Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP006881	2,705,346	T→C	6.1%	K33R (AAA→AGA)	U712_13925 ←	Trigger factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,705,346	0	T	C	6.1%	200.6 / 3.7	66	K33R (AAA→AGA)	U712_13925	Trigger factor
Reads supporting (aligned to +/- strand):  ref base T (32/30);  new base C (2/2);  total (34/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01

CTTTAGGGTATTCTACAGGAAGAAGAATATCCAAAGCATCTTGGTAAAGAGCTTCTACGCCAAAGCGCTGTTCGAATAATCCGCGAGGAATTTTACCTTTACGGAATCCAGGAATTGAAACTTGTTTTACTACTTTTTTAAATGCATCATCAAGTGCTGTTTTAAACGTTTCAGCATCAACTTCAACCGTTAAAACGCCTTCGTTGCCTTCTTGTTTTTCCCATTTTACAGACATGTGTTTCCCTCCAAAAATCTATTCAGG  >  CP006881/2705209‑2705470                                                                                                                                          |                                                                                                                             cTTTAGGGTATTCTACAGGAAGAAGAATATCCAAAGCATCTTGGTAAAGAGCTTCTACGCCAAAGCGCTGTTCGAATAATCCGCGAGGAATTTTACCTTTACGGAATCCAGGAATTGAAACTTGTTTTACTACttttt                                                                                                                              >  4:89657/1‑138 (MQ=255)  tttAGGGTATTCTACAGGAAGAAGAATATCCTAAGCATCTTGGTAAAGAGCTTCTACGCCAAAGCGCTGTTCGAATAATACGCGAGGAATTTTACCTTTACGGAATCCAGGAATTGAAACTTGTTTTACTACtttttt                                                                                                                             >  7:132798/1‑138 (MQ=255)      gggTATTCTACAGGAAGAAGAATATCCAAAGCATCTTGGTAAAGAGCTTCTACGCCAAAGCGCTGTTCGAATAATCCGCGAGGAATTTTACCTTTACGGAATCCAGGAATTGAAACTTGTTTTACTACTTTTTTAAAt                                                                                                                         <  5:123877/138‑1 (MQ=255)        gTATTCTACAGGAAGAAGAATATCCAAAGCATCTTGGTAAAGAGCTTCTACGCCAAAGCGCTGTTCGAATAATCCGCGAGGAATTTTACCTTTACGGAATCCAGGAATTGAAACTTGTTTTACTACTTTTTTAAATGca                                                                                                                      <  4:102451/139‑1 (MQ=255)           ttCTACAGGAAGAATAATATCCAATGCATCTTGGTAAAGAGCTTCTACGCCAAAGCGCTGTTCGATTAATCCGCGAGGAATTTTACATTTACGGAATCCAGCAATTGAAACTTGTTTTCCTACATTTTTAAATGcatca                                                                                                                   >  1:6842/1‑139 (MQ=255)           ttCTACAGGAAGAAGAATATCCAAAGCATCTTGGTAAAGAGCTTCTACGCCAAAGCGCTGTTCGAATAATCCGCGAGGAATTTTACCTTTACGGAATCCAGGAATTGAAACTTGTTTTACTACTTTTTTAAATGcatca                                                                                                                   >  4:105492/1‑139 (MQ=255)           ttCTACAGGAAGAAGAATATCCAAAGCATCTTGGTAAAGAGCTTCTACGCCAAAGCGCTGTTCGAATAATCCGCGAGGAATTTTACCTTTACGGAATCCAGGAATTGAAACTTGTTTTACTACTTTTTTAAATGcatca                                                                                                                   >  6:220850/1‑139 (MQ=255)           ttCTACAGGAAGAAGAATATCCAAAGCATCTTGGTAAAGAGCTTCTACGCCAAAGCGCTGTTCGAATAATCCGCGAGGAATTTTACCTTTACGGAATCCAGGAATTGAAACTTGTTTTACTACTTTTTTAAATGcatc                                                                                                                    >  2:278770/1‑138 (MQ=255)           ttCTACAGGAAGAAGAATATCCAAAGCATCTTGGTAAAGAGCTTCTACGCCAAAGCGCTGTTCGAATAATCCGCGAGGAATTTTACCTTTACGGAATCCAGGAATTGAAACTTGTTTTACTACTTTTTTAAATGca                                                                                                                      <  6:216023/136‑1 (MQ=255)           ttCTACAGGAAGAAGAATATCCAAAGCATCTTGGTAAAGAGCTTCTACGCCAAAGCGCTGTTCGAATAATCCGCGAGGAATTTTACCTTTACGGAATCCAGGAATTGAAACTTGTTTTACTACTTTTTTAAATGca                                                                                                                      >  5:216023/1‑136 (MQ=255)           ttCTACAGGAAGAAGAATATCCAAAGCATCTTGGTAAAGAGCTTCTACGCCAAAGCGCTGTTCGAATAATCCGAGAGGAATTTTACCTTTACGGAATCCAGGAATTGAAACTTGTTTTACTACTTTTTTAAATGcatca                                                                                                                   >  2:129729/1‑139 (MQ=255)           ttCTACAGGAAGAAGAATATCCAAAGCATCTTGGTAAAGAGCTTCTACGCCAAAGCCCTGTTCGAATAATCCGCGAGGAATATTACCTTTACGAAATCCAGGAATTGAAACTTGTTTTACTACTTTTTTAAATGcatc                                                                                                                    >  2:109837/1‑138 (MQ=255)            tCTACAGGAAGAAGAATATCCAAAGCATCTTGGTAAAGAGCTTCTACGCCAAAGCGCTGTTCGAATAATCCGCGAGGAATTTTACCTTTACGGAATCCAGGAATTGAAACTTGTTTTACTACTTTTTTAAATGcatcat                                                                                                                  >  4:198044/1‑139 (MQ=255)                  ggAAGAAGAATATCCAAAGCATCTTGGTAAAGAGCTTCTACGCCAAAGCGCTGTTCGAATAATCCGCGAGGAATTTTACCTTTACGGAATCCAGGAATTGAAACTTGTTTTACTACTTTTTTAAATGCATCATCAAGTg                                                                                                            <  3:284544/139‑1 (MQ=255)                   gaagaagaaTATCCAAAGCATCTTGGTAAAGAGCTTCTACGCCAAAGCGCTGTTCGAATAATCCGCGAGGAATTTTACCTTTACGGAATCCAGGAATTGAATCTTGTTTTACTACTTTTTTAAATGCATCATCAAGTGc                                                                                                           >  2:157805/1‑139 (MQ=255)                   gaagaagaaTATCCAAAGCATCTTGGTAAAGAGCTTCTACGCCAAAGCGCTGTTCGAATAATCCGCGAGGAATTTTACCTTTACGGAATCCAGGAATTGAAACTTGTTTTACTACTTTTTTAAATGca                                                                                                                      <  3:219036/128‑1 (MQ=255)                   gaagaagaaTATCCAAAGCATCTTGGTAAAGAGCTTCTACGCCAAAGCGCTGTTCGAATAATCCGCGAGGAATTTTACCTTTACGGAATCCAGGAATTGAAACTTGTTTTACTACTTTTTTAAATGca                                                                                                                      >  4:219036/1‑128 (MQ=255)                   gaagaagaaTATCCAAAGCATCTTGGTAAAGAGCTTCTACGCCAAAGCGCTGTTCGAATAATCCGCGAGGAATTTTACCTTTACGGAATCCAGGAATTGAAACTTGTTTTACTACTTTTTTAAATGCATCATCAAGTGc                                                                                                           <  7:136898/139‑1 (MQ=255)                   gaagaagaaTATCCAAAGCATCTTGGTAAAGAGCTTCTACGCCAAAGCGCGGTTCGAATAATCCGCGACGAATTTTACCTTTACGGAATCCAGGAATTGAAACTTGTGTTACTACTTTATTAAATGCATCATCAAGTGc                                                                                                           >  7:23671/1‑139 (MQ=255)                     agaagaaTATCCAAAGCATCTTGGTAAAGAGCTTCTACGCCAAAGCGCTGTTCGAATAATCCGCGAGGAATTTTACCTTTACGGAATCCAGGAATTGAAACTTGTTTTAGTACTTTTTTAAATGCATCATCAAGTGCTg                                                                                                         >  4:55398/1‑139 (MQ=255)                     agaagaaTATCCAAAGCATCTTGGTAAAGAGCTTCTACGCCAAAGCGCTGTTCGAATAATCCGCGAGGAATTTTACCTTTACGGAATCCAGGAATTGAAACTTGTTTTACTACTTTTTTAAATGCATCATCAAGTGCTg                                                                                                         >  2:246306/1‑139 (MQ=255)                        agaaTATCCAAAGCATCTTGGTAAAGAGCTTCTACGCCACAGCGCTGTTCGAATAATCCGCGAGGAATTTTACCTTTACGGAATCCAGGAATTGAAACTTGTTTTACTACTTTTTTAAATGCATCATCATGTGCTGgtt                                                                                                      >  2:203515/1‑139 (MQ=255)                          aaTATCCAAAGCATCTTGGTAAAGAGCTTCTACGCCAAAGCGCTGTTCGAATAATCCGCGAGGAATTTTACCTTTACGGAATCCAGGAATTGAAACTTGTTTTACTACTTTTTTAAATGCATCATCAAGTGCTGTTTTa                                                                                                    <  2:9534/139‑1 (MQ=255)                            tatCCAAAGCATCTTGGTAAAGAGCTTCTACGCCAAAGCGCTGTTCGAATAATCCGCGAGGAATTTTACCTTTACGGAATCCAGGAATTGAAACTTGTTTTACTACTTTTTTAAATGCATCATCAAGTGCTGTTTTaaa                                                                                                  <  1:117498/139‑1 (MQ=255)                              tCCAAAGCATCTTGGTAAAGAGCTTCTACGCCAAAGCGCTGTTCGAATAATCCGCGAGGAATTTTACCTTTACGGAATCCAGGAATTGAAACTTGTTTTACTACTTTTTTAAATGCATCATCAAGTGCTGTTTTAAACg                                                                                                >  2:19269/1‑139 (MQ=255)                                 aaaGCATCTTGGTAAAGAGCTTCTACGCCAAAGCGCTGTTCGAATAATCCGCGAGGAATTTTACCTTTACGGAATCCAGGAATTGAAACTTGTTTTACTACTTTTTTAAATGCATCATCAAGTg                                                                                                            >  3:153898/1‑124 (MQ=255)                                 aaaGCATCTTGGTAAAGAGCTTCTACGCCAAAGCGCTGTTCGAATAATCCGCGAGGAATTTTACCTTTACGGAATCCAGGAATTGAAACTTGTTTTACTACTTTTTTAAATGCATCATCAAGTg                                                                                                            <  4:153898/124‑1 (MQ=255)                                 aaaGCATCTTGGTAAAGAGCTTCTACGCCAAAGCGCTGTTCGAATAATCCGCGAGGAATTTTACCTTTACGGAATCCAGGAATTGAAACTTGTTTTACTACTTTTTTAAATGCATCATCAAGTGCTGTTTTAAACGttt                                                                                             <  2:231159/139‑1 (MQ=255)                                   aGCATCTTGGTAAATAGCTTCTACGCCAAAGCGCTGTTCGAATAATCCGCGAGGAATTTTAACTTTACGGAATCCAGGAATTGAAACTTGTTTTACTACTTTTTTAAATGCATCATCAAGTGCTGTTTTAAACGTTTCa                                                                                           <  5:13693/139‑1 (MQ=255)                                   aGCATCTTGGTAAAGAGCTTCTACGCCAAAGCGCTGTTCGAATAATCCGCGAGGAATTTTACCTTTACGGAATCCAGGAATTGAAACTTGTTTTACTACTTTTTTAAATGCATCATCAAGTGCTGTTTTAAACGttt                                                                                             >  7:129719/1‑137 (MQ=255)                                      aTCTTGGTAAAGAGCTTCTACGCCAAAGCGCTGTTCGAATAATCCGCGAGGAATTTTACCTTTACGGAATCCAGGAATTGAAACTTGTTTTACTACTTTTTTAAATGCATCATCAAGTGCTGTTTTAAACGTTTCAGCa                                                                                        <  8:129719/139‑1 (MQ=255)                                        cTTGGTAAAGAGCTTCTACGCCAAAGCGCTGTTCGAATAATCCGCGAGGAATTTTACCTTTACGGAATCCAGGAATTGAAACTTGTTTTACTACTTTTTTAAATGCATCATCAAGTGCTGTTTTAAACGTTTCAGCAt                                                                                       >  3:9634/1‑138 (MQ=255)                                           ggTAAAGAGCTTCTACGCCAAAGCGCTGTTCGAATAATCCGCGAGGAATTTTACCTTTACGGAATCCAGGAATTGAAACTTGTTTTACTACTTTTTTAAATGCATCATCAAGTGCTGTTTTAAACGTTTCAGCATc                                                                                      <  8:161443/136‑1 (MQ=255)                                             tAAAGAGCTTCTACGCCAAAGCGCTGTTCGAATAATCCGCGAGGAATTTTACCTTTACGGAATCCAGGAATTGAAACTTGTTTTACTACTTTTTTAAATGCATCATCAAGTGCTGTTTTAAACGTTTCAGCa                                                                                        <  8:178356/132‑1 (MQ=255)                                             tAAAGAGCTTCTACGCCAAAGCGCTGTTCGAATAATCCGCGAGGAATTTTACCTTTACGGAATCCAGGAATTGAAACTTGTTTTACTACTTTTTTAAATGCATCATCAAGTGCTGTTTTAAACGTTTCAGCa                                                                                        >  7:178356/1‑132 (MQ=255)                                               aaGAGCTTCTACGCCAAAGCGCTGTTCGAATAATCCGCGAGGAATTTTACCTTTACGGAATCCAGGAATTGAAACTTGTTTTACTACTTTTTTAAATGCATCATCAAGTGCTGTTTTAAACGTTTCAGCATCAACTTc                                                                                <  3:89657/138‑1 (MQ=255)                                                 gagCTTCTACGCCAAAGCGCTGTTCGAATAATCCGCGAGGAATTTTACCTTTACGGAATCCAGGAATTGAAACTTGTTTTACTACTTTTCTAAATGCATCATCAAGTGCTGTTTTAAACGTTTCAGCATCAACTTCAAc                                                                             >  5:188784/1‑139 (MQ=255)                                                 gagCTTCTACGCCAAAGCGCTGTTCGAATAATCCGCGAGGAATTTTACCTTTACGGAATCCAGGAATTGAAACTTGTTTTACTACTTTTCTAAATGCATCATCAAGTGCTGTTTTAAACGTTTCAGCATCAACTTCAAc                                                                             >  5:188787/1‑139 (MQ=255)                                                      tCTACGCCAAAGCGCTGTTCGAATAATCCGCGAGGAATTTTACCTTTACGGAATCCAGGAATTGAAACTTGTTTTACTACTTTTTTAAATGCATCATCAAGTGCTGTTTTAAACGTTTCAGCATCAACTTCAACCGtt                                                                         >  5:111228/1‑138 (MQ=255)                                                      tCTACGCCAAAGCGCTGTTCGAATAATCCGCGAGGAATTTTACCTTTACGGAATCCAGGAATTGAAACTTGTTTTACTACTTTTTTAAATGCATCATCAAGTGCTGTTTTAAACGTTTCAGCATCAACTTCAACCGtt                                                                         >  1:28185/1‑138 (MQ=255)                                                               aaGCGCTGTTCGAATAATCCGCGAGGAATTTTACCTTTACGGAATCCAGGAATTGAAACTTGTTTTACTACTTTTTTAAATGCATCATCAAGTGCTGTTTTAAACGTTTCAGCATCAACTTCAACCGTTAAAACGCCtt                                                               >  2:126450/1‑139 (MQ=255)                                                                    cTGTTCGAATAATCCGCGAGGAATTTTACCTTTACGGAATCCAGGAATTGAAACTTGTTTTACTACTTTTTTAAATGCATCATCAAGAGCTGTTTTAAACGTTTCAGAATCATCTTCAACCGTTAAAACGCCTTCGTTg                                                          >  6:150591/1‑139 (MQ=255)                                                                     tGTTCGAATAATCCGCGAGGAATTTTACCTTTACGGAATCCAGGAATTGAAACTTGTTTTACTACTTTTTTAAATGCATCATCAAGTGCTGTTTTAAACGTTTCAGCATCAACTTCAACCGTTAAAACGCCTTCGTTGc                                                         >  6:74954/1‑139 (MQ=255)                                                                       ttCGAATAATCCGCGAGGAATTTTACCTTTACGGAATCCAGGAATTGAAACTTGTTTTACTACTTTTTTAAATGca                                                                                                                      <  6:248786/76‑1 (MQ=255)                                                                       ttCGAATAATCCGCGAGGAATTTTACCTTTACGGAATCCAGGAATTGAAACTTGTTTTACTACTTTTTTAAATGca                                                                                                                      >  5:248786/1‑76 (MQ=255)                                                                                 ccGCGAGGAATTTTACCTTTACGGAATCCAGGAATTGAAACATGTTTTACTACTTTTTTAAATGCATCATCAAGTGCTGTTTTAAACGTTTCAGCATAAAATTCAACCGTTAAAACCCATTCGATGCCTTCTAGttttt                                             >  4:261314/1‑139 (MQ=255)                                                                                   gcgAGGAATTTTACCTTTACGGAATCCAGGAATTGAAACTTGTTTTACTACTTTTTTAAATGCATCATCAAGTGCTGTTTTAAACGTTTCAGCATCAACTTCAACCGTTAAAACGCCTTCGTTGCCTTCTTGTTTTTcc                                           <  5:174717/139‑1 (MQ=255)                                                                                   gcgAGGAATTTTACCTTTACGGAATCCAGGAATTGAAACTTGTTTTACTACTTTTTTAAATGCATCATCAAGTGCTGTTTTAAACGTTGCAGCATGCACTTCAACCGTTAAAACGCCTTCGTTGCCTTCTTGTTTTTcc                                           <  1:109837/139‑1 (MQ=255)                                                                                     gAGGAATTTTACCTTTACGGAATCCAGGAATTGAAACTTGTTTTACTACTTTTTTAAATGCATCATCAAGTGCTGTTTTAAACGTTTCAGCATCAACTTCAACCGTTAAAACGCCTTCGTTGCCTTCTTGTTTTTCCCa                                         <  6:167983/139‑1 (MQ=255)                                                                                     gAGGAATTTTACCTTTACGGAATCCAGGAATTGAAACTTGTTTTACTACTTTTTTAAATGCATCATCAAGTGCTGTTTTAAACGTTTCAGCATCAACTTCAACCGTTAAAACGCCTTCGTTGCCTTCTTGTTTTTCCCa                                         <  1:129729/139‑1 (MQ=255)                                                                                     gAGGAATTTTACCTTTACGGAATCCAGGAATTGAAACTTGTTTTACTACTTTTTTAAATGCATCATCAAGTGCTGTTTTAAACGTTTCAGCATCAACTTCAACCGTTAAAACGCCTTCGTTGCCTTCTTGTTTTTCCCa                                         <  1:278770/139‑1 (MQ=255)                                                                                          aTTTTACCTTTACGGAATCCAGGAATTGAAACTTGTTTTACTACTTTTCTAAATGCATCATCAAGTGCTGTTTTAAACGTTTCAGCATCAACTTCAACCGTTAAAACGCCTTCGTTGCCTTCTTGTTTTTCCCATTTTa                                    <  6:188784/139‑1 (MQ=255)                                                                                          aTTTTACCTTTACGGAATCCAGGAATTGAAACTTGTTTTACTACTTTTCTAAATGCATCATCAAGTGCTGTTTTAAACGTTTCAGCATCAACTTCAACCGTTAAAACGCCTTCGTTGCCTTCTTGTTTTTCCCATTTTa                                    <  6:188787/139‑1 (MQ=255)                                                                                             ttACCTTTACGGAATCCAGGAATTGAAACTTGTTTTACTACTTTTTTAAATGCATCATCAAGTGCTGTTTTAAACGTTTCAGCATCAACTTCAACCGTTAAAACGCCTTCGTTGCCTTCTTGTTTTTCCCATTTTACa                                  <  4:160286/138‑1 (MQ=255)                                                                                                   ttACGGAATCCAGGAATTGAAACTTGTTTTACTACTTTTTTAAATGCATCATCAAGTGCTGTTTTAAACGTTTCAGCATCAACTTCAACCGTTAAAACGCCTTCGTTGCCTTCTTGTTTTTCCCATTTTACAGACAtgt                           >  5:93447/1‑139 (MQ=255)                                                                                                         aaTCCAGGAATTGAAACTTGTTTTACTACTTTTTTAAATGCATCATCAAGTGCTGTTTTAAACGTTTCAGCATCAACTTCAACCGTTAAAACGCCTTCGTTGCCTTCTTGTTTTTCCCATTTTACAGACATGTGTTTcc                     <  1:246306/139‑1 (MQ=255)                                                                                                              aGGAATTGAAACTTGTTTTACTACTTTTTTAAATGCATCATCAAGTGCTGTTTTAAACGTTTCAGCATCAACTTCAACCGTTAAAACGCCTTCGTTGCCTTCTTGTTTTTCCCATTTTACAGACATGTGTTTCCCTCCa                <  5:289955/139‑1 (MQ=255)                                                                                                              aGGAATTGAAACTTGTTTTACTACTTTTTTAAATGCATCATCAAGTGCTGTTTTAAACGTTTCAGCATCAACTTCAACCGTTAAAACGCCTTCGTTGCCTTCTTGTTTTTCCCATTTTACAGACATGTGTTTCCCTCCa                <  5:74954/139‑1 (MQ=255)                                                                                                                   ttGAAATTTTTTTTACTACTTTTTTAAATGCATCATCAAGTGCTGTTTTAAACTTTTCAGCATCTACTTCAACCGTTAAACCGCCTTCGTTGCATTCTTGTTTTTCCCATTTTACAGACATGTGTTTCACTCCaaaaa            >  4:207621/1‑138 (MQ=255)                                                                                                                   ttGAAACTTGTTTTACTACTTTTTTAAATGCATCATCAAGTGCTGTTTTAAACGTTTCAGCATCAACTTCAACCGTTAAAACGCCTTCGTTGCCTTCTTGTTTTTCCCATTTTACAGACATGTGTTTCCCTCCAAAAAt           >  8:202348/1‑139 (MQ=255)                                                                                                                   ttGAAACTTGTTTTACTACTTTTTTAAATGCATCATCAAGTGCTGTTTTAAACGTTTCAGCATCAACTTCAACCGTTAAAACGCCTTCGTTGCCTTCTTGTTTTTCCCATTTTACAGACATGTGTTTCCCTCCAAAAAt           <  1:280523/139‑1 (MQ=255)                                                                                                                   ttGAAACTTGTTTTACTACTTTTTTAAATGCATCATCAAGTGCTGTTTCAAACGTTTCAGCATCAACTTCAACCGTTAAAACGCCTTCGTTGCCTTCTTGTTTTTCCCATTTTACAGACATGTGTTTCCCTCCAAAAAt           <  6:215781/139‑1 (MQ=255)                                                                                                                      aaaCTTGTTTTACTACTTTTTTAAATGCATCATCAAGTGCTGTTTTAAACGTTTCAGCAt                                                                                       <  7:113717/60‑1 (MQ=255)                                                                                                                      aaaCTTGTTTTACTACTTTTTTAAATGCATCATCAAGTGCTGTTTTAAACGTTTCAGCAt                                                                                       >  8:113717/1‑60 (MQ=255)                                                                                                                          ttGTTTTACTACTTTTTTAAATGCATCATCAAGTGCTGTTTTAAACGTTTCAGCATCAACTTCAACCGTTAAAACGCCTTCGTTGCCTTCTTGTTTTTCCCATTTTAAAGACATGTGTTTCCCTCCAAAAATCTAtt      >  2:171926/1‑137 (MQ=255)                                                                                                                           tGTTTTACTACTTTTTTAAATGCATCATCAAGTGCTGTTTTAAACGTTTCAGCATCAACTTCAACCGTTAAAACGCCTTCGTTGCCTTCTTGTTTTTCCCATTTTACAGACATGTGTTTCCCTCCAAAAATCTATTCAg   >  8:254460/1‑139 (MQ=255)                                                                                                                            gTTTTACTACTTTTTTAAATGCATCATCAAGTGCTGTTTTAAACGTTTCAGCATCAACTTCAACCGTTAAAACGCCTTCGTTGCCTTCTTGTTTTTCCCATTTTACAGACATGTGTTTCCCTCCAAAAATCTATTCa    <  3:105492/137‑1 (MQ=255)                                                                                                                             ttttACTACTTTTTTAAATGCATCATCAAGTGCTGTTTTAAACGTTTCAGCATCAACTTCAACCGTTAAAACGCCTTCGTTGCCTTCTTGTTTTTCCCATTTTACAGACATGTGTTTCCCTCCAAAAATCTATTCAgg  <  8:230082/138‑1 (MQ=255)                                                                                                                                          |                                                                                                                             CTTTAGGGTATTCTACAGGAAGAAGAATATCCAAAGCATCTTGGTAAAGAGCTTCTACGCCAAAGCGCTGTTCGAATAATCCGCGAGGAATTTTACCTTTACGGAATCCAGGAATTGAAACTTGTTTTACTACTTTTTTAAATGCATCATCAAGTGCTGTTTTAAACGTTTCAGCATCAACTTCAACCGTTAAAACGCCTTCGTTGCCTTCTTGTTTTTCCCATTTTACAGACATGTGTTTCCCTCCAAAAATCTATTCAGG  >  CP006881/2705209‑2705470

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑