| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP006881 | 3,217,386 | A→C | 28.3% | F277V (TTT→GTT) | U712_16570 ← | putative MFS‑type transporter yvqJ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 3,217,386 | 0 | A | C | 28.3% | 34.4 / 11.0 | 28 | F277V (TTT→GTT) | U712_16570 | putative MFS‑type transporter yvqJ |
| Reads supporting (aligned to +/- strand): ref base A (18/2); new base C (2/6); total (20/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.76e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGAAAAAGACGAGCGGCAAAAAGGTATTCATCCCGGTAATAAAGAAAGCAAATCCTTCAATAATGCCGGCAGTGACGAATAACAAGCCGTACCTGTTTACCTTGACTTTCGCCAGCACAAACCCTAATAAAAA‑CGCGCCCGCGGCGGTGGTGGCCTTTAAAAGCGAGTATACGATCGGCTGGCCGTTTAAATCTTCCGCAACGTATACGGCAGAGAGCGCTTCCCATGGGGCAGCCGCGAAATTCATAAAGATACAGTAAATGGCGAGCGG > CP006881/3217254‑3217524 | cGAAAAAGACGAGCGGCAAAAAGGTATTCATCCCGGTAATAAAGAAAGCAAATCCTTCAATAATGCCGGCAGTGACGAATAACAAGCCGTACCTGTTTACCTTGACTTTCGCCAGCACAAACCCTAATAAAAA‑CGCGcc > 3:7424/1‑139 (MQ=255) gAGCGGCAAAAAGGTATTCATCCCGGTAATAAAGAAAGCAAATCCTTCAATAATGCCGGCAGTGACGAATAACAAGCCGTACCTGTTTACCTTGACTTTCGCCAGCACAAACCCTAATAAAAC‑CGCGCCCGCGGCggtg > 1:204963/1‑139 (MQ=255) gAGCGGCAAAAAGGTATTCATCCCGGTAATAAAGAAAGCAAATCCTTCAATAATGCCGGCAGTGACGAATAACAAGCCGTACCTGTTTACCTTGACTTTCGCCAGCACAAACCCTAATAAAAA‑CGCGCCCGCGGCggtg > 1:181754/1‑139 (MQ=255) ttCATCCCGGTAATAAAGAAAGCAAATCCTTCAATAATGCCGGCAGTGACGAATAACAAGCCGTACCTGTTTACCTTGACTTTCGCCAGCACAAACCCTAATAAAAA‑CGCGCCCGCGGCGGTGGTGGCCTTTAAAAGCg > 3:170645/1‑139 (MQ=255) cccGGTAATAAAGAAAGCAAATCCTTCAATAATGCCGGCAGTGACGAATAACAAGCCGTACCTGTTTACCTTGACTTTCGCCAGCACAACCCCTAATAAAAA‑CGCGCCCGCGGCGGTGGTGGCCTTTAAAAGCGAGta > 4:163164/1‑138 (MQ=255) ggTAATAAAGAAAGCAAATCCTTCAATAATGCCGGCAGTGACGAATAACAAGCCGTACCTGTTTACCTTGACTTTCGCCAGCACAAACCCTAATAAAAA‑CGCGCCCGCGGCGGTGGTGGCCTTTAAAAGCGAGTATAc > 5:225380/1‑138 (MQ=255) ggTAATAAAGAAAGCAAATCCTTCAATAATGCCGGCAGTGACGAATAACAAGCCGTACCTGTTTACCTTGACTTTCGCCAGCACAAACCCTAATAAAAA‑CGCGCCCGCGGCGGTGGTGGCCTTTAAAAGCGAGTATACg > 1:199008/1‑139 (MQ=255) gaacccAAACCCTTCAAAAATCCCGGCTGTCACGAAAAACACCCCGAACCTGTTAACCTTCAGTTGCGCCAGCAAAACCCATAATAAACC‑CGCGCCCGCGGCGGTGGTGGCCTTTAAAAGCGAGTATACGATCGGCTgg < 7:235687/134‑1 (MQ=255) agCAAATCCTTCAATAATGCCGGCAGTGACGAATAACAAGCCGTCCCTGTTTACCTTGACTTTCGCCAGCACAACCCCTAATAAAAA‑CGCGCCCGCGGCGGTGGTGGCCTTTAAAAGCGAGTATACGATCGGCTGGCCg > 1:263947/1‑139 (MQ=255) agCAAATCCTTCAATAATGCCGGCAGTGACGAATAACAAGCCGTACCTGTTTACCTTGACTTTCGCCAGCACAAACCCTAATAAAAA‑CGCGCCCGCGGCGGTGGTGGCCTTTAAAAGCGAGTATACGATCGGCTGGCCg > 3:6268/1‑139 (MQ=255) agCAAATCCTTCAATAATGCCGGCAGTGACGAATAACAAGCCGTACCTGTTTACCTTGACTTTCGCCAGCACAAACCCTAATAAAAA‑CGCGCCCGCGGCGGTGGTGGCCTTTAAAAGCGAGTATACGATCGGCTGGCCg > 7:284011/1‑139 (MQ=255) aTCCTTCAATAATGCCGGCAGTGACGAATAACAAGCCGTACCTGTTTACCTTGACTTTCGCCAGCACAAACCCTAATAAAAA‑CGCGCCCGCGGCGGTGGTGTCCTTTAAAAGCGTGTATACGATCGGCTGGACGTTTa > 5:292510/1‑138 (MQ=255) aTCCTTCAATAATGCCGGCAGTGACGAATAACAAGCCGTACCTGTTTACCTTGACTTTCGCCAGCACAAACCCTAATAAAAA‑CGCGCCCGCGGCGGTGGTGGCCTTTAAAAGCGAGTATACGATCGGCTGGCCGTTTa > 7:1279/1‑138 (MQ=255) cAATAATGCCGGCAGTGACGAATAACAAGCCGTACCTGTTTACCTTGACTTTCGCCAGCACAAACCCTAATAAAAA‑CGCGCCCGCGGCGGTGGTGGCCTTTAAAAGCGAGTATAAGATCGGCTGGCCGTTTAAATCTTc > 4:35245/1‑139 (MQ=255) aatGCCGGCTGTCACAAAAAAAATGCCGAACCTTTTAACCTTGATTTCCCCAACCACAAACCAAAAAAAAAC‑CGCGCCCGCGGCGGTGGTGGCCTTTAAAAGCGAGTATACGATCGGCTGGCCGTTTAAATCTTCCGCa < 8:100754/139‑1 (MQ=255) aatCCCGCCAGTCACCAAAAACACCCCGTACCTGTTAACCTTGCCTTGC‑CCCCCACAAACCCAAAAAAAAACCGCGCCCGCGGCGGTGGTGGCCTTTAAAAGCGAGTATACGATCGGCTGGCCGTTTAAATCTTCCGCa < 7:243999/139‑1 (MQ=255) tGCCGGCAGTGACGAATAACAAGCCGTACCTGTTTACCTTGACTTTCGCCAGCACAAACCCTAATAAAAA‑CGCGCCCGCGGCGGTGGTGGCCTTTAAAAGCGAGTATACGATCGGCTGGCCGTTTAAATCTTCCGCAAc > 6:277318/1‑139 (MQ=255) ccGGCAGTGACGAATAACAAGCCGTACCTGTTTACCTTGACTTTCGCCAGCACAAACCCTAATAAAAA‑CGCGCCCGCGGCGGTGGTGGCCTTTAAAAGCGAGTATACGATCGGCTGGCCGTTTAAATCTTCCGCAACg > 4:230824/1‑138 (MQ=255) tAACAAGCCGTACCTGTTTACCTTGACTTTCGCCAGCACAAACCCTAATAAAAA‑CGCGCCCGCGGCGGTGGTGGCCTTTAAAAGCCAGGATAAGATCGGCTGGCCGTTTATATCTTACGGAAACTATCAGGCagagacc > 2:270645/1‑137 (MQ=255) atttttATCTTGACTTCCCCCCCAACAACCCAAAATAAACC‑CCCGCCCGCGGCGGTGGTGGCCTTTAAAAGCGAGTATACGATCGGCTGGCCGTTTAAATCTTCCGCAACGTATACGGCAGAGAGCGCTTCCCATgggg < 3:84488/136‑1 (MQ=255) tttACCTTGACTTTCGCCAGCACAAACCCTAATAAAAA‑CGCGCCCGCGGCGGTGGTGGCCTTTAAAAGCGAGTATACGATCGGCTGGCCGTTTAAATCTTCCGCAACGTATACGGCAGAGAGCGCTTCCCATGGGGCAg > 5:121726/1‑139 (MQ=255) tGACTTTCGCCAGCACAAACCCTAATAAAAA‑CGCGCCCGCGGCGGTGGTGGCCTTTAAAAGCGAGTATACGATCGGCTGGCCGTTTAAATCTTCCGCAACGTATACGGCAGAGAGCGCTTCCCATGGGGCAGCCGCGa > 6:128529/1‑138 (MQ=255) aCTTGCGCCACAACAAACCATAAAAAAAC‑CGCGCCCGCGGCGGTGGTGGCCTTTAAAAGCGAGTATACGATCGGCTGGCCGTTTAAATCTTCCGCAACGTATACGGCAGAGAGCGCTTCCCATGGGGCAGCCGCGaaa < 6:121726/138‑1 (MQ=255) cccaccaAAAACCAAAAAAAACC‑CGCGCCCGCGGCGGTGGTGGCCTTTAAAAGCGAGTATACGATCGGCTGGCCGTTTAAATCTTCCGCAACGTATACGGCAGAGAGCGCTTCCCATGGGGCAGCCGCGAAATTCATaa < 4:170645/138‑1 (MQ=255) ccaccccaAACCCAAAAAAAAACCGCGCCCGCGGCGGTGGTGGCCTTTAAAAGCGAGTATACGATCGGCTGGCCGTTTAAATCTTCCGCAACGTATACGGCAGAGAGCGCTTCCCATGGGGCAGCCGCGAAATTCATaa < 6:54883/133‑1 (MQ=255) aataaAAC‑CGCGCCCGCGGCGGTGGTGGCCTTTAAAAGCGAGTATACGATCGGCTGGCCGTTTAAATCTTCCGCAACGTATACGGCAGAGAgcgc > 8:135680/1‑95 (MQ=255) aataaAAC‑CGCGCCCGCGGCGGTGGTGGCCTTTAAAAGCGAGTATACGATCGGCTGGCCGTTTAAATCTTCCGCAACGTATACGGCAGAGAgcgc < 7:135680/95‑1 (MQ=255) aataaAAA‑CGCGCCCGCGGCGGTGGTGGCCTTTAAAAGCGAGTATACGATCGGCTGGCCGTTTAAATCTTCCGCAACGTATACGGCAGAGAGCGCTTCCCATGGGGCAGCCGCGAAATTCATAAAGATACAGTAAATgg > 5:71390/1‑139 (MQ=255) a‑CGCGCCCGCGGCGGTGGTGGCCTTTAAAAGCGAGTATACGATCGGCTGGCCGTTTAAATCTTCCGCAACGGATACGGCAGAGAGCGCTTCCCATGTGGCAGCCGCGAAAATAATAAATATACAGTAAATGGCGAGCgg > 5:221637/1‑139 (MQ=255) | CGAAAAAGACGAGCGGCAAAAAGGTATTCATCCCGGTAATAAAGAAAGCAAATCCTTCAATAATGCCGGCAGTGACGAATAACAAGCCGTACCTGTTTACCTTGACTTTCGCCAGCACAAACCCTAATAAAAA‑CGCGCCCGCGGCGGTGGTGGCCTTTAAAAGCGAGTATACGATCGGCTGGCCGTTTAAATCTTCCGCAACGTATACGGCAGAGAGCGCTTCCCATGGGGCAGCCGCGAAATTCATAAAGATACAGTAAATGGCGAGCGG > CP006881/3217254‑3217524 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |