Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 3,723,652 C→T 100% L156L (CTG→TTG)  U712_19195 → putative transporter ywcJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068813,723,6520CT100.0% 166.4 / NA 51L156L (CTG→TTG) U712_19195putative transporter ywcJ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/28);  total (23/28)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GCCGGCAATTTGATCGGCGCCATTCTATTTGCCATTCTGATCAGCGCGACAGGGCTTTTTGAGGAGGCTTCTGTTCATTCCTTTTTGATTCATCTGGCAGAGCACAAAATGGAGCCGCCCGCTTCCGAACTGTTTTTCAGAGGGATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTTAAAGGGGAAGGGGCAAAGCTCTTTACCATGATGCTTTTCGTTTTCTGCTTCTTCATTTCCGGCTTCGAAC  >  CP006881/3723523‑3723787
                                                                                                                                 |                                                                                                                                       
gCCGGCAATTTAATCGGCGCCATTCTGTTTCCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCa                                                                                                                                >  6:56720/1‑139 (MQ=255)
   ggCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCagag                                                                                                                             <  5:22153/139‑1 (MQ=255)
     cAATTTAATCGGGGCCATTATGTTTCCAAACCAGAAGAGCGCGACGGGAGTTTTTGAGGAGCCTTCTGTTAATACATTTTTGATTAATTTGGAAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAgga                                                                                                                           <  5:198647/139‑2 (MQ=255)
     cAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAgg                                                                                                                            >  3:21089/1‑138 (MQ=255)
     aaaTTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAgga                                                                                                                           <  5:57078/138‑2 (MQ=255)
         taatggggCGCAATTTTTTGTGCCATCCTTTGCAGCGCGACGGGACTTTTTGTGTCGCCTTCTGTTCATTCCTTTTTTATTCATTTGGCACAGAACAAAATGTAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATg                                                                                                                        <  6:203172/132‑1 (MQ=255)
            aTCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCtg                                                                                                                     <  2:66467/138‑1 (MQ=255)
               ggCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCa                                                                                                                 <  1:44198/139‑1 (MQ=255)
                gcgcCATTCTGTTTGCCTTCCTCATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCaa                                                                                                                <  7:278895/139‑1 (MQ=255)
                  gcCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAAtt                                                                                                              >  3:236445/1‑139 (MQ=255)
                           tttGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTGCATTTTGATTAATTTGGCAGAGCACAAAATGGAGCCGCCCGCGTCCCAATTTTTTTTCATAGGAATGCTGTGCAATTTGGTtgttg                                                                                                     >  6:40570/1‑137 (MQ=255)
                                       aTCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTTATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCtttt                                                                                         >  6:240135/1‑139 (MQ=255)
                                       aTCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATg                                                                                                                        >  8:77204/1‑108 (MQ=255)
                                       aTCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATg                                                                                                                        <  7:77204/108‑1 (MQ=255)
                                        tCACCGCGAGGGGACTTTTTGAGGAGCCTTCTTTTCATTCCTTTTTGATTCTTTTGGCATACCACATAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCtttt                                                                                         <  1:80238/138‑1 (MQ=255)
                                            cgcgACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTTATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCAtt                                                                                    >  3:197089/1‑139 (MQ=255)
                                            cgcgACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCagag                                                                                                                             >  6:11601/1‑98 (MQ=255)
                                            cgcgACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCagag                                                                                                                             <  5:11601/98‑1 (MQ=255)
                                            cgcgACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCAtt                                                                                    >  1:97596/1‑139 (MQ=255)
                                                 cggtacTTTTTGAGGAGCTTTATGTTCATTCCTTTTTGATTCATTTGCCCCACCAAAAACTGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAAt                                                                               <  3:186365/134‑1 (MQ=255)
                                                   ggacTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGt                                                                             <  2:217360/136‑1 (MQ=255)
                                                   ggacTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGt                                                                             <  2:97596/136‑1 (MQ=255)
                                                            taggagcATTCTTTTAATTCCTTTTTGATTCATTTGTCAGCGAACAAAATGTAGCCGCCGGCTTCCGATTTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCaaa                                                                     <  3:266953/137‑1 (MQ=255)
                                                            gaggagCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCCATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGGCTCTAAaag                                                                    >  4:66762/1‑139 (MQ=255)
                                                                agccTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAaagggga                                                                <  7:58269/136‑1 (MQ=255)
                                                                agccTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAaagggga                                                                <  5:281532/136‑1 (MQ=255)
                                                                agccTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCAAAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAaagggga                                                                <  4:21089/136‑1 (MQ=255)
                                                                         ttCATTCCTTTTTGATTCATTTGGCAGAGCACAAAAGGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCa                                                         <  5:27021/137‑1 (MQ=255)
                                                                            aTTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGct                                                    >  4:117379/1‑139 (MQ=255)
                                                                              tCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATTCTGTGAAATTGGCTTTTGTGCCTCGCCTTTTTCATTACAATGTATCTCAAAGGAGAAGTAGCAAATcttt                                                  >  3:171428/1‑137 (MQ=255)
                                                                              tCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCttttt                                                                                        <  5:141862/101‑1 (MQ=255)
                                                                              tCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCttttt                                                                                        >  6:141862/1‑101 (MQ=255)
                                                                              tCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCAGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAAAGTGTCTAAAAGGGGAATGAGCAAAGcttt                                                  >  3:6397/1‑137 (MQ=255)
                                                                              tCCTTTTTGATACATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGCGGAAGGAGCAAAGcttt                                                  >  5:138729/1‑137 (MQ=255)
                                                                               ccTTTTTGATTCATTTCGCAGAGCAAAAAATGGAGCCGCCGGCTTCCGATTTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGcttt                                                  <  5:56720/138‑3 (MQ=255)
                                                                                cTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCtttt                                                 <  5:138770/138‑1 (MQ=255)
                                                                                          cATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCCAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGc                                      >  2:52067/1‑139 (MQ=255)
                                                                                                  agagCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGtt                              <  8:35356/139‑1 (MQ=255)
                                                                                                     gCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTc                           >  1:155150/1‑139 (MQ=255)
                                                                                                      cacaAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATTCTGTGCAATTGGCTTGTGTGCCTCGCC‑TTTTCATTCCAATGTCTCAAAAAGGGGAAGGCGCAAAGCTTTTTAACATGATGGTTTTGGTTTtgtg                         >  2:308161/1‑139 (MQ=255)
                                                                                                      cacaAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTATATCAAAGGGGAATTACCAAAGCTTTTTAACATGATTCTTTTGTttttgt                          >  6:168783/1‑137 (MQ=255)
                                                                                                            agggAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGcttct                    <  4:58851/137‑1 (MQ=255)
                                                                                                            aTGGAGCCGCCTGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTTACTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGcttct                    <  5:168783/139‑1 (MQ=255)
                                                                                                                aGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTAt                >  7:190928/1‑139 (MQ=255)
                                                                                                                  ccgccggcTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATTTATATCAAAGGGGAAGGAGCAAATCTTTTTACCATGATGCTTTTCTTTTTCGGCTGCTTTAttt              >  6:37810/1‑139 (MQ=255)
                                                                                                                   cgccggcTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTc             <  5:248535/139‑1 (MQ=255)
                                                                                                                   cgccggcTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTc             <  7:175566/139‑1 (MQ=255)
                                                                                                                     ccggcTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCg           >  3:214496/4‑139 (MQ=255)
                                                                                                                     ccggcTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTATTCATTCCAATGTCTCTCAAAGGGGAAGGAGATAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCGTTATTTACg           >  3:90328/4‑139 (MQ=255)
                                                                                                                        gcTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCt        <  6:260158/139‑1 (MQ=255)
                                                                                                                              gAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAac  <  8:190928/139‑1 (MQ=255)
                                                                                                                                 |                                                                                                                                       
GCCGGCAATTTGATCGGCGCCATTCTATTTGCCATTCTGATCAGCGCGACAGGGCTTTTTGAGGAGGCTTCTGTTCATTCCTTTTTGATTCATCTGGCAGAGCACAAAATGGAGCCGCCCGCTTCCGAACTGTTTTTCAGAGGGATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTTAAAGGGGAAGGGGCAAAGCTCTTTACCATGATGCTTTTCGTTTTCTGCTTCTTCATTTCCGGCTTCGAAC  >  CP006881/3723523‑3723787

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑