| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP006881 | 2,963,948 | G→A | 100% | intergenic (‑135/+145) | U712_15285 ← / ← U712_15290 | Manganese‑binding lipoprotein mntA/o‑succinylbenzoate synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 2,963,948 | 0 | G | A | 97.4% | 116.3 / ‑5.4 | 37 | intergenic (‑135/+145) | U712_15285/U712_15290 | Manganese‑binding lipoprotein mntA/o‑succinylbenzoate synthase |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (33/2); total (34/3) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
ATATTTCTCCTCCTCTTTGCCATCAATGTCCGATTGTTTTATCTTATGTTGTCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCC‑TCATGCAAAATTATCGCGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACGAACACTTTTACTGTAACAGCACAGTGTCATTTATTCAAAA > CP006881/2963812‑2964074 | atatTTCTCCTCCTCTTTGCCATCAATGTCCGATTGTTTTATCTTATGTTGTCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCC‑TCATGCAAAATTATcacg > 4:293888/1‑139 (MQ=255)atatTTCTCCTCCTCTTTGCCATCAATGTCCGATTGTTTTATCTTATGTTGTCAGCTCTTAGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCC‑TCATGCAAAATTATcacg > 1:84297/1‑139 (MQ=255) ctcctcctcTTTGCCATCAATGTCCGATTGTTTTATCTTATGTTGTCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCC‑TCATGCAAAATTATCACGTTTGGc > 1:126210/1‑139 (MQ=255) ctcctcctcTTTGCCATCAATGTCCGATTGTTTTATCTTATGTTGTCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCC‑TCATGCAAAATTATCACGTTTGGc > 3:192550/1‑139 (MQ=255) ctcctcctcTTTGCCATCAATGTCCGATTGTTTTATCTTATGTTGTCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTT‑CC‑TCATGCAAAATTATAACGTTTTGc > 5:189661/1‑138 (MQ=255) tcctcTTTGCCATCAATGTCCGATTGTTTTATCTTATGTTGTCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCC‑TCATGCAAAATTATCACGTTTGGCCAtt > 7:82244/1‑139 (MQ=255) ctctTTGCCATCAATGTCCGATTGTTTTATCTTATGTTGTCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCC‑TCATGCAAAATTATCACGTTTGGCCAtttt > 3:101202/1‑139 (MQ=255) ctTTGCCATCAATGTCCGATTGTTTTATCTTATGTTGTCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCC‑TCATGCAAAATTATCACTTTTGGACATTTTTg > 2:14100/1‑139 (MQ=255) tGCCATCAATGTCCGATTGTTTTATCTTATGTTGTCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCC‑TCATGCAAAATTATCACGTTTGGCCATTTTTGTc > 7:95793/1‑138 (MQ=255) tGCCATCAATGTCCGATTGTTTTATCTTATGTTGTCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCC‑TCATGCAAAATTATCACGTTTGGCCATTTTTGTc > 3:87357/1‑138 (MQ=255) tCCGATTGTTTTATCTTATGTTGTCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCC‑TCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTGATTTa < 5:233828/138‑1 (MQ=255) tgttgtCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCC‑TCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTggg > 4:8892/1‑139 (MQ=255) tCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCC‑TCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGTTcag > 5:174120/1‑138 (MQ=255) tCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCC‑TCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTcagc > 2:145395/1‑139 (MQ=255) tCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCC‑TCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTcag > 3:36562/1‑138 (MQ=255) tCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCC‑TCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTcag > 3:289858/1‑138 (MQ=255) tCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCC‑TCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTcag > 5:147129/1‑138 (MQ=255) aGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCC‑TCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTcagcag > 2:144921/1‑139 (MQ=255) tgtgACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCC‑TCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCactc > 8:254141/1‑135 (MQ=255) tgtgACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCC‑TCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAAc > 5:8140/1‑138 (MQ=255) tgtgACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCC‑TCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAAc > 7:265754/1‑138 (MQ=255) tgtgACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCC‑TCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAAc > 6:26989/1‑138 (MQ=255) tgACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCC‑TCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAAc > 4:88539/1‑136 (MQ=255) aTTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCAC‑TCATGCAAAATTATCGCGTTTGGCCATTTTTGTCAATCTGATTTACATAAAAAAACCGTCTGGGTCAGCAGCCGTTCAATACGAGAAGGGATg > 8:253454/1‑138 (MQ=255) tGTTTTATTTACACAGGACAAAAAGTTTCCC‑TCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACGAACACt > 5:133769/1‑139 (MQ=255) tGTTTTATTTACACAGGACAAAAAGTTTCCC‑TCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACGAACACt > 5:201127/1‑139 (MQ=255) gTTTTATTTACACAGGACAAAAAGTTTCCC‑TCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACGAACACtt > 5:102529/1‑139 (MQ=255) atttaAACAGCAAAAAAATTTTCCC‑TAATACAAATTTATCAGTTTTGGCCATTTTTGTAAAGCTTATTTAAATAAAAAACCCGGCGGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACGAACACTTTTAc < 8:95793/138‑1 (MQ=255) ggACAAAAAGTTTCCC‑TCATGCAAAATTATCACGTTTGGCCAGTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTATTCCACGAACACTTTTACTGTAACAGca > 8:182288/1‑139 (MQ=255) gACAAAAAGTTTCCC‑TCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACGAACACTTTTACTGTAACAGca > 8:67168/1‑138 (MQ=255) aaaaTTTTCCCATCATCAAAAATTATCTGTTTTGGCAATTTTTGTCATGCTTATTAAAAAAAAAACCCCGGGGGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACGAACACTTTTACTGTAACAGCACAgt < 6:102529/139‑1 (MQ=255) aaaGTTTCCC‑TCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACGAACACTTTTACTGTAACAGCACAgtgt > 6:252365/1‑139 (MQ=255) ttCCC‑TCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACGAACACTTTTACTGTAACAGCACAGTGTctgtc > 5:120531/1‑135 (MQ=255) ttCCC‑TCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACGAACACTTTTACTGTAACAGCACAGTGTCAttt > 2:267075/1‑139 (MQ=255) ttCCC‑TCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACGAACACTTTTACTGTAACAGCACAGTGTCAtt > 5:87866/1‑138 (MQ=255) ttCCC‑TCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACGAACACTTTTACTGTAACAGCACAGTGTCAtt > 2:157111/1‑138 (MQ=255) taccc‑TCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCAGATTTACATAAAAAAACAGGCTGTGTCAGCACCCGGTCCAAACGAGAAGGGATCTATTCAACGAACACTTTTACTGTAACAGAACAGTGTCAtt > 1:136580/3‑138 (MQ=255) aTGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACGAACACTTTTACTGTAACAGCACAGTGTCATTTATTCaa > 3:30643/1‑138 (MQ=255) tGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACGAACACTTTTACTGTAACAGCACAGTGTCATTTATTCaaaa > 6:14976/1‑139 (MQ=255) | ATATTTCTCCTCCTCTTTGCCATCAATGTCCGATTGTTTTATCTTATGTTGTCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCC‑TCATGCAAAATTATCGCGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACGAACACTTTTACTGTAACAGCACAGTGTCATTTATTCAAAA > CP006881/2963812‑2964074 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |