| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | CP006881 | 3,723,942 | C→G | L252L (CTC→CTG) | U712_19195 → | putative transporter ywcJ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 3,723,942 | 0 | C | G | 98.4% | 222.2 / NA | 64 | L252L (CTC→CTG) | U712_19195 | putative transporter ywcJ |
| Reads supporting (aligned to +/- strand): ref base C (0/1); major base G (38/24); minor base T (0/1); total (38/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.97e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CACATTCGCCATCTCACTTTTGATCGAGCACCCTGATACAGTGACAGTAATGGGAGCAGTCAGAAACTTAATTCCCGTTACACTCGGCAACCTGACCGCGGGAATGGTCATGATGGGCTGGATGTATTACA‑CGCTCAATCCCGATAAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGACTCCGGCAAATGTAATT > CP006881/3723807‑3724069 | cacaTTCGCCATCTCGCTTTTGATCGAGCCCCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACA‑CACTGAAt > 1:272933/1‑139 (MQ=255) caTTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACA‑CACTGAATcc > 8:109062/1‑139 (MQ=255) tCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACA‑CACTGAATCCTGa > 1:20815/1‑139 (MQ=255) tCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACA‑CACTGAATCCTGa > 7:326859/1‑139 (MQ=255) aTCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACA‑CACTGAATCCTGatcaa > 2:286235/1‑138 (MQ=255) gcTTTTGAGCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTCCGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACA‑CACTGAATCCTGATCAATaaaaa < 6:294265/138‑1 (MQ=255) aTCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACA‑CACTGAATCCTGATCAATAAAAAAACTTcc < 7:201039/139‑1 (MQ=255) ccTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGCATGTACTACA‑CACTGAATCCTGATCAATAAAAAAACTTCCAGAGATcct > 7:284717/1‑137 (MQ=255) cTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACA‑CACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACt < 8:326859/139‑1 (MQ=255) cTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACA‑CACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACt < 7:109062/139‑1 (MQ=255) ggATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACA‑CACTGAATCCTGATCAATAAAAAACCTTCCAGAGATCACTc > 2:345330/2‑139 (MQ=255) aCAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACA‑CACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTg < 3:268911/139‑1 (MQ=255) tGACACTGATGGGAGCAGTCAGAAACTTACTCCCCGTTACGCTCGGCAATCTGACCGCGGGAATATTTATGATGGGCTGGATGTACTACA‑AACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAg > 3:231447/1‑139 (MQ=255) tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGCCCGCGGGAATAGTTATGATGGGCTGGATGTACTACA‑CACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAg > 3:282417/1‑139 (MQ=255) tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACA‑CACTGAATCCTGatc > 2:222198/1‑104 (MQ=255) tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACA‑CACTGAATCCTGatc < 1:222198/105‑2 (MQ=255) tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACA‑CACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAg > 5:265471/1‑139 (MQ=255) tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACA‑CACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAg > 5:185268/1‑139 (MQ=255) tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACA‑CACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAg > 5:84796/1‑139 (MQ=255) tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACA‑CACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAg > 1:355442/1‑139 (MQ=255) tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACA‑CACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAg > 1:289414/1‑139 (MQ=255) tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACA‑CACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAg > 3:196820/1‑139 (MQ=255) acactgaTGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACA‑CACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACt < 7:304285/122‑1 (MQ=255) acactgaTGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACA‑CACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACt > 8:304285/7‑128 (MQ=255) acactgaTGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACA‑CACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGt > 8:204182/7‑138 (MQ=255) acactgaTGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACA‑CACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTc > 7:45859/7‑139 (MQ=255) acactgaTGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTAATACA‑CACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGt > 3:102198/7‑138 (MQ=255) aTGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACA‑CACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACt > 2:199369/1‑122 (MQ=255) aTGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACA‑CACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAg < 4:183982/137‑1 (MQ=255) aTGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACA‑CACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAg < 6:84796/137‑1 (MQ=255) aTGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACA‑CACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCg > 1:3957/1‑139 (MQ=255) aTGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACA‑CACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCg > 5:266870/1‑139 (MQ=255) aTGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGAAATAGTTATGATGGGCTGGATGTACTACA‑CACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACt < 1:199369/122‑1 (MQ=255) aGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACA‑CACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTAt > 5:161323/1‑139 (MQ=255) aGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACA‑CACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTAt > 7:26878/1‑139 (MQ=255) tCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACA‑CACTGAATCCTGATCAATAAAAAAACTTCCATAGATCACTCCCTGCAAGTCAAAGCGGTTAtt < 7:113758/135‑1 (MQ=255) tCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACA‑CACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTAtt > 8:113758/1‑135 (MQ=255) aGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACA‑CACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCgaga < 1:286235/139‑1 (MQ=255) aaaccccGTTACGCTCGGCAATCTGACCGCGTTAATTGTTATTATGGGCGTGATGTAATACA‑CACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGa < 4:102198/135‑1 (MQ=255) aaTCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACA‑CACTGAATCCTGATCAATAAAAAAACTTCCa < 7:116392/93‑1 (MQ=255) aaTCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACA‑CACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTg > 5:200664/1‑138 (MQ=255) atccccGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACA‑CACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGa < 7:205697/135‑1 (MQ=255) cccGTTACCCGCGGCAATCTGACCGCGGTAATATTTATGATGGGCTGTTTGTA‑CACACCACTCAACCCTCACCAATAAAAACACTTCCACAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGAc < 4:196820/139‑1 (MQ=255) cGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACA‑CACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGc > 2:293216/1‑139 (MQ=255) cGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACA‑CACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGc < 3:348580/139‑1 (MQ=255) gTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACA‑CACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCgagag > 7:173651/1‑125 (MQ=255) gTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACA‑CACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCgagag < 8:173651/125‑1 (MQ=255) gTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACA‑CACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCt > 3:112729/1‑139 (MQ=255) gccAATCTGACCGGGGGAATAGTTATGATGGGCTGGATGTACACCA‑CACTGAATCCTCATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTC‑AAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAg < 6:92288/137‑1 (MQ=255) gCAATCTGCCCGCGGGAATAGTTATGAGGGGCTGGATGTACTACA‑CACTTAATCCTGATCAAAAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAg < 2:289414/139‑1 (MQ=255) gcgGGAATAGTTATGATGGGCTGGATGTACTACA‑CACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGc > 6:71358/1‑139 (MQ=255) tagttatgatgGGCTGGATGTACTACA‑CACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTc < 6:185268/134‑1 (MQ=255) atgatgGGCTGGATGTACTACA‑CACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGatt > 3:249855/1‑137 (MQ=255) tgatgGGCTGGATGTACTACA‑CACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGatt < 7:204182/138‑3 (MQ=255) tgatgGGCTGGATGTACTACA‑CACTGAACCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGatt < 5:119595/138‑3 (MQ=255) atgGGCTGGATGTACTACA‑CACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCg < 6:161323/139‑1 (MQ=255) atgGGCTGGATGTACTACA‑CACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCg > 2:251468/1‑139 (MQ=255) ggCTGGATGTACTACA‑CACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGc < 5:71358/138‑1 (MQ=255) gCTGGATGTACTACA‑CACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCa > 7:188658/1‑138 (MQ=255) cTGGATGTACTACC‑CACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCaaa > 4:158332/1‑139 (MQ=255) cTGGATGTACTACA‑CACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGCTTCCGGCaaa > 6:81331/1‑139 (MQ=255) actACA‑CACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGc > 1:92934/3‑128 (MQ=255) actACA‑CACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGc < 2:92934/126‑1 (MQ=255) actACA‑CACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGACATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCTGATTCCGGCAACTGTAAtt > 5:273795/3‑138 (MQ=255) | CACATTCGCCATCTCACTTTTGATCGAGCACCCTGATACAGTGACAGTAATGGGAGCAGTCAGAAACTTAATTCCCGTTACACTCGGCAACCTGACCGCGGGAATGGTCATGATGGGCTGGATGTATTACA‑CGCTCAATCCCGATAAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGACTCCGGCAAATGTAATT > CP006881/3723807‑3724069 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |