BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	pSIJ663_pHT315_araE_xy	2,039	C→T	intergenic (–/‑939)	– / → eryR	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	pSIJ663_pHT315_araE_xy	2,039	0	C	T	98.8%	10354.7 / 58.4	3203	intergenic (–/‑939)	–/eryR	–/–
Reads supporting (aligned to +/- strand): ref base C (21/19); new base T (1725/1425); total (1754/1449)
Fisher's exact test for biased strand distribution p-value = 8.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.45e-01

Only 100 of 3248 total aligned reads displayed.

CTTGGGTCCAACTTTATAGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTCATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTA  >  pSIJ663_pHT315_araE_xy/1903‑2173

   gggTCCAACTTTATAGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATaaa                                                                                                                                   <  8:327282/139‑1 (MQ=255)

        cAACTTTATAGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATATTTTATTTTCGATGAATATTTTTTATAAATaa                                                                                                                                >  4:245001/1‑136 (MQ=255)

         aaCTTTATAGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAg                                                                                                                             <  6:76799/139‑1 (MQ=255)

               ataGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGAtgtt                                                                                                                       >  4:215165/1‑139 (MQ=255)

               ataGCAAATAGTATATGGTCATTTAAAATCTTTAAAAAAAAAAATCTAATAGGTTCTTTAGGATTTTGCCCTACATAGTCGGGGTGTTAAACGATATCTTTTATGTGAGATTAATATTTTTTATAAATACAAAGAtgtt                                                                                                                       >  8:280064/1‑139 (MQ=255)

                taGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGAtgttg                                                                                                                      >  1:274645/1‑139 (MQ=255)

                     aTTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATaaa                                                                                                                                   <  5:212371/121‑1 (MQ=255)

                     aTTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATa                                                                                                                                 <  6:298464/123‑1 (MQ=255)

                     aTTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCt                                                                                                                 >  5:45404/1‑139 (MQ=255)

                     aTTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGGTGTTTCt                                                                                                                 >  6:197243/1‑139 (MQ=255)

                     aTTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGAGATGAATATTTTTTATAAATACCAGGATGTTGTTTCt                                                                                                                 >  8:40392/1‑139 (MQ=255)

                          tataTGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACg                                                                                                            <  6:338878/139‑1 (MQ=255)

                            tatGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTg                                                                                                          <  5:308977/139‑1 (MQ=255)

                            tatGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTg                                                                                                          <  1:200536/139‑1 (MQ=255)

                            tatGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTg                                                                                                          <  2:312692/139‑1 (MQ=255)

                             atGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGc                                                                                                         >  1:295416/1‑139 (MQ=255)

                              tGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGATTATTTACGTGCt                                                                                                        >  2:211826/1‑139 (MQ=255)

                                   aTTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTtat                                                                                                    >  6:167535/1‑138 (MQ=255)

                                    tttAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGTTTTTGCCCGACATAGTCGGGGTGTTAAACGATATCTTTTATGTGCGATGAATATTTTTTATAAAGACCAGGATGTTGTTTCTATAAGTGCTTtata                                                                                                   >  6:223497/1‑138 (MQ=255)

                                    tttAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTTCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCGTTACGTGCTTtata                                                                                                   >  8:147263/1‑138 (MQ=255)

                                    tttAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATTAATATTTTTTATTAATACCAGGATGTTGTTTCTTTACGGGCTTtatt                                                                                                   >  8:17312/1‑137 (MQ=255)

                                    tttAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTtata                                                                                                   >  7:108548/1‑138 (MQ=255)

                                    tttAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTtata                                                                                                   >  1:224760/1‑138 (MQ=255)

                                    tttAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTtata                                                                                                   >  2:66188/1‑138 (MQ=255)

                                    tttAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTtata                                                                                                   >  8:35231/1‑138 (MQ=255)

                                    tttAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTtata                                                                                                   >  2:142162/1‑138 (MQ=255)

                                    tttAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTtata                                                                                                   >  6:316876/1‑138 (MQ=255)

                                    tttAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGAGGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTtata                                                                                                   >  4:290383/1‑138 (MQ=255)

                                             tttACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGa                                                                                          >  3:300745/1‑138 (MQ=255)

                                               tACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGaaa                                                                                        <  3:245001/138‑1 (MQ=255)

                                               tACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGaaa                                                                                        <  3:87505/138‑1 (MQ=255)

                                               tACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGaaa                                                                                        <  8:259221/138‑1 (MQ=255)

                                                aCCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTTTTTCTTTTCGTGCTGTTTAAATGCGGGAAACa                                                                                      >  2:256135/1‑139 (MQ=255)

                                                     ttCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCTGGAAACAtttt                                                                                  >  5:262114/1‑138 (MQ=255)

                                                        aaCGCTATTAGGTTCTTTAGGATTTTGCGCGACATATTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTAc                                                                               <  2:348438/138‑1 (MQ=255)

                                                        aaCGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGATACATTTTTAc                                                                               <  2:234245/138‑1 (MQ=255)

                                                        aaCGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTAc                                                                               <  5:67241/138‑1 (MQ=255)

                                                        aaCGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTAc                                                                               <  3:130918/138‑1 (MQ=255)

                                                        aaCGCTATTAGGTGCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACa                                                                              <  1:103209/139‑1 (MQ=255)

                                                        aaCGCTATTAGGGTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTAc                                                                               <  1:324358/138‑1 (MQ=255)

                                                        aaCGCTATTACGATCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTAc                                                                               <  6:272897/138‑1 (MQ=255)

                                                          cGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATc                                                                            >  1:41696/1‑139 (MQ=255)

                                                               ttAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTa                                                                                                              <  6:250884/100‑1 (MQ=255)

                                                                     tCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTg                                                                  >  3:59464/1‑138 (MQ=255)

                                                                      cTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGc                                                                 >  7:368228/1‑138 (MQ=255)

                                                                      cTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGc                                                                 <  7:282435/138‑1 (MQ=255)

                                                                      cTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGc                                                                 <  4:170612/138‑1 (MQ=255)

                                                                      cTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGc                                                                 >  3:38185/1‑138 (MQ=255)

                                                                       tttAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGa                                                                                          >  4:193036/1‑112 (MQ=255)

                                                                       tttAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGcaa                                                               >  3:101738/1‑139 (MQ=255)

                                                                       tttAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGc                                                                 >  1:351444/1‑137 (MQ=255)

                                                                       tttAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGc                                                                 >  5:363330/1‑137 (MQ=255)

                                                                       tttAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGc                                                                 >  5:28451/1‑137 (MQ=255)

                                                                       tttAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGc                                                                 >  8:10440/1‑137 (MQ=255)

                                                                       tttAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGc                                                                 >  5:148876/1‑137 (MQ=255)

                                                                       tttAGGATTTTGCACGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTCATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGc                                                                 >  5:334120/1‑137 (MQ=255)

                                                                       tttAGGACTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGAtgtt                                                                                                                       <  4:314737/83‑1 (MQ=255)

                                                                          aGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGcaagt                                                             <  7:77945/138‑1 (MQ=255)

                                                                          aGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGcaagt                                                             <  5:96770/138‑1 (MQ=255)

                                                                          aGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGcaagt                                                             <  7:160997/138‑1 (MQ=255)

                                                                           ggATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGcaagtca                                                           <  7:53067/139‑1 (MQ=255)

                                                                           ggATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGcaagtc                                                            <  5:233349/138‑1 (MQ=255)

                                                                           ggATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGcaagtc                                                            <  4:375823/138‑1 (MQ=255)

                                                                                 tGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTta                                                      >  5:11472/1‑138 (MQ=255)

                                                                                   cccGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTtata                                                    <  4:146660/138‑1 (MQ=255)

                                                                                    ccGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATg                                                  <  3:210313/139‑1 (MQ=255)

                                                                                    ccGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATg                                                  <  4:86632/139‑1 (MQ=255)

                                                                                     cGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGt                                                 <  3:158148/139‑1 (MQ=255)

                                                                                     cGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGt                                                 <  8:301539/139‑1 (MQ=255)

                                                                                     cGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGt                                                 <  3:181421/139‑1 (MQ=255)

                                                                                     cGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGt                                                 <  6:366796/139‑1 (MQ=255)

                                                                                     cGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGt                                                 <  4:59464/139‑1 (MQ=255)

                                                                                      gACATAGTCGGGGTGGTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTGTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTa                                                >  4:108602/1‑139 (MQ=255)

                                                                                       aCATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTAt                                               <  2:369729/139‑1 (MQ=255)

                                                                                          tAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCta                                            <  8:232109/139‑1 (MQ=255)

                                                                                           aGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCta                                            >  8:73170/1‑138 (MQ=255)

                                                                                               ggggTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCATGTCAAGTTATATGTATCTATAATg                                       <  7:308278/139‑1 (MQ=255)

                                                                                                  gtgtTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGt                                                 >  8:129315/1‑126 (MQ=255)

                                                                                                  gtgtTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGt                                                 >  1:141780/1‑126 (MQ=255)

                                                                                                  gtgtTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGAtt                                    >  3:351500/1‑139 (MQ=255)

                                                                                                     ttCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTtgt                                 >  1:171779/1‑139 (MQ=255)

                                                                                                       cAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTtgtg                                >  7:197905/1‑138 (MQ=255)

                                                                                                       cAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTtgtg                                <  3:329494/138‑1 (MQ=255)

                                                                                                       cAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTgg                               >  6:129531/1‑139 (MQ=255)

                                                                                                             tatCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGcaagt                                                             <  5:176908/103‑1 (MQ=255)

                                                                                                             tatCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGttt                         <  2:167163/139‑1 (MQ=255)

                                                                                                             tatCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGttt                         >  2:190341/1‑139 (MQ=255)

                                                                                                             tatCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGttt                         >  2:28692/1‑139 (MQ=255)

                                                                                                               tCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGt                                                 <  1:24777/113‑1 (MQ=255)

                                                                                                               tCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTg                       >  7:129823/1‑139 (MQ=255)

                                                                                                                  tttATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTATACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCca                    >  3:270745/1‑139 (MQ=255)

                                                                                                                    tATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTAt                                               <  7:233572/110‑1 (MQ=255)

                                                                                                                    tATGTGCGATGAATATTTTTTATAAATAACAGTATGTTGGTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCcac                   <  4:341175/138‑1 (MQ=255)

                                                                                                                       gtgCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCacaa                 >  4:266637/1‑137 (MQ=255)

                                                                                                                        tgCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAg              <  2:108922/139‑1 (MQ=255)

                                                                                                                         gCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGt             >  7:329987/1‑139 (MQ=255)

                                                                                                                          cGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGtt                                                       <  6:52311/96‑1 (MQ=255)

                                                                                                                          cGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGtt            >  8:201535/1‑139 (MQ=255)

                                                                                                                          cGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGtt            <  7:260204/139‑1 (MQ=255)

                                                                                                                               aaTATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGtt        <  1:74054/138‑1 (MQ=255)

CTTGGGTCCAACTTTATAGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTCATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTA  >  pSIJ663_pHT315_araE_xy/1903‑2173

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`