Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP006881	3,723,121	T→A	100%	intergenic (‑235/‑66)	U712_19190 ← / → U712_19195	PTS system sucrose‑specific EIIBC component/putative transporter ywcJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	3,723,121	0	T	A	100.0%	223.3 / NA	65	intergenic (‑235/‑66)	U712_19190/U712_19195	PTS system sucrose‑specific EIIBC component/putative transporter ywcJ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (22/43);  total (22/43)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CTTTACCAGTCACAATCCCGCTTATTCAGATTGAGAATACGCTTTCATCATAAATTATGATAGCGTTTTCGTCAAC‑T‑GTTTTTTAGTTAAATAGTTTGATAAAACTTTTAGACAAAAATTCGTAAAAACTATTTGTGAAAAACTTCACAATATCGTGCTATACTATGCTCAACCATCAAAGAAAGCAGGAAAAAACAATGGAAACTCAAGCATTGCAAAAAGTTGAACAGTACGCTTTGAACAAACTAAACATATTTGTTT  >  CP006881/3722990‑3723250                                                                                                                                      |                                                                                                                                  cTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TAATTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATtgtg                                                                                                                              <  1:273692/138‑1 (MQ=255)    tACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaa                                                                                                                            <  4:765/137‑1 (MQ=255)      ccAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaata                                                                                                                         <  4:248979/138‑3 (MQ=255)      ccAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaata                                                                                                                         <  4:131934/138‑3 (MQ=255)      ccAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaata                                                                                                                         <  5:11592/138‑3 (MQ=255)      ccAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaata                                                                                                                         <  2:241752/138‑3 (MQ=255)          tCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTca                                                                                                                    >  1:101558/1‑139 (MQ=255)           caaaaTACCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTATTTAAATATTTTCATATAACATGAAGACAAAAATTCGTAAAAATTAATTGTGAAAAACTTca                                                                                                                    >  4:54630/4‑138 (MQ=255)               aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                                                                               <  1:51254/139‑1 (MQ=255)               aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                                                                               <  6:104952/139‑1 (MQ=255)               aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                                                                               <  3:16476/139‑1 (MQ=255)               aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                                                                               <  3:122549/139‑1 (MQ=255)               aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                                                                               <  3:111443/139‑1 (MQ=255)               aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                                                                               <  6:147130/139‑1 (MQ=255)               aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                                                                               <  2:256909/139‑1 (MQ=255)               aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                                                                               <  6:225821/139‑1 (MQ=255)               aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                                                                               <  2:170634/139‑1 (MQ=255)               aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTAGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                                                                               <  2:102353/139‑1 (MQ=255)               aTCCCGCGTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TAATTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                                                                               <  1:118431/139‑1 (MQ=255)                  ccGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTGGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATc                                                                                                             <  3:222881/138‑1 (MQ=255)                    gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACTAAATTTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                                                                                                          <  3:120212/139‑1 (MQ=255)                    gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCTTAAAAATTAATTGTGAAATACTTCACAATATCGTg                                                                                                          <  6:222120/139‑1 (MQ=255)                    gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                                                                               <  7:182967/134‑1 (MQ=255)                    gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                                                                               >  8:182967/1‑134 (MQ=255)                    gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                                                                                                          <  4:46757/139‑1 (MQ=255)                    gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                                                                                                          >  1:35223/1‑139 (MQ=255)                    gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                                                                                                          <  7:67661/139‑1 (MQ=255)                    gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                                                                                                          <  3:229083/139‑1 (MQ=255)                    gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                                                                                                          <  5:239507/139‑1 (MQ=255)                    gCTTATTCAGATTAAGAATACGCGTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                                                                                                          <  4:23412/139‑1 (MQ=255)                        aTTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTca                                                                                                                    <  6:92199/125‑1 (MQ=255)                        aTTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTca                                                                                                                    >  5:92199/1‑125 (MQ=255)                        aTTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCt                                                                                                        <  6:16745/137‑1 (MQ=255)                         ttCATATTTAGTATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAATTACTTCACAATATCGTGCtata                                                                                                     <  6:22333/139‑1 (MQ=255)                              aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                                                                               <  8:202578/124‑1 (MQ=255)                              aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                                                                               >  7:202578/1‑124 (MQ=255)                              aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATg                                                                                                >  8:216753/1‑139 (MQ=255)                              aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATg                                                                                                >  8:69556/1‑139 (MQ=255)                              aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATg                                                                                                >  1:125250/1‑139 (MQ=255)                                  agaATACGCTTTCATCATAAATCAAGATAGCGTTTTCGTCAACGA‑TTTTTTTAGTTAAATAGTTTGATTTAACATTTAGACATATATTAGTAAAAATTAATTGTGAAATACTTCACAATATCTTGCTATACTATGCtg                                                                                             >  3:184622/1‑137 (MQ=255)                                        cGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                                                                               >  1:103136/1‑114 (MQ=255)                                        cGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                                                                               <  2:103136/114‑1 (MQ=255)                                        cGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTTTACTATGCTCAATCAt                                                                                       >  4:15226/1‑138 (MQ=255)                                        cGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑T‑ATTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCAtg                                                                                      >  6:187543/1‑137 (MQ=255)                                        cGCTTTCATCATAAATCATGATAGCGTTTACGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAAATAGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCAt                                                                                       >  3:108542/1‑138 (MQ=255)                                              catcatAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATg                                                                                                <  3:241621/123‑1 (MQ=255)                                              catcatAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGAAAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATg                                                                                                >  4:241621/1‑123 (MQ=255)                                                tcatAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGaaagaaag                                                                              <  1:187173/139‑1 (MQ=255)                                                   tAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAg                                                                           >  1:90810/1‑139 (MQ=255)                                                      atcaTGATATAGTTTTCTTAAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATTTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATGGTGCTATACTATGCTAAATCATGAAAGAAAGCAGGaa                                                                        <  3:54630/136‑1 (MQ=255)                                                                     tCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACt                                                         >  4:3889/1‑139 (MQ=255)                                                                            actatttttttAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCa                                                   <  7:69556/134‑1 (MQ=255)                                                                                    tttAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGCCAATGGAAACTCAAGCATTACaaa                                            <  4:184622/138‑1 (MQ=255)                                                                                    tttAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACaaa                                            <  2:125250/138‑1 (MQ=255)                                                                                    tttAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACaaa                                            <  5:187543/138‑1 (MQ=255)                                                                                    tttAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACaaa                                            <  2:90810/138‑1 (MQ=255)                                                                                            aaTAGTTTGATATAACATGTAGTCAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAATCAGGAAAAGACTATGGAAACTCAAGCATTACAAAAGGTTGAAc                                   <  4:108542/139‑1 (MQ=255)                                                                                            aaTAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGaa                                    <  2:196364/138‑1 (MQ=255)                                                                                                           catgtAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGaaa                     >  2:214655/5‑137 (MQ=255)                                                                                                           catgtAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGaaa                     >  6:286364/5‑137 (MQ=255)                                                                                                           catgtAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGaaa                     >  2:116577/5‑137 (MQ=255)                                                                                                           catgtAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGaaa                     >  1:43628/5‑137 (MQ=255)                                                                                                               tAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCACGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAAc                 <  6:218429/138‑1 (MQ=255)                                                                                                                aGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAAca                >  5:99774/1‑137 (MQ=255)                                                                                                                              aaaaaTTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATAttcgctt  <  2:43628/138‑6 (MQ=255)                                                                                                                                      |                                                                                                                                  CTTTACCAGTCACAATCCCGCTTATTCAGATTGAGAATACGCTTTCATCATAAATTATGATAGCGTTTTCGTCAAC‑T‑GTTTTTTAGTTAAATAGTTTGATAAAACTTTTAGACAAAAATTCGTAAAAACTATTTGTGAAAAACTTCACAATATCGTGCTATACTATGCTCAACCATCAAAGAAAGCAGGAAAAAACAATGGAAACTCAAGCATTGCAAAAAGTTGAACAGTACGCTTTGAACAAACTAAACATATTTGTTT  >  CP006881/3722990‑3723250

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑