Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP006881	2,922,084	G→A	6.1%	A272V (GCA→GTA)	U712_15050 ←	Leucyl‑tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,922,084	0	G	A	6.1%	215.1 / 2.5	66	A272V (GCA→GTA)	U712_15050	Leucyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base G (28/33);  new base A (2/2);  total (31/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.83e-01

TACAGGATTGATCGCATACGCTCCTGTAAATACGCCTGTCTTTGTTTTCGCAAGATCTGTGCGTTCTAGGTCACTCTTTGATTGAATTTCTTTGATATAAGCTTCAACAGCTTCTTTTTGCTCTGCCGTTGTGATGTTTTCCACCAATGCGTGTTCCGGGGCAAGGACAGTGTATGTAGCGCCAAACAGCGTATCTGGTCTTGTTGTAAACACTGTAAAGGAATCATCATGTCCATCTATAGCAAAATGAACGTGAGCG  >  CP006881/2921960‑2922218                                                                                                                             |                                                                                                                                       tACAGGATTGATCGCATACGCTCCTGTAAATACGCCTGTCTTTGTTTTCGCAAGATCTGTGCGTTCTAGGTCACTCTTTGATTGAATTTCTTTGATATAAGCTTCAACAGCTTCTTTTTGCTCTGCCGTTGTGATGtt                                                                                                                           <  1:8665/138‑1 (MQ=255)        ttGATCGCATACGCTCCTGTAAATACGCCTGTCTTTGTTTTCGCAAGATCTGTGCGTTCTAGGTCACTCTTTGATTGAATTTCTTTGATATAAGCTTCAACAGCTTCTTTTTGCTCTg                                                                                                                                        <  6:119872/118‑1 (MQ=255)        ttGATCGCATACGCTCCTGTAAATACGCCTGTCTTTGTTTTCGCAAGATCTGTGCGTTCTAGGTCACTCTTTGATTGAATTTCTTTGATATAAGCTTCAACAGCTTCTTTTTGCTCTg                                                                                                                                        >  5:119872/1‑118 (MQ=255)         tGATCGCATACGCTCCTGTAAATACGCCTGTCTTTGTTTTCGCAAGAGCTGTGCGTTCTAGGTCACTCTTTGATTGAATTTCTTTGATATAAGCTTCAACAGCTTCTTTTTGCTCTGCCGTTGTGATGTTTTccacca                                                                                                                   >  1:284532/1‑138 (MQ=255)           aTCGCATACGCTCCTGTAAATACGCCTGTCTTTGTTTTCGCAAGATCTGTGCGTTCTAGGTCACTCTTTGATTGAATTTCTTTGATATAAGCTTCAACAGCTTCTTTTTGCTCTGCCGTTGTGATGTTTTCCACCAATg                                                                                                                >  8:101059/1‑139 (MQ=255)           aTCGCATACGCTCCTGTAAATACGCCTGTCTTTGTTTTCGCAAGATCTGTGCGTTCTAGGTCACTCTTTGATTGAATTTCTTTGATATAAGCTTCAACAGCTTCTTTTTGCTCTGCCGTTGTGATGTTTTCCACCAATg                                                                                                                >  5:203915/1‑139 (MQ=255)           aTCGCATACGCTCCTGTAAATACGCCTGTCTTTGTTTTCGCAAGATCTGTGCGTTCTAGGTCACTCTTTGATTGAATTTCTTTGATATAAGCTTCAACAGCTTCTTTTTGCTCTGCCGTTGTGATGTATTCCACCaagg                                                                                                                >  8:91126/1‑137 (MQ=255)              gCATACGCTCCTGTAAATACGCCTGTCTTTGTTTTCGCAAGATCTGTGCGTTCTAGGTCACTCTTTGATTGAATTTCTTTGATATAAGCTTCAACAGCTTCTTTTTGCTCTGCCGTTGTGATGTTTTCCACCAATGCg                                                                                                              <  5:205371/138‑1 (MQ=255)                aTACGCTCCTGTAAATACGCCTGTCTTTGTTTTCGCAAGATCTGTGCGTTCTAGGTCACTCTTTGATTGAATTTCTTTGATATAAGCTTCAACAGCTTCTTTTTGCTCTg                                                                                                                                        <  8:172697/110‑1 (MQ=255)                aTACGCTCCTGTAAATACGCCTGTCTTTGTTTTCGCAAGATCTGTGCGTTCTAGGTCACTCTTTGATTGAATTTCTTTGATATAAGCTTCAACAGCTTCTTTTTGCTCTg                                                                                                                                        >  7:172697/1‑110 (MQ=255)                aTACGCTCCTGTAAATACGCCTGTCTTTGTTTTCGCAAGATCTGTGCGTTCTAGGTCACTCTTTGATTGAATTTCTTTGATATAAGCTTCAACAGCTTCTTTTTGCTCTGCCg                                                                                                                                     <  7:270150/113‑1 (MQ=255)                aTACGCTCCTGTAAATACGCCTGTCTTTGTTTTCGCAAGATCTGTGCGTTCTAGGTCACTCTTTGATTGAATTTCTTTGATATAAGCTTCAACAGCTTCTTTTTGCTCTGCCg                                                                                                                                     >  8:270150/1‑113 (MQ=255)                   cGCTCCTGTAAATACGCCTGTCTTTGTTTTCGCAAGATCTGTGCGTTCTAGGTCACTCTTTGATTGAATTTCTTTGATATAAGCTTCAACAGCTTCTTTTTGCTCTGCCGTTGTGATGTTTTCCACCAATGCGTGTTcc                                                                                                        <  1:263584/139‑1 (MQ=255)                   cGCTCCTGTAAATACGCCTGTCTTTGTTTTCGCAAGATCTGTGCGTTCTAGGTCACTCTTTGATTGAATTTCTTTGATATAAGCTTCAACAGCGTCTTTTTGCTCTGCCGTTGTGATGTTTTCCACCAATGCGTGTTcc                                                                                                        <  7:91126/139‑1 (MQ=255)                   cGCTCCTGTAAATACGCCTGTCTCTGTTTGCGCAAGATCTGTGCGTTCTAGGTCACTCTTTGATTGAATTGCATTGATATAAGCTTCAACAGCTTCTTTTTGCTCTGCCGTTGTGATGTTTTCCACCAATGCGGGTTcc                                                                                                        <  4:172768/139‑1 (MQ=255)                   cGCTCCTGTAAATACGCCTGGCTTTGTTTTCGCAAGATCTGTGCGTTCTAGGTCACTCTTTGATTGAATTTCTTTGATATAAGCTTCAACAGCTTCTTTTTGCTCTGCCGTTGTGATGTTTTCCACCAATGCGTGTTcc                                                                                                        <  6:113080/139‑1 (MQ=255)                          gTAAATACGCCTGTCTTTGTTTTCGCAAGATCTGTGAGTTCTAGGTCACTCTTTGATTGAATTTCTTTGATATAAGATTAAACAGCTTCTTTTTGCTCTGCCGTTGTGATGTTTTCAACCAATGCGTTTTCAGTGGc                                                                                                   >  6:175282/1‑137 (MQ=255)                             aaTACGCCTGTCTTTGTTTTCGCAAGATCTGTGCGTTCTAGGTCACTCTTTGATTGAATTTCTTTGATATAAGCTTCAACAGCTTCTTTTTGCTCTGCCGTTGTGATGTTTTCCACCAATGCGTGTTCCGGGGCAAgg                                                                                               <  7:101059/138‑1 (MQ=255)                             aaTACGCCTGTCTTTGTTTTCGCAAGATCTGTGCGTTCTAGGTCACTCTTTGATTGAATTTCTTTGATATAAGCTTCAACAGCTTCTTTTTGCTCTGCCGTTGTGATGTTTTCCACCAATGCGTGTTCCGGGGCAAgg                                                                                               <  2:55649/138‑1 (MQ=255)                             aaTACGCCTGTCTTTGTTTTCGCAAGATCTGTGCGTTCTAGGTCACTCTTTGATTGAATTTCTTTGATATAAGCTTCAACAGCTTCTTTTTGCTCTGCCGTTGTGATGTTTTCCACCAATGCGTGTTCCGGGGCAAgg                                                                                               <  4:42391/138‑1 (MQ=255)                             aaTACGCCTGTCTTTGTTTTCGCAAGATCTGTGCGTTCTAGGTCACTCTTTGATTGAATTTCTTTGATATAAGCTTCAACAGCTTCTTTTTGCTCTGCCGTTGTGATGTTTTCCACCAATGCGTGTTCCGGGGCAAgg                                                                                               <  1:116349/138‑1 (MQ=255)                             aaTACGCCTGTCTTTGTTTTCGCAAGATCTGTGCGTTCTAGGTCACTCTTTGATTGAATTTCTTTGATATAAGCTTCAACAGCTTCTTTTTGCTCTGCCGTTGTGATGTTTTCCACCAATGCGTGTTCCGGGGCAAGGa                                                                                              <  8:171494/139‑1 (MQ=255)                             aaTACGCCTGTCTTTGTTTTCGCAAGATCTGTGCGTTCTAGGTCACTCTTTGATTGAATTTCTTTGATATAAGCTTCAACAGCTTCTTTTTGCTCTGCCGTTGTGATGTTTTCCACCAATGCGTGTTCCGGGGCAAGGa                                                                                              <  8:267316/139‑1 (MQ=255)                             aaTACGCCTGTCTTTGTTTTCGCAAGATCTGTGCGTTCTAGGTCACTCTTTGATTGAATTTCTTTGATATAAGCTTCAACAGCTTCTTTTTGCTCTACCGTTGTGATGTTTTCCACCAATGCGTGTTCCGGGGCAAGGa                                                                                              >  8:73197/1‑139 (MQ=255)                             aaTACGCCTGTCTTTGTTTTCGCAAGATCTGTGCGTTCTAGGTCACTCTTTGATTGAATTTCTTTGATATAAGCTTCAACAGCTTCTTTTTGCTCTACCGTTGTGATGTTTTCCACCAATGCGTGTTCCGGGGCAAGGa                                                                                              >  8:77479/1‑139 (MQ=255)                              aTACGCCTGTCTTTGTTTTCGCAAGATCTGTGCGTTCTAGGTCACTCTTTGATTGAATTTCTTTGATATAAGCTTCAACAGCTTCTTTTTGCTCTGCCGTTGTGATGTTTTCCACCAATGCGTGTTCCGGGGCAAGGAc                                                                                             <  3:238199/139‑1 (MQ=255)                               tACGCCTGTCTTTGTTTTCGCAAGATCTGTGCGTTCTAGGTCACTCTTTGATTGAATTTCTTTGATATAAGCTTCAACAGCTTCTTTTTGCTCTGCCGTTGTGATGTTTTCCACCAATGCGTGGTCCGGGGCAAGGACa                                                                                            <  4:88877/139‑1 (MQ=255)                                aCGCCTGTCTTTGTTTTCGCAAGATCTGTGCGTTCTAGGTCACTCTTTGATTGAATTTCTTTGATATAAGCTTCAACAGCTTCTTTTTGCTCTGCCGTTGTGATGTTTTCCACCAATGCGTGTTCCGGGGCAAGGACAg                                                                                           >  1:173708/1‑139 (MQ=255)                                aCGCCTGTCTTTGTTTTCGCAAGATCTGTGCGTTCTAGGTCACTCTTTGATTGAATTTCTTTGATATAAGCTTCAACAGCTTCTTTTTGCTCTGCCGTTGTGATGTTTTCCACCAATGCGTGTTCCGGGGCAAGGACAg                                                                                           >  5:276128/1‑139 (MQ=255)                                  gCCTGTCTTTGTTTTCGCAAGATCTGTGCGTTCTAGGTCACTCTTTGATTGAATTTCTTTGATATAAGCTTCAACAGCTTCTTTTTGCTCTGCCGTTGTGATGTTTTCCACCAATGCGTGTTCCGGGGCAAGGACAgtg                                                                                         >  8:264527/1‑139 (MQ=255)                                  gCCTGTCTTTGTTTTCGCAAGATCTGTGCGTTCTAGGTCACTCTTTGATTGAATTTCTTTGATATAAGCTTCAACAGCTTCTTTTTGCTCTGCCGTTGTGATGTTTTCCACCAATGCGTGTTCCGGGGCAAGGACAgtg                                                                                         >  5:169345/1‑139 (MQ=255)                                  gCCTGTCTTTGTTTTCGCAAGATCTGTGCGTTCTAGGTCACTCTTTGATTGAATTTCTTTGATATAAGCTTCAACAGCTTCTTTTTGCTCTGCCGTTGTGATGTTTTCCACCAATGCGTGTTCCGGGGCAAGGACAgtg                                                                                         >  5:126280/1‑139 (MQ=255)                                       tCTTTGTTTTCGCAAGATCTGTGCGTTCTAGGTCACTCTTTGATTGAATTTCTTTGATATAAGCTTCAACAGCTTCTTTTTGCTCTACCGTTGTGATGTTTTCCACCAATGCGTGTTCCGGGGCAAGGACAGtgtatg                                                                                     <  7:77479/138‑1 (MQ=255)                                       tCTTTGTTTTCGCAAGATCTGTGCGTTCTAGGTCACTCTTTGATTGAATTTCTTTGATATAAGCTTCAACAGCTTCTTTTTGCTCTACCGTTGTGATGTTTTCCACCAATGCGTGTTCCGGGGCAAGGACAGtgtatg                                                                                     <  7:73197/138‑1 (MQ=255)                                           tGTTTTCGCAAGATCTGTGAGATCTATGTCACTCTTTGATTGAATTTCTTTGATATAAGCTTCAACAGCTTCTTTTTGCTCTGCCGTTGTGATGTTTTCCACCAATGCGTGTTCCGGGGCAAGGACAGtgtatgta                                                                                   <  6:249240/136‑1 (MQ=255)                                                 ctcAAGATCTGTGCGTTCTAGGTCACTCTTTGATTGAATTTCTTTGATATAATCTTCAAAATATTATTTTTTATATGACTTTGTGATGTTTTAAACAAATGCGTGTTCCTGGGCAAGGACAGTTTATGTAGCGACaaa                                                                           >  4:148828/3‑138 (MQ=255)                                                 cGCAAGATCTGTGCGTTCTAGGTCACTCTTTGATTGAATTTCTTTGATATAAGCTTCAACAGCTTCTTTTTGCTCTGCCGTTGTGATGTTTTCCACCAATGCGTGTTCCGGGGc                                                                                                   <  8:38074/114‑1 (MQ=255)                                                 cGCAAGATCTGTGCGTTCTAGGTCACTCTTTGATTGAATTTCTTTGATATAAGCTTCAACAGCTTCTTTTTGCTCTGCCGTTGTGATGTTTTCCACCAATGCGTGTTCCGGGGc                                                                                                   >  7:38074/1‑114 (MQ=255)                                                            tgCGTTCTAGGTCACTCTTTGATTGAATTTCTTTGATATAAGCTTCAACAGCTTCTTTTTGCTCTGCCGTTGTGATGTTTTCCACCAATGCGTGTTCCGGGGCAAGGACAGTGTATGTAGCGCCAAACAGCGTATc                                                                  <  2:48907/136‑1 (MQ=255)                                                            tgCGTTCTAGGTCACTCTTTGATTGAATTTCTTTGATATAAGCTTCAACAGCTTCTTTTTGCTCTGCCGTTGTGATGTTTTCCACCAATGCGTGTTCCGGGGCAAGGACAGTGTATGTAGCGCCAAACAGCGTATCTgg                                                               >  1:48907/1‑139 (MQ=255)                                                              cGTTCTAGGTCACTCTTTGATTGAATTTCTTTGATATAAGCTTCAACAGCTTCTTTTTGCTCTGCCGTTGTGATGTTTTCCACCAATGCGTGTTCCGGGGCAAGGACAGTGTATGTAGCGCCAAACAGCGTATCTGGTc                                                             <  6:276128/139‑1 (MQ=255)                                                                ttCTAGGTCACTCTTTGATTGAATTTCTTTGATATAAGCTTCAACAGCTTCTTTTTGCTCTGCCGTTGTGATGTTTTCCACCAATGCGTGTTCCGGGGCAAGGACAGTGTATGTAGCGCCAAACAGCGTATCTGGTCtt                                                           <  6:203915/139‑1 (MQ=255)                                                                ttCTAGGTCACTCTTTGATTGAATTTCTTTGATATAAGCTTCAACAGCTTCTTTTTGCTCTGCCGTTGTGATGTTTTCCACCAATGCGTGTTCCGGGGCAAGGACAGTGTATGTAGCGCCAAACAGCGTATCTGGTCtt                                                           >  2:76648/1‑139 (MQ=255)                                                                 tCTAGGTCACTCTTTGATTGAATTTCTTTGATATAAGCTTCAACAGCTTCTTTTTGCTCTGCCGTTGTGATGTTTTCCACCAATGCGTGTTCCGGGGCAAGGACAGTGTATGTAGCGCCAAACAGCGTATCTGGTCttg                                                          >  8:45940/1‑139 (MQ=255)                                                                  cTAGGTCACTCTTTGATTGAATTTCTTTGATATAAGCTTCAACAGCTTCTTTTTGCTCTGCCGTTGTGATGTTTTCCACCAATGCGTGTTCCGGGGCAAGGAc                                                                                             <  7:201191/103‑1 (MQ=255)                                                                  cTAGGTCACTCTTTGATTGAATTTCTTTGATATAAGCTTCAACAGCTTCTTTTTGCTCTGCCGTTGTGATGTTTTCCACCAATGCGTGTTCCGGGGCAAGGACAGTGTATGTAGCGCCAAACAGCGTATCTGGTCttgt                                                         >  4:121961/1‑139 (MQ=255)                                                                  cTAGGTCACTCTTTGATTGAATTTCTTTGATATAAGCTTCAACAGCTTCTTTTTGCTCTGCCGTTGTGATGTTTTCCACCAATGCGTGTTCCGGGGCAAGGACAGTGTATGTAGCGCCAAACAGCGTATCTGGTCttgt                                                         >  2:243966/1‑139 (MQ=255)                                                                  cTAGGTCACTCTTTGATTGAATTTCTTTGATATAAGCTTCAACAGCTTCTTTTTGCTCTCCCGTTGTGATGTTTTCCACCAATGCGTGTTCCGGGGCAAGGAc                                                                                             >  8:201191/1‑103 (MQ=255)                                                                        cACTCTTTGATTGAATTTCTTTGATATAATCTTCAACAGCTTCTTTTTGCTCTGCCGTTGTGATGTTTTCCACCAATGCGTGTTCCGGGGCAAGGACAGTGTATGTAGCGCCAAACAGCGTATCTGGTCTTGTTGTaaa                                                   <  3:148828/139‑1 (MQ=255)                                                                         aCTCTTTTATTGAATTTCTTTGATATAAGCTTCAACAGATTCTTTTTGCTCTGCCGTTGTGATGTTTTCCACCAATGCGTGTTCCGGGGCAAGGACAGTGTATGTAGCGCCAAACAGCGTATCTGGTCTTGTTGTaaa                                                   <  5:175282/138‑1 (MQ=255)                                                                         aCTCTTTGATTGAATTTCTTTGATATAAGCTTCAACAGCTTCTTTTTGCTCTGCCGTTGTGATGTTTTCCACCAATGCGTGTTCCGGGGCAAGGACAGTGTATGTAGCGCCAAACAGCGTATCTGGTCTTGTTGTaaa                                                   <  1:29596/138‑1 (MQ=255)                                                                          ctctTTGATTGAATTTGTTTGATATAAGCTTCAACAGCTTCTTTTTGCTCTGCCGTTGTGATGTTTTCCACCAATGCGTGTTCCGGGGCAAGGACAGTGTATGTAGCGCCAAACAGCGTATCTGGTCTTGTTGTAAac                                                  >  1:5360/1‑138 (MQ=255)                                                                          ctctTTGATTGAATTTCTTTGATATAAGCTTCAACAGCTTCTTTTTGCTCTGCCGTTGTGATGTTTTCCACCAATGCGTGTTCCGGGGCAAGGACAGTGTATGTAGCGCCAAACAGCGTATCTGGTCTTGTTGTAAaca                                                 <  1:243966/139‑1 (MQ=255)                                                                          ctctTTGATTGAATTTCTTTGATATAAGCTTCAACAGCTTCTTTTTGCTCTGCCGTTGTGATGTTTTCCACCAATGCGTGTTCCGGGGCAAGGACAGTGTATGTAGCGCCAAACAGCGTATCTGGTCTTGTTGTAAaca                                                 <  3:121961/139‑1 (MQ=255)                                                                                 attgaATTTCTATGATATAAGCTTCAACAGCTTCTTTTTGCTCTGCCGTTGTGATGTTTTCCACCAATGCGTGTTCCGGGGCAAGGACAGTGTATGTAGCGCCAAACAGCGTATCTGGTCTTGTTGTAAACACTGTaa                                           <  7:98701/138‑1 (MQ=255)                                                                                     aaTTTCTTTGATATAAGCTTCAACAGCTTCTTTTTGCTCTGCCGTTGTGATGTTTTCCACCAATGCGTGTTCCGGGGc                                                                                                   <  8:121963/78‑1 (MQ=255)                                                                                     aaTTTCTTTGATATAAGCTTCAACAGCTTCTTTTTGCTCTGCCGTTGTGATGTTTTCCACCAATGCGTGTTCCGGGGc                                                                                                   >  7:121963/1‑78 (MQ=255)                                                                                     aaTTTCTTTGATATAAGCTTCAACAGCTTCTTTTTGCTCTGCCGTTGTGATGTTTTCCACCAATGCGTGTTCCGGGGCAAgg                                                                                               <  7:85326/82‑1 (MQ=255)                                                                                     aaTTTCTTTGATATAAGCTTCAACAGCTTCTTTTTGCTCTGCCGTTGTGATGTTTTCCACCAATGCGTGTTCCGGGGCAAgg                                                                                               >  8:85326/1‑82 (MQ=255)                                                                                     aaTTTCTTTGATATAAGCTTCAACAGCTTCTTTTTGCTCTGCCGTTGTGATGTTTTCCACCAATGCGTGTTCCGGGGCAAGGACAGTGTATGTAGCGCCAAACAGCGTATCTGGTCTTGTTGTAAACACTGTAAAGGaa                                      <  2:173708/139‑1 (MQ=255)                                                                                       tttCTTTGATATAAGCTTCAACAGCTTCTTTTTGCTCTGCCGTTGTGATGTTTTCCACCAATGCGTGTTCCGGGGCAAGGACAGTGTATGTAGCGCCAAACAGCGTATCGGGTCTTGTTGTAAACACTGTAAAGGAat                                     >  6:64794/1‑138 (MQ=255)                                                                                           tttGATATAAGCTTCAACAGCTTCTTTTTGCTCTGCCGTTGTGATGTTTTCCACCAATGCGTGTTCCGGGGCAAGGACAGTGTATGTAGCGCCAAACAGCGTATCTGGTCTTGTTGTaa                                                    <  5:282679/119‑1 (MQ=255)                                                                                           tttGATATAAGCTTCAACAGCTTCTTTTTGCTCTGCCGTTGTGATGTTTTCCACCAATGCGTGTTCCGGGGCAAGGACAGTGTATGTAGCGCCAAACAGCGTATCTGGTCTTGTTGTaa                                                    >  6:282679/1‑119 (MQ=255)                                                                                               atatAAGCTTCAACAGCTTCTTTTTGCTCTGCCGTTGTGATGTTTTCCACCAATGCGTGTTCCGGGGCAAgg                                                                                               <  4:115878/72‑1 (MQ=255)                                                                                               atatAAGCTTCAACAGCTTCTTTTTGCTCTGCCGTTGTGATGTTTTCCACCAATGCGTGTTCCGGGGCAAgg                                                                                               >  3:115878/1‑72 (MQ=255)                                                                                                               cttcttTTTGCTCTGCCGTTGTGATGTTTTCCACCAATGCGTGTTcc                                                                                                        >  2:120194/1‑47 (MQ=255)                                                                                                               cttcttTTTGCTCTGCCGTTGTGATGTTTTCCACCAATGCGTGTTcc                                                                                                        <  1:120194/47‑1 (MQ=255)                                                                                                               cttcttTTTGCTCTGCCGTTGTGATGTTTTCCACCAATGCGTGTTCCGGGGCAAGGACAGTGTATGTAGCTCCAAACAGCGTATCTTGTCTTGTTGTAAACACTGTAAAGGAATCATCATGTCCATCTATAGCAAAAt             >  5:219913/1‑138 (MQ=255)                                                                                                                         ctctGCCGTTGTGATGTTTTCCACCAATGCGTGTTCCGGGGCAAGGACAGTGTATGTAGCGCCAAACAGCGTATCTGGTCTTGTTGTAAACACTGTAAAGGACTCATCATGTCCATCTATAGCAAAATGAACGGGAgcg  >  6:163900/1‑139 (MQ=255)                                                                                                                         ctctGCCGTTGTGATGTTTTCCACCAATGCGTGTTCCGGGGCAAGGACAGTGTATGTAGCGCCAAACAGCGTATCTGGTCTTGTTGTAAACACTGTAAAGGAATCATCATGTCCATCTATAGCAAAATGAACGTGAgcg  >  4:10401/1‑139 (MQ=255)                                                                                                                             |                                                                                                                                       TACAGGATTGATCGCATACGCTCCTGTAAATACGCCTGTCTTTGTTTTCGCAAGATCTGTGCGTTCTAGGTCACTCTTTGATTGAATTTCTTTGATATAAGCTTCAACAGCTTCTTTTTGCTCTGCCGTTGTGATGTTTTCCACCAATGCGTGTTCCGGGGCAAGGACAGTGTATGTAGCGCCAAACAGCGTATCTGGTCTTGTTGTAAACACTGTAAAGGAATCATCATGTCCATCTATAGCAAAATGAACGTGAGCG  >  CP006881/2921960‑2922218

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑