Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 3,591,188 A→C 22.1% R6R (CGT→CGG)  U712_18465 ← Molybdenum cofactor biosynthesis protein A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068813,591,1880AC22.1% 51.2 / 2.5 34R6R (CGT→CGG) U712_18465Molybdenum cofactor biosynthesis protein A
Reads supporting (aligned to +/- strand):  ref base A (23/3);  new base C (2/6);  total (25/9)
Fisher's exact test for biased strand distribution p-value = 1.44e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTCCTCTTTCTTTAAAAAAGGATAATCCGGGCCGAATAATTCGGCGGGCATACAGTACGTGCATCGGAAGTTGCAGCGGTCTGTAACAGAAATGCGAAGGTCTCTCAGCGGTCTGTTTCTTTTATCCAACATCTGACGCGACTCTGTAATCATAGCTACAGCCCCTTCCTACTCCCTGATTCTCTCAGGGTGTGTATATACATTAAATGATCCGTTTCTGACAAATCCAATCGCTGTGATATTCAATTCTTCAGCCATTTGAATCGCAAGCTCC  >  CP006881/3591053‑3591326
                                                                                                                                       |                                                                                                                                          
ctcctcTTTCTTTAAAAAAGGATAATCCGGGCCGAATAATTCGGCGGGCATACAGTACGTGCATCGGAAGTTGCAGCTGTCTGTAACAGAAATGAGAAGGTCTCTCAGCGGTATGTTTCTTTTATCCAACATCTGAcgc                                                                                                                                         >  3:135394/1‑139 (MQ=255)
ctcctcTTTCTTTAAAAAAGGATAATCCGGGCCGAATAATTCGGCGGGCATACAGTACGTGCATCGGAAGTTGCAGCGGTCTGTAACAGAAATGCGAAGGTCTCTCAGCGGTCTGTTTCTTTTATCCAACATCTGAcgc                                                                                                                                         >  7:60924/1‑139 (MQ=255)
     ctttctttAAAAAAGGATAATCCGGGCCGAATAATTCGGCGGGCATACAGTACGTGCATCGGAAGTTTCAGCGGTCTGTAACAGAAATGCGAAGGTCTCTCAGCGGTCTGTTTCTTTTAACCAAAATCTTAAGCGActc                                                                                                                                    >  1:286805/1‑139 (MQ=255)
        tctttAAAAAAGGATAATCCGGGCCGAATAATTCGGCGGGCATACAGTACGTGCATCGGAAGTTGCAGCGGTCTGTAACAGAAATGCGAAGGTCTCTCAGCGGTCTGTTTCTTTTATCCAACATCTGACGCGACTCTg                                                                                                                                  >  1:130965/1‑138 (MQ=255)
                    gATAATCCGGGCCGAATAATTCGGCGGGCATACAGTACGTGCATCGGAAGTTGCAGCGGTCTGTAACAGAAATGCGAAGGTCTCTCAGCGGTCTGTTTCTTTTATCCAACATCTGACGCGACTCTGTAATCATAGCTa                                                                                                                      >  5:171901/1‑138 (MQ=255)
                        agccGTGCCTAATAATGGGGCGGCCATACAGTACGTGCATCGAATGTGGCCGGGGTCTTAAACACAAATCCCAAGGTCTCTCCGCGGTCTGTTTCTTTAAACAAAAATCCCCCGCGACTCTGTAATCATAGCTACAGcc                                                                                                                 <  4:140298/137‑1 (MQ=255)
                                ctaatattGCGGGGGGCATACCGTACTGGCATCGTATGTTGCCGCGGTCTTAAACACAAATGCGACGGTATCTCCGCGGTCTGTTTCTTTTATCCAACATCTCCCGCGACTCTGTAATCATAGCTACAGCCCCTTCCTa                                                                                                         <  8:1883/137‑1 (MQ=255)
                                        ccggcggGCATACCGAACGTGCAGCGGAAGTGGCCGCGGACTGAAACACAAACGCGACGGTCTCGCCGCGGGCTGTTGCTTTTACCCACCACCTGCCGCGACTCTGTAATCATAGCTACAGCCCCTTCCTACTCCCTGa                                                                                                 <  7:79995/138‑1 (MQ=255)
                                         gggggggAATACAGAACGTGCATCGTATGTGGCTGCGGTTTTTAACATAAATGCGACGGTCTCTCTGCGTTGTGTTTTTTTAAACAAAAATCTCCCGCGACTCTGTAATCATAGCTACAGCCCCTTCCTACTCCCTGa                                                                                                 <  8:167899/134‑1 (MQ=255)
                                         cgggggGCAAACAGTCCGTGCTTCGGACGTTGCTGGGGTCTGAAACATAAAGGCGACGGGCTCGCCGCGGGCTGTTTCTTTTACCCAACATGTCCCGCGACTCTGTAATCATAGCTACAGCCCCTTCCTACTCCCTGa                                                                                                 <  4:279854/138‑1 (MQ=255)
                                            cggGCATACAGTACGTGCATCGGAAGTTGCAGCGGTCTGTAACAGAAATGCGAAGGTCTCTCAGCGGTCTGTTTCTTTTATCCAACATCTGACGCGACTCTGTAATCATAGCTACAGCCCCTTCCTACTCCCTGATtct                                                                                             >  1:223957/1‑139 (MQ=255)
                                                    attttcGTGCATCGGATTTGGATGCGGTTTTTAACATAATTGCGATGGGCTCTCAGCGGTCTGTTTTTTTTAACAAACATCTCCCGCGACTCTGTAATCATAGCTACAGCCCCTTCCTACTCCCTGATTCTCTCAGGgt                                                                                     <  8:60924/134‑1 (MQ=255)
                                                         cGTGCATCGGAAGTTGCAGCGGTCTGTACCAGAAATGCGAAGGTCTCTCAGCGGTCTGTTTCTTTTATCCAACATCTGACGCGACTCTGTAATCATAGCTACAGCCCCTTCCTACTCCCTGATTCTCTCAGGGTGTGta                                                                                >  4:148346/1‑139 (MQ=255)
                                                         cGTGCATCGGAAGTTGCAGCGGTCTGTAACAGATATGCGAAGCTCTCTCAGCGGTCTGTTTCTTTTATCCAACATCTGAAGCGACTCTGTAATCATAGCTACGGCCCCTTCCTACTCCCAGATTCTCTGAGGGTGGGta                                                                                >  5:85188/1‑139 (MQ=255)
                                                         cGTGCATCGGAAGTTGCAGCGGTCTGTAACAGAAATGCGAAGGTCTCTCAGCGGTCTGTTTCTTTTATCCAACATCTGACGCGACTCTGTAATCATAGCTTCAGCCCCTTCCTACTCCCTGATTCTCTCAGGGTGTGta                                                                                >  3:253328/1‑139 (MQ=255)
                                                         cGTGCATCGGAAGTTGCAGCGGTCTGTAACAGAAATGCGAAGGTCTCTCAGCGGTCTGTTTCTTTTATCCAACATCTGACGCGACTCTGTAATCATAGCTACAGCCCCTTCCTACTCCCTGATTCTCTCAGGGTGTGta                                                                                >  2:225599/1‑139 (MQ=255)
                                                         cGTGCATCGGAAGTTGCAGCGGTCTGTAACAGAAATGCGAAGGTCTCTCAGCGGTCTGTTTCTTTTATCCAACATCTGACGCGACTCTGTAATCATAGCTACAGCCCCTTCCTACTCCCTGATTCTCTCAGGGTGTGta                                                                                >  6:50305/1‑139 (MQ=255)
                                                          gTGCATCGGAAGTTGCAGCGGTCTGTAACAGAAATGCGAAGGTCTCTCAGCGGTCTGTTTCTTTTATCCAACATCTGACGCGACTCTGTAATCATAGCTACAGCCCCTTCCTACTCCCTGATTCTCTCAGGGTGTGta                                                                                >  3:217829/1‑138 (MQ=255)
                                                           tGCATCGGAAGTTGCAGCGGTCTGTAACAGAAATGCGAAGGTCTCTCAGCGGTCTGTTTCTTTTATCCAACATCTGACGCGACTCTGTAATCATAGCTACAGCCCCTTCCTACTCCCTGATTCTCTCAGGGTGTGtata                                                                              >  3:125588/1‑139 (MQ=255)
                                                             cATCGGAAGTTGCAGCGGTCTGTAACAGAAATGCGAAGGTCTCTCAGCGGTCTGTTTCTTTTATCCAACATCTGACGCGACTCTGTAATCATAGCTACAGCCCCTTCCTACTCCCTGATTCTCTCAGGTTGTGtataga                                                                            >  5:95261/1‑137 (MQ=255)
                                                               tCGGAAGTTGCAGCGGTCTGTAACAGAAATGCGAAGGTCTCTCAGCGGTCTGTTTCTTTTATCCAACATCTGACGCGACTCTGTAATCATAGCTACAGCCCCTTCCTACTCCCTGATTCTCTCAGGGTGTGTATATAcc                                                                          >  1:88823/1‑138 (MQ=255)
                                                                 ggAAGTTGCAGCGGTCTGTAACAGAAATGCGAAGGTCTCTCAGCGGTCTGTTTCTTTTATCCTACATCTGCCGCGACTCTGTAATCATAGCTACAGCGCCTTCCTACTCCCTGATTCTCTCAGGGTGGGGATATACAt                                                                         >  3:271344/1‑138 (MQ=255)
                                                                 ggAAGTTGCAGCGGTCTGTAACAGAAATGCGAAGGTCTCTCAGCGGTCTGTTTCTTTTATCCAACATCTGCCGCGACTCTGTAATCATTGCTACAGCCCCTTCCTACTCCCTGAATCTCTCAGGGTGTGTAAATACag                                                                         >  6:195784/1‑137 (MQ=255)
                                                                 ggAAGTTGCAGCGGTCTGTAACAGAAATGCGAAGGTCTCTCAGCGGTCTGTTTCTTTTATCCAACATCTGACGCGACTCTGTAATCATAGCTACAGCCCCTTCCTACTCCCTGATTCTCTCAGGGTGTGTATATACAt                                                                         >  2:4236/1‑138 (MQ=255)
                                                                             ggTCTGTAACAGAAATGCGAAGGTCTCTCAGCGGTCTGTTTCTTTTATCCAACATCTGACGCGACTCTGTAATCATAGCTACAGCCCCTTCCTACTCCCTGATTCTCTCAGGGTGTGTATATACATTAAATGATACGtt                                                            >  6:243943/1‑139 (MQ=255)
                                                                              gTCTGTAACAGAAATGCGAAGGTCTCTCAGCGGTCTGTTTCTTTTATCCAACATCTGACGCGACTCTGTAATCATAGCTACAGCCCCTTCCTAATCCCTGATTCTCTCAGGTGTTGTATATAAATTAAATGTTGCGtt                                                            >  4:169288/1‑138 (MQ=255)
                                                                               tCTTTAACATAATTGCTATGGTCTCTCCGGGTTATTTTTATTTAAACAAACATCTTACGCGACTCTGTAATCATAGCTACAGCCCCTTCCTACTCCCTGATTCTCTCAGGGTGTGTATATACATTAAATGATCCGttt                                                           <  4:135024/138‑1 (MQ=255)
                                                                                                       ctcAGCGGTCTGTTTCTTTTATCCAACATCTGACGCGACTCTGTAATCATAGCTACAGCCCCTTCCTACTCCCTGa                                                                                                 <  7:730/76‑1 (MQ=255)
                                                                                                       ctcAGCGGTCTGTTTCTTTTATCCAACATCTGACGCGACTCTGTAATCATAGCTACAGCCCCTTCCTACTCCCTGa                                                                                                 >  8:730/1‑76 (MQ=255)
                                                                                                                        tttATCCAACATCTGACGCGACTCTGTAATCATAGCTACAGCCCCTTCCTACTCCCTGATTCTCTCAGGGTGTGTATATACATTAAATGATCCGTTTCTGACAAATCCAATCGCTGTGATATTCAATTCTTCAGCCAtt                 >  5:263622/1‑139 (MQ=255)
                                                                                                                         ttATCCAACATCTGACGCGACTCTGTAATCATAGCTACAGCCCCTTCCTACTCCCTGATTCTCTCAGGGTGTGTATATACATTAAATGATCCGTTTCTGACAAATCCAATCGCTGTGATATTCAATTCTTCAGCCAttt                >  3:201096/1‑139 (MQ=255)
                                                                                                                           agacAACCTCTGACGCGACTCTGTAATCATAGCTACAGCCCCTTCCTACTCCCTGCTTCTCTCAGGGTGTGTATATACATTAAATGATCCGTTTCTGACAAATCCAATCGCTGTGATATTCAATTCTTCCGCCATTTGa              <  4:271344/136‑1 (MQ=255)
                                                                                                                           aTCCAACATCTGACGCGACTCTGTAATCATAGCTACAGCCCCTTCCTACTCCCTGATTCTCTCAGGGTGTGTATATACATTAAATGATCCGTTTCTGACAAATCCAATCGCTGTGATATTCAATTCTTCAGCCATTTg               >  5:131580/1‑138 (MQ=255)
                                                                                                                                 cATCTGACGCGACTCTGTAATCATAGCTACAGCCCCTTCCTACTCCCTGATTCTCTCAGGGTGTGTATATACATTAAATGATCCGTTTCTGACAAATCCAATCGCTGTGATATTCAATTCTTCAGCCATTTGAATCGc         >  7:213773/1‑138 (MQ=255)
                                                                                                                                       aCGCGACTCTGTAATCATAGCTACAGCCCCTTCCTACTCCCTGATTCTCTCAGGGTGTGTATATACATTAAATGATCCGTTTCTGACAAATCCAATCGCTGTGATATTCAATTCTTCAGCCATTTGAATCGCAAGCTcc  >  7:274849/1‑139 (MQ=255)
                                                                                                                                       |                                                                                                                                          
CTCCTCTTTCTTTAAAAAAGGATAATCCGGGCCGAATAATTCGGCGGGCATACAGTACGTGCATCGGAAGTTGCAGCGGTCTGTAACAGAAATGCGAAGGTCTCTCAGCGGTCTGTTTCTTTTATCCAACATCTGACGCGACTCTGTAATCATAGCTACAGCCCCTTCCTACTCCCTGATTCTCTCAGGGTGTGTATATACATTAAATGATCCGTTTCTGACAAATCCAATCGCTGTGATATTCAATTCTTCAGCCATTTGAATCGCAAGCTCC  >  CP006881/3591053‑3591326

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑