Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 2,064,606 A→G 9.7% L468P (CTG→CCG)  U712_10215 ← Uncharacterized protein yojO

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,064,6060AG 9.7% 109.5 / 4.6 41L468P (CTG→CCG) U712_10215Uncharacterized protein yojO
Reads supporting (aligned to +/- strand):  ref base A (21/16);  new base G (2/2);  total (23/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.76e-01

TGTCTTAATGATGATTGGAAGGGAATAACCGTATTGAAATAGTTTGGCTGGCTTTTTTCGGTTGCATCATTTGTGTCCTCCCAAAAACCGACAATCTGATGCGGAACCGCTACCGATTTCAACGCTTCGTGAAACAGCACGATTCCTCGTTTTGTTTCGTCCATTTTGTCGAACATGCTGGCGGAACAATCGACCAGCAATGTAAAAACTGCGTCGATTTCTGAAGATGGTTCTTGCTTTTTGTAGAACAGGCGCGGATTTCGCTCAGTG  >  CP006881/2064471‑2064740
                                                                                                                                       |                                                                                                                                      
tGTCTTAATGATGATTGGAAGGGAATAACCGTATTGAAATAGTTTGGCTGGCTTTTTTCGGTTGCATCATTTGTGTCCTCCCAAAAACCGACAATCTGATGCGGATCCGCTACCGATTTCAACGCTTCGTGAAACAGcc                                                                                                                                     >  1:125844/1‑138 (MQ=255)
tGTCTTAATGATGATTGGAAGGGAATAACCGTATTGAAATAGTTTGGCTGGCTTTTTTCGGTTGCATCATTTGTGTCCTCCCAAAAACCGACAATCTGATGCGGAACCGCTACCGATTTCAACGCTTCGTGAAACGGCa                                                                                                                                     >  1:156913/1‑139 (MQ=255)
tGTCTTAATGATGATTGGAAGGGAATAACCGTATTGAAATAGTTTGGCTGGCTTTTTTCGGTTGCATCATTTGTGTCCTCCCAAAAACCGACAATCTGATGCGGAACCGCTACCGATTTCAACGCTTCGTGAAACGGCa                                                                                                                                     >  1:161030/1‑139 (MQ=255)
tGTCTTAATGATGATTGGAAGGGAATAACCGTATTGAAATAGTTTGGCTGGCTTTTTTCGGTTGCATCATTTGTGTCCTCCCAAAAACCGACAATCTGATGCGGAACCGCTACCGATTTCAACGCTTCGTGAAACAGCa                                                                                                                                     >  4:147042/1‑139 (MQ=255)
  tCTTAATGATGATTGGAAGGGAATAACCGTATTGAAATAGTTTGGCTGGCTTTTTTCGGTTGCATCATTTGTGTCCTCCCAAAAACCGACAATCTGATGCGGAACCGCTACCGATTTCAACGCTTCGTGAAACAGCAc                                                                                                                                    >  8:215134/1‑138 (MQ=255)
      aaTGATGATTGGAAGGGAATAACCGTATTGAAATAGTTTGGCTGGCTTTTTTCGGTTGCATCATTTGTGTCCTCCCAAAAACCGACAATCTGATGCGGAACCGCTACCGATTTCAACGCTTCGTGAAACAGCACGAt                                                                                                                                 <  6:100808/137‑1 (MQ=255)
            gatTGGAAGGGAATAACCGTATTGAAATAGTTTGGCTGGCTTTTTTCGGTTGCATCTTTTGTGTCCTCCCAAAAACCGACAATCTGATGCGGAACCGCTACCGATTTCAACGCTTCGTGAAACAGCACGATTCCTCGta                                                                                                                         >  7:5987/1‑138 (MQ=255)
            gatTGGAAGGGAATAACCGTATTGAAATAGTTTGGCTGGCTTTTTTCGGTTGCATCATTTGTGTCCTCCCAAAAACCGACAATCTGATGCGGAACCGCTACCGATTTCAACGCTTCGTGAAACAGCACTCTTCCTCGtt                                                                                                                         >  5:40349/1‑139 (MQ=255)
                        aTAACCGTATTGAAATAGTTTGGCTGGCTTTTTTCGGTTGCATCATTTGTGTCCTCCCAAAAACCGACAATCTGATGCGGAACCGCTACCGATTTCAACGCTTCGTGAAACAGCACGATTCCTCGTTTTGTTTCGTcc                                                                                                              >  4:33615/1‑138 (MQ=255)
                            ccGTATTGAAATAGTTTGGCTGGCTTTTTTCGGTTGCATCATTTGTGTCCTCCCAAAAACCGACAATCTGATGCGGAACCGCTACCGATTTCAACGCTTCGTGAAACAGCACGATTCCTCGTTTTGTTTCGTCCAtttt                                                                                                         >  8:181353/1‑139 (MQ=255)
                                aTTGAAATAGTTTGGCTGGCTTTTTTCGGTTGCATCATTTGTGTCCTCCCAAAAACCGACAATCTGATGCGGAACCGCTACCGATTTCAACGCTTCGTGAAACAGCACGATTcc                                                                                                                              <  6:67983/114‑1 (MQ=255)
                                aTTGAAATAGTTTGGCTGGCTTTTTTCGGTTGCATCATTTGTGTCCTCCCAAAAACCGACAATCTGATGCGGAACCGCTACCGATTTCAACGCTTCGTGAAACAGCACGATTcc                                                                                                                              >  5:67983/1‑114 (MQ=255)
                                 ttGAAATAGTTTGGCTGGCTTTTTTCGGTTGCATCATTTGTGTCCTCCCAAAAACCGACAATCTGATGCGGAACCGCTACCGATTTCAACGCTTCGTGAAACAGCACGATTCCTCGTTTTGTTTCGTCCATTTTGTCg                                                                                                     >  7:96115/1‑138 (MQ=255)
                                 ttGAAATAGTTTGGCTGGCTTTTTTCGGTTGCATCATTTGTGTCCTCCCAAAAACCGACAATCTGATGCGGAACCGCTACCGATTTCAACGCTTCGTGAAACAGCACGATTCCTCGTTTTGTTTCGTCCATTTTGTCg                                                                                                     >  7:49658/1‑138 (MQ=255)
                                 ttGAAATAGTTTGGCTGGATTTTTTCGGTTGCATCATTTGTGTCCTCCCAAAAACCGACAATCTGATGCGGAACCGCTACCGATTTCAACGCTTCGTGAAACAGAACGATTCCTCGTTTTGTTTCGTCCATTTAGTcc                                                                                                     >  6:29203/1‑137 (MQ=255)
                                    aaaTAGTTTGGCTGGCTTTTTTCGGTTGCATCATTTGTGTCCTCCCAAATACCGACAATCTGATGCGGAACCGCTACCGATTTCAACGCTTCGTGAAACAGCACGATTCCTCGTTTTGTTTCGTCCATTTTGTCGAACa                                                                                                 >  5:290894/1‑139 (MQ=255)
                                         gTTTGGCTGGCTTTTTTCGGTTGCATCATTTGTGTCCTCCCAAAAACCGACAATCTGATGCGGAACCGCTACCGATTTCAACGCTTCGTGAAACAGCACGAGTCCTCGTTTTGTTTCGTCCATTTTGTCTAACATGCTg                                                                                            >  4:99110/1‑139 (MQ=255)
                                          tttGGCTGGCTTTTTTCGGTTGCATCATTTGTGTCCTCCCAAAAACCGACAATCTGATGCGGAACCGCTACCGATTTCAACGCTTCGTGAAACAGCACGATTCCTCGTTTTGTTTCGTCCATTTTGTCGAACATGCTgg                                                                                           >  4:237059/1‑139 (MQ=255)
                                                  gctTTTTTCGGTTGCATCATTTGTGTCCTCCCAAAAACCGACAATCTGATGCGGAACCGCTACCGATTTCAACGCTTCGTGAAACAGCACGATTCCTCGTTTTGTTTCGTCCATTTTGTCGAACATGCTGGCGGAACaa                                                                                   <  2:162519/139‑1 (MQ=255)
                                                         tCGGTTGCATCATTTGTGTCCTCCCAAAAACCGACAATCTGATGCGGAACCGCTACCGATTTCAACGCTTCGTGAAACAGCACGATTCCTCGTTTTGTTTCGTCCATTTTGTCGAACATGCTGGCGGAACAATCGAcc                                                                             <  3:147042/138‑1 (MQ=255)
                                                           ggTTGCATCATTTGTGTCCTCCCAAACACCGACAATCTGATGCGGCACCGCTACCGAATGCATCGCTTCGTGAAACAGCACGATTCCTCGTTTTCTTTCGTCCATTTTGTCGAACATGCTGGCGGAACAATCGACCAGc                                                                          <  3:99110/139‑1 (MQ=255)
                                                           ggTTGCATCATTTGTGTCCTCCCAAAAACCGACAATCTGATGCGGAACCGCTACCGATTTCAACGCTTCGTGAAACAGCACGATTCCTCGTTTTGTTTCGTCCATTTTGTCGAACATGCTGGCGGAACAATCGACCAGc                                                                          <  3:141139/139‑1 (MQ=255)
                                                           ggTTGCATCATTTGTGTCCTCCCAAAAACCGACAATCTGATGCGGAACCGCTACCGATTTCAACGCTTCGTGAAACAGCACGATTCCTCGTTTTGTTTCGTCCATTTTGTCGAACATGCTGGCGGAACAATCGACCAGc                                                                          <  6:103743/139‑1 (MQ=255)
                                                                             cacccAAAAACCGACAATCTGATGCGGAACCGCTACCGATTTCAACGCTTCGGGAAACAGCACGATTCCTCGTTTTGTTTCGTCCATTTTGTCGAACATGCTGGCGGAACAATCGACCAGCAATGTAAAAACTGCGTCg                                                        <  4:164955/137‑1 (MQ=255)
                                                                               cccAAAAACCGACAATCTGATGCGGAACCGCTACCGATTTCAACGCTTCGTGAAACAGCACGATTCCTCGTTTTGTTTCGTCCATTTTGTCGAACATGCTGGCGGAACAATCGACCAGCAATGTAAAAACTGCGTCGAt                                                      <  7:215134/139‑1 (MQ=255)
                                                                                ccAAAAACCGACAATCTGATGCGGAACCGCTACCGATTTCAACGCTTCGTGAAACAGCACGATTCCTCGTTTTGTTTCGTCCATTTTGTCGAACATGCTGGCGGAACAATCGACCAGCAATGTAAAAACTGCGTCGa                                                       <  7:181353/137‑1 (MQ=255)
                                                                                        cGACAATCTGATGCGGAACCGCTACCGATTTCAACGCTTCGTGAAACAGCACGATTCCTCGTTTTGTTTCGTCCATTTTGTCGAACATGCTGGCGGAACAATCGACCAGCAATGTAAAAACTGCGTCGATTTCTGAAGa                                             <  2:18147/139‑1 (MQ=255)
                                                                                          aCAATCTGATGCGGAACCGCTACCGATTTCAACGCTTCGTGAAACAGCACGATTCCTCGTTTTGTTTCGTCCATTTTGTCGAGCATGCTGGCGGAACAATCGACCAGCAATGTAAAAACTGCGTCGATTTCTGAAGATg                                           >  7:77016/1‑139 (MQ=255)
                                                                                            aaTCTGATGCGGAACCGCTACCGATTTCAACGCTTCGTGAAACAGCACGATTCCTCGTTTTGTTTCGTCCATTTTGTCGAACATGCTGGCGGAACAATCGACCAGCAATGTAAAAACTGCGTCGATTTCTGAAGATGGt                                         >  5:163239/1‑139 (MQ=255)
                                                                                                   tGCGGAACCGCTACCGATTTCAACGCTTCGTGAAACAGCACGATTCCTCGTTTTGTTTCGTCCATTTTGTCGAACATGCTGGCGGAACAATCGACCAGCAATGTAAAAACTGCGTCGATTTCTGAAGATGGTTCTTGCt                                  <  3:237059/139‑1 (MQ=255)
                                                                                                        aaCCGCTACCGATTTCAACGCTTCGTGAAACAGCACGATTCCTCGTTTTGTTTCGTCCATTTTGTCGAACATGCTGGCGGAACAATCGACCAGCAATGTAAAACCTGCGTCGATTTCGGAAGATGGTTCTTTGTTTTTg                             >  2:279913/1‑139 (MQ=255)
                                                                                                        aaCCGCTACCGATTTCAACGCTTCGTGAAACAGCACGATTCCTCGTTTTGTTTCGTCCATTTTGTCGAACATGCTGGCGGAACAATCGACCAGCAATGTAAAAACTGCGTCGATTTCTGAAGATGGTTCTTGCTTTTTg                             <  3:33615/139‑1 (MQ=255)
                                                                                                         aCCGCTACCGATTTCAACGCTTCGTGAAACAGCACGATTCCTCGTTTTGTTTCGTCCATTTTGTCGAACATGCTGGCGGAACAATCGACCAGCAATGTAAAAACTGCGTCGATTTCTGAAGa                                             <  1:281804/122‑1 (MQ=255)
                                                                                                         aCCGCTACCGATTTCAACGCTTCGTGAAACAGCACGATTCCTCGTTTTGTTTCGTCCATTTTGTCGAACATGCTGGCGGAACAATCGACCAGCAATGTAAAAACTGCGTCGATTTCTGAAGa                                             >  2:281804/1‑122 (MQ=255)
                                                                                                          ccGCTACCGATTTCAACGCTTCGTGAAACAGCACGATTCCTCGTTTTGTTTCGTCCATTTTGTCGAACATGCTGGCGGAACAATCGACCAGCAATGTAAAAACTGCGTCGATTTCTGAAGATGGTTCTTGCTTTTTg                             >  5:263481/1‑137 (MQ=255)
                                                                                                              tACCGATTTCAACGCTTCGTGAAACAGCACGATTCCTCGTTTTGTTTCGTCCATTTTGTCGAACATGCTGGCGGAACAATCGACCAGCAATGTAAAAACTGCGTCGATTTCTGAAGATGGTTCTTGCTTTTTGTAgga                        >  3:119398/1‑136 (MQ=255)
                                                                                                                        aaCGCTTCGTGAAACAGCACGATTCCTCGTTTTGTTTCGTCCATTTTGTCGAACATGCTGGCGGAACAATCGACCAGCAATGTAAAAACTGCGTCGATTTCTGAAGATGGTTCTTGCTTTTTGTAGAACAGGCGCGGAt             >  2:47628/1‑139 (MQ=255)
                                                                                                                               cttGACACAGCACGATTCCTCGTTTTGTTTCGTCCATTTTGTCGAAGATGCTGGCGGAACAATCGACCAGCAATGTAAAAACTGCGTCGATTTCTGAAGATGGTTCTTGCTTTTTGTAGAACAGGCGCGGATTTCGCt       <  5:29203/136‑1 (MQ=255)
                                                                                                                               cGTGAAACGGCACGATTCCTCGTTTTGTTTCGTCCATTTTGTCGAACATGCTGGCGGAACAATCGACCAGCAATGTAAAAACTGCGTCGATTTCTGAAGATGGTTCTTGCTTTTTGTAGAACAGGCGCGGATTTCGCt       <  2:156913/138‑1 (MQ=255)
                                                                                                                               cGTGAAACGGCACGATTCCTCGTTTTGTTTCGTCCATTTTGTCGAACATGCTGGCGGAACAATCGACCAGCAATGTAAAAACTGCGTCGATTTCTGAAGATGGTTCTTGCTTTTTGTAGAACAGGCGCGGATTTCGCt       <  2:161030/138‑1 (MQ=255)
                                                                                                                                   aaaCAGCACGATTCCTCGTTTTGTTTCGGCACTTTTGTCGAACATGCTGGCGGAACAATCGACCAGCAATGTAAAAACTGCGTCGATTTCTGAAGATGGTTCTTGCTTTTTGTAGAACAGGCGCGGATTTCGCTCAGTg  <  6:40349/139‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                      
TGTCTTAATGATGATTGGAAGGGAATAACCGTATTGAAATAGTTTGGCTGGCTTTTTTCGGTTGCATCATTTGTGTCCTCCCAAAAACCGACAATCTGATGCGGAACCGCTACCGATTTCAACGCTTCGTGAAACAGCACGATTCCTCGTTTTGTTTCGTCCATTTTGTCGAACATGCTGGCGGAACAATCGACCAGCAATGTAAAAACTGCGTCGATTTCTGAAGATGGTTCTTGCTTTTTGTAGAACAGGCGCGGATTTCGCTCAGTG  >  CP006881/2064471‑2064740

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑