Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 2,264,965 T→A 8.2% E46V (GAA→GTA)  U712_11420 ← Purine nucleoside phosphorylase 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,264,9650TA 8.2% 139.2 / 4.4 49E46V (GAA→GTA) U712_11420Purine nucleoside phosphorylase 1
Reads supporting (aligned to +/- strand):  ref base T (31/14);  new base A (2/2);  total (33/16)
Fisher's exact test for biased strand distribution p-value = 5.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.45e-01

ACTTTCTCCATTGAGTAGCCTTCATAAAAATGAAAGCGGCCCTGCATTGCAATGACGGAAACTCCTTCAAGAGTGCCAAGCACAAGCTGTCCGGCATGCCCTTCAACAGTAGATACCGGGAATTCAGGTATATCTTCATATTTCAGCTTGACCGGATTTTCGATTTCGTCCGCCAAAATGCCAAGACCTGAGCCTAAAATAAGGCCGATCTTTGGAGATTCCGGCAGGTTTTGTTTAATAAAAGCGGCTGCGCGTTCAATT  >  CP006881/2264830‑2265090
                                                                                                                                       |                                                                                                                             
aCTTTCTCCATTGAGTAGCCTTCATAAAAATGAAAGCGGCCCTGCATTGCAATGACGGAAACTCCTTCAAGAGTGCCAAGCACAAGCTGTCCGGCATGCCCTTCAACAGTAGATACCGGGAATTCAGGTATATCTTCat                                                                                                                            >  2:110033/1‑139 (MQ=255)
  tttCTCCATTGAGTAGCCTTCATAAAAATGAAAGCGGCCCTGCATTGCAATGACGGAAACTCCTTCAAGAGTGCCAAGCACAAGCTGTCCGGCATGCCCTTCAACAGTAGATACCGGGAATTCAGGTATATCTTCatat                                                                                                                          >  4:164014/1‑139 (MQ=255)
  tttCTCCATTGAGTAGCCTTCATAAAAATGAAAGCGGCCCTGCATTGCAATGACGGAAACTCCTTCAAGAGTGCCAAGCACAAGCTGTCCGGCATGCCCTTCAACAGTAGATACCGGGAATTCAGGTATATCTTCata                                                                                                                           >  3:202417/1‑138 (MQ=255)
    tctcCATTGAGTAGCCTTCATAAAAATGATCGCGGCACTGCATTGCAATGACGGATACTCCTTCAAGAGTGCCAAGCTCAAGCTGTCCGGCATGCCCTTCAACAGTAGATACCGGGACTTCAGGTGATTCTTCATAttt                                                                                                                        >  8:287382/1‑139 (MQ=255)
                    ttCATAAAAATGAAAGCGGCCCTGCATTGCAATGACGGAAACTCCTTCAAGAGTGCCAAGCACAAGCTGTCCGGCATGCCCTTCAACAGTAGATACCGGGAATTCAGGTATATCTTCATATTTCAGCTTGACCGGAgtt                                                                                                        >  5:247179/1‑139 (MQ=255)
                       aTAAAAATGAAAGCGGCCCTGCATTGCAATGACGGAAACTCCTTCAAGAGTGCCAAGCACAAGCTGTCCGGCATGCCCTTCAACAGTAGATACCGGGAATTCAGGTATATCTTCATATTTCAGCTTGACCGGATTTTc                                                                                                      >  7:232957/1‑138 (MQ=255)
                       aTAAAAATGAAAGCGGCCCTGCATTGCAATGACGGAAACTCCTTCAAGAGTGCCAAGCACAAGCTGTCCGGCATGCCCTTCAACAGTAGATACCGGGAATTCAGGTATATCTTCATATTTCAGCTTGACCGGATTTTc                                                                                                      >  7:208877/1‑138 (MQ=255)
                       aTAAAAATGAAAGCGGCCCTGCATTGCAATGACGGAAACTCCTTCAAGAGTGCCAAGCACAAGCTGTCCGGCATGCCCTTCAACAGTAGATACCGGGAATTCAGGTATATCTTCATATTTCAGCTTGACCGGATTTTc                                                                                                      >  4:119661/1‑138 (MQ=255)
                                      gCCCTGCATTGCAATGACGGAAACTCCTTCAAGAGTGCCAAGCACAAGCTGTCCGGCATGCCCTTCAACAGTAGATACCGGGAATTCAGGTATATCTTCATATTTCAGCTTGACCGGATTTTCGATTTCGTCCGCCaaa                                                                                      <  8:208877/139‑1 (MQ=255)
                                            cATTGCAATGACGGAAACTCCTTCAAGAGTGCCAAGCACAAGCTGTCCGGCATGCCCTTCAACAGTAGATACCGGGAATTCAGGTATATCTTCATATTTCAGCTTGACCGGATTTTCGATTTCGTCCGCCAAAATGcc                                                                                 <  8:4370/138‑1 (MQ=255)
                                            cATTGCAATGACGGAAACTCCTTCAAGAGTGCCAAGCACAAGCTGTCCGGCATGCCCTTCAACAGTAGATACCGGGAATTCAGGTATATCATCATATTTCAGCTTGACCGGATTTTCGATTTCGTCCGCCAAAATGcc                                                                                 <  4:24202/138‑1 (MQ=255)
                                                      aCGGAAACTCCTTCAAGAGTGCCAAGCACAAGCTGTCCGGCATGCCCTTCAACAGTAGATACCGGGAATTCAGGTATATCTTCATATTTCAGCTTGACCGGATTTTCGATTTCGTCCGCCAACATGCCAAGACCTGAGc                                                                      >  3:291753/1‑139 (MQ=255)
                                                      aCGGAAACTCCTTCAAGAGTGCCAAGCACAAGCTGTCCGGCATGCCCTTCAACAGTAGATACCGGGAATTCAGGTATATCTTCATATTTCAGCTTGACCGGATTTTCGATTTCGTCCGCCAAAATGCCAAGACCTgcgc                                                                      >  4:208934/1‑139 (MQ=255)
                                                      aCGGAAACTCCTTCAAGAGTGCCAAGCACAAGCTGTCCGGCATGCCCTTCAACAGTAGATACCGGGAATTCAGGTATATCTACATATTTCAGCTTGACCGGATTTTCGATTTCGTCCGCCAAAATGCCAAGACCTGAGc                                                                      >  5:193091/1‑139 (MQ=255)
                                                      aCGGAAACTCCTTCAAGAGTGCCAAGCACAAGCTGTCCGGCATGCCCTTCAACAGTAGATACCGGGAATTCAGGTATATCTACATATTTCAGCTTGACCGGATTTTCGATTTCGTCCGCCAAAATGCCAAGACCTGAGc                                                                      >  5:189381/1‑139 (MQ=255)
                                                      aCGGAAACTCCTTCAAGAGTGCCAAGCACAAGCTGTCCGGCATGCCCTCCAACAGTAGATACCGGGAATTCAGGTATATCTTCATATTTCAGCTTGACCGGATTTTCGATTTCGTCCGCCAAAATGCCAAGACCTGAGc                                                                      >  1:267219/1‑139 (MQ=255)
                                                       cGGAAACTCCTTCAAGAGTGCCAAGCACAAGCTGTCCGGCATGCCCTTCAACAGTAGATACCGGGAATTCAGGTATATCTACATATTTCAGCTTGACCGGATTTTCGATTTCGTCCGCCAAAATGCCAAGACCTGAGcc                                                                     <  6:193091/139‑1 (MQ=255)
                                                       cGGAAACTCCTTCAAGAGTGCCAAGCACAAGCTGTCCGGCATGCCCTTCAACAGTAGATACCGGGAATTCAGGTATATCTACATATTTCAGCTTGACCGGATTTTCGATTTCGTCCGCCAAAATGCCAAGACCTGAGcc                                                                     <  6:189381/139‑1 (MQ=255)
                                                         gAAACTCCTTCAAGAGTGCCAAGCACAAGCTGTCCGGCATGCCCTTCAACAGTAGATACCGGGAATTCAGGTATATCTTCATATTTCAGCTTGACCGGATTTTCGATTTCGTCCGCCAAAATGCCAAGACCt                                                                          >  6:39100/1‑132 (MQ=255)
                                                         gAAACTCCTTCAAGAGTGCCAAGCACAAGCTGTCCGGCATGCCCTTCAACAGTAGATACCGGGAATTCAGGTATATCTTCATATTTCAGCTTGACCGGATTTTCGATTTCGTCCGCCAAAATGCCAAGACCt                                                                          <  5:39100/132‑1 (MQ=255)
                                                            aCTCCTTCAAGAGTGCCAAGCACAAGCTGTCCGGCATGCCCTTCAACAGTAGATACCGGGAATTCAGGTATATCTTCATATTTCAGCTTGACCGGATTTTCGATTTCGTCCGCCAAAATGCCAAGACCTGAGCCTaaaa                                                                <  1:7146/139‑1 (MQ=255)
                                                             cTCCTTCAAGAGTGCCAAGCACAAGCTGTCCGGCATGCCCTTCAACAGTAGATACCGGGAATTCAGGTATATCTTCATATTTCAGCTTGACCGGATTTTCGATTTCGTCCGCCAAAATGCCAAGACCTGAGCCTAAAAt                                                               <  3:164014/139‑1 (MQ=255)
                                                                cTTCAAGAGTGCCAAGCACAAGCTGTCCGGCATGCCCTTCAACAGTAGATACCGGGAATTCAGGTATATCTTCATATTTCAGCTTGACCGGATTTTCGATTTCGTCCGCCAAAATGCCAAGACCTGAGCCTAAAATAAg                                                            >  7:4859/1‑139 (MQ=255)
                                                                           ccAAGCACAAGCTGTCCGGCATGCCCTTCAACAGTAGATACCGGGAATTCAGGTATATCTTCATATTTCAGCTTGACCGGATTTTCGATTTCGTCCGCCAAAATGCCAAGACCTGAGCCTAAAATAAGGCCg                                                        >  6:40475/1‑132 (MQ=255)
                                                                           ccAAGCACAAGCTGTCCGGCATGCCCTTCAACAGTAGATACCGGGAATTCAGGTATATCTTCATATTTCAGCTTGACCGGATTTTCGATTTCGTCCGCCAAAATGCCAAGACCTGAGCCTAAAATAAGGCCg                                                        <  5:40475/132‑1 (MQ=255)
                                                                           ccAAGCACAAGCTGTCCGGCATGCCCTTCAACAGTAGATACCGGGAATTCAGGTATATCTTCATATTTCAGCTTGACCGGATTTTCGATTTCGTCCGCCAAAATGCCAAGACCTGAGCCTAAAATAAGGCCGATCTTTg                                                 >  5:228264/1‑139 (MQ=255)
                                                                                     gCTGTCCGGCATGCCCTTCAACAGTAGATACCGGGAATTCAGGTATATCTTCATATTTCAGCTTGACCGGATTTTCGATTTCGTCCGCCAAAATGcc                                                                                 <  4:182751/97‑1 (MQ=255)
                                                                                     gCTGTCCGGCATGCCCTTCAACAGTAGATACCGGGAATTCAGGTATATCTTCATATTTCAGCTTGACCGGATTTTCGATTTCGTCCGCCAAAATGcc                                                                                 >  3:182751/1‑97 (MQ=255)
                                                                                         tCCGGCATGCCCTTCAACAGTAGATACCGGGAATTCAGGTATATCTTCATATTTCAGCTTGACCGGATTTTCGATTTCGTCCGCCAAAATGCCAAGACCTGAGCCTAAAATAAGGCCGCTCTTTGGAGATTCCGGCAgg                                   >  6:132464/1‑139 (MQ=255)
                                                                                          ccGGCATGCCCTTCAACAGTAGATACCGGGAATTCAGGTATATCTTCATATTTCAGCTTGACCGGATTTTCGATTTCGTCCGCCAAAATGCCAAGACCTGAGCCTAAAATAAGGCCGATCTTTGGAGATTCCGGCAGGt                                  >  1:23622/1‑139 (MQ=255)
                                                                                               aTGCCCTTCAACAGTAGATACCGGGAATTCAGGTATATCTTCATATTTCAGCTTGACCGGATTTTCGATTTCGTCCGCCAAAATGCCAAGACCTGAGCCTAAAATAAGGCCGATCTTTGGAGATTCCGGCAGGTTTTGt                             <  3:161316/139‑1 (MQ=255)
                                                                                                   ccTTCAACAGTAGATACCGGGAATTCAGGTATTTCTTCATATTTCAGCTTGACCGGATTTTCGATTTCGTCCGCCAAAATGCCAAGACCTGCGCCTAAAATAAGGCCGATCTTTGGAGATTCCGGCAGGTTTTGTTtaa                         >  5:166190/1‑139 (MQ=255)
                                                                                                             tAGATACCGGGAATTCAGGTATATCTTCATATTTCAGCTTGACCGGATTTTCGATTTCGTCCGCCAAAATGCCAAGACCTGAGCCTAAAATAAGGCCGATCTTTGGAGATTCCGGCAGGTTTTGTTTAATAAAAGCGGc               >  8:265737/1‑139 (MQ=255)
                                                                                                             tAGATACCGGGAATTCAGGTATATCTTCATATTTCAGCTTGACCGGATTTTCGATTTCGTCCGCCAAAATGCCAAGACCTGAGCCTAAAATAAGGACGATCTTTGGAGATTCCGGCAGGTTTTGTTTAATAAAAGCGGc               >  8:153277/1‑139 (MQ=255)
                                                                                                               gATACCGGGAATTCAGGTATATCTTCATATTTCAGCTTGACCGGATTTTCGATTTCGTCCGCCAAAATGCCAAGACCTGAGCCTAAAATAAGGCCGATCTTTGGAGATTCCGGCAGGTTTTGTTTAATAAAAGCGGCt              <  8:232957/138‑1 (MQ=255)
                                                                                                               gATACCGGGAATTCAGGTATATCTTCATATTTCAGCTTGACCGGATTTTCGATTTCGTCCGCCAAAATGCCAAGACCTGAGCCTAAAATAAGGCCGATCTTTGGAGATTCCGGCAGGTTTTGTTTAATAAAAGCGGCTg             >  7:74451/1‑139 (MQ=255)
                                                                                                               gATACCGGGAATTCAGGTATATCTTCATATTTCAGCTTGACCGGATTTTCGATTTCGTCCGCCAAAATGCCAAGACCTGAGCCTAAAATAAGGCCGATCTTTGGAGATTCCGGCAGGTTTTGTTTAATAAAAGCGGCTg             >  8:247245/1‑139 (MQ=255)
                                                                                                                aTACCGGTAATTCAGGTATATCTTCATATTTCAGCTTGACCGGATTTTCGATTTCGTCCGCCAAAATCCCAAGACCTGAGCCTAAAATAAGGCCGATCTTTGGATATTCCGGCAGGTTTTGTTTAATAAAAGCGGCTgc            <  5:15814/139‑1 (MQ=255)
                                                                                                                aTACCGGGAATTCAGGTATATCTTCATATTTCAGCTTGACCGGATTTTCGATTTCGTCCGCCAAAATGCCAAGACCTGAGCCTAAAATAAGGCCGATCTTTGGAGATTCCGGCAGGTTTTGTTTAATAAAAGCGGCTgc            <  3:119661/139‑1 (MQ=255)
                                                                                                                  aCCGGGAATTCAGGTATATCTTCATATTTCAGCTTGACCGGATTTTCGATTTCGTCCGCCAAAATGCCAAGACCTGAGCCTAAAATAAGGCCGATCTTTGGAGATTCCGGCAGGTTTTGTTTAATAAAAGCGGCTgcgc          >  6:164978/1‑139 (MQ=255)
                                                                                                                  aCCGGGAATTCAGGTATATCTTCATATTTCAGCTTGACCGGATTTTCGATTTCGTCCGCCAAAATGCCAAGACCTGAGCCTAAAATAAGGCCGATCTTTGGAGATTCCGGCAGGTTTTGTTTAATAAAAGCGGCTgcgc          >  5:33835/1‑139 (MQ=255)
                                                                                                                   ccGGGAATTCAGGTATATCTTCATATTTCAGCTTGACCGGATTTTCGATTTCGTCCGCCAAAATGCCAAGACCTGAGCCTAAAATAAGGCCGATCTTTGGAGATTCCGGCAGGTTTTGTTTAATAAAAGCGGCTGcgcg         >  5:262786/1‑139 (MQ=255)
                                                                                                                    cGGGAATTCAGGTATATCTTCATATTTCAGCTTGACCGGATTTTCGATTTCGTCCGCCAAAATGCCAAGACCTGAGCCTAAAATAAGGCCGATCTTTGGAGATTCCGGc                                      >  7:3202/1‑109 (MQ=255)
                                                                                                                    cGGGAATTCAGGTATATCTTCATATTTCAGCTTGACCGGATTTTCGATTTCGTCCGCCAAAATGCCAAGACCTGAGCCTAAAATAAGGCCGATCTTTGGAGATTCCGGc                                      <  8:3202/109‑1 (MQ=255)
                                                                                                                      ggAATTCAGGTATATCTTCATATTTCAGCTTGACCGGATTTTCGATTTCGTCCGCCAAAATGCCAAGACCTGAGCCTAAAATAAGGCCGATCTTTGGAGATTCGGGCAGGTTTTGTTTAATAAAAGCGGCTgc            >  4:84244/1‑133 (MQ=255)
                                                                                                                      ggAATTCAGGTATATCTTCATATTTCAGCTTGACCGGATTTTCGATTTCGTCCGCCAAAATGCCAAGACCTGAGCCTAAAATAAGGCCGATCTTTGGAGATTCCGGCAGGTTTTGTTTAATAAAAGCGGCTgc            <  3:84244/133‑1 (MQ=255)
                                                                                                                       gAATTCAGGTATATCTTCATATTTCAGCTTGACCGGATTTTCTATTTCGTCCGCCAAAATGCCAAGACCTGAGCCTAAAATAAGTCCGATCTTTGGAGATTCCGTCAGGTTTTGTTTAATAAAAGCTGCTGCGCGTTc      >  6:261648/1‑138 (MQ=255)
                                                                                                                       gAATTCAGGTATATCATCATATTTCAGCTTGACCGGATTTTCGATTTCGTCCGCCAAAATGCCAAGACCTGAGCCTAAAATAAGGCCGATCTTTGGAGCTTCCGGCTGCTTGAGTTTAATAAAAGCGGCTGCGCGTTc      >  6:224306/1‑138 (MQ=255)
                                                                                                                          ttCAGGTATATCTTCATATTTCAGCTTGACCGGATTTTCGATTTCGTCCGCCAAAATGCCAAGACCTGAGCCTAAAATAAGGCCGATCTTTGGAGATTCCGGCAGGTTTTGTTTAATAAAAGCGGCTGCGCGTTCAAtt  >  7:21081/1‑139 (MQ=255)
                                                                                                                                       |                                                                                                                             
ACTTTCTCCATTGAGTAGCCTTCATAAAAATGAAAGCGGCCCTGCATTGCAATGACGGAAACTCCTTCAAGAGTGCCAAGCACAAGCTGTCCGGCATGCCCTTCAACAGTAGATACCGGGAATTCAGGTATATCTTCATATTTCAGCTTGACCGGATTTTCGATTTCGTCCGCCAAAATGCCAAGACCTGAGCCTAAAATAAGGCCGATCTTTGGAGATTCCGGCAGGTTTTGTTTAATAAAAGCGGCTGCGCGTTCAATT  >  CP006881/2264830‑2265090

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑