Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 353,697 T→G 21.4% F279V (TTT→GTT)  U712_01685 → Ferredoxin‑‑NADP reductase 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP006881353,6970TG21.4% 61.8 / 2.5 35F279V (TTT→GTT) U712_01685Ferredoxin‑‑NADP reductase 1
Reads supporting (aligned to +/- strand):  ref base T (11/16);  new base G (4/4);  total (15/20)
Fisher's exact test for biased strand distribution p-value = 7.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TAGAAATTGATGAGCTGATCATTAATCATGGGTTCAAAATTGATCTCGGGCCGATGATGGAGTGGGGGCTGGAGATTGAAGAAGGAAGGGTGAAAGCTGACAGACATATGCGAACGAATCTTCCGGGCGTGTTTGTGGCGGGCGACGCGGCTTTTTATGAAAGCAAGCTGAGATTGATTGCCGGCGGATTTACGGAAGGCCCGACAGCGGTCAACAGTGCCAAGGCTTATCTGGACCCTAAGGCCGAGAATATGGCGATGTATT  >  CP006881/353566‑353829
                                                                                                                                   |                                                                                                                                    
tAGAAATTGATGAGCTGATCATTAATCATGGGTTCAAAATTGATCTCGGGCCGATGATGGAGTGGGGGCTGGAGATTGAAGAAGGAAGGGTGAAAGCTGACAGACATCTGCGAACGAATCTTCCGGGCGTGTTTGTggc                                                                                                                               >  6:221022/1‑139 (MQ=255)
tAGAAATTGATGAGCTGATCATTAATCATGGGTTCAAAATTGATCTCGGGCCGATGATGGAGTGGGGGCTGGAGATTGAAGAAGGAAGGGTGAAAGCTGACAGACATATGCGAACGAATCTTCCGGGCGTGGTTGTggc                                                                                                                               >  7:273202/1‑139 (MQ=255)
   aaaTTGATGAGCTGATCATTAATCATGGGTTCAAAATTGATCTCGGGCCGATGATGGAGTGGGGGCTGGAGATTGAAGAAGG‑AGGGTGAAAGCTGACAGACATATGCGAACGAATCTTCCGGGCGTGTTTGTggcggg                                                                                                                            <  2:4699/138‑1 (MQ=255)
       tgatgaGCTGATCATTAATCATGGGTTCAAAATTGATCTCGGGCCGATGATGGAGTGGGGGCTGGAGATTGAAGAAGGAAGGGTGAAAGCTGACAGACATATGCGAACGAATCTTCCGGGCGTGTTTGTGGCGGGCGAc                                                                                                                        >  7:243139/1‑139 (MQ=255)
              cTGATCATTAATCATGGGTTCAAAATTGATCTCGGGCCGATGATGGAGTGGGGGCTGGAGATTGAAGAAGGAAGGGTGAAAGCTGACAGACATATGCGAACGAATCTTCCGGGATTGTTTTTGGCGGGGGACGCggggt                                                                                                                 >  2:273170/1‑136 (MQ=255)
                        atgaTGGGTTCAAATTTGTTCTCGGTCCGTTGATGGAGTGGGGGATGGAGATTGAAGAAGGAAGGGTGAAAGCTGACAGACATATGCGAACGAATCTTCCGGGCGTGTTTGTGGCGGGCGACGCGGCTTTTTATGAaag                                                                                                       <  2:96476/136‑1 (MQ=255)
                           aTGGGTTCAAAATTGATCTCGGGCCGATGATGGAGTGGGGGCTGGAGATTGAAGAAGGAAGGGTGAAAGCTGACAGACATATGCGAACGAATCTTCCGGGCGTGTTTGTGGCGGGCGAtgc                                                                                                                      >  7:218083/1‑121 (MQ=255)
                           aTGGGTTCAAAATTGATCTCGGGCCGATGATGGAGTGGGGGCTGGAGATTGAAGAAGGAAGGGTGAAAGCTGACAGACATATGCGAACGAATCTTCCGGGCGTGTTTGTGGCGGGCGAggc                                                                                                                      <  8:218083/121‑1 (MQ=255)
                             gtgTTCAAAATTTTTCTCGGGCCGATGATGGAGTGGGGGCTGGAGATTGAAGAAGGAAGGGTGAAAGCTGACAGACATATGCGAACGAATCTTCCGGGCGTGTTTGTGGCGGGCGACGCGGCTTTTTATGAaagcaag                                                                                                   <  1:273170/136‑1 (MQ=255)
                              ggTTCAAAATTGATCTCGGGCCGATGATGGAGTGGGGGCTGGAGATTGAAGCAGGAAGGGTGAAAGCTGACAGACATATGCGAACGAATCTTCCGGGCGTGTTTGTGGCGGGCGACGCGGCTTTTTATGAAAGCAAGCt                                                                                                 <  6:99394/139‑1 (MQ=255)
                                          aTCTCGGGCCGATGATGGAGTGGGGGCTGGAGATTGAAGAAGGAAGGGTGAAAGCTGACAGACATATGCGAACGAATCTTCCGGGCGTGGTTGTGGCGGGCGACGCGGCTTTTTATGAAAGCAAGCTGAGATTGATTGc                                                                                     >  7:254568/1‑139 (MQ=255)
                                                               gggggCTGGAGATTGAAGAAGGAAGGGTGAAAGCTGACAGACATATGCGAACGAATCTTCCGGGCGTGTTTGTGGCGGGCGACGCGGCTTTTTATGAAAGCAAGCTGAGattgattg                                                                                      >  8:84451/1‑117 (MQ=255)
                                                               gggggCTGGAGATTGAAGAAGGAAGGGTGAAAGCTGACAGACATATGCGAACGAATCTTCCGGGCGTGTTTGTGGCGGGCGACGCGGCTTTTTATGAAAGCAAGCTGAGattgattg                                                                                      <  7:84451/117‑1 (MQ=255)
                                                                 gggCTGGAGATTGAAGCAGGAAGGGTGAAAGCTGACAGACATATGCGCACGAATCTTCCGGGCGTGGTTGTGGCGGGCGACGCGGCTTTTTATGAAAGCAAGCTGAGATTGATTGCCGGCGGATTTACGGAAGGCCCGa                                                              <  5:52412/139‑1 (MQ=255)
                                                                   gCTGGAGATTGAAGAAGGAAGGGTGAAAGCTGACAGACATATGCGAACGAATCTTCCGGGCGTGTTTGTGGCGGGCGACGCGGCTTTTTATGAAAGCAAGCTGAGATTGATTGCCGGCGGATTTACGGAAGGCCCGACa                                                            <  3:115267/139‑1 (MQ=255)
                                                                            tGAAGAAGGAAGGGTGAAAGCTGACAGACATATGCGAACGAATCTTCCGGGCGTGTTTGTGGCGGGCGACGCGGCTTTTTATGAAAGCAAGCTGAGATTGATTGCCGGCGGATTTACGGAAGGCCCGACAGCGGTCaa                                                    >  5:28599/1‑138 (MQ=255)
                                                                            tGAAGAAGGAAGGGTGAAAGCTGACAGACATATGCGAACGAATCTTCCGGGCGTGGTTGTGGGGGGCGACGCGGCGTTTTATGGAAGCAAACTGAGCTTGCTTGCCGGCGGATTTACGGTAGGCCCGCCCGCgggcca                                                    >  3:166991/1‑134 (MQ=255)
                                                                                   ggaaggGTGAAAGCTGACAGACATATGCGAACGAATCTTCCGGGCGTGTTTGTGGCGGGCGACGCGGCTTTTTATGAAAGCAAGCTGAGATTGATTGCCGGCGGATTTACGGAAGGCCCGACAGCGGTCAAc                                                   <  8:174620/132‑1 (MQ=255)
                                                                                   ggaaggGTGAAAGCTGACAGACATATGCGAACGAATCTTCCGGGCGTGTTTGTGGCGGGCGACGCGGCTTTTTATGAAAGCAAGCTGAGATTGATTGCCGGCGGATTTACGGAAGGCCCGACAGCGGTCAAc                                                   >  7:174620/1‑132 (MQ=255)
                                                                                      aggGTGAAAGCTGACAGACATATGCGAACGAATCTTCCGGGCGTGGTTGTGGCGGGCGACGCGGCTTTTTATGAAAGCAAGCTGAGATTGATTGCCGGCGGATTTACGGAAGGCCCGACAGCGGTCAACAGTGCCAAgg                                         <  8:254568/139‑1 (MQ=255)
                                                                                        ggTGAAAGCTGACAGACATATGCGAACGAATCTTCCGGGCGTGTTTGTTGCGGGCGACGCGGCGTTTTATTAAAGCAAGCTGAGATTGATTGCCGGCGGATTTTCGGAAGGCCCGAACGCGGTCAACAGGGCCAAGGCt                                       >  4:283581/1‑139 (MQ=255)
                                                                                        ggTGAAAGCTGACAGACATATGCGAACGAATCTTCCGGGCGTGTTTGTGGCGGGCGACGCGGCTTTTTATGAAAGCAAGCTGAGATTGATTGCcgg                                                                                  <  4:45037/96‑1 (MQ=255)
                                                                                        ggTGAAAGCTGACAGACATATGCGAACGAATCTTCCGGGCGTGTTTGTGGCGGGCGACGCGGCTTTTTATGAAAGCAAGCTGAGATTGATTGCcgg                                                                                  >  3:45037/1‑96 (MQ=255)
                                                                                            aaaGCTGACAGACATATGCGAACGAATCTTCCGGGCGTGGTTGTGGGGGGCGCCCCGGCGTTTTTTGAAAGCAATCTGAGATTGATTGCAGGCGGATTTACGGAAGGCCCGCCAGCGGTCAACAGTGCCAAGGCGTAt                                    >  5:234759/1‑138 (MQ=255)
                                                                                              aGCTGACAGACATATGCGAACGAATCTTCCGGGCGTGTTTGTGGCGGGCGACGCGGCTTTTTATGAAAGCAAGCTGAGATTGATTGCCGGCGGATTTACGGAAGGCCCGACAGCGGTCAACAGTGCCAAGGCTTATCTg                                 <  3:124679/139‑1 (MQ=255)
                                                                                                          tatGCGAACGAATCTTCCGGGCGTGTTTGTGGCGGGCGACGCGGCTTTTTATGAAAGCAAGCTGAGATTGATTGCCGGCGGATTTACGGAAGGCCCGACAGCGGTCAACAGTGCCAAGGCTTATCTGGACCCTAAGGcc                     <  6:234759/139‑1 (MQ=255)
                                                                                                          tatGCGAACGAATCTTCCGGGCGTGTTTGTGGCGGGCGACGCGGCTTTTTATGAAAGCAAGCTGAGATTGATTGCCGGCGGATTTACGGAAGGCCCGACAGCGGTCAACAGTGCCAAGGATTATCTGGACCCTAAGGcc                     <  4:260032/139‑1 (MQ=255)
                                                                                                                 acgaaTCTTCCGGGCGTGTTTGTGGCGGGCGCCGCGGCTTTTTATGAAAGCAAGCTGAGATTGATTGCCGGCGGATTTACGGAAGGCCCGACAGCGGTCAACAGTGCCAAGGCTTATCTGGACCCTAAGGCCGAGAata              >  8:145663/1‑139 (MQ=255)
                                                                                                                   gaaTCTTCCGGGCGTGTTTGTGGCGGGCGACGCGGGGTTTTATGAAAACAAGCGGAGAATGATTGCCGGGGGATTTACGGAAGGCCCGCCAGCGGTCAACAGTGCCAAGGCTTATCAGGCCCCTAAAGCCGAGAATATg            >  2:161541/1‑139 (MQ=255)
                                                                                                                   gaaTCTTCCGGGCGTGTTTGTGGCGGGCGACGCGGCTTTTTATGAAAGCAAGCTGAGATTGATTGCCGGCGGATTTACGGAAGGCCCGACAGCGGTCAACAGTGCCAAGGCTTATCTGGACCCTAAGGCCGAGAATATg            <  6:28599/139‑1 (MQ=255)
                                                                                                                   gaaTCTTCCGGGCGTGGTTGGGGCGGGCGACGCGGCTTTTTATGAAAGCAAGCTGAGATTGATTGCCGGCGGATTTACGGAAGGCCCGACAGCGGTCAACAGTGCCAAGGCTTATCTGGACCCTAAGGCCGAGAATATg            <  4:166991/139‑1 (MQ=255)
                                                                                                                          ccGGGCGTGTTTGTGGCGGGCGACGCGGCTTTTTATGAAAGCAAGCTGAGATTGATTGCCGGCGGATTTACGGAAGGCCCGACAGCGGTCAACAGTGCCAAGGCTTATCTGGACCCTAAGGCCGAGAATATGGCg         <  1:161541/135‑1 (MQ=255)
                                                                                                                          ccGGGCGTGTTTGTGGCGGGCGACGCGGCTTTTTATGAAAGCAAGCTGAGATTGATTGCCGGCGGATTTACGGAAGGCCCGACAGCGGTCAACAGTGCCAAGGCTTATCTGGACCCTAAGGCCGAGAATATGGCg         <  8:125451/135‑1 (MQ=255)
                                                                                                                          ccGGGCGTGTTTGTGGCGGGCGACGCGGCTTTTTATGAAAGCAAGCTGAGATTGATTGCCGGCGGATTTACGGAAGGCCCGACAGCGGTCAACAGTGCCAAGGCTTATCTGGACCCTAAGGCCGAGAATATGGCg         <  3:19201/135‑1 (MQ=255)
                                                                                                                             ggCGTGGTTGTGGCGGGCGACGCGGCTTTTTATGAAAGCAAGCTGAGATTGATTGCCGGCGGATTTACGGAAGGCCCGACAGCGGTCAACAGTGCCAAGGCTTATCTGGACCCTAAGGCCGAGAATATGGCGATGTAtt  <  8:214098/139‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                    
TAGAAATTGATGAGCTGATCATTAATCATGGGTTCAAAATTGATCTCGGGCCGATGATGGAGTGGGGGCTGGAGATTGAAGAAGGAAGGGTGAAAGCTGACAGACATATGCGAACGAATCTTCCGGGCGTGTTTGTGGCGGGCGACGCGGCTTTTTATGAAAGCAAGCTGAGATTGATTGCCGGCGGATTTACGGAAGGCCCGACAGCGGTCAACAGTGCCAAGGCTTATCTGGACCCTAAGGCCGAGAATATGGCGATGTATT  >  CP006881/353566‑353829

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑