Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 3,203,070 C→T P77P (CCG→CCA)  U712_16485 ← putative serine protease yvtA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068813,203,0700CT97.5% 138.0 / NA 40P77P (CCG→CCA) U712_16485putative serine protease yvtA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (18/21);  minor base G (1/0);  total (19/21)
Fisher's exact test for biased strand distribution p-value = 4.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCGGCGCCGTTGTAAAATTATCAGACTGCACCTGTTTATTTGCGGAAGCGGTTTCGGTCGCCTGATTTTGGTCTGACGGCAGATAAGGCGCGATGCCAAGCATGAGGCCCCCGCCGATAATACCGCCTAAAATCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGT‑TTCTTGCTTTTCCATTTCTAAAGCAGAAGCCGTTTCCTGCCGTCCCGCTTCATACGACACAGGCGCGTCAAGCAATTCTTGTT  >  CP006881/3202937‑3203200
                                                                                                                                     |                                                                                                                                   
tCGGCGCCGTTGTAAAATTATCAGACTGCACCTGTTTATTTGCGGAAGCGGTTTCGGTCGCCTGATTTTGGTCTGACGGCAGATAAGGCGCGATGCCAAGCATGAGGCCCCCGCCGATAATACCGCCTAAAATTGGGCt                                                                                                                                <  3:53957/139‑1 (MQ=255)
tCGGCGCCGTTGTAAAATTATCAGACTGCACCTGTTTATTTGCGGAAGCGGTTTCGGTCGCCTGATTTTGGTCTGACGGCAGATAAGGCGCGATGCCAAGCATGAGGCCCCCGCCGATAATACCGCCTAAAATTGGGCt                                                                                                                                <  3:190906/139‑1 (MQ=255)
  ggCGCCGTTGTAAAATTATCAGACTGCACCTGTTTATTTGCGGAAGCGGTTTCGGTCGCCTGATTTTGGTCTGACGGCAGATAAGGCGCGATGCCAAGCATGAGGCCCCCGCCGATAATACCGCCTAAAATTGGGCTc                                                                                                                               <  3:329587/138‑1 (MQ=255)
   tcgcCGTTGTAACATTATCAGACTGCACCTGTTTAATTGCGGAAGCGGTTTCGGGCGCCTGATTTTGGTCAGACGGCATATAAGGCGCGATGCCAAGCATGAGGCCCCCGCCGATAATTCCGCCTAAAATTGGGCTCAg                                                                                                                             <  6:106537/138‑1 (MQ=255)
   gcgcCGTTGTAAAATTATCAGACTGCACCTGTTTATTTGCGGAAGCGGTTTCGGTCGCCTGATTTTGGTCTGACGGCAGCTAAGGCGCGATGCCAAGCATGAGGCCCCCGCCGATAATACCGCCTAAAATTGGGCTCAg                                                                                                                             >  6:337824/1‑139 (MQ=255)
      ccGTTGTAAAATTATCAGACTGCACCTGTTTATTTGCGGAAGCGGTTTCGGTCGCCTGATTTTGGTCTGACGGCAGATAAGGCGCGATGCCAAGCATGAGGCCCCCGCCGATAATACCGCCTAAAATTGGGCTCAGCCa                                                                                                                          <  4:186308/139‑1 (MQ=255)
      ccGTTGTAAAATTATCAGACTGCACCTGTTTATTTGCGGAAGCGGTTTCGGTCGCCTGATTTTGGTCTGACGGCAGATAAGGCGCGATGCCAAGCATGAGGCCCCCGCCGATAATACCGCCTAAAATTGGGCTCAGCCa                                                                                                                          <  2:292883/139‑1 (MQ=255)
      ccGTTGTAAAATTATCAGACTGCACCTGTTTATTTGCGGAAGCGGTTTCGGTCGCCTGATTTTGGTCTGACGGCAGATAAGGCGCGATGCCAAGCATGAGGCCCCCGCCGAGTATACCGCCTAAAATGGGGCTCCGc                                                                                                                            >  4:241647/1‑137 (MQ=255)
                   aTCAGACTGCACCTGTTTATTTGCGGAAGCGGTTTCGGTCGCCTGATTTTGGTCTGACGGCAGATAAGGCGCGATGCCAAGCATGAGGCCCCCGCCGATAATACCGCCTAAAATTGTGCTCAGCCATGCGGCTCTCCGt                                                                                                             >  3:285725/1‑139 (MQ=255)
                   aTCAGACTGCACCTGTTTATTTGCGGAAGCGGTTTCGGTCGCCTGATTTTGGTCTGACGGCAGATAAGGCGCGATGCCAAGCATGAGGCCCCCGCCGATAATACCGCCTAAAATTGGGCTCAGCCATGCGGCTCTCCGt                                                                                                             >  3:161658/1‑139 (MQ=255)
                   aTCAGACTGCACCTGTTTATTTGCGGAAGCGGTTTCGGTCGCCTGATTTTGGTCTGACGGCAGATAAGGCGCGATGCCAAGCATGAGGCCCCCGCCGATAATACCGCCTAAAATTGGGCTCAGCCATGCGGCTCTCCGt                                                                                                             >  1:10689/1‑139 (MQ=255)
                   aTCAGACTGCACCTGTTTATTTGCGGAAGCGGTTTCGGTCGCCTGATTTTGGTCTGACGGCAGATAAGGCGCGATGCCAAGCATGAGGCCCCCGCCGATAATACCGCCTAAAATTGGGCTCAACCATGCGGCTCTCCGt                                                                                                             >  8:87295/1‑139 (MQ=255)
                                  tttATTTGCGGAAGCGGTTTCGGTCGCCTGATTTTGGTCTGACGGCAGATAAGGCGCGATGCCAAGCATGAGGCCCCCGCCGATAATACCGCCTAAAATTGGGCTCAGCCCTGCCGCTCTCCGGTTCGTTTCtttttt                                                                                               >  5:64659/1‑138 (MQ=255)
                                    tATTTGCGGAAGCGGTTTCGGTCGCCTGATTTTGGTCTGACGGCAGATAAGGCGCGATGCCAAGCATGAGGCCCCCGCCGATAATACCGCCTAAAATTGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTca                                                                                             >  8:13666/1‑138 (MQ=255)
                                      tttGCGGAAGCGGTTTCGGTCGCCTGATTTTGGTCTGACGGCAGATAAGGCGCGATGCCAAGCATGAGGCCCCCGCCGATAATACCGCCTAAAATTGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAg                                                                                          <  7:281021/139‑1 (MQ=255)
                                         gCGGAAGCGGTTTCGGTCGCCTGATTTTGGTCTGACGGCAGATAAGGCGCGATGCCAAGCATGAGGCCCCCGCCGATAATACCGCCTAAAATTGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCg                                                                                       >  7:60267/1‑139 (MQ=255)
                                                       gggcGCCTGATTTTGGTCTGACGGCAGATAAGGCGCGATGCCAAGCATGAGGCCCCCGCCGATAATACCGCCTAAAATTGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGT‑TTCTTGCTTTTcc                                                                        <  1:167564/136‑1 (MQ=255)
                                                       ggTCGCCTGATTTTGGTCTGACGGCAGATAAGGCGCGATGCCAAGCATGAGGCCCCCGCCGATAATACCGCCTAAAATTGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGT‑TTCTTGCTTTTcc                                                                        <  5:171712/139‑1 (MQ=255)
                                                       ggTCGCCTGATTTTGGTCTGACGGCAGATAAGGCGCGATGCCAAGCATGAGGCCCCCGCCGATAATACAGCCTAAAATTGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGT‑TTCTTGCTTTTc                                                                         <  4:285725/138‑1 (MQ=255)
                                                         tCGCCTGATTTTGGTCTGACGGCAGATAAGGCGCGATGCCAAGCATGAGGCCCCCGCCGATAATACCGCCTAAAATTGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGT‑TTCTTGCTTTTCCAt                                                                      <  2:10689/139‑1 (MQ=255)
                                                           gCCTGATTTTGGTCTGACGGCAGATAAGGCGCGATGCCAAGCATGAGGCCCCCGCCGATAATACCGCCTAAAATTGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGT‑TTCTTGCTTTTCCAttt                                                                    >  4:332585/1‑139 (MQ=255)
                                                                aTTTTGGTCTGACGGCAGATAAGGCGCGATGCCAAGCATGAGGCCCCCGCCGATAATACCGCCTAAAATTGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGT‑TTCTTGCTTTTCCATTTCTa                                                                 <  6:60739/137‑1 (MQ=255)
                                                                       tCTGACGGCAGATAAGGCGCGATGCCAAGCATGAGGCCCCAGCCGATAATACAGCCTAAAATTGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGT‑TTCTTTCTTTTCCATTTCTAAAGCAGAAg                                                        <  7:364869/139‑1 (MQ=255)
                                                                            cGGCAGATAAGGCGCGATGCCAAGCATGAGGCCCCCGCCGATAATAACGCCTAAAATTGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGT‑TTCTTGCTTTTCCATTTCTAAAGCAGAAGCCGtt                                                   <  6:358714/139‑1 (MQ=255)
                                                                                     aGGCGCGATGCCAAGCATGAGGCCCCCGCCGATAATACCGCCTAAAATTGGGCt                                                                                                                                >  6:205397/1‑54 (MQ=255)
                                                                                     aGGCGCGATGCCAAGCATGAGGCCCCCGCCGATAATACCGCCTAAAATTGGGCt                                                                                                                                <  5:205397/54‑1 (MQ=255)
                                                                                      ggCGCGATGCCAAGCATGAGGCCCCCGCCGATAATACCGCCTAAAATTGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGT‑TTCTTGCTTTTCCATTTCTAAAGCAGAAGCCGTTTCCTGCCGTc                                         <  3:332585/139‑1 (MQ=255)
                                                                                         gcgATGCCAAGCATGAGGCCCCCGCCGATAATACCGCCTAAAATTGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGT‑TTCTTGCTTTTCCATTTCTAAAGCAGAAGCCGTTTCCTGCCGTCCCg                                      <  4:161658/139‑1 (MQ=255)
                                                                                         gcgATGCCAAGCATGAGGCCCCCGCCGATAATACCGCCTAAAATTGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGT‑TTATTTGGTTTACTATTCTAAAGAAGAAGCCGGTTGCTGCCGTCCCg                                      >  8:369448/1‑139 (MQ=255)
                                                                                           gATGCCAAGCATGAGGCCCCAGCCGATAATACCGCCTAAAATTGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGT‑TTCTTGCTTTTCCATTTCTAAAGCAGAAGCCGTTTCCTGCCGTCCCGCt                                    <  7:369448/139‑1 (MQ=255)
                                                                                            aTGCCAAGCATGAGGCCCCCGCCGATAATACCGCCTAAAATTGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGT‑TTATTGCTTTTACATTTATAAAGCAGAAGCCGTTTACTGCCGTCCCGcg                                    >  8:37002/1‑137 (MQ=255)
                                                                                                cAAGCATGAGGCCCCCGCCGATAATACCGCCTAAAATTGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGT‑TTCTTGCTTTTCCATTTCTAAAGCAGAAGCCGTTTCCTGCCGTCCCGCTTCATa                               <  3:37806/139‑1 (MQ=255)
                                                                                                cAAGCATGAGGCCCCCGCCGATAATACCGCCTAAAATTGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGT‑TTCTTGCTTTTCCATTTCTAAAGCAGAAGCCGTTTCCTGCCGTCCCGCTTCATa                               <  7:13666/139‑1 (MQ=255)
                                                                                                cAAGCATGAGGCCCCCGCCGATAATACCGCCTAAAATTGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGT‑TTCTTGCTTTTCCATTTCTAAAGCAGAAGCCGTTTCCTGCCGTCCCGCTTCATa                               <  7:37002/139‑1 (MQ=255)
                                                                                                  aGCATGAGGCCCCCGCCGATAATACCGCCTAAAATTGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGT‑GTGTTGGGTTTGGATTTGTTAAGAATCAGCCGGTTCCTGGAGTGCCGCGTcgatag                             >  7:334026/1‑134 (MQ=255)
                                                                                                              ccgccgATAATACCGCCTAAAATTGGGCTCAGCCATGCGGCTCTCCGTTTCGTTTCTTTTTTCACAGCCGTGTGGTTGGTTTT‑CATTTGTAAAGCAGAATCCGGTTGCTGCCCGCCCGCTTCATACGACACACTcgcgg                 >  4:139647/1‑138 (MQ=255)
                                                                                                                        taCCGCCTAAAATTGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGT‑TTGTTTGTTTTGCATTTCTAAAGCAGAAGCCGGTTCCTGCCGTCCCGCGTGATACGCCACAGGCGCGGCAAGccaatg       >  5:334111/1‑134 (MQ=255)
                                                                                                                           cGCCTAAAATTGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGT‑GTGTTGGTTTTTCATTTCTAAAGCAGAAGCCGGTTCCAGCCGTCCCGCGTCATTCGACACCGGCGCGTCAAGCAAttgttg    >  3:365940/1‑139 (MQ=255)
                                                                                                                           cGCCTAAAATTGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTGTTTCACAGCCGT‑TTCTTGGTTTTCCCTTTGATAACCCGAAGCCGTTTCCGGCCGTCCCGCGTCCTACGACACAGGCGCCGCCAGCCATTCTTg    >  1:205670/1‑139 (MQ=255)
                                                                                                                             ccTAAAATTGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGT‑TTGTTGGTTTTGCATTTGTAAAGCAGAAGCCGGTTACTGGCGTCCCGCGTCATTCGAAACAGGGGCGTGAATCAAATgttgtt  >  5:25803/1‑139 (MQ=255)
                                                                                                                                     |                                                                                                                                   
TCGGCGCCGTTGTAAAATTATCAGACTGCACCTGTTTATTTGCGGAAGCGGTTTCGGTCGCCTGATTTTGGTCTGACGGCAGATAAGGCGCGATGCCAAGCATGAGGCCCCCGCCGATAATACCGCCTAAAATCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGT‑TTCTTGCTTTTCCATTTCTAAAGCAGAAGCCGTTTCCTGCCGTCCCGCTTCATACGACACAGGCGCGTCAAGCAATTCTTGTT  >  CP006881/3202937‑3203200

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑