Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 2,025,287 T→G 7.3% T175T (ACA→ACC)  U712_10010 ← UPF0720 protein yobL

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,025,2870TG 7.3% 143.4 / 5.9 55T175T (ACA→ACC) U712_10010UPF0720 protein yobL
Reads supporting (aligned to +/- strand):  ref base T (26/24);  new base G (2/2);  total (29/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.99e-01

TCGCCTTTTTTCTTATGTATGTCACTTTCTCTGTACGCTTTAGCGTTATAGTGAATTGGTGTAGCGTTTTTTCCTTTGCCTGTTGATTCCTGCAGCTTTTTAAAGTCTT‑GCTGAATAAATTGTTCGTTTGGTTCTGTTTCAGCGTATTCTGTTTTTAAATCCTCATCAAGCTTGTTTAGCTTATCGATTGTTTTTTCACGTTTGTCATCTGCAGAAGAAAGTTTGTCTTTGAAGTCTTCTGTTGAGAATATTTCAAGGGGAAGGATATCAT  >  CP006881/2025153‑2025423
                                                                                                                                       |                                                                                                                                        
tCGCCTTTTTTCTTATGTATGTCACTTTCTCTGTACGCTTTAGCGTTATAGTGAATTGGTGTAGCGTTTTTTCCTTTGCCTGTTGATTCCTGCAGCTTTTTAAAGTCTT‑GCTGAATAAATTGTTCGTTTGGTTCTGtt                                                                                                                                       <  8:134978/138‑1 (MQ=255)
   ccTTTTTTCTTATGTATGTCACTTTCTCTGTACGCTTTAGCGTTATAGTGAATTGGTGTAGCGTTTTTTCCTTTGCCTGTTGATTCCTGCAGCTTTTTAAAGTCTT‑GCTGAATAAATTGTTCGTTTGGTTCTGTTTCAg                                                                                                                                   >  6:286845/1‑139 (MQ=255)
        tttCTTATGTATGTCACTTTCTCTGTACGCTTTAGCGTTATAGTGAATTGGTGTAGCGTGTTTTCCTTTGCCTGTTGATTCCTGCAGCTTTTTAAAGTCTT‑GCTGAATAAATTGTTCGTTTGGTTCTGTTTCAGCGTa                                                                                                                               <  4:256535/138‑1 (MQ=255)
            ttATGTATGTCACTTTCTCTGTACGCTTTAGCGTTATAGTGAATTGGTGTAGCGTTTTTTCCTTTGCCTGTTGATTCCTGCAGCTTTTTAAAGTCTT‑GCTGAATAAATTGTTCGTTTGGTTCTGTTTCAGCGTATTCTg                                                                                                                          <  6:149179/139‑1 (MQ=255)
              atgtatgtCACTTTCTCTGTACGCTTTAGCGTTATAGTGAATTGGTGTAGCGTTTTTTCCTTTGCCTGTTGATTCCTGCAGCTTTTTAAAGTCTT‑GCTGAATAAATTGTTCGTTTGGTTCTGTTTCAGCGTATTCt                                                                                                                           <  1:64472/136‑1 (MQ=255)
                   tgtCACTTTCTCTGTACGCTTTAGCGTTATAGTGAATTGGTGTAGCGATTTTTCCTTTGCCTGTTGATTACTGCAGCTTTTTAAAGTATT‑GCTGAATAAATTGTTCGTTTGGTTCTTTTTAAGCGTATTCTGTTTTTa                                                                                                                    >  8:228070/1‑138 (MQ=255)
                         tttCTCTGTACGCTTTAGCGTTATAGTGAATTGGTGTAGCGTTTTTTCCTTTGCCTGTTGATTCCTGCAGCTTTTTAAAGTCTT‑GCTGAATAAATTGTTCGTTTGGTTCAGTTTCAGCGTATTCTGTTTTTAAATCCt                                                                                                              >  3:123016/1‑138 (MQ=255)
                           tctcTGTACGCTTTAGCGTTATAGTGAATTGGTGTAGCGTTTTTTCCTTTGCCTGTTGATTCCTGCAGCTTTTTAAAGTCTT‑GCTGAATAAATTGTTCGTTTGGTTCTGTTTCAGCGTAtt                                                                                                                             >  1:246961/1‑121 (MQ=255)
                           tctcTGTACGCTTTAGCGTTATAGTGAATTGGTGTAGCGTTTTTTCCTTTGCCTGTTGATTCCTGCAGCTTTTTAAAGTCTT‑GCTGAATAAATTGTTCGTTTGGTTCTGTTTCAGCGTAtt                                                                                                                             <  2:246961/121‑1 (MQ=255)
                            ctctGTACGCTTTAGCGTTATAGTGAATTGGTGTAGCGTTTTTTCCTTTGCCTGTTGATTCCTGCAGCTTTTTAAAGTCTT‑GCTGAATTAATTGTTCGTTTGGTTCTGTTTCAGCGTATTCTGTTTTTAAATCCtcat                                                                                                           <  4:125551/138‑1 (MQ=255)
                             tctGTACGCTTTAGCGTTATAGTGAATTGGTGTAGCGTTTTTTCCTTTGCCTGTTGATTCCTGCAGCGTTTTAAAGTCTT‑GCTGAAAAAATTTTTCGGTTGGTTGTGTTTCAGCGTATTCTGGTTTTAAATCCtcatc                                                                                                          >  6:273283/1‑138 (MQ=255)
                                 tACGCTTTAGCGTTATAGTGAATTGGTGTAGCGTTTTTTCCTTTGCCTGTTGATTCCTGCAGCTTTTTAAAGTCTT‑GCTGAATAAATTGTTCGTTTGGTTCTGTTTCAGCGTATTCTGTTTTTAAATCCTCATCaa                                                                                                        <  3:87294/136‑1 (MQ=255)
                                 tACGCTTTAGCGTTATAGTGAATTGGTGTAGCGTTTTTTCCTTTGCCTGTTGATTCCTGCAGCTTTTTAAAGTCTT‑GCTGAATAAATTGTTCGTTTGGTTCTGTTTCAGCGTATTCTGTTTTTAAATCCTCATCAAg                                                                                                       <  5:101653/137‑1 (MQ=255)
                                         aGCGTTATAGTGAATTGGTGTAGCGTTTTTTCCTTTGCCTGTTGATTCCTGCAGCTTTTTAAAGTCTT‑GCTGAATAAATTGTTCGTTTGGTTCTGTTTCAGCGTATTCTGTTTTTAAATCCTCATCAAGCTTGTTTAGc                                                                                             >  1:13385/1‑139 (MQ=255)
                                         aGCGTTATAGTGAATTGGTGTAGCGTTTTTTCCTTTGCCTGTTGATTCCTGCAGCTTTTTAAAGTCTT‑GCTGAATAAATTGTTCGTTTGGTTCTGTTTCAGCGTATTCTGTTTTTAAATCCTCATCAAGCTTGTTTAGc                                                                                             >  3:98683/1‑139 (MQ=255)
                                         aGCGTTATAGTGAATTGGTGTAGCGTTTTTTACGTTTCCTGTTTATTACAGGAAGATTTTTAAGTATTAATTTAAAAAATTTTTC‑TTTTGATCTGTTTCAACCTATTATTTTATTAAATACGAAACAAACTTGCTTat                                                                                              >  2:243608/1‑137 (MQ=255)
                                                taGTGAATTGGTGTAGCGTTTTTTCCTTTGCCTGTTGATTCCTGCAGCTTTTTAAAGTCTT‑GCTGAATAAATTGTTCGTTTGGTTCTGTTTCAGCGTATTCTGTTTTTAAATCCTCATCAAGCTTGTTTAGCTTATCGa                                                                                      <  3:115854/139‑1 (MQ=255)
                                                taGTGAATTGGTGTAGCGTTTTTTCCTTTGCCTGTTGATTCCTGCAGCTTTTTAAAGTCTT‑GCTGAATAAATTGTTCGTTTGGTTCTGTTTCAGCGTATTCTGTTTTTAAATCCTCATCAAGCTTGTTTAGCTTATCGa                                                                                      >  3:64792/1‑139 (MQ=255)
                                                taGTGAATTGGTGTAGCGTTTTTTCCTTTGCCTGTTGATTCCTGCAGCTTTTTAAAGTCTT‑GCTGAATAAATTGTTCGTTTGGTTCTGTTTCAGCGTATTCTGTTTTTAAATCCTCATCAAGCTTGTTTAGCTTATCGa                                                                                      >  4:285546/1‑139 (MQ=255)
                                                taGTGAATTGGTGTAGCGGTTTTTGCTTTTCCGGTTGTTTACGGCCGCGTTTTTAAGTGTT‑TGTGTATAAATTGTTGGTTTTGTTGTGGTTTGGGGTTTTGTGTTTTTTAATGCTCATTAAGCTTGTTTAGGTTTTc                                                                                        >  2:284317/1‑137 (MQ=255)
                                                taGTGAATTGGTGTAGCGGTTTTTCCTTTGCCTGTTGATTGCTGCAGCTTTTTAAAGTATT‑GCGGAATAAATTGTTCGTTTGGTTGTGTTTGAGCGTATTATGTTTTTAAATCCTCATCAAGCTTGTTTAGCttt                                                                                          >  5:97561/1‑134 (MQ=255)
                                                       ttGGTGTAGCGTTTTTTCCGTTGCCTGTTGATTGCAGCAGCGTTTTAAAGTCTT‑GCTGAATAAATTGTTCGTTTGGTTCTGTTTCAGCGTATTATGTTTTTAAATCCTCATCAAGGTTGTTTAGGATATCGATTGtttt                                                                               >  4:170187/1‑139 (MQ=255)
                                                        tGGTGTAGCGTTTTTTCCTTTGCCTGTTGTTTCCTGCAGCTTTTTAAAGTCTT‑GCTGAATAAATTGTTCGTTTGGTTCGGtt                                                                                                                                       >  7:120402/1‑82 (MQ=255)
                                                        tGGTGTAGCGTTTTTTCCTTTGCCTGTTGATTCCTGCAGCTTTTTAAAGTCTT‑GCTGAATAAATTGTTCGTTTGGTTCGGtt                                                                                                                                       <  8:124287/82‑1 (MQ=255)
                                                        tGGTGTAGCGTTTTTTCCTTTGCCTGTTGATTCCTGCAGCTTTTTAAAGTCTT‑GCTGAATAAATTGTTCGTTTGGTTCGGtt                                                                                                                                       >  7:124287/1‑82 (MQ=255)
                                                        tGGTGTAGCGTTTTTTCCTTTGCCTGTTGATTCCTGCAGCTTTTTAAAGTCTT‑GCTGAATAAATTGTTCGTTTGGTTCGGtt                                                                                                                                       <  8:120402/82‑1 (MQ=255)
                                                          gtgtAGCGTTTTTTCCTTTGCCTGTTGATTCCTGCAGCTTTTTAAAGTCTT‑GCTGAATAAATTGTTAGTTTGGTTCTGTTTCAGCGTATTCTGTTTTTAAATCCTCAAAAAGCTTGTTTAGCTTATCGATTGtttttt                                                                             >  7:42494/1‑138 (MQ=255)
                                                             tAGCGTTTTTTCCTTTGCCTGTTGATTCCTGCAGCATTTTAAAGTATT‑GATTAAAAAATTTTTCGTTTGGTTATGTTTCAGCGTATTATGTTTTTAAATACTCATAAAGCTTGGTTAGCTTATCGATTGTTTTTTCACg                                                                         >  6:125378/1‑139 (MQ=255)
                                                               gCGTTTTTTACTTTGCCTGTTGATTCCTGCAGCTTTTTAAAGTCTT‑GCTGAATAAATTGTTCGTTTGGTTCTGTTTCAGCGTATTCTGTTTTTAAATCCTCATCAAGCTTGTTTAGCTTATCGATTGTTTTTTCACGt                                                                        <  1:228203/138‑1 (MQ=255)
                                                                cGTTTTTTACTTTGCCTGTTGATTCCTGCAGCTTTTTAAAGTCTT‑GCTGAATAAATTGTTAGTTTGTTTCTGTTTGAGCGTATTCTGTTTTTAAATCCTCATCAATCTTGTTTAGGTTATAGATTGTTTTTTAACGttt                                                                      >  8:42846/1‑139 (MQ=255)
                                                                     tttCCTTTGCCTGTTGATTCCTGCAGCTTTTTAAAGTCTT‑GCTGAATAAATTGTTCGTTTGGTTCTGTTTCAGCGTATTCTGTTTTTAAATCCTCATCAAGCTTGTTTAGCTTATCGATTGTTTTTTCACGTTTGTCa                                                                  >  7:117608/1‑138 (MQ=255)
                                                                         cTTTGCCTGTTGATTCCTGCAGCTTTTTAAAGTCTT‑GCTGAATAAATTGTTCGTTTGGTTCTGTTTCAGCGTATTCTGTTTTTAAATCCTCATCAAGCTTGTTTAGCTTATCGATTGTTTTTTCACGTTTGTCATCTg                                                              <  7:42846/138‑1 (MQ=255)
                                                                                     aTTCCTGCAGCTTTTTAAAGTCTT‑GCTGAATAAATTGTTCGTTTGGTTCTGTTTCAGCGTATTCTGTTTTTAAATCCTCATCAAGCTCGTTTAGCTTATCGATTGTTTTTTCACGTTTGTCATCTGCAGAAGAAAGttt                                                 <  2:213339/139‑1 (MQ=255)
                                                                                       tCCTGCAGCTTTTTAAAGTCTT‑GCTGAATAAATTGTTCGTTTGGTTCTGTTTCAGCGTATTCTGTTTTTAAATCCTCATCAAGCTTGTTTAGCTTATCGATTGTTTTTTCACGTTTGTCATCTg                                                              <  1:227247/124‑1 (MQ=255)
                                                                                       tCCTGCAGCTTTTTAAAGTCTT‑GCTGAATAAATTGTTCGTTTGGTTCTGTTTCAGCGTATTCTGTTTTTAAATCCTCATCAAGCTTGTTTAGCTTATCGATTGTTTTTTCACGTTTGTCATCTg                                                              >  2:227247/1‑124 (MQ=255)
                                                                                              gCTTTTTAAAGTCTT‑GCTGAATAAATTGTTCGTTTGGGTCTGTTTCAGCGTATTCTGTTTTTAAATCCTCATCAAGCTTGTTTAGCTTATCGATTGTTTTTTCACGTTTGTCATCTGCAGAAGAAAGTTTGTCTTTGaa                                        <  5:273283/139‑1 (MQ=255)
                                                                                                  tttAAAGTCTT‑GCTGAATAAATTGTTCGTTTGGTTCTGTTTCAGCGTATTCTGTTTTTAAATCCTCATCAAGCTTGTTTAGCTTATCGATTGTTTTTTCACGTTTGTCATCTGCAGAAGAAAGTTTGTCTTTGAAGtct                                    >  6:140308/1‑139 (MQ=255)
                                                                                                    tAAAGTCTT‑GCTGAATAAATTGTTCGTTTGGTTCTGTTTCAGCGTATTCTGTTTTTAAATCCTCATCAAGCTTGTTTAGCTTATCGATTGTTTTTTCACGTTTGTCATCTGCAGAAGAAAGTTTGTCTTTGAAGtctt                                   <  6:275298/138‑1 (MQ=255)
                                                                                                        gTCTT‑GCTGAATAAATTGTTCGTTTGGTTCTGTTTCAGCGTATTCTGTTTTTAAATCCTCATCAAGCTTGTTTAGCTTATCGATTGTTTTTTCACGTTTGTCATCTGCAGAAGAAAGTTTGTCTTTGAAGTCTTCTGtt                              <  8:66866/139‑1 (MQ=255)
                                                                                                            t‑GCTGAATAAATTGTTCGTTTGGTTCTGTTTCAGCGTATTCTGTTTTTAAATCCtcat                                                                                                           >  7:11045/1‑58 (MQ=255)
                                                                                                            t‑GCTGAATAAATTGTTCGTTTGGTTCTGTTTCAGCGTATTCTGTTTTTAAATCCtcat                                                                                                           <  8:11045/58‑1 (MQ=255)
                                                                                                            t‑GCTGAATAAATTGTTCGTTTGGTTCTGTTTCAGCGTATTCTGTTTTTAAATCCTCATCAAGCTTGTTTAGCTTATCGATTGTTTTTTCACGTTTGTCATCTGCAGAAGAAAGTTTGTCTTTGAAGTCTTCTGTTgag                           >  7:194502/1‑138 (MQ=255)
                                                                                                              gCTGAATAAATTGTTCGTTTGGTTCTGTTTCAGCGTATTCTGTTTTTAAATCCTCATCAAGCTTGTTTAGCTTATCGATTGTTTTTTCACGTTTGTCATCTGCAGAAGAAAGTTTGTCTTTGAAGTCTTCTGTTgaga                          <  5:286845/138‑1 (MQ=255)
                                                                                                               cTGAATAAATTGTTCGTTTGGTTCTGTTTCAGCGTATTCTGTTTTTAAATCCTCATCAAGCTTGTTTAGCTTATCGATTGTTTTTTCACGTTTGTCATCTGCAGAAGAAAGTTTGTCTTTGAAGTCTTCTGTTGAGaa                         <  8:117608/138‑1 (MQ=255)
                                                                                                                    tAAATTGTTCGTTTGGTTCTGTTTCAGCGTATTCTGTTTTTAAATCCTCATCAAGCTTGTTTAGCGTATCGATTGTTTTTTCACGTTTGTCATCTGCAGAAGAAAGTTTGTCTTTGAAGTCTTCTGTTGAGAATAttt                    >  6:180745/1‑138 (MQ=255)
                                                                                                                    tAAATTGTTCGTTTGGTTCTGTTTCAGCGTATTCTGTTTTTAAATCCTCATCAAGCTTGTTTAGCGTATCGATTGTTTTTTAACGTTTGTCATCTGCAGAAGAAAGTTTGTCTTTGAAGTCTTCGGTTGAGAATAttt                    >  6:184496/1‑138 (MQ=255)
                                                                                                                      aaTTGTTCGTTTGGTTCTGTTTCAGCGTATTCTGTTTTTAAATCCTCATCAAGCTTGTTTAGCTTATCGATTGTTTTTTCACGTTTGTCATCTGCAGAAGAAAGTTTGTCTTTGAAGTCTTCTGTTGAGAATATTTCa                  <  8:42494/138‑1 (MQ=255)
                                                                                                                       aTTGTTCGTTTGGTTCTGTTTCAGCGTATTCTGTTTTTAAATCCTCATCAAGCTTGTTTAGCTTATCGATTGTTTTTTCACGTTTGTCATCTGCAGAAGAAAGTTTGTCTTTGAAGTCTTCTGTTGAGAATATTTCAAg                >  6:71973/1‑139 (MQ=255)
                                                                                                                            tCGTTTGGTTCTGTTTCAGCGTATTCTGTTTTTAAATCCTCATCAAGCTTGTTTAGCTTATCGATTGTTTTTTCACGTTTGTCATCTGCAGAAGAAAGTTTGTCTTTGAAGTCTTCTGTTGAGAATATTTCAAgggg             >  5:240299/1‑137 (MQ=255)
                                                                                                                               tttGGTTCTGTTTCAGCGTATTCTGTTTTTAAATCCTCATCAAGCTTGTTTAGCTTATCGATTGTTTTTTCACGTTTGTCATCTGCAGAAGAAAGTTTGTCTTTGAAGTCTTCTGTTGAGAATATTTCAAGGGGAAGGa        <  4:250604/139‑1 (MQ=255)
                                                                                                                                ttGGTTCTGTTTCAGCGTATTCTGTTTTTAAATCCTCATCAAGCTTGTTTAGCTTATCGATTGTTTTTTCACGTTTGTCATCTGCAGAAGAAAGTTTGTCTTTGAAGTCTTCTGTTGAGAATATTTCAAGGGGAAGGa        >  1:41812/1‑138 (MQ=255)
                                                                                                                                 tGGTTCTGTTTCAGCGTATTCTGTTTTTAAATCCTCATCAAGCTTGTTTAGCTTATCGATTGTTTTTTCACGTTTGTCATCTGCAGAAGAAAGTTTGTCTTTGAAGTCTTCTGTTGAGAATATTTCaa                 <  7:201262/128‑1 (MQ=255)
                                                                                                                                 tGGTTCTGTTTCAGCGTATTCTGTTTTTAAATCCTCATCAAGCTTGTTTAGCTTATCGATTGTTTTTTCACGTTTGTCATCTGCAGAAGAAAGTTTGTCTTTGAAGTCTTCTGTTGAGAATATTTCAAGGGGAAGGat       <  1:17096/138‑1 (MQ=255)
                                                                                                                                 tGGTTCTGATTCAGCGTATTCTGTTTTTAAATCCTCATCAAGCTTGTTTAGCTTATCGATTGTTTTTTCACGTTTGTCATCTGCAGAAGAAAGTTTGTCTTTGAAGTCTTCTGTTGAGAATATTTCaa                 >  8:201262/1‑128 (MQ=255)
                                                                                                                                      cTGTTTCAGCGTATTCTGTTTTTAAATCCTCATCAAGCTTGTTTAGCTTATCGATTGTTTTTTCACGTTTGTCATCTGCAGAAGAAAGTTTGTCTTTGAAGTCTTCTGTTGAGAATATTTCAAGGGGAAGGATATCAt  <  4:98683/138‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                        
TCGCCTTTTTTCTTATGTATGTCACTTTCTCTGTACGCTTTAGCGTTATAGTGAATTGGTGTAGCGTTTTTTCCTTTGCCTGTTGATTCCTGCAGCTTTTTAAAGTCTT‑GCTGAATAAATTGTTCGTTTGGTTCTGTTTCAGCGTATTCTGTTTTTAAATCCTCATCAAGCTTGTTTAGCTTATCGATTGTTTTTTCACGTTTGTCATCTGCAGAAGAAAGTTTGTCTTTGAAGTCTTCTGTTGAGAATATTTCAAGGGGAAGGATATCAT  >  CP006881/2025153‑2025423

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑