Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP006881	3,723,294	G→A	100%	M36I (ATG→ATA)	U712_19195 →	putative transporter ywcJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	3,723,294	0	G	A	100.0%	154.8 / NA	45	M36I (ATG→ATA)	U712_19195	putative transporter ywcJ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/21);  total (24/21)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AACCATCAAAGAAAGCAGGAAAAAACAATGGAAACTCAAGCATTGCAAAAAGTTGAACAGTACGCTTTGAACAAACTAAACATATTTGTTTCTAGCAAAATCCGTTATGTGCTCAGATCCATTTTGGCCAGCATGTTTATCGGATTCGGCATTACAGCCGCAAGCAAAACAGGCAGTTATTTCTCTATGGCGGATTCTCCGTTTGCTTTTCCGGCAGCCGCTGTCACATTCGGGGCCGCTATTCTGCTGATTGCTTACGGAGGCGG  >  CP006881/3723160‑3723425                                                                                                                                       |                                                                                                                                    aatcATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTa                                                                                                                                 >  1:179513/4‑139 (MQ=255) aatcATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTa                                                                                                                                 >  4:56256/4‑139 (MQ=255) aatcATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTa                                                                                                                                 >  2:112296/4‑139 (MQ=255) aatcATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCAGTTTGGCCAGTATATTTa                                                                                                                                 >  3:277988/4‑139 (MQ=255)      tgaaagaaagCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTggt                                                                                                                            >  8:24285/3‑138 (MQ=255)        aaagaaatCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAATATGATTTGAAAAAACAAAACATATTCGATTCAAGAAAAATCCGTTATGTGCTTCGGTCCATTTTGGACAGTATATTTATTggtat                                                                                                                          >  6:43058/1‑136 (MQ=255)        aaagaaagCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTggtt                                                                                                                           <  3:42427/138‑3 (MQ=255)        aaagaaagCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGttt                                                                                                                          <  3:45897/139‑1 (MQ=255)         aagaaagCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGtttt                                                                                                                         <  7:24285/139‑2 (MQ=255)         aagaaagCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGtttt                                                                                                                         <  7:4560/139‑2 (MQ=255)           gaaagCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTgg                                                                                                                       <  8:193712/139‑1 (MQ=255)                                   cTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAgg                                                                                               <  7:216753/139‑1 (MQ=255)                                          aTTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTAt                                                                                        <  1:87153/139‑1 (MQ=255)                                          aTTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTAt                                                                                        <  5:286364/139‑1 (MQ=255)                                          aTTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTAt                                                                                        <  8:243546/139‑1 (MQ=255)                                          aTTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTAt                                                                                        <  3:56256/139‑1 (MQ=255)                                              cAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTtct                                                                                    >  6:143424/1‑139 (MQ=255)                                                     ttGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGc                                                                                              <  4:144793/122‑1 (MQ=255)                                                     ttGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGc                                                                                              >  3:144793/1‑122 (MQ=255)                                                      tGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGct                                                                            >  7:115844/1‑138 (MQ=255)                                                        aaCAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGa                                                                          >  8:187483/1‑139 (MQ=255)                                                        aaCAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGa                                                                          >  1:85324/1‑139 (MQ=255)                                                          cAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGAtt                                                                        <  1:214655/139‑1 (MQ=255)                                                             tatgCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATtctc                                                                     >  1:183321/4‑139 (MQ=255)                                                                  tttGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGttt                                                                >  7:60646/1‑139 (MQ=255)                                                                  tttGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGttt                                                                >  4:36846/1‑139 (MQ=255)                                                                    tGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGc                                                                             <  3:196332/124‑1 (MQ=255)                                                                    tGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGc                                                                             >  4:196332/1‑124 (MQ=255)                                                                    tGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGc                                                              >  8:284657/1‑139 (MQ=255)                                                                       aaaaacaaaacaTATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCctt                                                           >  6:38543/3‑139 (MQ=255)                                                                             aaaaCATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGc                                                     <  5:122036/138‑1 (MQ=255)                                                                                            caaGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACttt                                      >  3:282372/3‑139 (MQ=255)                                                                                              aGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCg                                    >  5:192562/1‑139 (MQ=255)                                                                                               gCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCgg                                   <  2:182190/139‑1 (MQ=255)                                                                                                    aTCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCg                              <  2:101558/139‑1 (MQ=255)                                                                                                    aTCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCg                              <  4:282372/139‑1 (MQ=255)                                                                                                         ttATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCg                              <  1:266120/134‑1 (MQ=255)                                                                                                         ttATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCg                              >  2:266120/1‑134 (MQ=255)                                                                                                           aTGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTct                       >  5:153840/1‑139 (MQ=255)                                                                                                                cttcggtCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTctgatg                   <  4:280205/132‑1 (MQ=255)                                                                                                                cttcggtCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCtgatga                  <  8:60646/133‑1 (MQ=255)                                                                                                                        cATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTAc          >  1:166532/1‑139 (MQ=255)                                                                                                                        cATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCGATTCTGATGATTGCTTAc          >  1:205303/1‑139 (MQ=255)                                                                                                                               gCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCg   <  2:183321/139‑1 (MQ=255)                                                                                                                                ccAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCgg  >  6:44361/1‑139 (MQ=255)                                                                                                                                       |                                                                                                                                    AACCATCAAAGAAAGCAGGAAAAAACAATGGAAACTCAAGCATTGCAAAAAGTTGAACAGTACGCTTTGAACAAACTAAACATATTTGTTTCTAGCAAAATCCGTTATGTGCTCAGATCCATTTTGGCCAGCATGTTTATCGGATTCGGCATTACAGCCGCAAGCAAAACAGGCAGTTATTTCTCTATGGCGGATTCTCCGTTTGCTTTTCCGGCAGCCGCTGTCACATTCGGGGCCGCTATTCTGCTGATTGCTTACGGAGGCGG  >  CP006881/3723160‑3723425

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑