Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	924,079	T→A	intergenic (‑59/‑220)	U712_04490 ← / → U712_04495	Vegetative catalase/Aliphatic sulfonates import ATP‑binding protein SsuB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	924,079	0	T	A	100.0%	116.6 / NA	34	intergenic (‑59/‑220)	U712_04490/U712_04495	Vegetative catalase/Aliphatic sulfonates import ATP‑binding protein SsuB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (15/19);  total (15/19)

CAGTCATTGAGTTTTGATTATCTCCAACCGGAGCGCCCCAGCTAGTTGTCAGTTTATTTGAACTCATGTTATCACCTCTTGGAATTTATAAGAACATAATATCATCTCTAAGTAAAAAGTCAATATTATTTTATAATTATTATAAAATAGTTATCTCTATTAATCGATATATAGTATCGAAATTGTTGAAAAACCTTGTTGTAACAGCTTTTTATGACACTTAATAATTTTTGGAGAAATAATTTTTCCTTCTCTATTTGC  >  CP006881/923954‑924214                                                                                                                              |                                                                                                                                        cAGTCATTGAGTTTTGATTCTCTCCAACCGGAGCGCCCCAGCTAGTTGTCAGTTTATTTGAACTCATGTTATCACCTCTTGGAATTTATAAGAACATAATATCATCTCTAAGTAAAAAGTCAATAATATTTTATAatta                                                                                                                            <  2:348836/139‑1 (MQ=255) cAGTCATTGAGTTTTGATTATCTCCAACCGGAGCGCCCCAGCTAGTTGTCAGTTTATTTGAACTCATGTTATCACCTCTTGGAATTTATAAGAACATAATATCATCTCTAAGTAAAAAGTCAATAATATTTTATAatta                                                                                                                            <  2:241949/139‑1 (MQ=255)           gTTTTGATTATCTCCAACCGGAGCGCCCCAGCTAGTTGTCAGTTTATTTGAACTCATGTTATCACCTCTTGGAATTTATAAGAACATAATATCATCTCTAAGTAAAAAGTCAATAATATTTTATAATTATTATAAAATa                                                                                                                  <  6:280237/139‑1 (MQ=255)             tttGATTATCTCCAACCGGAGCGCCCCAGCTAGTTGTCAGTTTATTTGAACTCATGTTATCACCTCTTGGAATTTATAAGAACATAATATCATCTCTAAGTAAAAAGTCAATAATATTTTATAATTATTATAAAATAGt                                                                                                                >  1:171426/1‑139 (MQ=255)                 aTTATCTCCAACCGGAGCGCCCCAGCTAGTTGTCAGTTTATTTGAACTCATGTTATCACCTCTTGGAATTTATAAGAACATAATATCATCTCTAAGTAAAAAGTCAATAATATTTTATAATTATTATAAAATAGTTAt                                                                                                             <  8:368631/138‑1 (MQ=255)                 aTTATCTCCAACCGGAGCGCCCCAGCTAGTTGTCAGTTTATTTGAACTCATGTTATCACCTCTTGGAATTTATAAGAACATAATATCATCTCTAAGTAAAAAGTCAATAATATTTTATAATTATTATAAAATAGTTAt                                                                                                             <  5:364390/138‑1 (MQ=255)                 aTTATCTCCAACCGGAGCGCCCCAGCTAGTTGTCAGTTTATTTGAACTCATGTTATCACCTCTTGGAATTTATAAGAACATAATATCATCTCTAAGTAAAAAGTCAATAATATTTTATAATTATTATAAAATAGTTAt                                                                                                             <  4:258829/138‑1 (MQ=255)                 aTTATCTCCAACCGGAGCGCCCCAGCTAGTTGTCAGTTTATTTGAACTCATGTTATCACCTCTTGGAATTTATAAGAACATAATATCATCTCTAAGTAAAAAGTCAATAATATTTTATAATTATTATAAAATAGTTAt                                                                                                             <  3:304447/138‑1 (MQ=255)                 aTTATCTCCAACCGGAGCGCCCCAGCTAGTTGTCAGTTTATTTGAACTCATGTTATCACCTCTTGGAATTTATAAGAACATAATATCATCTCTAAGTAAAAAGTCAATAATATTTTATAATTATTATAAAATAGTTAt                                                                                                             <  3:264454/138‑1 (MQ=255)                      ctcCAACCGGAGCGCCCCAGCTAGTTGTCAGTTTATTTGAACTCATGTTATCACCTCTTGGAATTTATAAGAACATAATATCATCTCTAAGTAAAAAGTCAATAATATTTTATAATTATTATAAAATAGTTATCTCTAt                                                                                                       >  7:268396/1‑139 (MQ=255)                         cAACCGGAGCGCCCCAGCTAGTTGTCAGTTTATTTGAACTCATGTTATCACCTCTTGGAATTTATAAGAACATAATATCATCTCTAAGTAAAAAGTCAATAATATTTTATAATTATTATAAAATAGTTATCTCTAtt                                                                                                      <  4:154396/137‑1 (MQ=255)                          aaCCGGAGCGCCCCAGCTAGTTGTCAGTTTATTTGAACTCATGTTATCACCTCTTGGAATTTATAAGAACATAATATCATCTCTAAGTAAAAAGTCAATAATATTTTATAATTATTATAAAATAGTTATCTCTATTAAt                                                                                                   <  7:24750/139‑1 (MQ=255)                          aaCCGGAGCGCCCCAGCTAGTTGTCAGTTTATTTGAACTCATGTTATCACCTCTTGGAATTTATAAGAACATAATATCATCTCTAAGTAAAAAGTCAATAATATTTTATAATTATTATAAAATAGTTATCTCTATTAAt                                                                                                   <  5:73820/139‑1 (MQ=255)                          aaCCGGAGCGCCCCAGCTAGTTGTCAGTTTATTTGAACTCATGTTATCACCTCTTGGAATTTATAAGAACATAATATCATCTCTAAGTAAAAAGTCAATAATATTTTATAATTATTATAAAATAGTTATCTCTATTAAt                                                                                                   <  1:149319/139‑1 (MQ=255)                          aaCCGGAGCGCCCCAGCTAGTTGTCAGTTTATTTGAACTCATGTTATCACCTCTTGGAATTTATAAGAACATAATATCATCTCTAAGTAAAAAGTCAATAATATTTTATAATTATTATAAAATAGTTATCTCTATTAAt                                                                                                   <  5:323041/139‑1 (MQ=255)                          aaCCGGAGCGCCCCAGCTAGTTGTCAGTTTATTTGAACTCATGTTATCACCTCTTGGAATTTATAAGAACATAATATCATCTCTAAGTAAAAAGTCAATAATATTTTATAATTATTATAAAATAGTTATCTCTATTAAt                                                                                                   <  8:268396/139‑1 (MQ=255)                          aaCCGGAGCGCCCCAGCTAGTTGTCAGTTTATTTGAACTCATGTTATCACCTCTTGGAATTTATAAGAACATAATATCATCTCTAAGTAAAAAGTCAATAATATTTTATAATTATTATAAAATAGTTATCTCTATTAAt                                                                                                   <  2:171426/139‑1 (MQ=255)                          aaCCGGAGCGACCCAGCTAGTTGTCAGTTTATTTGAACTCATGTTATCACCTCTTGGAATTTATAAGAACATAATATCATCTCTAAGTAAAAAGTCAATAATATTTTATAATTATTATAAAATAGTTATCTCTATTAAt                                                                                                   <  1:185546/139‑1 (MQ=255)                            ccGGAGCGCCCCAGCTAGTTGTCAGTTTATTTGAACTCATGTTATCACCTCTTGGAATTTATAAGAACATAATATCATCTCTAAGTAAAAAGTCAATAATATTTTATAATTATTATAAAATAGTTATCTCTATTAATCg                                                                                                 <  3:247233/139‑1 (MQ=255)                                aGCGCCCCAGCTAGTTGTCAGTTTATTTGAACTCATGTTATCACCTCTTGGAATTTATAAGAACATAATATCATCTCTAAGTAAAAAGTCAATAATATTTTATAATTATTATAAAATAGTTAt                                                                                                             >  1:102588/1‑123 (MQ=255)                                aGCGCCCCAGCTAGTTGTCAGTTTATTTGAACTCATGTTATCACCTCTTGGAATTTATAAGAACATAATATCATCTCTAAGTAAAAAGTCAATAATATTTTATAATTATTATAAAATAGTTAt                                                                                                             <  2:102588/123‑1 (MQ=255)                                    ccccAGCTAGTTGTCAGTTTATTTGAACTCATGTTATCACCTCTTGGAATTTATAAGAACATAATATCATCTCTAAGTAAAAAGTCAATAATATTTTATAATTATTATAAAATAGTTATCTCTATTAAt                                                                                                   <  5:65748/129‑1 (MQ=255)                                    ccccAGCTAGTTGTCAGTTTATTTGAACTCATGTTATCACCTCTTGGAATTTATAAGAACATAATATCATCTCTAAGTAAAAAGTCAATAATATTTTATAATTATTATAAAATAGTTATATCTATTAAt                                                                                                   >  6:65748/1‑129 (MQ=255)                                               tGTCAGTTTATTTGAACTCATGTTATCACCTCTTGGAATTTATAAGAACATAATATCATCTCTAAGTAAAAAGTCAATAATATTTTATAATTATTATAAAATAGTTATCTCTATTAATCGATATATAGTATCGAAAttg                                                                              >  1:5437/1‑139 (MQ=255)                                                               cTCATGTTATCACCTCTTGGAATTTATAAGAACATAATATCATCTCTAAGTAAAAAGTCAATAATATTTTATAATTATTATAAAATAGTTATCTCTATTAATCGATATATAGTATCGAAATTGTTGAAAAACCttgttg                                                              >  1:287280/1‑139 (MQ=255)                                                               cTCATGTTATCACCTCTTGGAATTTATAAGAACATAATATCATCTCTAAGTAAAAAGTCAATAATATTTTATAATTATTATAAAATAGTTATCTCTATTAATCGATATATAGTATCGAAATTGTTGAAAAAACttgttg                                                              >  7:282947/1‑139 (MQ=255)                                                                               ttGGAATTTATAAGAACATAATATCATCTCTAAGTAAAAAGTCAATAATATTTTATAATTATTATAAAATAGTTATCTCTATTAATCTATATATAGTATCGAAATTGTTGAAAAACCTTGTTTTAACAGCTTTTAATg                                               >  3:49923/1‑138 (MQ=255)                                                                                             aaCATAATATCATCTCTAAGTAAAAAGTCAATAATATTTTATAATTATTATAAAATAGTTATCTCTATTAATCGATATATAGTATCGAAATTGTTGAAAAACCTTGTTGTAACAGCTTTTTATGACACTTAATAAtttt                                >  7:6531/1‑139 (MQ=255)                                                                                                       catcTCTAAGTAAAAAGTCAATAATATTTTATAATTATTATAAAATAGTTATCTCTATTAATCGATATATAGTATCGAAATTGTTGAAAAACCTTGTTGTAACAGCTTTTTATGACACTTAATAATTTTTGGAGAaat                       >  5:371846/1‑138 (MQ=255)                                                                                                         tctcTAAGTAAAAAGTCAATAATATTTTATAATTATTATAAAATAGTTATCTCTATTAATCGATATATAGTATCGAAATTGTTGAAAAACCTTGTTGTAACAGCTTTTTATTACACTTAATAATTTTTGTAGAaataat                    >  3:52292/1‑139 (MQ=255)                                                                                                         tctcTAAGTAAAAAGTCAATAATATTTTATAATTATTATAAAATAGTTATCTCTATTAATCGATATATAGTATCGAAATTGTTGAAAAACCTTGTTGTAACAGCTTTTTATGACACTTAATAATTTTTGGAGAaataat                    >  2:154084/1‑139 (MQ=255)                                                                                                         tctcTAAGTAAAAAGTCAATAATATTTTATAATTATTATAAAATAGTTATCTCTATTAATCGATATATAGTATCGAAATTGTTGAAAAACCTTGTTGTAACAGCTTTTTATGACACTTAATAATTTTTGGAGAaataat                    >  1:278147/1‑139 (MQ=255)                                                                                                         tctcTAAGTAAAAAGTCAATAATATTTTATAATTATTATAAAATAGTTATCTCTATTAATCGATATATAGTATCGAAATTGTTGAAAAACCTTGTTGTAACAGCTTTTTATGACACTTAATAATTTTTGGAGAaataat                    >  1:12523/1‑139 (MQ=255)                                                                                                          ctctAAGTAAAAAGTCAATAATATTTTATAATTATTATAAAATAGTTATCTCTATTAATCGATATATAGTATCGAAATTGTTGAAAAACCTTGTTGTAACAGCTTTTTATGACACTTAATAATTTTTGGAGAAATAAtt                   >  3:224552/1‑139 (MQ=255)                                                                                                                           ataataTTTTATAATTATTATAAAATAGTTATCTCTATTAATCGATATATAGTATCGAAATTGTTGAAAAACCTTGTTGTAACAGCTTTTTATGACACTTAATAATTTTTGGAGAAATAATTTTTCCTTCTCTATTTGc  >  4:66716/1‑139 (MQ=255)                                                                                                                              |                                                                                                                                        CAGTCATTGAGTTTTGATTATCTCCAACCGGAGCGCCCCAGCTAGTTGTCAGTTTATTTGAACTCATGTTATCACCTCTTGGAATTTATAAGAACATAATATCATCTCTAAGTAAAAAGTCAATATTATTTTATAATTATTATAAAATAGTTATCTCTATTAATCGATATATAGTATCGAAATTGTTGAAAAACCTTGTTGTAACAGCTTTTTATGACACTTAATAATTTTTGGAGAAATAATTTTTCCTTCTCTATTTGC  >  CP006881/923954‑924214

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑