| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | CP006881 | 2,963,948 | G→A | intergenic (‑135/+145) | U712_15285 ← / ← U712_15290 | Manganese‑binding lipoprotein mntA/o‑succinylbenzoate synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 2,963,948 | 0 | G | A | 96.3% | 174.1 / NA | 54 | intergenic (‑135/+145) | U712_15285/U712_15290 | Manganese‑binding lipoprotein mntA/o‑succinylbenzoate synthase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (45/7); minor base C (0/2); total (45/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.52e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TATTTCTCCTCCTCTTTGCCATCAATGTCCGATTGTTTTATCTTATGTTGTCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCGCGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACGAACACTTTTACTGTAACAGCACAGTGTCATTTATTCAAAATAGCAAA > CP006881/2963813‑2964081 | tatTTCTCCTCCTCTTTGCCATCAATGTCCGATTGTTTTATCTTATGTTGTCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCACGt > 7:254156/1‑139 (MQ=255) ctTTGCCATCAATGTCCGATTGTTTTATCTTATTTTGTCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATATACACAGGACAAAAAGATTCCCTCATGCAAAATTATCACTTTTGGCCATTTTTg > 7:358976/1‑139 (MQ=255) ctTTGCCATCAATGTCCGATTGTTTTATCTTATGTTGTCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCACGTTTGGCCATTTTTg > 5:149017/1‑139 (MQ=255) tGCCATCAATGTCCGATTGTTTTATCTTATGTTGTCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCACGTTTGGCCATTTTTGTc > 5:313458/1‑138 (MQ=255) tGCCATCAATGTCCGATTGTTTTATCTTATGTTGTCAGTTCTTAGACATGTGACATTAAATAAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCGCTCATGCAAAATTATCACGTTTGGCCATTTTTGTc > 8:107973/1‑138 (MQ=255) ccATCAATGTCCGATTGTTTTATCTTATGTTGTCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAg < 1:109603/139‑1 (MQ=255) cAATGTCCGATTGTTTTATCTTATGTTGTCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTGa > 3:125526/1‑139 (MQ=255) aaTGTCCGATTGTTTTATCTTATGTTGTCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTGAt > 3:277421/1‑139 (MQ=255) aaTGTCCGATTGTTTTATCTTATGTTGTCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTGAt > 5:32056/1‑139 (MQ=255) aaTGTCCGATTGTTTTATCTTATGTTGTCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTGAt < 2:289274/139‑1 (MQ=255) tCCGATTGTTTTATCTTATGTTGTCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTGATTTa > 8:159900/1‑138 (MQ=255) tgttgtCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGATTCCCTCATGTAAAATTATCACGTTTGGCCATTTTTGTCAAGATGATTTACATAAAAAAACCGGCTggg > 7:249673/1‑139 (MQ=255) tCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTcagc > 5:352012/1‑139 (MQ=255) tCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTcag > 3:232481/1‑138 (MQ=255) tCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTcag > 2:204982/1‑138 (MQ=255) tCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTcag > 1:366388/1‑138 (MQ=255) tCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTcag > 1:193317/1‑138 (MQ=255) ttCTTAGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGcc > 2:38628/1‑139 (MQ=255) aGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCt < 7:229252/122‑1 (MQ=255) aGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCt > 8:229252/1‑122 (MQ=255) aGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTc > 1:48272/1‑138 (MQ=255) tgtgACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAg < 7:262534/93‑1 (MQ=255) tgtgACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAg > 8:262534/1‑93 (MQ=255) tgtgACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCTGCTGGGTCAGCAGCCGTTCCAAAc > 8:22406/1‑138 (MQ=255) tgtgACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAAc > 3:142899/1‑138 (MQ=255) tgtgACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAAc > 2:151442/1‑138 (MQ=255) tgtgACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAAc > 5:150346/1‑138 (MQ=255) tgtgACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAAc > 8:227003/1‑138 (MQ=255) tgtgACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAAc > 3:11532/1‑138 (MQ=255) gtgACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAAc > 7:150825/1‑137 (MQ=255) aTTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATg > 3:155653/1‑138 (MQ=255) gCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACTAGAAGGGATGTAtt > 3:38941/1‑138 (MQ=255) gCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTAtt > 1:241197/1‑138 (MQ=255) gCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTAta > 5:123177/1‑137 (MQ=255) aaGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACg > 2:331534/1‑138 (MQ=255) aaGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACGa > 2:260287/1‑139 (MQ=255) tGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACGAACACt > 5:276899/1‑139 (MQ=255) tGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACGAACACt > 6:171240/1‑139 (MQ=255) tGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACGAACACt > 3:52388/1‑139 (MQ=255) tGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTCATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACTAACACt > 4:136244/1‑139 (MQ=255) atttaAAAAGCAAAAAATTTTTCCCTCTTCAAAAATTATCACGTTTGCCTATTTTTTACAAGGTTATTTAAAAAAAAAAACCGGGTGGGTCAGCAGCGGGTCCAAACGAGAAGGGATGTATTCAACGAACACTTTTAc < 2:48272/138‑1 (MQ=255) attaaCACAGCAAAAACATTTCCCCAAATCAAAAATTAACCTGTTTGGCATTTTTTTAAAAGGTTATTAAAAAAAAAAACCCGGCTGGGTCCGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACGAACACTTTTAc < 6:352012/138‑1 (MQ=255) caggcaCAAAAATTTTCCATCATTAAAAATTATCCTGTTTGGCATTTTTTTTCAAGATTATTAAAATAAAAAACCCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACGAACACTTTTACTGTAACa < 2:107466/134‑1 (MQ=255) acaGGACAAAAAGTTTCCCTCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCt > 2:61187/1‑80 (MQ=255) acaGGACAAAAAGTTTCCCTCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCt < 1:61187/80‑1 (MQ=255) gACAAAAAGTTTCCCTCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACGAACACTTTTACTGTAACAGcac > 6:102935/1‑139 (MQ=255) gACAAAAAGTTTCCCTCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACGAACACTTTTACTGTAACAGca > 1:324264/1‑138 (MQ=255) gACAAAAAGTTTCCCTCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACGAACACTTTTACTGTAACAGca > 4:161171/1‑138 (MQ=255) ttCCCTCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACGAACACTTTTACTGTAACAGCACAgtgctgtct > 5:160855/1‑133 (MQ=255) ttCCCTCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACGAACACTTTTACTGTAACAGCACAGTGTCactg > 1:107466/1‑136 (MQ=255) ttCCCTCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACGAACACTTTTACTGTAACAGCACAGTGTCAtt > 2:188934/1‑138 (MQ=255) ttCCCTCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACGAACACTTTTACTGTAACAGCACAGTGTCAtt > 8:178456/1‑138 (MQ=255) ttCCCTCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACGAACACTTTTACTGTAACAGCACAGTGTCAtt > 2:81179/1‑138 (MQ=255) ttATCACTTTTGGCCTTTTTGGTCATGCTTTTTTACATAAAAAATCCGGCGGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACGAACACTTTTACTGTAACAGCACAGTGTCATTTATTCAAAATAGCaaa < 2:221588/139‑1 (MQ=255) | TATTTCTCCTCCTCTTTGCCATCAATGTCCGATTGTTTTATCTTATGTTGTCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCGCGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACGAACACTTTTACTGTAACAGCACAGTGTCATTTATTCAAAATAGCAAA > CP006881/2963813‑2964081 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |