Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 2,801,996 G→A A327T (GCC→ACC)  U712_14440 → UPF0118 membrane protein ytvI

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,801,9960GA96.4% 159.6 / NA 57A327T (GCC→ACC) U712_14440UPF0118 membrane protein ytvI
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (21/33);  minor base C (2/0);  total (23/34)
Fisher's exact test for biased strand distribution p-value = 1.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TATCGATTACGGGCCAGCTGCCCCAAGCAATCGGAATTGGTATCCTGTACCTCGTCGTTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCGCCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGAAATATGGTCCTATATCACCG  >  CP006881/2801862‑2802119
                                                                                                                                      |                                                                                                                           
tATCGATTACGGGCCAGCTGCCCCAAGCAATCGGAATTGGTATCCTGTACCTCGTCGTTCGTTTTCAGCGGCAGCTGGCAGAGCCCAAGCACCGAAGTTAATCTATCGGGATCGATCCGCAGGCAACACGCAACAcccc                                                                                                                         >  4:48801/1‑138 (MQ=255)
 aTCGATTACGGGCCAGCTGCCCCAAGCAATCGGAATTGGTATCCTGTACCTCGTCGTTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTg                                                                                                                        >  5:129376/1‑139 (MQ=255)
      ttACGGGCCAGCTGCCCCAAGCAATCGGAATTGGTATCCTGTACCTCGTCGTTGTTATTCAGCGGCAGCTGACAGAGCCCAAGATTCTAAGTAAATCTATCGGGATCGATCCGCTGGCCACACTCCTCCCCCTGTTTGc                                                                                                                   >  1:92650/1‑139 (MQ=255)
      ttACGGGCCAGCTGCCCCAAGCAATCGGAATTGGTATCCTGTACCTCGTCGTTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGc                                                                                                                   <  4:204417/139‑1 (MQ=255)
                   gCCCCAAGCAATCGGAATTGGTATCCTGTACCTCGTCGTTCTTATTAAGCGGCAGCTGTCAGAGCCCAAGCTACTAAGTAAATCTATCGGGATCGCTCCGCTGGCAACACTCATCACCCTGTTTGCCGGCGTTAAatta                                                                                                      >  5:150644/1‑139 (MQ=255)
                        aaGCAATCGGAATTGGTATCCTGTACCTCGTCGTTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCgg                                                                                                 <  1:300795/139‑1 (MQ=255)
                        aaGCAATCGGAATTGGTATCCTGTACCTCGTCGTTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCgg                                                                                                 <  4:309946/139‑1 (MQ=255)
                        aaGCAATCGGAATTGGTATCCTGTACCTCGTCGTTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCgg                                                                                                 <  1:341255/139‑1 (MQ=255)
                         aGCAATCGGAATTGGTATCCTGTACCTCGTCGTGGTTTTTCCACGGGCGCGGTCAGGGCCCCAGAGAGTAAGTTAAACACGCGGGGTCCAGCCGCCGGCCACCCGCCACACCCGGGTTGCCGGCTCTTAattattggtg                                                                                                >  2:76708/1‑135 (MQ=255)
                                  aaTTGGTATCCTGTACCTCGTCGTTCTTCTTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGa                                                                                       <  2:165409/139‑1 (MQ=255)
                                      ggTATCCTGTACCTCGTCGTTCTTATTAAGCGGCCGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCCACACACCTCACCCAGTTTGCCGGCTTTAAATTTTTCGGGTTTTTTGGACTGa                                                                                   >  4:366204/1‑139 (MQ=255)
                                               tACCTCGTCGTTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGa                                                                                       <  7:228033/126‑1 (MQ=255)
                                               tACCTCGTCGTTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGa                                                                                       >  8:228033/1‑126 (MQ=255)
                                               tACCTCGTCGTTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCggg                                                                            <  6:129376/137‑1 (MQ=255)
                                               tACCTCGTCGTTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCggg                                                                            <  6:100941/137‑1 (MQ=255)
                                               tACCTCGTCGTTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCggg                                                                            <  4:155747/137‑1 (MQ=255)
                                               tACCTAGTCGTTCTTATTGAGAGGCAGCTGACAGAGCACAAGATACTAAGTAATTTTATCGGGATCGATCCGCTGGCAACTCTAATCAACATGTTTGCCGGCGTTAAATTAATCGGGGTTTTAGGTCTGATTGCGGGAc                                                                          <  5:216741/139‑1 (MQ=255)
                                                    cgtcgtTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCCACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCt                                                                     >  8:130222/1‑139 (MQ=255)
                                                    cgtcgtTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCgg                                                                                                 <  7:254907/111‑1 (MQ=255)
                                                    cgtcgtTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCgg                                                                                                 >  8:254907/1‑111 (MQ=255)
                                                      tcgtTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCGTTAAATTATTAGGGTTTTTAGGACTGATTGCGGGACCGGCTGt                                                                   >  6:142214/1‑139 (MQ=255)
                                                      tcgtTCTTATTAAGCGGCAGCTGACAGAGCCCAAGATACTAATTAAATATATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGt                                                                   >  6:96065/1‑139 (MQ=255)
                                                         ttCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATGGCTCCGCTGGCAACACTCAACACCCGGTTTGCCGGCTTTAAATTATTCGGGTGTTTTGGCCTGATTGCGGGACCGGCTGTACt                                                                >  3:354519/1‑139 (MQ=255)
                                                         gtCTTATTCAGCGGCAGCTGACAGAGCACAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACt                                                                <  1:146662/138‑1 (MQ=255)
                                                                    cGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCatcatc                                                      >  8:296562/1‑138 (MQ=255)
                                                                    cGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATcc                                                     >  6:120014/1‑139 (MQ=255)
                                                                         gCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCt                                                <  1:329941/139‑1 (MQ=255)
                                                                              cAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTtcat                                           <  3:366204/139‑1 (MQ=255)
                                                                              cAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTtcat                                           <  5:353845/139‑1 (MQ=255)
                                                                                   cccAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTa                                                                                                           >  5:76206/1‑70 (MQ=255)
                                                                                   cccAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTa                                                                                                           <  6:76206/70‑1 (MQ=255)
                                                                                   cccAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGttttt                                                                                           <  4:12252/86‑1 (MQ=255)
                                                                                   cccAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGttttt                                                                                           >  3:12252/1‑86 (MQ=255)
                                                                                             tAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCCCCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCAt                             >  6:366747/1‑138 (MQ=255)
                                                                                               aGTAAATCTATCGGGATCGATCCGCTGGCAACTCTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTaa                          <  3:61846/139‑1 (MQ=255)
                                                                                                   aaTCTATCGGGATCGTTCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAg                       <  3:284379/138‑1 (MQ=255)
                                                                                                   aaTCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAg                       <  4:95842/138‑1 (MQ=255)
                                                                                                   aaTCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAg                       <  5:120014/138‑1 (MQ=255)
                                                                                                     tCTATCGGGATCGATCCGCTGGCAACCCTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGACATCATCCAAGCTTTCATCACACCAGGCGCATTAAAAGaaa                    >  8:201768/1‑139 (MQ=255)
                                                                                                       tATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAg                       <  5:213931/134‑1 (MQ=255)
                                                                                                       tATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAg                       >  6:213931/1‑134 (MQ=255)
                                                                                                         tCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCAt                             <  4:104178/126‑1 (MQ=255)
                                                                                                         tCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGAAata                  <  5:96065/137‑1 (MQ=255)
                                                                                                         tCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGAAata                  <  7:296562/137‑1 (MQ=255)
                                                                                                         tCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGAAata                  <  6:150644/137‑1 (MQ=255)
                                                                                                         tCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGAAata                  <  3:48801/137‑1 (MQ=255)
                                                                                                         tCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGAAata                  <  5:142214/137‑1 (MQ=255)
                                                                                                         tCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGAAata                  <  4:354519/137‑1 (MQ=255)
                                                                                                         tCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGAAATATg                >  3:302143/1‑139 (MQ=255)
                                                                                                         tCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGAAATATg                >  4:148055/1‑139 (MQ=255)
                                                                                                         tCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTAAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCAt                             >  3:104178/1‑126 (MQ=255)
                                                                                                         tCGGGATCGATCCGCTGGAAACACTCCTCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGAAata                  <  4:324777/137‑1 (MQ=255)
                                                                                                           gggATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGAAATATgg               <  3:250733/138‑1 (MQ=255)
                                                                                                           gggATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGAAATATgg               <  5:129381/138‑1 (MQ=255)
                                                                                                               tcgatcCGCTGGCAACACTCATCTCCCTGTTTGGCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACAGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGAAATATGGTCCta          <  7:219889/139‑1 (MQ=255)
                                                                                                               tcgatcCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCAGTAAAAGAAATATGGTCCta          >  3:366423/1‑139 (MQ=255)
                                                                                                                       cTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGAAATATGGTCCTATATCACCg  <  3:148055/139‑1 (MQ=255)
                                                                                                                                      |                                                                                                                           
TATCGATTACGGGCCAGCTGCCCCAAGCAATCGGAATTGGTATCCTGTACCTCGTCGTTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCGCCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGAAATATGGTCCTATATCACCG  >  CP006881/2801862‑2802119

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑