Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 832,829 C→A 8.2% G79G (GGC→GGA)  U712_04045 → putative polysaccharide deacetylase pdaA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP006881832,8290CA 8.2% 158.1 / 3.1 49G79G (GGC→GGA) U712_04045putative polysaccharide deacetylase pdaA
Reads supporting (aligned to +/- strand):  ref base C (19/26);  new base A (2/2);  total (21/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

TAAACGAAGTGTCAATCATCAGCCGCCAGATGCCGGGAAGCAGCTGAACAGTTTAATTGAAAAATATGACGCGTTTTATTTAGGGAATACAAAGGAAAAAACGATTTACTTAACGTTTGATAACGGATATGAAAATGGCTATACGCCAAAAGTGCTTGATGTCTTAAAAAAACATCGCGTAACAGGAACGTTTTTTGTCACCGGGCATTTCGTAAAGGACCAGCCTCAGCTGATCAAACGAATGTCAGATGAGGGCCATATTATCGGCAATCATTCC  >  CP006881/832691‑832967
                                                                                                                                          |                                                                                                                                          
tAAACGAAGTGTCAATCATCAGCCGCCAGATGCCGGGAAGCAGCTGAACAGTTTAATTGAAAAATATGACGCGTTTTATTTAGGGAATACAAAGGAAAAAACGATTTACTTAACGTTTGATAACGGATATGAAAATGGc                                                                                                                                            >  5:255979/1‑139 (MQ=255)
  aaCGAAGTGTCAATCATCAGCCGCCAGATGCCGGGAAGCAGCTGAACAGTTTAATTGAAAAATATGACGCGTTTTATTTAGGGAATACAAAGGAAAAAACGATTTACTTAACGTTTGATAACGGATATGAAAATGGCta                                                                                                                                          <  1:64856/139‑1 (MQ=255)
  aaCGAAGTGTCAATCATCAGCCGCCAGATGCCGGGAAGCAGCTGAACAGTTTAATTGAAAAATATGACGCGTTTTATTTAGGGAATACAAAGGAAAAAACGATTTACTTAACGTTTGATAACGGATATGAAAATGGCta                                                                                                                                          <  5:243875/139‑1 (MQ=255)
  aaCGAAGTGTCAATCATCAGCCGCCAGATGCCGGGAAGCAGCTGAACAGTTTAATTGAAAAATATGACGCGTTTTATTTAGGGAATACAAAGGAAAAAACGATTTACTTAACGTTTGATAACGGATATGAAAATGGCta                                                                                                                                          <  2:94618/139‑1 (MQ=255)
                         ccAGATGCCGGGAAGCAGCTGAACAGTTTAATTGAAAAATATGACGCGTTTTATTTAGGGAATACAAAGGAAAAAACGATTTACTTAACGTTTGATAACGGATATGAAAATGGCTATACGCCAAAAGTGCTTGATGTCt                                                                                                                   >  3:161170/1‑139 (MQ=255)
                         ccAGATGCCGGGAAGCAGCTGAACAGTTTAATTGAAAAATATGACGCGTTTTATTTAGGGAATACAAAGGAAAAAACGATTTACTTAACGTTTGATAACGGATATGAAAATGGCTATACGCCAAAAGTGCTTGATGTCt                                                                                                                   >  4:169940/1‑139 (MQ=255)
                              tGCCGGGAAGCAGCTGAACAGTTTAATTGAAAAATATGACGCGTTTTATTTAGGGAATACAAAGGAAAAAACGATTTACTTAACGTTTGATAACGGATATGAAAATGGCTATACGCCAAAAGTGCTTGATGTCTTaaaa                                                                                                              <  1:121880/139‑1 (MQ=255)
                                   ggAAGCAGCTGAACAGTTTAATTGAAAAATATGACGCGTTTTATTTAGGGAATACAAAGGAAAAAACGATTTACTTAACGTTTGATAACGGATATGAAAATGGCTATACGCCAAAAGTGCTTGATGTCTTAAAAAAACa                                                                                                         <  6:145895/139‑1 (MQ=255)
                                   ggAAGCAGCTGAACAGTTTAATTGAAAAATATGACGCGGGTTATTTAGGGACTACAAAGGAAAAAACGCTTTACTTAACGTTTGATAACGGATATGAAAATGGCTATACGCCAAAAGTGCTTGATGGCTTAAAAAAACa                                                                                                         <  6:145897/139‑1 (MQ=255)
                                                   tttAATTGAAAAATATGACGCGTTTTATTTAGGGAATACAAATGAAAAAACGATTTACTTAACGTTTGAGAACGGATATGAAAATGGATATACGCCAAAAGTGCTTGATCTCTTAAAAAAACATCGCTTAACAGGAACg                                                                                         >  1:64942/1‑139 (MQ=255)
                                                   tttAATTGAAAAATATGACGCGTTTTATTTAGGGAATACAAAGGAAAAAACGATTTACTTAACGTTTGATAACGGATATGAAAATGGCTATACGCCAAAAGTGCTTGATGTCTTAAAAAAACATCGCGTAACAGGACCg                                                                                         >  8:256833/1‑139 (MQ=255)
                                                   tttAATTGAAAAATATGACGCGTTTTATTTAGGGAATACAAAGGAAAAAACGATTTACTTAACGTTTGATAACGGATATGAAAATGGCTATACGCCAAAAGTGCTTGATGTCTTAAAAAAACATCGCGTAACAGGAACg                                                                                         >  6:162255/1‑139 (MQ=255)
                                                   tttAATTGAAAAATATGACGCGTTTTATTTAGGGAATACAAAGGAAAAAACGATTTACTTAACGTTTGATAACGGATATGAAAATGGATATACGCCAAAAGTGCTTGATGTCTTAAAAAAAAATCGCGTAACAGGAACg                                                                                         >  1:69409/1‑139 (MQ=255)
                                                           aaaaaTATGACGCGTTTTATTTAGGGAATACAAAGCAAAAAACGATTTACTTAACGATTGATAACGGATATGAAAATGGCTATACGCCAAAAGTGCTTGATGTCTTAAAAAAACATCGCGTAACAGGAACGTTTTTTGt                                                                                 >  3:25619/1‑139 (MQ=255)
                                                             aaaTATGACGCGTTTTATTTAGGGAATACAAAGGAAAAAACGATTTACTTAACGTTTGATAACGGATATGAAAATGGCTATACGCCAAAAGTGCTTGATGTCTTAAAAAAACATCGCGTAACAGGAACGTTTTTTGTCa                                                                               >  1:243193/1‑139 (MQ=255)
                                                             aaaTATGACGCGTTTTATTTAGGGAATACAAAGGAAAAAACGATTTACTTAACGTTTGATAACGGATATGAAAATGGCTATACGCCAAAAGTGCTTGATGTCTTAAAAAAACATCGCGTAACAGGAACGTTTTTTGTCa                                                                               >  2:285384/1‑139 (MQ=255)
                                                                          tttatttaGGGAATACAAAGGAAAAAACGATTTACTTAACGTTTGATAACGGATATGAAAATGGCTATACGCCAAAAGTGCTTGATGTCTTAAAAAAACATCGCGTAACAGGAACGTTTTTTGTCACCGGGCATTTCg                                                                   >  2:187038/1‑138 (MQ=255)
                                                                          tttatttaGGGAATACAAAGGAAAAAACGATTTACTTAACGTTTGATAACGGATATGAAAATGGCTATACGCCAAAAGTGCTTGATGTCTTAAAAAAACATCGCGTAACAGGAACGTTTTTTGTCACCGGGCATTTCg                                                                   >  4:63067/1‑138 (MQ=255)
                                                                          tttatttaGGGAATACAAAGGAAAAAACGATTTACTTAACGTTTGATAACGGATATGAAAATGGCTATACGCCAAAAGTGCTTGATGTCTTAAAAAAACATCGCGTAACAGGAACGTTTTTTGTCACCGGGCATTTCg                                                                   >  4:194109/1‑138 (MQ=255)
                                                                          tttatttaGGGAATACAAAGGAAAAAACGATTTACTTAACGTTTGATAACGGATATGAAAATGGCTATACGCCAAAAGTGCTTGATGTCTTAAAAAAACATCGCGTAACAGGAACGTTTTTTGTCACCGGGCATTTCg                                                                   >  6:253448/1‑138 (MQ=255)
                                                                            tatttaGGGAATACAAAGGAAAAAACGATTTACTTAACGTTTGATAACGGATATGAAAATGGCTATACGCCAAAAGTGCTTGATGTCTTAAAAAAACATCGCGTAACAGGAACGTTTTTTGTCACCGGGCATTTCGTa                                                                 <  3:194109/138‑1 (MQ=255)
                                                                                taGGGAATACAAAGGAAAAAACGATTTACTTAACGTTTGATAACGGATATGAAAATGGCTATACGCCAAAAGTGCTTGATGTCTTAAAAAAACATCGCGTAACAGGAACGTTTTTTGTCACCGGGCATTTCGTAAAgg                                                             <  8:50062/138‑1 (MQ=255)
                                                                                taGGGAATACAAAGGAAAAAACGATTTACTTAACGTTTGATAACGGATATGAAAATGGCTATACGCCAAAAGTGCTTGATGTCTTAAAAAAACATCGCGTAACAGGAACGTTTTTTGTCACCGGGCATTTCGTAAAgg                                                             <  8:45388/138‑1 (MQ=255)
                                                                                 atggAATAGAAAGGAAAAAACGATTTACTTAACGTTTGATAACGGATATGAAAATGGCTTTACGCCAAAAGTGCTTGATGTCTTAAAAAAACATCGCGTAACAGGATCGTTTTTTGTCACCGGGCATTTCGTAAAGGa                                                            <  4:223655/136‑1 (MQ=255)
                                                                                 atggAATACAAAGGAAATAACTCTTTACTTAACGTTTGATAACGGATATGAAAATGGATATACGCCAAAAGTGCTTGATGTCTTAAAAAATCATCGCGTAACAGGAACGTTTTTTGTCACCGGGCATTTCGTAAAGGa                                                            <  2:64942/136‑1 (MQ=255)
                                                                                 atggAATACAAAGGAAAAAACTATTTACTTAACGGTTGATAACGGATATGAAAATTGATATACGCCAAAAGTGCTTGATGTCTTAAAAAAACATCTCGTAACAGGAACGTTTTTTGTCACCGGGCATTTCGTAAAGGa                                                            <  2:69409/136‑1 (MQ=255)
                                                                                 aGGGAATACAAAGGAAAAAACGATTTACTTAACGTTTGATAACGGATATGAAAATGGCTATACGCCAAAAGTGCTTGATGTCTTAAAAAAACATCGCGTAACAGGAACGTTTTTTGTCACCGGGCATTTCGTAAAGGa                                                            <  4:161170/138‑1 (MQ=255)
                                                                                 aGGGAATACAAAGGAAAAAACGATTTACTTAACGTTTGATAACGGATATGAAAATGGCTATACGCCAAAAGTGCTTGATGTCTTAAAAAAACATCGCGTAACAGGAACGTTTTTTGTCACCGGGCATTTCGTAAAGGa                                                            <  6:255979/138‑1 (MQ=255)
                                                                                 aGGGAATACAAAGGAAAAAACGATTTACTTAACGTTTGATAACGGATATGAAAATGGCTATACGCCAAAAGTGCTTGATGTCTTAAAAAAACATCGCGTAACAGGAACGTTTTTTGTCACCGGGCATTTCGTAAAGGa                                                            <  8:2032/138‑1 (MQ=255)
                                                                                 aGGGAATACAAAGGAAAAAACGATTTACTTAACGTTTGATAACGGATATGAAAATGGCTATACGCCAAAAGTGCTTGATGTCTTAAAAAAACATCGCGTAACAGGAACGTTTTTTGTCACCGGGCATTTCGTAAAGGa                                                            <  5:44101/138‑1 (MQ=255)
                                                                                  gggAATACAAAGGAAAAAACGATTTACTTAACGTTTGATAACGGATATGAAAATGGCTATACGCCAAAAGTGCTTGATGTCTTAAAAAAACa                                                                                                         <  4:55190/92‑1 (MQ=255)
                                                                                  gggAATACAAAGGAAAAAACGATTTACTTAACGTTTGATAACGGATATGAAAATGGCTATACGCCAAAAGTGCTTGATGTCTTAAAAAAACa                                                                                                         >  3:55190/1‑92 (MQ=255)
                                                                                   ggAATACAAAGGAAAAAACGATTTACTTAACGTTTGATAACGGATATGAAAATGGCTATACGCCAAAAGTGCTTGATGTCTTAAAAAAACATCGCGTAACAGGAACGTTTTTTGTCACCGGGCATTTCGTAAAGGa                                                            <  8:279065/136‑1 (MQ=255)
                                                                                   ggAATACAAAGGAAAAAACGATTTACTTAACGTTTGATAACGGATATGAAAATGGCTATACGCCAAAAGTGCTTGATGTCTTAAAAAAACATCGCGTAACAGGAACGTTTTTTGTCACCGGGCATTTCGTAAAGGa                                                            >  7:279065/1‑136 (MQ=255)
                                                                                       tACAAAGGAAAAAACGATTTACTTAACGTTTGATAACGGATATGAAAATGGCTATACGCCAAAAGTGCTTGATGTCTTAAAAAAACATCGCGTAACAgg                                                                                             >  4:238451/1‑99 (MQ=255)
                                                                                       tACAAAGGAAAAAACGATTTACTTAACGTTTGATAACGGATATGAAAATGGCTATACGCCAAAAGTGCTTGATGTCTTAAAAAAACATCGCGTAACAgg                                                                                             <  3:238451/99‑1 (MQ=255)
                                                                                          aaaGGAAAAAACGATATACTTAACGTTTGATAACGGATATGAAAATGGCTATACGCCAAAAGTGCTTGATGTCTTAAAAAAACATCGCGTAACAGGAACGTTTTTTGTCTCCGGGCATTTCGTAAAGGACCAGCCTCAg                                                  <  1:53250/139‑1 (MQ=255)
                                                                                               aaaaaaCGATTTACTTAACGTTTGATAACGGATATGAAAATGGCTATACGCCAAAAGTGCTTGATGTCTTAAAAAAACATCGCGTAACAGGAACGTTTTTTGTCACCGGGCATTTCGTAAAGGACCAGCCTCAGCTGAt                                             >  4:300747/1‑139 (MQ=255)
                                                                                                 aaaaCGATTTACTTAACGTTTGATAACGGATATGAAAATGGCTATACGCCAAAAGTGCTTGATGTCTTAAAAAAACATCGCGTAACAGGAACGTTTTTTGTCACCGGGCATTTCGTAAAGGACCAGCCTCAGCTGATc                                            >  3:257302/1‑138 (MQ=255)
                                                                                                     cGATTTACTTAACGTTTGATAACGGATATGAAAATGGCTATACGCCAAAAGTGCTTGATGTCTTAAAAAAACATCGCGTAACAGGAACGTTTTTTGTCACCGGGCATTTCGTAAAGGACCAGCCTCAGCTGATCAAACg                                       <  6:116886/139‑1 (MQ=255)
                                                                                                     cGATTTACTTAACGTTTGATAACGGATATGAAAATGGCTATACGCCAAAAGTGCTTGATGTCTTAAAAAAACATCGCGTAACAGGAACGTTTTTTGTCACCGGGCATTTCGTAAAGGACCAGCCTCAGCTGATCAAACg                                       <  7:256833/139‑1 (MQ=255)
                                                                                                        tttACTTAACGTTTGATAACGGATATGAAAATGGCTATACGCCAAAAGTGCTTGATGTCTTAAAAAAACATCGCGTAACAGGAACGTTTTTTGTCACCGGGCATTTCGTAAAGGACCAGCCTCAGCTGATCAAACGAAt                                    >  3:181951/1‑139 (MQ=255)
                                                                                                                  gcttGATAACGGATATGAAAATGGCAATACGCCAAAAGTGCTTGATGTCTTAAAAAAACAACGCGTAACAGGAACGTTTTTTGTCACCGGGCATTTCGTAAAGGACCAGCCTCAGCTGATCAAACGAAAGTCAGATGAg                          <  6:267242/137‑1 (MQ=255)
                                                                                                                     tGATAACGGATATGAAAATGGCTATACGCCAAAAGTGCTTGATGTCTTAAAAAAACATCGCGTAACAGGAACGTTTTTTGTCACCGGGCATTTCGTAAAGGACCAGCCTCAGCTGATCAAACGAATGTCAGATGAggg                        <  4:116290/138‑1 (MQ=255)
                                                                                                                      gATAACGGATATGAAAATGGCTATACGCCAAAAGTGCTTGATGTCTTAAAAAAACATCGCGTAACAGGAACGTTTTTTGTCACCGGGCATTTCGTAAAGGACCAGCCTCAGCTGATCAAACGAATGTCAGATGAGGGc                       >  3:307128/1‑138 (MQ=255)
                                                                                                                              atatGAAAATGGCTATACGCCAAAAGTGCTTGATGTCTTAAAAAAACATCGCGTAACAGGAACGTTTTTTGTCACCGGGCATTTCGTAAAGGACCAGCCTCAGCTGATCAAACGAATGTCAGATGAGGGCCATATTATc              <  4:307128/139‑1 (MQ=255)
                                                                                                                              agatGAAAATGGCTATACGCCAAAAGTGCTTGATGTCTTAAAAACACCTCGCGAAACAGGCACGTTTTTTGTCACCGGCCATTGCGCACAGGACCAGCCTCAGCTGATCAAACGAATGGCAGATGAGGGCCATATTATc              <  5:270670/137‑1 (MQ=255)
                                                                                                                                     aaTGGCTATACGCCAAAAGTGCTTGATGTCTTAAAAAAACATCGCGTAACAGGAACGTTTTTTGTCACCGGGCATTTCGTAAAGGa                                                            >  7:89361/1‑86 (MQ=255)
                                                                                                                                     aaTGGCTATACGCCAAAAGTGCTTGATGTCTTAAAAAAACATCGCGTAACAGGAACGTTTTTTGTCACCGGGCATTTCGTAAAGGa                                                            <  8:89361/86‑1 (MQ=255)
                                                                                                                                     aaTGGCTATACGCCAAAAGTGCTTGATGTCTTAAAAAAACATCGCGTAACAGGAACGTTTTTTGTCACCGGGCATTTCGTAAAGGACCAGCCTCAGCTGATCAAACGAATGTCAGATGAGGGCCATATTATCGGCAATc       <  3:300747/139‑1 (MQ=255)
                                                                                                                                          cTATACGCCAAAAGTGCTTGATGTCTTAAAAAAACATCGCGTAACAGGAACGTTTATTGTCACCGGGCATTTCGTAAAGGACCAGCCTCGGCTGATCAAAAGAATGTCAGATGAGGGCCATATTATCTGCAATCATTcc  >  7:206886/1‑139 (MQ=255)
                                                                                                                                          |                                                                                                                                          
TAAACGAAGTGTCAATCATCAGCCGCCAGATGCCGGGAAGCAGCTGAACAGTTTAATTGAAAAATATGACGCGTTTTATTTAGGGAATACAAAGGAAAAAACGATTTACTTAACGTTTGATAACGGATATGAAAATGGCTATACGCCAAAAGTGCTTGATGTCTTAAAAAAACATCGCGTAACAGGAACGTTTTTTGTCACCGGGCATTTCGTAAAGGACCAGCCTCAGCTGATCAAACGAATGTCAGATGAGGGCCATATTATCGGCAATCATTCC  >  CP006881/832691‑832967

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑