Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 3,723,666 G→A 100% G160G (GGG→GGA)  U712_19195 → putative transporter ywcJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068813,723,6660GA100.0% 155.0 / NA 47G160G (GGG→GGA) U712_19195putative transporter ywcJ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/26);  total (21/26)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TTGATCGGCGCCATTCTATTTGCCATTCTGATCAGCGCGACAGGGCTTTTTGAGGAGGCTTCTGTTCATTCCTTTTTGATTCATCTGGCAGAGCACAAAATGGAGCCGCCCGCTTCCGAACTGTTTTTCAGAGGGATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTTAAAGGGGAAGGGGCAAAGCTCTTTACCATGATGCTTTTCGTTTTCTGCTTCTTCATTTCCGGCTTCGAACACAGCATTGCC  >  CP006881/3723532‑3723798
                                                                                                                                      |                                                                                                                                    
taatggggCGCAATTTTTTGTGCCATCCTTTGCAGCGCGACGGGACTTTTTGTGTCGCCTTCTGTTCATTCCTTTTTTATTCATTTGGCACAGAACAAAATGTAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATg                                                                                                                                   <  6:203172/132‑1 (MQ=255)
   aTCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCtg                                                                                                                                <  2:66467/138‑1 (MQ=255)
      ggCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCa                                                                                                                            <  1:44198/139‑1 (MQ=255)
       gcgcCATTCTGTTTGCCTTCCTCATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCaa                                                                                                                           <  7:278895/139‑1 (MQ=255)
         gcCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAAtt                                                                                                                         >  3:236445/1‑139 (MQ=255)
                  tttGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTGCATTTTGATTAATTTGGCAGAGCACAAAATGGAGCCGCCCGCGTCCCAATTTTTTTTCATAGGAATGCTGTGCAATTTGGTtgttg                                                                                                                >  6:40570/1‑137 (MQ=255)
                              aTCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTTATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCtttt                                                                                                    >  6:240135/1‑139 (MQ=255)
                              aTCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATg                                                                                                                                   >  8:77204/1‑108 (MQ=255)
                              aTCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATg                                                                                                                                   <  7:77204/108‑1 (MQ=255)
                               tCACCGCGAGGGGACTTTTTGAGGAGCCTTCTTTTCATTCCTTTTTGATTCTTTTGGCATACCACATAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCtttt                                                                                                    <  1:80238/138‑1 (MQ=255)
                                   cgcgACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTTATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCAtt                                                                                               >  3:197089/1‑139 (MQ=255)
                                   cgcgACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCAtt                                                                                               >  1:97596/1‑139 (MQ=255)
                                        cggtacTTTTTGAGGAGCTTTATGTTCATTCCTTTTTGATTCATTTGCCCCACCAAAAACTGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAAt                                                                                          <  3:186365/134‑1 (MQ=255)
                                          ggacTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGt                                                                                        <  2:217360/136‑1 (MQ=255)
                                          ggacTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGt                                                                                        <  2:97596/136‑1 (MQ=255)
                                                   taggagcATTCTTTTAATTCCTTTTTGATTCATTTGTCAGCGAACAAAATGTAGCCGCCGGCTTCCGATTTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCaaa                                                                                <  3:266953/137‑1 (MQ=255)
                                                   gaggagCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCCATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGGCTCTAAaag                                                                               >  4:66762/1‑139 (MQ=255)
                                                       agccTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAaagggga                                                                           <  7:58269/136‑1 (MQ=255)
                                                       agccTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAaagggga                                                                           <  5:281532/136‑1 (MQ=255)
                                                       agccTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCAAAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAaagggga                                                                           <  4:21089/136‑1 (MQ=255)
                                                                ttCATTCCTTTTTGATTCATTTGGCAGAGCACAAAAGGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCa                                                                    <  5:27021/137‑1 (MQ=255)
                                                                   aTTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGct                                                               >  4:117379/1‑139 (MQ=255)
                                                                     tCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATTCTGTGAAATTGGCTTTTGTGCCTCGCCTTTTTCATTACAATGTATCTCAAAGGAGAAGTAGCAAATcttt                                                             >  3:171428/1‑137 (MQ=255)
                                                                     tCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCttttt                                                                                                   >  6:141862/1‑101 (MQ=255)
                                                                     tCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCttttt                                                                                                   <  5:141862/101‑1 (MQ=255)
                                                                     tCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCAGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAAAGTGTCTAAAAGGGGAATGAGCAAAGcttt                                                             >  3:6397/1‑137 (MQ=255)
                                                                     tCCTTTTTGATACATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGCGGAAGGAGCAAAGcttt                                                             >  5:138729/1‑137 (MQ=255)
                                                                      ccTTTTTGATTCATTTCGCAGAGCAAAAAATGGAGCCGCCGGCTTCCGATTTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGcttt                                                             <  5:56720/138‑3 (MQ=255)
                                                                       cTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCtttt                                                            <  5:138770/138‑1 (MQ=255)
                                                                                 cATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCCAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGc                                                 >  2:52067/1‑139 (MQ=255)
                                                                                         agagCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGtt                                         <  8:35356/139‑1 (MQ=255)
                                                                                            gCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTc                                      >  1:155150/1‑139 (MQ=255)
                                                                                             cacaAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATTCTGTGCAATTGGCTTGTGTGCCTCGCC‑TTTTCATTCCAATGTCTCAAAAAGGGGAAGGCGCAAAGCTTTTTAACATGATGGTTTTGGTTTtgtg                                    >  2:308161/1‑139 (MQ=255)
                                                                                             cacaAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTATATCAAAGGGGAATTACCAAAGCTTTTTAACATGATTCTTTTGTttttgt                                     >  6:168783/1‑137 (MQ=255)
                                                                                                   agggAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGcttct                               <  4:58851/137‑1 (MQ=255)
                                                                                                   aTGGAGCCGCCTGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTTACTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGcttct                               <  5:168783/139‑1 (MQ=255)
                                                                                                       aGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTAt                           >  7:190928/1‑139 (MQ=255)
                                                                                                         ccgccggcTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATTTATATCAAAGGGGAAGGAGCAAATCTTTTTACCATGATGCTTTTCTTTTTCGGCTGCTTTAttt                         >  6:37810/1‑139 (MQ=255)
                                                                                                          cgccggcTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTc                        <  5:248535/139‑1 (MQ=255)
                                                                                                          cgccggcTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTc                        <  7:175566/139‑1 (MQ=255)
                                                                                                            ccggcTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCg                      >  3:214496/4‑139 (MQ=255)
                                                                                                            ccggcTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTATTCATTCCAATGTCTCTCAAAGGGGAAGGAGATAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCGTTATTTACg                      >  3:90328/4‑139 (MQ=255)
                                                                                                               gcTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCt                   <  6:260158/139‑1 (MQ=255)
                                                                                                                     gAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAac             <  8:190928/139‑1 (MQ=255)
                                                                                                                          gTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAg         >  2:225082/1‑138 (MQ=255)
                                                                                                                           tttttCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGAGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCa       <  4:171428/139‑1 (MQ=255)
                                                                                                                                 agagGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCGTTGAACACAGCATTGcc  <  3:117379/138‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                    
TTGATCGGCGCCATTCTATTTGCCATTCTGATCAGCGCGACAGGGCTTTTTGAGGAGGCTTCTGTTCATTCCTTTTTGATTCATCTGGCAGAGCACAAAATGGAGCCGCCCGCTTCCGAACTGTTTTTCAGAGGGATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTTAAAGGGGAAGGGGCAAAGCTCTTTACCATGATGCTTTTCGTTTTCTGCTTCTTCATTTCCGGCTTCGAACACAGCATTGCC  >  CP006881/3723532‑3723798

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑