Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 2,691,907 C→T 8.6% E40E (GAG→GAA)  U712_13870 ← Glutamate‑1‑semialdehyde 2,1‑aminomutase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,691,9070CT 8.6% 132.5 / 2.4 47E40E (GAG→GAA) U712_13870Glutamate‑1‑semialdehyde 2,1‑aminomutase
Reads supporting (aligned to +/- strand):  ref base C (24/17);  new base T (2/2);  total (26/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.06e-01

GTCCCGTATTCAGCCACTTTTTTGAGGCTTTCTACGACGCGGTCATTTGTATGCCCTAAAATTAAAGGCCCCCATGACAAGACGTAGTCAATATATTCATTCCCGTCAATATCAAAGATTTTCGAGCCTTTTCCGCGCTCCATAAAAATCGGGTCCATGTCTACCGATTTAAATGCGCGAACGGGACTGTTCACACCGCCCGGCATGAGTTTTTGCGCTTCTTTAAAAGCCGTTTTTGATTTTTCATAGCTTCTCATCTGCCTCAACTCCTGT  >  CP006881/2691770‑2692042
                                                                                                                                         |                                                                                                                                       
gTCCCGTATTCAGCCACTTTTTTGAGGCTTTCTACGACGCGGTCATTTGTATGCCCTAAAATTAAAGGCCCCCATGACAAGACGTAGTCAATATATTCATTCCCGTCAATATCAAAGCTTTTCGAGCCTTTTCCGCGCt                                                                                                                                        >  8:129273/1‑139 (MQ=255)
gTCCCGTATTCAGCCACTTTTTTGAGGCTTTCTACGACGCGGTCATTTGTATGCCCTAAAATTAAAGGCCCCCATGACAAGACGTAGTCAATATATTCATTCCCGTCAATATCAAAGATTTTCGAGCCTTTTCCGCGCt                                                                                                                                        >  2:213881/1‑139 (MQ=255)
gTCCCGTATTCAGCCACTTTTTTGAGGCTTTCTACGACGCGGTCATTTGTATGCCCTAAAATTAAAGGCCCCCATGACAAGACGTAGTCAATATATTCATTCCCGTCAATATCAAAGATTTTCGAGCCTTTTCCGCGCt                                                                                                                                        >  5:296816/1‑139 (MQ=255)
gTCCCGTATTCAGCCACTTTTTTGAGGCTTTCTACGACGCGGTCATTTGTATGCCCTAAAATTAAAGGCCCCCATGACAAGACGTAGTCAATATATTCATTCCCGTCAATATCAAAGATTTTCGAGCCTTTTCCGCGCt                                                                                                                                        >  7:281136/1‑139 (MQ=255)
 tCCCGTATTCAGCCACTTTTTTGAGGCTTTCTACGACGCGGTCATTTGTATGCCCTAAAATTAAAGGCCCCCATGACAAGACGTAGTCAATATATTCATTCCCGTCAATATCAAAGATTTTCGAGCCTTTTCCGCGCTc                                                                                                                                       >  3:251685/1‑139 (MQ=255)
      tATTCAGCCACTTTTTTGAGGCTTTCTACGACGCGGTCATTTGTATGCCCTAAAATTAAAGGCCCCCATGACAAGACGTAGTCAATATATTCATTCCCGTCAATATCAAAGATTTTCGAGCCTTTTCCGCGCTCCATa                                                                                                                                   >  5:140779/1‑138 (MQ=255)
           aGCCACTTTTTTGAGGCTTTCTACGACGCGGTCATTTGTATGCCCTAAAATTAAAGGCCCCCATGACAAGACGTAGTCAATATATTCATTCCCGTCAATATCAAAGATTTTCGAGCCTTTTCCGCGCTCCATAAAAATc                                                                                                                             <  8:63793/139‑1 (MQ=255)
             ccACTTTTTTGAGGCTTTCTACGACGCGGTCATTTGTATGCCCTAAAATTAAAGGCCCCCATGACAAGACGTAGTCAATATATTCATTCCCGTCAATATCAAAGATTTTCGAGCCTTTTCCGCGCTCCATAAAAATCgg                                                                                                                           >  3:250567/1‑139 (MQ=255)
              cACTTTTTTGAGGCTGTCTACGACGCGGTCATTTGTATGCCCTAAAATTAAAGGCCCCCATGACAAGACGTAGTCAATATATTCATTCCCGTCAATATCAAAGATTTTCGAGCCTTTTCCGCGCTCCATAAAAATCggg                                                                                                                          <  7:104137/139‑1 (MQ=255)
                 tttttgGAGGCTTTCTACGACGCCGTCATTTGTATGCCCTAAAATTAAAGGCCCCCTTGACAAGACGTAGTCAATATATTCATTCCCGTCAATATCAAAGATTTTCGAGCCTTTTCCGCGCTCCATAAAAATCGGGTcc                                                                                                                       <  6:162942/139‑1 (MQ=255)
                         ggCTTTCTACGACGCGGTCATTTGTATGCCCTAAAATTAAAGGCCCCCATGACAAGACGTAGTCAATATATTCATTGCCGTCAATATCAAAGATTTTCGAGGCTTTTCCGCGCTCCATAAAAATCGGGTCCATGTCTAc                                                                                                               <  2:3249/139‑1 (MQ=255)
                         ggCTTTCTACGACGCGGTCATTTGTATGCCCTAAAATTAAAGGCCCCCATGACAAGACGTAGTCAATATATTCATTCCCGTCAATATCAAAGATTTTCGAGCCTTTTCCGCGCTCCATAAAAATCGGGTCCATGTCTAc                                                                                                               <  1:213881/139‑1 (MQ=255)
                                       cgGTCATTTGTATGCCCTAAAATTAAAGGCCCCCATGACAAGACGTAGTCAATATATTCCTTCCCGTCAATATCAAAGATTTTCGAGCCTTTTCCGCGCTCCATAAAAATCGGGTCCATGTCTACCGATTTAAATgcgc                                                                                                 >  4:144395/1‑139 (MQ=255)
                                       cgGTAATTTTTATGCCCTAAAATTAAAGGCCCCCATTAAAATACGTAGTAAATATATTCATTCCCGTAATTATCAAAGATTTTCGAGCCTTTTCCGCGATCCATAAAAATCGTGGCAATGTATACCTATTTAAATgcgc                                                                                                 <  4:250567/139‑1 (MQ=255)
                                        ggTCATTTGTATGCCCTAAAATTAAAGGCCCCCATGACAAGACGTAGTCAATATATTCATTCCCGTCAATATCAAAGATTTTCGAGCCTTTTCCGCGCTCCATAAAAATCGGGTCCATGTCTTCCGATTTAAATgcgc                                                                                                 >  5:33530/1‑138 (MQ=255)
                                          taaTTTGTATGCCCAAAAATTAAAGGCCCCCATGAAAAGACTTAGTAAAAATATACATTCCCGTCAATATAAAAGATTTTCGAGCCTTTTCCGCGCTCAATAAAAATCGGGTCCATGTCTACCGATTTAAATGCGCGaa                                                                                              <  6:33530/137‑1 (MQ=255)
                                          tCATTTGTATGCACTAAAATTAACGGCCCCCATGACAAGACGTAGTCAATATATTCATTCCCGTCAATATAAAAGATTTTCGAGCCTTTTCCGCGCTCCATAAAAATCGGGTCCATGTCTACCGATTTAAATGCGCGaa                                                                                              <  6:218807/139‑1 (MQ=255)
                                               tGTATGCCCTAAAATTAAAGGCCCCCATGACAAGACGTAGTCAATATATTCATTCCCGTCAATATCAAAGATTTTCGAGCCTTTTCCGCGCTCCATAAAAATCGGGTCCATGTCTACCGATTTAAATGCGCGAACggg                                                                                          <  3:293568/138‑1 (MQ=255)
                                               tGTATGCCCTAAAATTAAAGGCCCCCATGACAAGACGTAGTCAATATATTCATTCCCGTCAATATCAAAGATTTTCGAGCCTTTTCCGCGCTCCATAAAAATCGGGTCCATGTCTACCGATTTAAATGCGCGAACggg                                                                                          <  3:289239/138‑1 (MQ=255)
                                               acacagCCCTAAAATTAACGGCCCCCATGACATCTCGTAGTCAATATATTCATTCCCGTCAATATCAAAGATTTTCGTGCCTTTTCCGCGCTCCATAAAAATCGGGTCCATGTCTACCGATTTAAATGCGCGAACGGGa                                                                                         <  6:72881/134‑1 (MQ=255)
                                                gTATTCCCAAAAATTAAAGGCCCCAAAGACAAGACGTTTTAAATTTATTCATTCCCGTCAATATCAAAGATATTCGAGCCTTTTCCGCGCTCCAAAAAAATCGGGTCCATGTCTACCGATTTAAATGCGCGAACGGGa                                                                                         <  8:281136/138‑1 (MQ=255)
                                                gTATGCCCTAAAATTAACGGACCCCCTGACAAGACTTAGTATATATATTCATACCCCTCAATATCAAAGATTTTCGAGCCTTTGCCGCGCACCAAAAACATCGGGTCCATGTCTACCGATTTAAATGCGCTAACGGGa                                                                                         <  4:251685/138‑1 (MQ=255)
                                                gTATGCCCTAAAATTAAAGGCCCCCATGACAAGACGTAGTCAATATATTCATTCCCGTCAATATCAAAGATTTTCGAGCCTTTTCCGCGCTCCATAAAAATCGGGTCCATGTCTACCGATTTAAATGCGCGAACGGGa                                                                                         <  6:296816/138‑1 (MQ=255)
                                                      ccTAAAATTAAAGGCCCCCATGACAAGACGTAGTCAATATATTCATTCCCGTCAATATCAAAGATTTTCGAGCCTTTTCCGCGCTCCATAAAAATCGGGTCCATGTCTACCGATTTAAATGCGCGAACGGGACTGTTca                                                                                  <  5:143474/139‑1 (MQ=255)
                                                        tAAAATTAAAGGCCCCCATGCCAAGACGTAGTCAATAAATTCATACCCGTCAATATCAAAGATTATCGAGCCTTTTCCGCGCTCCATAAAAATCGGGTCCATGTCTACAGATTTAAATGCGCGAACGGGACTGTTcaca                                                                                >  3:213326/1‑139 (MQ=255)
                                                                aaGGCCCCCATGACAAGACGTAGTCAATATATTCATTCCCGTCAATATCAAAGATTTTCGAGCCTTTTCCGCGCTCCATAAAAATCGGGTCCATGTCTACCGATTTAAATGCGCGAACGGGACTGTTCACAccctgtct                                                                        >  5:72881/1‑133 (MQ=255)
                                                                                gACGTAGTCAATATATTCATTCCCGTCAATATCAAAGATTTTCGAGCCTTTTCCGCGCTCCATAAAAATCGGGTCCATGTCTACCGATTTAAATGCGCGAACGGGACTGTTCACACCGCCCGGCATGAGTTTTTGCGct                                                        >  2:1831/1‑139 (MQ=255)
                                                                                gACGTAGTCAATATATTCATTCCCGTCAATATCAAAGATTTTCGAGCCTTTTCCGCGCTCCATAAAAATCGGGTCCATGTCTACCGATTTAAATGCGCGAACGGGACTGTTCACACCGACCGGCATGAGTTTTTGCGct                                                        >  4:51949/1‑139 (MQ=255)
                                                                                     aGTCAATATATTCATTCCCGTCAATATCAAAGATTTTCGAGCCTTTTCCGCGCTCCATAAAAATCGGGTCCATGTCTACCGATTTAAATGCGCGAACGGGACTGTTCACACCGCCCGGCATGAGTTTTTGCGcttctt                                                    >  7:167410/1‑138 (MQ=255)
                                                                                           tatatTCATTCCCGTCAATATCAAAGATTTTCGAGCCTTTTCCGCGTTCCATAAAAATCGGGTCCATGTCTACCGATTTAAATGCGCGAACGGGa                                                                                         >  8:240842/1‑95 (MQ=255)
                                                                                           tatatTCATTCCCGTCAATATCAAAGATTTTCGAGCCTTTTCCGCGTTCCATAAAAATCGGGTCCATGTCTACCGATTTAAATGCGCGAACGGGa                                                                                         >  8:236708/1‑95 (MQ=255)
                                                                                           tatatTCATTCCCGTCAATATCAAAGATTTTCGAGCCTTTTCCGCGTTCCATAAAAATCGGGTCCATGTCTACCGATTTAAATGCGCGAACGGGa                                                                                         <  7:236708/95‑1 (MQ=255)
                                                                                           tatatTCATTCCCGTCAATATCAAAGATTTTCGAGCCTTTTCCGCGTTCCATAAAAATCGGGTCCATGTCTACCGATTTAAATGCGCGAACGGGa                                                                                         <  7:240842/95‑1 (MQ=255)
                                                                                           tatatTCATTCCCGTCAATATCAAAGATTTTCGAGCCTTTTCCGCGCTCCATAAAAATCGGGTCCATGTCTACCGATTTAAATGCGCGAACGGGACTGTTCACACCGCCCGGCATGAGTTTTTGCGCTTCTTTAAAAGc                                             >  5:77834/1‑139 (MQ=255)
                                                                                                    tcCCGTCAATATCAAAGATTTTCGAGCCTTTTCCGCGCTCCATAAAAATCGGGTCCATGTCTACCGATTTAAATGCGCGAACGGGACTGTTCACACCGCCCGGCATGAGTTTTTGCGCTTCTTTAAAAGCCGTTTTTGa                                    >  5:190648/1‑139 (MQ=255)
                                                                                                     cccGTCAATATCAAAGATTTTCGAGCCTTTTCCGCGCTCCATAAAAATCGGGTCCATGTCTACCGATTTAAATGCGCGAACGGGACTGTTCACACCGCCCGGCATGAGTTTTTGCGCTTCTTTAAAAGCCGTTTTTGAt                                   >  5:108356/1‑139 (MQ=255)
                                                                                                     cccGTCAATATCAAAGATTTTCGAGCCTTTTCCGCGCTCCATAAAAATCGGGTCCATGTCTACCGATTTAAATGCGCGAACGGGACTGTTCACACCGCCCGGCATGAGTTTTTGCGCTTCTTTAAAAGCCGTTTTTGAt                                   >  7:266891/1‑139 (MQ=255)
                                                                                                     cccGTCAATATCAAAGATTTTCGAGCCTTTTCCGCGCTCCATAAAAATCGGGTCCATGTCTACCGATTTAAATGCGCGAACGGGACTGTTCACACCGCCCGGCATGAGTTTTTGCGCTTCTTTAAAAGCCGTTTTTGAt                                   >  3:291751/1‑139 (MQ=255)
                                                                                                        gTCAATATCAAAGATTTTCGAGCCTTTTCCGCGCTCCATAAAAATCGGGTCCATGTCTACCGATTTAAAAGCGCTACCGGGACTGTACACACCGCCCGGCATGAGTTTTTGCGCTTCTTTAAATGCCGGTTTTGAtttt                                >  1:243945/1‑139 (MQ=255)
                                                                                                                cAAAGATTTTCGAGCCTTTTCCGCGCTCCATAAAAATCGGGTCCATGTCTACCGATTTAAATGCGCGAACGGGACTGTTCACACCGCCCGGCATGAGTTTTTGCGCTTCTTTAAAAGCCGTTTTTGATTTTTCATAGCt                        >  2:293109/1‑139 (MQ=255)
                                                                                                                 aaaGATTTTCGAGCCTTTTCCGCGCTCCATAAAAATCGGGTCCATGTCTACCGATTTAAATGCGCGAACGGGACTGTTCACACCGCCCGGCATGAGTTTTTGCGCTTCTTTAAAAGCCGTTTTTGATTTTTCATAGCtt                       <  6:77834/139‑1 (MQ=255)
                                                                                                                      ttttCGAGCCTTTTCCGCGCTCCATAAAAATCGGGTCCATGTCTACCGATTTAAATGCGCGAACGGGACTGTTCACACCGCCCGGCATGAGTTTTTGCGCTTCTTTAAAAGCCGTTTTTGATTTTTCATAGCTTCTCAt                  <  4:70309/139‑1 (MQ=255)
                                                                                                                       tttCGAGCCTTTTCCGCGCTCCATAAAAATCGGGTCCATGTCTACCGATTTAAATGCGCGAACGGGACTGTTCACACCGCCCGGCATGAGTTTTTGCGCTTCTTTAAAAGCCGTTTTTGATTTTTCATAGCTTCTCATc                 >  5:17986/1‑139 (MQ=255)
                                                                                                                       tttCGAGCCTTTTCCGCGCTCCATAAAAATCGGGTCCATGTCTACCGATTTAAATGCGCGAACGGGACTGTTCACACCGCCCGGCATGAGTTTTTGCGCTTCTTTAAAAGCCGTTTTTGATTTTTCATAGCTTCTCATc                 >  4:103942/1‑139 (MQ=255)
                                                                                                                        ttCGAGCCTTTTCCGCGCTCCATAAAAATCGGGTCCATGTCTACCGATTTAAATGCGCGAACGGGACTGTTCACACCGCCCGGCATGAGTTTTTCCGCTTCTTAAAAAGCCGTTTTTGATTTTTCATAGCTTCTCATcg                >  5:48203/1‑138 (MQ=255)
                                                                                                                        ttCGAGCCTTTTACGCGCTCCATAAACATCGGGTCCATGTCTACCGATTTAAATGCGCGAACGGGACTGTTCACACCGCCCGGCATGAGTTTTTGCGCTTCTTTAAAAGCCGTTTTTGATTTTTCATAGCTTCTCATc                 <  6:140779/138‑1 (MQ=255)
                                                                                                                               cTTTTCCGCGATCCATAACAATCGGGTCCCTGTCTACCGATTTAAATGCGCGATCGGGACTGTTCACACCGCCCGGCATGAGTTTTTGCGCTGCTTTAAAAGCCGTTTTTGATTTTTCATAGCTTCTCATCTGCCTcca         <  3:144395/139‑3 (MQ=255)
                                                                                                                                       cgcTCCATAAAAATCGGGTCCATGTCTACCGATTTAAATGCGCGAACGGGACTGTTCACACCGCCCGGCATGAGTTTTTGCGCTTCTTTAAAAGCCGTTTTTGATTTTTCATAGCTTCTCATCTGCCTCAACTCCTGt  >  3:55910/1‑138 (MQ=255)
                                                                                                                                         |                                                                                                                                       
GTCCCGTATTCAGCCACTTTTTTGAGGCTTTCTACGACGCGGTCATTTGTATGCCCTAAAATTAAAGGCCCCCATGACAAGACGTAGTCAATATATTCATTCCCGTCAATATCAAAGATTTTCGAGCCTTTTCCGCGCTCCATAAAAATCGGGTCCATGTCTACCGATTTAAATGCGCGAACGGGACTGTTCACACCGCCCGGCATGAGTTTTTGCGCTTCTTTAAAAGCCGTTTTTGATTTTTCATAGCTTCTCATCTGCCTCAACTCCTGT  >  CP006881/2691770‑2692042

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑