| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP006881 | 3,725,239 | 2 bp→AA | 100% | intergenic (‑71/‑457) | U712_19205 ← / → U712_19210 | Uncharacterized protein ywcI/Minor extracellular protease vpr |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 3,725,239 | 0 | G | A | 99.7% | 133.4 / ‑7.1 | 41 | intergenic (‑71/‑458) | U712_19205/U712_19210 | Uncharacterized protein ywcI/Minor extracellular protease vpr |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (23/17); minor base C (1/0); total (24/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| * | CP006881 | 3,725,240 | 0 | G | A | 100.0% | 107.2 / ‑6.6 | 41 | intergenic (‑72/‑457) | U712_19205/U712_19210 | Uncharacterized protein ywcI/Minor extracellular protease vpr |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (24/15); minor base C (0/1); total (24/17) | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAG‑C‑C‑T‑TTTAGCAGCCCGTTTT‑CTCAGCCGCAGCCCACGGGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAACCAA > CP006881/3725119‑3725352 || ttCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAG‑C‑CTT‑TTTAGCAGCCCGTTTT‑CTCAGCCGCAGCCCGCAAGAAAAGACGGCCGAtt > 6:290660/1‑139 (MQ=255)ttCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAG‑C‑CGT‑TTTAGCACCCCGTTTT‑CTCAGCCGCAGCCCGCAAGAAAAGACGGCCGAtt > 5:248937/1‑139 (MQ=255)ttCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCGGGTTTTTGGCAGGCCG‑C‑CTT‑TTTAGCAGCCCGTTTT‑CTAAGCCGCAGCCCGCCAGAAAACCCGGCCGCtt > 5:38436/1‑139 (MQ=255) catcaAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAG‑C‑CTT‑TTTAGCAGCCCGTTTT‑CTCAGCCGCAGCCCGCAAGAAAAGACGGCCGAtttc > 3:108914/1‑139 (MQ=255) caAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAG‑C‑CGT‑TTTAGCAGCCCGTTTT‑CTCAGCCGCAGCCCCCAAGAAAAGACGGCCGAtttcttt > 7:216765/1‑139 (MQ=255) accaccCACACCCGTAAAGATACCACCAACCTTTTCATACAAAAACACCCCTCCGCTTCTTTTTGGCCGGCAG‑C‑CTT‑TTTCGCCGCCCGTTTT‑CTCCGCCGCACCCCCCAACAAACGACGGCCGATTTCTTTTCTCGCa < 8:126348/139‑1 (MQ=255) aTCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCGTCTTTTTGGCAGGCAG‑C‑CTT‑TTTAGCAGCCCGTTTT‑CTCAGCCGCAGCCCGCAAGAAAAGCCGGCCGCTTTCTTTTCTCggcaac > 4:49135/1‑134 (MQ=255) cacaCCCGAAAAGATACACGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAG‑C‑CTT‑TTTAGCACCCCGTTTT‑CTCAGCCGCAGCCCGCAACAAAAGACGGCCGATTTCTTTTCTCGCAAACTAg < 4:87601/139‑1 (MQ=255) tAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAG‑C‑CTT‑TTTAGCAGCCCGTTTT‑CTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGctc > 5:209771/1‑137 (MQ=255) tAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAG‑C‑CTT‑TTTAGCAGCCCGTTTT‑CTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCGTTTGTCGCAAACTAGCGGcgc > 4:174587/1‑135 (MQ=255) aGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCCGGCAG‑C‑CTT‑TTTAGCCGCCCGTTTG‑CCCAGCCCCAGCCCGCAATAAACGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCa < 3:267661/139‑1 (MQ=255) gATACAAGCAAAGTTTTAATATAAAAACTTCCCTCCGCTTGTTTTTGCCTGGCAG‑CTT‑T‑TTTAGCAGCCGTTTTT‑CTCCGCCCCAGCCCGCAATAAAACACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCaa < 8:216765/139‑1 (MQ=255) aTACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAG‑C‑CTT‑TTTAGCCGCCCGTTTT‑CTCAGCCGCAGCCCGCAAGAAAAGCCGGCCGATATCTTTTCTCGCAAACTAGCGGCTCTAGCaa > 7:59037/1‑138 (MQ=255) aCAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAG‑C‑CTT‑TTTAGCAGCCCGTTTT‑CTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCaaaa > 1:153725/1‑138 (MQ=255) aCAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAG‑C‑CTT‑TTTAGCAGCCCGTTTT‑CTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCa > 8:209964/1‑135 (MQ=255) aCAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAG‑C‑CTT‑TTTAGCAGCCCGTTTT‑CTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCGTTTCTCGCAAACTAGCGGCTCGAGCaaaa > 2:206170/1‑138 (MQ=255) aCAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAG‑C‑CTT‑TTTAGCAGCCCGTTTT‑CTCAGCCGCAGCCCGCAAGAAAAGACGGCCGAGTTCTTTTCTCGCCAACTAGCGGCTCTATCAAAAc > 3:80157/1‑139 (MQ=255) aCAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAG‑C‑CTT‑TTTAGCAGCCCGTTTT‑CTCAGCCGCAGCCCGCAACAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCa < 7:209964/135‑1 (MQ=255) aaGCAAACGTTTAATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAG‑C‑CTT‑TTTAGCAGCCCGTTTT‑CTCAGACGCAGCCAGCAAGAAAAGACGGCCGATTTCTTTTCTGGCAAACTAGCGGCTCGAGCaaaaat > 7:239397/1‑136 (MQ=255) aGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAC‑C‑C‑TGTTTAGCAGCCCGTTTT‑CTCACCCCCAGCCCGCAAGAAAAGACGGCCGATATCTATTCTCGCAAACTATCGGCTCAAGCAAAACTCa > 8:98261/1‑139 (MQ=255) gCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAG‑C‑CTT‑TTTAGCAGCCCGTTTT‑CTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTATAGCAAACAACCGGCTCTAGCAAAACTCAt > 2:24214/1‑139 (MQ=255) gCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAG‑C‑CTT‑TTTAGCAGCCCGTTTT‑CTAAGCCGCAGCCCGCAAGAAAAGACGGCCCAGTTCGTTTATAGCAAACTAGGCGCTGGAGCAAAACGCAt > 1:123458/1‑139 (MQ=255) ccAAACGTTACATACAAAAACATCCCTCCGCTGATTTTTGGCAGCCAG‑C‑CTT‑TTTAGCAGCCCTTTTT‑CTCAGCCCCAGCCCCCAATAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCAt < 5:141936/138‑1 (MQ=255) aaccGTTCCATATAAAAAAATCCCCCCGCTTTTTTTTGGATGCCAC‑C‑CTT‑TTTAGACGCCCTTTTA‑AACCGCCGCCCCCCGCACTAAAACACGGCCGATTTCTTTTCTCGAAACCTAGCGGCTCTAGCAAAACTCATGa < 8:239397/136‑1 (MQ=255) aaaCGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAG‑C‑CTT‑TTTAGCAGCCCGTTTT‑CTCCGCCGCCGCCCGCAAGAAACGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGa < 5:140468/139‑1 (MQ=255) ccGTTTCATCCAAAAACATCCCGCCGCTTCTTTTTGGCTGGCAG‑C‑CTT‑TTTAGCCCCCCGTTTT‑CTCCGCCGCACCCCCCATTAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGAct < 6:280972/138‑1 (MQ=255) acttttCAAATAAAAAAATCCCTCCGCTTATTTTTGGCCGCCAG‑C‑CTT‑TTTAGCCGCCCGTTTT‑CTCAGCCGCCGCCCGAAATAAACGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGAct < 8:160178/136‑1 (MQ=255) acttttAATAGAAAAACATCCCTCCGCTGCTTTTTGGCAGGCTG‑C‑CTT‑TTTCCCAGCCGGTTTT‑CACCGCCGCCGCCCGCAAGAACCGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGAct < 3:141423/136‑1 (MQ=255) aCGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAG‑C‑CTT‑TTTAGCAGCCCGTTTT‑CTCAGCCGCAGCCCGCAAGAAAAGACAGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGAct > 3:275209/1‑139 (MQ=255) aCGTTTAATATAAAAACATCCCTCCGATTATTTTTGGCAGGATGAC‑T‑T‑TTTAGCAGCCCTTTTG‑CTCAGCCGCACCCCGCAAAAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGAct < 7:98261/139‑1 (MQ=255) tCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAG‑C‑CTT‑TTTAGCAGCCCGTTTT‑CTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTAt > 7:220237/1‑138 (MQ=255) tCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAG‑C‑CTT‑TTTAGCAGCCCGTTTT‑CTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCCGCTCTAGCAAAACTCAAGACTCTAt > 1:23684/1‑138 (MQ=255) cATAGAAAACCAACCCTCCGCTTCTTTTTGCCAGCCAG‑C‑CTT‑TTTAGCAGCCCGTTTT‑CTCAGCCGCAGCCCCCAACAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGa < 2:9319/139‑1 (MQ=255) aaaaaCATCCCTCCGCTTCTTTTTGGCAGGCAG‑C‑CTT‑TTTAGCAGCCCGTTTT‑CTCAGCCGCCGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACAAGCGGCTCTAGCAAAACTCAGTACTCgatgatgtgt > 7:251909/1‑135 (MQ=255) aaaaaCACCCCTCCTCTTCTTTTGGGCCGGCCC‑C‑A‑T‑TTTAACCCCCCCTTTTCCTCCCCCCCCCCCCCCAAAAAACCCCGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGAc < 4:108914/139‑1 (MQ=255) cccTCCGCTTTTTTTTGGCCGGCCG‑CTT‑T‑TTTCGCAGCCGTTTTT‑CACCCCCGCCCCCCCAAACAACACACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGaaa < 2:23684/139‑1 (MQ=255) cccTCCGCTTCTTTTTGGCTGGCAG‑C‑CTT‑TTTAGCAGCCGGTTTT‑CTCCGCCGCAGCCCGAAAGAAAACACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGaaa < 8:220237/139‑1 (MQ=255) gcag‑C‑CTT‑TTTAGCAGCCCGTTTT‑CTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCAt > 8:290324/1‑95 (MQ=255) gcag‑C‑CTT‑TTTAGCAGCCCGTTTT‑CTCAGCCGCAGCCCGAAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCAt < 7:290324/95‑1 (MQ=255) agcCCGTTTT‑CTCAGCCGCAGCCCGCAAGAAAAGCCGGCCGATTTATTTTCTCGCAAACTAGCGGCTCTAGCAAAACGCATGACGCTATGATAGACCGTGGAAAGAGAATTCGTTGTAACAATATGACTTaaaaaaac > 1:255426/1‑136 (MQ=255) cccGTTTT‑CTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAACCaa > 1:104886/1‑138 (MQ=255) || TTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAG‑C‑C‑T‑TTTAGCAGCCCGTTTT‑CTCAGCCGCAGCCCACGGGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAACCAA > CP006881/3725119‑3725352 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |