Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 3,723,067 1 bp→AT intergenic (‑181/‑120) U712_19190 ← / → U712_19195 PTS system sucrose‑specific EIIBC component/putative transporter ywcJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068813,723,0661.A95.5% 296.6 / 6.8 88intergenic (‑180/‑121)U712_19190/U712_19195PTS system sucrose‑specific EIIBC component/putative transporter ywcJ
Reads supporting (aligned to +/- strand):  ref base . (1/3);  new base A (31/53);  total (32/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01
*CP0068813,723,0670GT95.5% 287.1 / 1.6 88intergenic (‑181/‑120)U712_19190/U712_19195PTS system sucrose‑specific EIIBC component/putative transporter ywcJ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base T (31/53);  minor base A (1/3);  total (32/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01

CGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTGAGAATACGCTTTCATCATAAATTATGATAGCGTTTTCGTCAACT‑GTTT‑TTTAGTTAAATAGTTTGATAAAACTTTTAGACAAAAATTCGTAAAAACTATTTGTGAAAAACTTCACAATATCGTGCTATACTATGCTCAACCATCAAAGAAAGCAGGAAAAAACAATGG  >  CP006881/3722935‑3723190
                                                                                                                                     ||                                                                                                                           
cGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACT‑Attt‑ttt                                                                                                                       <  7:55836/139‑1 (MQ=255)
        ggACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTT‑TTTAGTTaaa                                                                                                                <  5:115428/139‑1 (MQ=255)
        ggACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTT‑TTTAGTTaaa                                                                                                                <  5:15943/139‑1 (MQ=255)
        ggACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTT‑TTTAGTTaaa                                                                                                                <  6:249622/139‑1 (MQ=255)
        ggACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTT‑TTTAGTTaaa                                                                                                                <  8:35241/139‑1 (MQ=255)
           cacaGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTT‑TTTAGTTAAATAg                                                                                                             >  1:136178/1‑139 (MQ=255)
            acaGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTT‑TTTAGTTAAATAGt                                                                                                            <  2:129753/139‑1 (MQ=255)
            acaGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTT‑TTTAGTTAAATAGt                                                                                                            >  7:40620/1‑139 (MQ=255)
             caGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTT‑TTTAGTTAAATAGtt                                                                                                           >  5:280504/1‑139 (MQ=255)
              aGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTT‑TTTAGTTAAATAGttt                                                                                                          >  1:257320/1‑139 (MQ=255)
              aGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTT‑TTTAGTTAAATAGttt                                                                                                          >  3:11614/1‑139 (MQ=255)
              aGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTT‑TTTAGTTAAATAGttt                                                                                                          >  7:34314/1‑139 (MQ=255)
              aGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACAAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTT‑TTTAGTTAAATAGttt                                                                                                          >  1:371650/1‑139 (MQ=255)
                cGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTT‑TTTAGTTAAATAGTTTGa                                                                                                        >  8:312563/1‑139 (MQ=255)
                 gATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTT‑TTTAGTTAAATAGTTTGAt                                                                                                       <  2:371650/139‑1 (MQ=255)
                   tCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTT‑TTTAGTTAAATAGTTTGATat                                                                                                     <  5:351894/139‑2 (MQ=255)
                   tCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTT‑TTTAGTTAAATAGTTTGATa                                                                                                      >  5:224931/1‑138 (MQ=255)
                   tCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTT‑TTTAGTTAAATAGTTTGATa                                                                                                      >  5:76339/1‑138 (MQ=255)
                   tCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTT‑TTTAGTTAAATAGTTTGATa                                                                                                      >  3:371828/1‑138 (MQ=255)
                     cTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACT‑Attt‑t                                                                                                                         >  5:130262/1‑116 (MQ=255)
                     cTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACT‑Attt‑t                                                                                                                         <  6:130262/116‑1 (MQ=255)
                               tACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTACTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTT‑TTTAGTTAAATAGTTTGATATAACATGTAGAc                                                                                          >  8:178612/1‑138 (MQ=255)
                                  gCAAATTTTAGGTCTTGCCTGCTTTACAAGTCACAATCCCGCTTATTAAGATTAAGAATACGCTTTCATCATAAAACATGATAGCGTTTTCGTCAACTATTTT‑TTTAGTTAAATAGTTTTATATAAAATGTAGACaaa                                                                                       >  6:200207/1‑138 (MQ=255)
                                                  gCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTT‑TTTAGTTAAATAGTTTGATat                                                                                                     >  6:275200/1‑107 (MQ=255)
                                                  gCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTT‑TTTAGTTAAATAGTTTGATat                                                                                                     <  5:275200/108‑2 (MQ=255)
                                                  gCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTT‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTaaaaatt                                                                         <  5:376291/136‑3 (MQ=255)
                                                  gCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTT‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTaat                                                                      >  6:105380/1‑137 (MQ=255)
                                                  gCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTT‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTaat                                                                      >  1:127792/1‑137 (MQ=255)
                                                  gCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTT‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTaa                                                                       <  8:34314/138‑2 (MQ=255)
                                                  gCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTT‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTaa                                                                       <  3:168616/138‑2 (MQ=255)
                                                       cTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTT‑TTTAGTTTAATATTTTGATATAACATGTAGACAAAAATTAGTAAAAATTAATtgtg                                                                  >  2:231570/1‑138 (MQ=255)
                                                       cTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTT‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATtgtg                                                                  <  4:189309/138‑1 (MQ=255)
                                                          tACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTT‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaa                                                               >  1:320074/1‑138 (MQ=255)
                                                          tACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTT‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaa                                                               <  2:136178/138‑1 (MQ=255)
                                                          tACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTT‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaa                                                               <  2:127792/138‑1 (MQ=255)
                                                           aCCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTT‑TTTAGTTAAATAGTTTGATATAACATGTAGAAAAAAATTCGAAAAAATTAATTGTGaaat                                                              >  8:369589/1‑137 (MQ=255)
                                                            ccAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGAGTTTTCGTCAACTATTTT‑TTTAGTTAAATAGTTAGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaata                                                             <  3:95154/138‑3 (MQ=255)
                                                            ccAGTCACAATCCCGCTTATTCAGATTAAGAATAAGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTT‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaata                                                             <  8:44458/138‑3 (MQ=255)
                                                            ccAGTCACAATCCAGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACT‑ATTTATTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaata                                                             <  1:37530/138‑3 (MQ=255)
                                                              aGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTT‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACtt                                                          <  5:109840/139‑1 (MQ=255)
                                                                tCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATATCGTTTTCGTCAACTATTTT‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTca                                                        <  5:147074/139‑1 (MQ=255)
                                                                tCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTT‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTca                                                        <  6:153890/139‑1 (MQ=255)
                                                                tCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTT‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTca                                                        <  1:57047/139‑1 (MQ=255)
                                                                tCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTT‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTca                                                        <  6:159597/139‑1 (MQ=255)
                                                                  acaATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTT‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTcaca                                                      <  4:160328/139‑1 (MQ=255)
                                                                     aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTT‑TTTAGTTAAATAGTTTGATCTAACATGTAGACAAAAATTCGTAAAAATTTATTGTGAAATACTTCACAata                                                   <  4:44724/139‑1 (MQ=255)
                                                                     aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTT‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                   <  7:255546/139‑1 (MQ=255)
                                                                     aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTT‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                   <  3:367715/139‑1 (MQ=255)
                                                                     aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTT‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                   <  6:155076/139‑1 (MQ=255)
                                                                     aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTT‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                   <  6:280504/139‑1 (MQ=255)
                                                                     aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTT‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                   <  6:283151/139‑1 (MQ=255)
                                                                     aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTT‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                   <  6:224931/139‑1 (MQ=255)
                                                                     aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTT‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                   <  2:320074/139‑1 (MQ=255)
                                                                     aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTT‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                   <  8:249560/139‑1 (MQ=255)
                                                                     aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTT‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                   <  8:40620/139‑1 (MQ=255)
                                                                     aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTT‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                   <  1:168696/139‑1 (MQ=255)
                                                                     aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTT‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                   <  5:788/139‑1 (MQ=255)
                                                                     aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTT‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                   <  5:320254/139‑1 (MQ=255)
                                                                     aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTT‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                   <  4:27610/139‑1 (MQ=255)
                                                                     aTCCAGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTT‑TTTAGTTGAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                   <  8:366262/139‑1 (MQ=255)
                                                                        ccGCTTATTCAGATTAATAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTT‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGCGAAATACTTCACAAAATCg                                                <  4:329701/139‑1 (MQ=255)
                                                                        ccGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTT‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATc                                                 <  4:151825/138‑1 (MQ=255)
                                                                        ccGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTT‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATc                                                 <  4:371828/138‑1 (MQ=255)
                                                                        ccGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTT‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATc                                                 <  6:76339/138‑1 (MQ=255)
                                                                        ccGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTT‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATc                                                 <  4:11614/138‑1 (MQ=255)
                                                                        ccGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTT‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCg                                                <  5:105380/139‑1 (MQ=255)
                                                                          gcatATTCAGATTAAGAATACTCTTTCATCATAAATCATTATAGAGTTTTCGTCAACTATTTT‑TTTAGGTAAATAGCTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                                              <  1:258432/136‑1 (MQ=255)
                                                                          gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTT‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                                              <  3:251802/139‑1 (MQ=255)
                                                                          gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTT‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                                              <  2:219523/139‑1 (MQ=255)
                                                                              aTTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTT‑TTTTGTTAAATAGTTTGATATAACATGTAGCCAAAAATTCGAAAAAATTAATTGTGAAATCCTTCACAATATCGTGCta                                           >  2:57926/1‑138 (MQ=255)
                                                                               ttCAGATTAAGAATACTCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTT‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCtata                                         <  7:312563/139‑1 (MQ=255)
                                                                                tCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTT‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCtata                                         >  4:176337/1‑138 (MQ=255)
                                                                                  aGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTT‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACt                                       <  4:151344/138‑1 (MQ=255)
                                                                                    aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTT‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                                              <  7:207816/129‑1 (MQ=255)
                                                                                    aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTT‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                                              >  8:207816/1‑129 (MQ=255)
                                                                                            tACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTT‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCa                            <  7:52854/139‑1 (MQ=255)
                                                                                              cGCTTTCATCATAAATCATGATAGGGTTTTCGTCAACTATTTT‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAATTACTTCACAATATCGTGCTATACTATGCTCAATCAt                           >  4:184720/1‑138 (MQ=255)
                                                                                              cGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTT‑TTTAGTTAATTAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTATTTGTGAATTACTTCACATTATCGTGCTATACTATGCTCAATCAtg                          >  6:354680/1‑138 (MQ=255)
                                                                                              cGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTT‑TTTAGTTAAATAGTTTGATATAAAATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATAATTCACAATATCGTGGTATAGTATGCTCAATCAt                           >  2:298750/1‑138 (MQ=255)
                                                                                                    catcatAAATCATGATAGCGTTTTCGTCAACTATTTT‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGaaaga                     >  5:134262/1‑138 (MQ=255)
                                                                                                         tAAATCATGATAGCGTTTTCGTCAACTATTTT‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAg               >  4:80934/1‑139 (MQ=255)
                                                                                                           aaTCATGATAGCGTTTTCGTCAACTATTTT‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGa             <  7:75587/139‑1 (MQ=255)
                                                                                                               aTGATAGCGTTTTCGTCAACTATTTT‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGaa            >  2:69484/1‑136 (MQ=255)
                                                                                                                      ctttttCGTCAACTATTTT‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTTAAATACTTCACAATATCGTGCTATACTTTGCTCAATCATGAAAGAAATCAGGAAAAGACAATg   <  5:200207/136‑1 (MQ=255)
                                                                                                                      cGTTTTCGTCAACTATTTT‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                   <  4:349278/90‑1 (MQ=255)
                                                                                                                      cGTTTTCGTCAACTATTTT‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                   >  3:349278/1‑90 (MQ=255)
                                                                                                                      cGTTTTCGTCAACTATTTT‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATg   >  3:201452/1‑138 (MQ=255)
                                                                                                                       gTTTTCGTCAACTATTTT‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATgg  >  6:203703/1‑138 (MQ=255)
                                                                                                                                     ||                                                                                                                           
CGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTGAGAATACGCTTTCATCATAAATTATGATAGCGTTTTCGTCAACT‑GTTT‑TTTAGTTAAATAGTTTGATAAAACTTTTAGACAAAAATTCGTAAAAACTATTTGTGAAAAACTTCACAATATCGTGCTATACTATGCTCAACCATCAAAGAAAGCAGGAAAAAACAATGG  >  CP006881/3722935‑3723190

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑