTGCTTTTCAATTTTTTTTCTTTCATCTTCCTTCTGTGAGAAAAAGACCATTCCTACCCCGTAACGTTCTTTTTCTGGCAGATTGATGTTTTTGCACTCTTTTCTaaa                                                                                                                 >  2:83058/1‑139 (MQ=255)
            cTTGGCCTTCTTGTTCAATTAGTGCATTGATTTGCTTTTCAATTTTTTTTCTTTCATCTTCCTTCTGTGAGAAAAAGACCATTCCTACCCCGTAACGTTCTTTTTCTGGCAGATTGATGTTTTTGCACTCTTTTCTaa                                                                                                                  >  2:288308/1‑138 (MQ=255)
               ggCCTTCTTGTTCAATTAGTGCATTGATTTGCTTTTCAATTTTTTTTCTTTCATCTTCCTTCTGTGAGAAAAAGACCATTCCTACCCCGTAACGTTCTTTTTCTGGCAGATTGATGTTTTTGCACTCTTTTCTaaaga                                                                                                               <  3:55968/138‑1 (MQ=255)
                gCCTTCTTGTTCAATTAGTGCATTGATTTGCTTTTCAATTTTTTTTCTTTCATCTTCCTTCTGTGAGAAAAAGACCATTCCTACCCCGTAACGTTCTTTTTCTGGCAGATTGATGTTTTTGCACTCTTTTCTaaagaa                                                                                                              <  1:288308/138‑1 (MQ=255)
                gCCTTCTTGTTCAATTAGTGCATTGATTTGCTTTTCAATTTTTTTTCTTTCATCTTCCTTCTGTGAGAAAAAGACCATTCCTACCCCGTAACGTTCTTTTTCTGGCAGATTGATGTTTTTGCACTCTTTTCTaaagaa                                                                                                              <  1:83058/138‑1 (MQ=255)
                gCCTTCTTGTTCAATTAGTGCATTGATTTGCTTTTCAATTTTTTTTCTTTCATCTTCCTTCTGTGAGAAAAAGACCATTCCTACCCCGTAACGTTCTTTTTCTGGCAGATTGATGTTTTTGCACTCTTTTCTaaagaa                                                                                                              <  2:118937/138‑1 (MQ=255)
                gCCTTCTTGTTCAATTAGTGCATTGATTTGCTTTTCAATTTTTTTTCTTTCATCTTCCTTCTGTGAGAAAAAGACCATTCCTACCCCGTAACGTTCTTTTTCTGGCAGATTGATGTTTTTGCACTCTTTTCTaaagaa                                                                                                              <  6:94855/138‑1 (MQ=255)
                            aaTTAGTGCATTGATTTGCTTTTCAATTTTTTTTCTTTCATCTTCCTTCTGTGAGAAAAAGACCATTCCTACCCCGTAACGTTCTTTTTCTGGCAGATTGATGTTTTTGCACTCTTTTCTAAAGAAAGCATCAGGGAt                                                                                                  <  1:28761/138‑1 (MQ=255)
                              ttAGTGCATTGATTTGCTTTTCAATTTTTTTTCTTTCATCTTCCTTCTGTGAGAAAAAGACCATTCCTACCCCGTAACGTTCTTTTTCTGGCAGATTGATGTTTTTGCACTCTTTTCTAAAGAAAGCATCAGGGATTTg                                                                                               <  1:188484/139‑1 (MQ=255)
                                  tGCATTGATTTGCTTTTCAATTTTTTTTCTTTCATCTTCCTTCTGTGAGAAAAAGACCATTCCTACCCCGTAACGTTCTTTTTCTGGCAGATTGATGTTTTTGTACTCTTTTCTAAAGAAAGCATCa                                                                                                       >  7:5475/1‑127 (MQ=255)
                                  tGCATTGATTTGCTTTTCAATTTTTTTTCTTTCATCTTCCTTCTGTGAGAAAAAGACCATTCCTACCCCGTAACGTTCTTTTTCTGGCAGATTGATGTTTTTGTACTCTTTTCTAAAGAAAGCATCa                                                                                                       <  8:1194/127‑1 (MQ=255)
                                  tGCATTGATTTGCTTTTCAATTTTTTTTCTTTCATCTTCCTTCTGTGAGAAAAAGACCATTCCTACCCCGTAACGTTCTTTTTCTGGCAGATTGATGTTTTTGTACTCTTTTCTAAAGAAAGCATCa                                                                                                       <  8:5475/127‑1 (MQ=255)
                                  tGCATTGATTTGCTTTTCAATTTTTTTTCTTTCATCTTCCTTCTGTGAGAAAAAGACCATTCCTACCCCGTAACGTTCTTTTTCTGGCAGATTGATGTTTTTGTACTCTTTTCTAAAGAAAGAATCa                                                                                                       >  7:1194/1‑127 (MQ=255)
                                  tGCATTGATTTGCTTTTCAATTTTTTTTCTTTCATCTTCCTTCTGTGAGAAAAAGACCATTCCTACCCCGTAACGTTCTTTTTCTGGCAGATTGATGTTTTTGCACTCTTTTCTAAAGAAAGCATCAGGGATTTGAAc                                                                                            >  5:252027/1‑138 (MQ=255)
                                   gCATTGATTTGCTTTTCAATTTTTTTTCTTTCATCTTCCTTCTGTGAGAAAAAGACCATTCCTACCCCGTAACGTTCTTTTTCTGGCAGATTGATGTTTTTGCACTCTTTTCTAAAGAAAGCATCa                                                                                                       <  8:28264/126‑1 (MQ=255)
                                   gCATTGATTTGCTTTTCAATTTTTTTTCTTTCATCTTCCTTCTGTGAGAAAAAGACCATTCCTACCCCGTAACGTTCTTTTTCTGGCAGATTGATGTTTTTGCACTCTTTTCTAAAGAAAGCATCa                                                                                                       >  7:28264/1‑126 (MQ=255)
                                   gCATTGATTTGCTTTTCAATTTTTTTTCTTTCATCTTCCTTCTGTGAGAAAAAGACCATTCCTACCCCGTAACGTTCTTTTTCTGGCAGATTGATGTTTTTGCACTCTTTTCTAAAGAAAGCATCAGGGATTTGAACCa                                                                                          >  6:151838/1‑139 (MQ=255)
                                      ttGATTTGCTTTTCAATTTTTTTTCTTTCATCTTCCTTCTGTGAGAAAAAGACCATTCCTACCCCGTAACGTTCTTTTTCTGGCAGATTGATGTTTTTGCACTCTTTTCTAAAGAAAGCATCAGGGATTTGAACCAGt                                                                                        >  6:72226/1‑138 (MQ=255)
                                            tGCTTTTCAATTTTTTTTCTTTCATCTTCCTTCTGTGAGAAAAAGACCATTCCTACCCCGTAACGTTCTTTTTCTGGCAGATTGATGTTTTTGCACTCTTTTCTAAAGAAAGCATCAGGGATTTGACCCAGTAAGCCgg                                                                                 >  4:259338/1‑139 (MQ=255)
                                            tGCTTTTCAATTTTTTTTCTTTCATCTTCCTTCTGTGAGAAAAAGACCATTCCTACCCCGTAACGTTCTTTTTCTGGCAGATTGATGTTTTTGCACTCTTTTCTAAAGAAAGCATCAGGGATTTGAACCAGTAAGCCgg                                                                                 >  6:172558/1‑139 (MQ=255)
                                              cTTTTCAATTTTTTTTCTTTCATCTTCCTTCTGTGAGAAAAAGACCATTCCTACCCCGTAACGTTCTTTTTCTGGCAGATTGATGTTTTTGCACTCTTTTCTAAAGAAAGCATCAGGGATTTGAACCAGTAAGCCGGCt                                                                               <  7:151780/139‑1 (MQ=255)
                                                   cAATTTTTTTTCTTTCATCTTCCTTCTGTGAGAAAAAGACCATTCCTACCCCGTAACGTTCTTTTTCTGGCAGATTGATGTTTTTGCACTCTTTTCTAAAGAAAGCATCAGGGATTTGAACCAGTAAGCCGGCTCCGtc                                                                          >  7:93133/1‑139 (MQ=255)
                                                   cAATTTTTTTTCTTTCATCTTCCTTCTGTGAGAAAAAGACCATTCCTACCCCGTAACGTTCTTTTTCTGGCAGATTGATGTTTTTGCACTCTTTTCTAAAGAAAGCATCAGGGATTTGAACCAGTAAGCCGGCTCCGtc                                                                          >  2:166933/1‑139 (MQ=255)
                                                   cAATTTTTTTTCTTTCATCTTCCTTCTGTGAGAAAAAGACCATTCCTACCCCGTAACGTTCTTTTTCTGGCAGATTGATGTTTTTGCACTCTTTTCTAAAGAAAGCATCAGGGATTTGAACCAGTAAGCCGGCTCCGt                                                                           <  3:118927/138‑1 (MQ=255)
                                                   cAATTTTTTTTCTTTCATCTTCCTTCTGTGAGAAAAAGACCATTCCTACCCCGTAACGTTCTTTTTCTGGCAGATTGATGTTTTTGCACTCTTTTCTAAAGAAAGCATCAGGGATTTGAACCAGTAAGCCGGCTCCGt                                                                           <  1:8912/138‑1 (MQ=255)
                                                        ttttttCTTTCATCTTCCTTCTGTGAGAAAAAGACCATTCCTACCCCGTAACGTTCTTTTTCTGGCAGATTGATGTTTTTGCACTCTTTTCTAAAGAAAGCATCAGGGATTTGAACCAGTAAGCCGGCTCCGTCTCCt                                                                      <  6:51379/138‑1 (MQ=255)
                                                        ttttttCTTTCATCTTCCTTCTGTGAGAAAAAGACCATTCCTACCCCGTAACGTTCTTTTTCTGGCAGATTGATGTTTTTGCACTCTTTTCTAAAGAAAGCATCAGGGATTTGAACCAGTAAGCCGGCTCCGTCTCCt                                                                      <  8:97592/138‑1 (MQ=255)
                                                        ttttttCTTTCATCTTCCTTCTGTGAGAAAAAGACCATTCCTACCCCGTAACGTTCTTTTTCTGGCAGATTGATGTTTTTGCACTCTTTTCTAAAGAAAGCATCAGGGATTTGAACCAGTAAGCCGGCTCCGTCTCCt                                                                      <  2:19534/138‑1 (MQ=255)
                                                         tttttCTTTCATCTTCCTTCTGTGAGAAAAAGACCATTCCTACCCCGTAACGTTCTTTTTCTGGCAGATTGATGTTTTTGCACTCTTTTCTAAAGAAAGCATCAGGGAt                                                                                                  <  5:217639/109‑1 (MQ=255)
                                                         tttttCTTTCATCTTCCTTCTGTGAGAAAAAGACCATTCCTACCCCGTAACGTTCTTTTTCTGGCAGATTGATGTTTTTGCACTCTTTTCTAAAGAAAGCATCAGGGAt                                                                                                  >  6:217639/1‑109 (MQ=255)
                                                                 tcATCTTCCTTCTGTGAGAAAAAGACCATTCCTACCCCGTAACGTTCTTTTTCTGGCAGATTGATGTTTTTGCACTCTTTTCTAAAGAAAGCATCAGGGATTTGAACCAGTAAGCCGGCTCCGTCTCCTGTATCCg                                                               <  1:166933/136‑1 (MQ=255)
                                                                   aTCTTCCTTCTGTGAGAAAAAGACCATTCCTACCCCGTAACGTTCTTTTTCTGGCAGATTGATGTTTTTGCACTCTTTTCTAAAGAAAGCATCAGGGATTTGAACCAGTAAGCCGGCTCCGTCTCCTGTATCCGGATCa                                                          <  5:172558/139‑1 (MQ=255)
                                                                   aTCTTCCTTCTGTGAGAAAAAGACCATTCCTACCCCGTAACGTTCTTTTTCTGGCAGATAGATGTTTTTGCACTCTTTTCTAAAGAAAGCATCAGGGATTTGAACCAGTAAGCCGGCTCCGTCTCCTGTATCCGGATCa                                                          <  7:251179/139‑1 (MQ=255)
                                                                            cTGTGAGAAAAAGACCATTCCTACCCCGTAACGTTCTTTTTCTGGCAGATTGATGTTTTTGCACTCTTTTCTAAAGAAAGCATCAGGGATTTGAACCAGTAAGCCGGCTCCGTCTCCTGTATCCGGATCACTGCCTTg                                                  <  6:105600/138‑1 (MQ=255)
                                                                               tgAGAAAAAGACCATTCCTACCCCGTAACGTTCTTTTTCTGGCAGATTGATGTTTTTGCACTCTTTTCTAAAGAAAGCATCAGAGATTTGAACCAGTAAGCCGGCTCCGTCTCCTGTATCCGGATCACTGCCTTGcc                                                >  6:213307/1‑137 (MQ=255)
                                                                               tgAGAAAAAGACCATTCCTACCCCGTAACGTTCTTTTTCTGGCAGATTGATGTTTTTGCACTCTTTTCTAAAGAAAGCATCAGAGATTTGAACCAGTAAGCCGGCTCCGTCTCCTGTATCCGGATCACTGCCTTGcc                                                <  5:213307/137‑1 (MQ=255)
                                                                               tgAGAAAAAGACCATTCCTACCCCGTAACGGTCTTTTTGTGGCAGATTGAGGTTTTTGCCCTCTATTCTAACGAAAGCATCAGGGAATTGAACAAGTAACCCGGCTCCGTCCCCTGTATCCGGATCACTGCCTTGcc                                                <  7:266547/137‑1 (MQ=255)
                                                                                     aaaGACCATTCCTACCCCGTAACGTTCTTTTTCTGGCAGATTGATGTTTTTGCACTCTTTTCTAAAGAAAGCATCAGGGATTTGAACCAGTAAGCCGGCTCCGTCTCCTGTATCCGGATCACTGCCTTGCCCTCCTCTa                                        <  8:12003/139‑1 (MQ=255)
                                                                                            aTTCCTACCCCGTAACGTTCTTTTTCTGGCAGATTGATGTTTTTGCACTCTTTTCTAAAGAAAGCATCAGGGATTTGAACCAGTAAGCCGGCTCCGTCTCCTGTATCCGGAt                                                            >  1:237820/1‑112 (MQ=255)
                                                                                            aTTCCTACCCCGTAACGTTCTTTTTCTGGCAGATTGATGTTTTTGCACTCTTTTCTAAAGAAAGCATCAGGGATTTGAACCAGTAAGCCGGCTCCGTCTCCTGTATCCGGAt                                                            <  2:237820/112‑1 (MQ=255)
                                                                                                      cGTAACGTTCTTTTTCTGGCAGATTGATGTTTTTGCACTCTTTTCTAAAGAAAGCATCAGGGATTTGAACCAGTAAGCCGGCTCCGTCTCCTGTATCCGGATCACTGCCTTGCCCTCCTCTATGGTCTAGCTGGCAAAg                       <  8:190212/139‑1 (MQ=255)
                                                                                                      cGTAACGTTCTTTTTCTGGCAGATTGATGTTTTTGCACTCTTTTCTAAAGAAAGCATCAGGGATTTGAACCAGTAAGCCGGCTCCGTCTCCTGTATCCGGATCACTGCCTTGCCCTCCTCTATGGTCTAGCTGGCAAAg                       <  7:64677/139‑1 (MQ=255)
                                                                                                      cGTAACGTTCTTTTTCTGGCAGATTGATGTTTTTGCACTCTTTTCTAAAGAAAGCATCAGGGATTTGAACCAGTAAGCCGGCTCCGTCTCCTGTATCCGGATCACTGCCTTGCCCTCCTCTATGGTCTAGCTGGCAAAg                       <  4:49210/139‑1 (MQ=255)
                                                                                                        tAACGTTCTTTTTCTGGCAGATTGATGTTTTTGCACTCTTTTCTAAAGAAAGCATCAGGGATTTGAAcc                                                                                           >  5:166258/1‑69 (MQ=255)
                                                                                                        tAACGTTCTTTTTCTGGCAGATTGATGTTTTTGCACTCTTTTCTAAAGAAAGCATCAGGGATTTGAAcc                                                                                           <  6:166258/69‑1 (MQ=255)
                                                                                                        tAACGTTCTTTTTCTGGCAGATTGATGTTTTTGCACTCTTTTCTAAAGAAAGCATCAGGGATTTGAACCAGTAAGCCGGCTCCGTCTCCTGTATCCGGATCACTGCCTTGCCCTCCTCTATGGTCTAGCTGGCAAAg                       >  8:53813/1‑137 (MQ=255)
                                                                                                        tAACGTTCTTTTTCTGGCAGATTGATGTTTTTGCACTCTTTTCTAAAGAAAGCATCAGGGATTTGAACCAGTAAGCCGGCTCCGTCTCCTGTATCCGGATCACTGCCTTGCCCTCCTCTATGGTCTAGCTGGCAAAg                       <  7:53813/137‑1 (MQ=255)
                                                                                                        tAACGTTCTTTTTCTGGCAGATTGATGTTTTTGCACTCTTTTCTAAAGAAAGCATCAGGGATTTGAACCAGTAAGCCGGCTCCGTCTCCTGTATCCGGATCACTGCCTTGCCCTCCTCTATGGTCTAGCTGGCAAAGc                      >  6:17553/1‑138 (MQ=255)
                                                                                                        tAACGTTCTTTTTCTGGCAGATTGATGTTTTTGCACTCTTTTCTAAAGAAAGCATCAGGGATTTGAACCAGTAAGCCGGCTCCGTCTCCTGTATCCGGATCACTGCCTTGCCCTCCTCTATGGTCTAGCTGGCAAAGCa                     >  4:97991/1‑139 (MQ=255)
                                                                                                        tAACGTTCTTTTTCTGGCAGATTGATGTTTTTGCACTCTTTTCTAAAGAAAGCATCAGGGATTTGAACCAGTAAGCCGGCTCCGTCTCCTGTATCCGGATCACTGCCTTGCCCGCCTCTATGGTCTAGCTGGCAAAg                       >  6:151799/1‑137 (MQ=255)
                                                                                                            gTTCTTTTTCTGGCAGATTGATGTTTTTGCACTCTTTTCTAAAGAAAGCATCAGGGATTTGAACCAGTAAGCCGGCTCCGTCTCCTGTATCCGGATCACTGCCTTGCCCTCCTCTATGGTCTAGCTGGCAAAGCATCtt                 <  5:151838/139‑1 (MQ=255)
                                                                                                            gTTCTTTTTCTGGCAGATTGATGTTTTTGCACTCTTTTCTAAAGAAAGCATCAGGGATTTGAACCAGTAAGCCGGCTCCGTCTCCTGTATCCAGATCACTGCCTTGCCCTCCTCTATGGTCTAGCTGGCAAAGCATCtt                 <  5:276521/139‑1 (MQ=255)
                                                                                                                   ttCTGGCAGATTGATGTTTTTGCACTCTTTTCTAAAGAAAGCATCAGGGATTTGAACCAGTAAGCCGGCTCCGTCTCCTGTATCCGGATCACTGCCTTGCCCTCCTCTATGGTCTAGCTGGCAAAGCATCTTAAGTcc           <  8:93133/138‑1 (MQ=255)
                                                                                                                   ttCTGGCAGATTGATGTTTTTGCACTCTTTTCTAAAGAAAGCATCAGGGATTTGAACCAGTAAGCCGGCTCCGTCTCCTGTATCCGGATCACTGCCTTGCCCTCCTCTATGGTCTAGCTGGCAAAGCATCTTAAGTcc           <  6:252027/138‑1 (MQ=255)
                                                                                                                        gCAGATTGATGTTTTTGCACTCTTTTCTAAAGAAAGCATCAGGGATTTGAACCAg                                                                                         >  5:100494/1‑55 (MQ=255)
                                                                                                                        gCAGATTGATGTTTTTGCACTCTTTTCTAAAGAAAGCATCAGGGATTTGAACCAg                                                                                         <  6:100494/55‑1 (MQ=255)
                                                                                                                            aTTGATGTTTTTGCACTCTTTTCTAAAGAAAGCATCAGGGATTTGAACCAGTAAGCCGGCTCCGTCTCCTGTATCCGGATCACTGCCTTGCCCTCCTCTATGGTCTAGCTGGCAAAGCATCTTAAGTCCTTGTTTGAc  >  3:96646/1‑138 (MQ=255)
                                                                                                                                         |                                                                                                                            
ATCCAAGAACGACTTGGCCTTCTTGTTCAATTAGTGCATTGATTTGCTTTTCAATTTTTTTTCTTTCATCTTCCTTCTGTGAGAAAAAGACCATTCCTACCCCGTAACGTTCTTTTTCTGGCAGATTGATGTTTTTGCACTCTTTTCTAAAGAAAGCATCAGGGATTTGAACCAGTAAGCCGGCTCCGTCTCCTGTATCCGGATCACTGCCTTGCCCTCCTCTATGGTCTAGCTGGCAAAGCATCTTAAGTCCTTGTTTGAC  >  CP006881/1966512‑1966773

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑