Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 856,396 C→T 7.8% T383I (ACA→ATA)  U712_04155 → PTS system maltose‑specific EIICB component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP006881856,3960CT 7.8% 156.7 / 3.1 51T383I (ACA→ATA) U712_04155PTS system maltose‑specific EIICB component
Reads supporting (aligned to +/- strand):  ref base C (26/21);  new base T (2/2);  total (28/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.08e-01

ATTTGCGGTTCACGCCGTGCTTGCCGCCACAATGTCGACAGTTATGTATATGGCCGGCGTCGTCGGAAATATGGGAGGCGGACTGATTGAGGCGGTAACCTTGAACTGGATTCCGCTCTTTGGCAGCCACGGTATGACATATGTGTATCAAATTTTGATCGGGCTCTCGTTTACAGCAATTTATTTTTTCGTCTTCAGATTTTTAATCCTCAAATTCAATATCGCTACACCAGGACGGGAAAAGGATGAACAGCAGGAAACAAAGCTATATTCGA  >  CP006881/856259‑856533
                                                                                                                                         |                                                                                                                                         
aTTTGCGGTTCACGCCGTGCTTGCCGCCACAATGGCGAAAGTTATGTATATGGCCGGCGTCGTCGGAAATATGGGAGGCGGACTGATTGAGGCGGTAACCTTGAACTGGATTCCGCTCTTTTGCAGCCACGGTATGAc                                                                                                                                           <  7:42422/138‑1 (MQ=255)
                  gCTTGCCGCCACAATGTCGACAGTTATGTATATGGCCGGCGTCGTCGGAAATATGGGAGGCGGACTGATTGAGGCGGTAACCTTGAACTGGATTCCGCTCTTTGGCAGCCACGGTATGACATATGTGTATCAAAtttt                                                                                                                         >  7:261262/1‑138 (MQ=255)
                   cTTGCCGCCACAATGTCGACAGTTATGTATATGGCCGGCGTCGTCTGAAATATGGGAGGCGGACTGATTGAGGCGGTAACCTTGAACTGGATTCCGCTCTTTGGCAGCCACGGTATGACATATGTGTATCAAATTTTGa                                                                                                                       <  5:37261/139‑1 (MQ=255)
                       ccgccACAATGTCGACAGTTATGTATATGGCCGGCGTCGTCGGAAATATGGGAGGCGGACTGATTGAGGCGGTAACCTTGAACTGGATTCCGCTCTTTGGCAGCCACGGTATGACATATGTGTATCAAATTTTGATCgg                                                                                                                   >  5:44039/1‑139 (MQ=255)
                          ccACAATGTCGACAGTTATGTATATGGCCGGCGTCGTCGGAAATATGGGAGGCGGACTGATTGAGGCGGTAACCTTGAACTGGATTCCGCTCTTTGGCAGCCACGGTATGACATATGTGTATCAAATTTTGATCGGGct                                                                                                                <  2:129580/139‑1 (MQ=255)
                                            tGTATATGGCCGGCGTCGTCGGAAATATGGGAGGCGGACTGATTGAGGCGGTAACCTTGAACTGGATTCCGCTCTTTGGCAGCCACGGTATGACATATGTGTATCAAATTTTGATc                                                                                                                     <  6:20866/116‑1 (MQ=255)
                                            tGTATATGGCCGGCGTCGTCGGAAATATGGGAGGCGGACTGATTGAGGCGGTAACCTTGAACTGGATTCCGCTCTTTGGCAGCCACGGTATGACATATGTGTATCAAATTTTGATc                                                                                                                     >  5:20866/1‑116 (MQ=255)
                                                 atGGCCGGCGTCGTCGGAAATATGGGAGGCGGACTGATTGAGGCGGTAACCTTGAACTGGATTCCGCTCTTTGGCAGCCACGGTATGACATATGTGTATCAAATTTTGATCGGGCTCTCGTTTACa                                                                                                      <  4:267609/126‑1 (MQ=255)
                                                 atGGCCGGCGTCGTCGGAAATATGGGAGGCGGACTGATTGAGGCGGTAACCTTGAACTGGATTCCGCTCTTTGGCAGCCACGGTATGACATATGTGTATCAAATTTTGATCGGGCTCTCGTTTACa                                                                                                      >  3:267609/1‑126 (MQ=255)
                                                 atGGCCGGCGTCGTCGGAAATATGGGAGGCGGACTGATTGAGGCGGTAACCTTGAACTGGATTCCGCTCTTTGGCAGCCACGGTATGACATATGTGTATCAAATTTTGATCGGGCTCTCGTTTACAGCAATTTAttttt                                                                                         >  2:125698/1‑139 (MQ=255)
                                                  tGGCCGGCGTCGTCGGAAATATGGGAGGCGGACTGATTGAGGCGGTAACCTTGAACTGGATTCCGCTCTTTGGCAGCCCCGGTATGACATATGTGTATCAAATTTTGATCGGGCTCTCGTTTACAGCAATTTAtttttt                                                                                        >  5:134318/1‑139 (MQ=255)
                                                   ggCCGGCGTCGTCGGAAATATGGGAGGCGGACTGATTGAGGCGGTAACCTTGAACTGGATTCCGCTCTTTGGCAGCCACGGTATGATATATGTGTATCAAATTTTGATCGGGctc                                                                                                               >  3:208394/1‑115 (MQ=255)
                                                   ggCCGGCGTCGTCGGAAATATGGGAGGCGGACTGATTGAGGCGGTAACCTTGAACTGGATTCCGCTCTTTGGCAGCCACGGTATGATATATGTGTATCAAATTTTGATCGGGctc                                                                                                               >  3:212629/1‑115 (MQ=255)
                                                   ggCCGGCGTCGTCGGAAATATGGGAGGCGGACTGATTGAGGCGGTAACCTTGAACTGGATTCCGCTCTTTGGCAGCCACGGTATGATATATGTGTATCAAATTTTGATCGGGctc                                                                                                               <  4:212629/115‑1 (MQ=255)
                                                   ggCCGGCGTCGTCGGAAATATGGGAGGCGGACTGATTGAGGCGGTAACCTTGAACTGGATTCCGCTCTTTGGCAGCCACGGTATGATATATGTGTATCAAATTTTGATCGGGctc                                                                                                               <  4:208394/115‑1 (MQ=255)
                                                   ggCCGGCGTCGTCGGAAATATGGGAGGCGGACTGATTGAGGCGGTAACCTTGAACTGGATTCCGCTCTTTGGCAGCCACGGTATGACATATGTGTATCAAATTTTGAt                                                                                                                      >  4:270860/1‑108 (MQ=255)
                                                   ggCCGGCGTCGTCGGAAATATGGGAGGCGGACTGATTGAGGCGGTAACCTTGAACTGGATTCCGCTCTTTGGCAGCCACGGTATGACATATGTGTATCAAATTTTGAt                                                                                                                      <  3:270860/108‑1 (MQ=255)
                                                   ggCCGGCGTCGTCGGAAATATGGGAGGCGGACTGATTGAGGCGGTAACCTTGAACTGGATTCCGCTCTTTGGCAGCCACGGTATGACATATGTGTATCAAATTTTGATCGGGCTCTCGTTTACAGCAATTTATTTTTTc                                                                                       >  5:1268/1‑139 (MQ=255)
                                                   ggCCGGCGTCGTCGGAAATATGGGAGGCGGACTGATTGAGGCGGTAACCTTGAACTGGATTCCGCTCTTTGGCAGCCACGGTATGACATATGTGTATCAAATTTTGATCGGGCTCTCGTTTACAGCAATTTATTTTTTc                                                                                       >  5:125203/1‑139 (MQ=255)
                                                   ggCCGGCGTCGTCGGAAATATGGGAGGCGGACTGATTGAGGCGGTAACCTTGAACTGGATTCCGCTCTTTGGCAGCCACGGTATGACATATGTGTATCAAATTTTGATCGGGCTCTCGTTTACAGCAATTTATTTTTTc                                                                                       >  4:113724/1‑139 (MQ=255)
                                                   ggCCGGCGTCGTCGGAAATATGGGAGGCGGACTGATTGAGGCGGTAACCTTGAACTGGATTCCGCTCTTTGGCAGCCACGGTATGACATATGTGTATCAAATTTTGATCGGGCTCTCGTTTACAGCAATTTATTTTTTc                                                                                       >  7:107372/1‑139 (MQ=255)
                                                   ggCCGGCGTCGTCGGAAATATGGGAGGCGGACTGATTGAGGCGGTAACCTTGAACTGGATTCCGCTCTTTGGCAGCCACGGTATGACATATGTGTATCAAATTTTGATCGGGCTCTCGTTTACAGCAATTTATTTTTTc                                                                                       >  7:166929/1‑139 (MQ=255)
                                                   ggCCGGCGTCGTCGGAAATATGGGAGGCGGACTGATTGAGGCGGTAACCTTGAACTGGATTCCGCTCTTTGGCAGCCACGGTATGACATATGTGTATCAAATTTTGATCGGGCTCTCGTTTACAGCAATTTATTTTTTc                                                                                       >  3:50877/1‑139 (MQ=255)
                                                   ggCCGGCGTCGTCGGAAATATGGGAGGCGGACTGATTGAGGCGGTAACCTTGAACTGGATTCCACTCTTTGGCAGCCACGGTATGACATATGTGTATCAAATTTTGATCGGGCTCTCGTTTACAGCAATTTATTTTTTc                                                                                       >  3:180243/1‑139 (MQ=255)
                                                        gCGTCGTCGGAAATATGGGAGGCGGCCTGATTGAGGCGGTAACCTTGCACTGGATTCCGCTCGTTGGCAGCCACGGGATGCCATATGTGTCTCAAAGTTTGATCGGGCTCGCGTGTACAGCAATTTATTTTTTCGTcgt                                                                                  >  2:74565/1‑137 (MQ=255)
                                                        gCGTCGTCGGAAATATGGGAGGCGGACTGATTGAGGCGGTAACCTTGAACTGGATTCCGCTCTTTGGCAGCCACGGTATGACATATGTGTATCAAATTTTGATCGGGCTCTCGTTTACAGCAATTTATTTTTTCGTCtt                                                                                  >  6:89611/1‑139 (MQ=255)
                                                        gCGTCGTCGGAAATATGGGAGGCGGACTGATTGAGGCGGTAACCTTGAACTGGATTCCGCTCTTTGGCAGCCACGGTATGACATATGTGTATCAAATTTTGATCGGGCTCTCGTTTACAGCAATTTATTTTTTCGTCtt                                                                                  >  8:284144/1‑139 (MQ=255)
                                                        gCGTCGTCGGAAATATGGGAGGCGGACTGATTGAGGCGGTAACCTTGAACTGGATTCCGCTCTTTGGCAGCCACGGTAAGACATATGTGTATCAAATTTTGATCGGGCTCTCGTGTACAGCAATTTATTTTTTCGTCt                                                                                   >  5:224659/1‑138 (MQ=255)
                                                                         ggAGGCGGACTGATTGAGGCGGTAACCTTGAACTGGATTCCGCTCTTTGGCAGCCACGGTATGACATATGTGTATCAAATTTTGATCGGGCTCTCGTTTACAGCAATTTATTTTTTCGTCTTCAGATTTTTAATCCTc                                                                  <  6:44039/138‑1 (MQ=255)
                                                                         ggAGGCGGACTGATTGAGGCGGTAACCTTGAACTGGATTCCGCTCTTTGGCAGCCACGGTATGACATATGTGTATCAAATTTTGATCGGGCTCTCGTTTACAGCAATTTATTTTTTCGTCTTCAGATTTTTAATCCTc                                                                  <  6:125203/138‑1 (MQ=255)
                                                                               ggACTGATTGAGGCGGTAACCTTGAACTGGATTCCGCTCTTTGGCAGCCACGGTATGACATATGTGTATCAAATTTTGATCGGGCTCTCGTTTACAGCAATTTATTTTTTCGTCTTCAGATTTTTAATCCTCAAATTc                                                            >  4:253657/1‑138 (MQ=255)
                                                                                gACTGATTGAGGCGGTAACCTTGAACTGGATTCCGCTCTTTGGCAGCCACGGTATGACATATGTGTATCAAATTTTGATCGGGCTCTCGTTTACAGCAATTTATTTTTTCGTCTTCAGATTTTTAATCCTCAAATTCaa                                                          <  8:166929/139‑1 (MQ=255)
                                                                                 aCTGATTGAGGCGGTAACCTTGAACTGGATTCCGCTCTTTGGCAGCCACGGTATGACATATGTGTATCAAATTTTGATCGGGCTCTCGTTTACAGCAATTTATTTTTTCGTCTTCAGATTTTTAATCCTCAAATTCAat                                                         <  3:113724/139‑1 (MQ=255)
                                                                                     aTTGAGGCGGTAACCTTGAACTGGATTCCGCTCTTTGGCAGCCACGGTATGACATATGTGTATCAAATTTTGATCGGGCTCTCGTTTACAGCAATTTATTTTTTCGTCTTCAGATTTTTAATCCTCAAATTCAATATCg                                                     <  8:261262/139‑1 (MQ=255)
                                                                                      ttGAGGCGGTAACCTTGAACTGGATTCCGCTCTTTGGCAGCCACGGTATGACATATGTGTATCAAATTTTGATCGGGCTCTCGTTTACAGCAATTTATTTTTTCGTCTTCAGATTTTTAATCCTCAAATTCAATATCGc                                                    <  3:253657/139‑1 (MQ=255)
                                                                                      ttGAGGCGGTAACCTTGAACTGGATTCCGCTCTTTGGCAGCCACGGTATGACATATGTGTATCAAATTTTGATCGGGCTCTCGTTTACAGCAATTTATTTTTTCGTCTTCAGATTTTTAATCCTCAAATTCAATATCGc                                                    <  1:204274/139‑1 (MQ=255)
                                                                                      ttGAGGCGGTAACCTTGAACTGGATTCCGCTCTTTGGCAGCCACGGTATGACATATGTGTATCAAATTTTGATCGGGCTCTCGTTTACAGCAATTTATTTTTTCGTCTTCAGATTTTTAATCCTCAAATTCAATATCGc                                                    <  5:89611/139‑1 (MQ=255)
                                                                                       tGAGGCGGTAACCTTGAACTGGATTCCGCTCTTTGGCAGCCACGGTATGACATATGTGTATCAAATTTTGATCGGGCTCTCGTTTACAGCAATTTATTTTTTCGTCTTCAGATTTTTAATCCTCAAATTCAATATCGc                                                    <  1:125698/138‑1 (MQ=255)
                                                                                           gCGGTAACCTTGAACTGGATTCCGCTCTTTGGCAGCCACGGTATGACATATGTGTATCAAATTTTGATCGGGCTCTCGTGTACAGCATTTTATTTTTTCTTCTTCTGAGTTTTAATCCTCAAATTCAATTTCGCTacac                                               >  1:139005/1‑139 (MQ=255)
                                                                                               tAACCTTGAACTGGATTCCGCTCTTTGGCAGCCACGGTATGACATATGTGTATCAAATTTTGATCGGGCTCTCGTTTACAGCAATTTATTTTTTCGTCTTCAGATTTTTAATCCTCAAATTCAATATCGCTACACCAgg                                           >  4:145857/1‑139 (MQ=255)
                                                                                               tAACCTTGAACTGGATTCCGCTCTTTGGCAGCCACGGTATGACATATGTGTATCAAATTTTGATCGGGCTCTCGTTTACAGCAATTTATTTTTTCGTCTTCAGATTTTTAATCCTCAAATTCAATATCGCTACACCAg                                            >  1:26028/1‑138 (MQ=255)
                                                                                                 aCCTTGAACTGGATTCCGCTCTTTGGCAGCCACGGTATGACATATGTGTATCAAATTTTGATCGGGCTCTCGTTTACAGCAATTTATTTTTTCGTCTTCAGATTTTTAATCCTCAAATTCAATATCGCTACACCAGGa                                          <  6:298506/138‑1 (MQ=255)
                                                                                                      gAACTGGATTCCGCTCTTTGGCAGCCACGGTATGACATATGTGTATCAAATTTTGATCGGGCTCTCGTTTACAGCAATTTATTTTTTCGTCTTCAGATTTTTAATCCTCAAATTCAATATCGCTACACCAGGACGGGa                                     <  2:19321/138‑1 (MQ=255)
                                                                                                            gATTCCGCTCTTTGGCAGCCACGGTATGACATATGTGTATCAAATTTTGATCGGGCTCTCGTTTACAGCAATTTATTTTTTCGTCTTCAGATTTTTAATCCTCAAATTCAATATCGCTACACCAGGACGGGAAAAGGAt                              >  3:246343/1‑139 (MQ=255)
                                                                                                             aTTCCGCTCTTTGGCAGCCACGGTATGACATATGTGTATCAAATTTTGATCGGGCTCTCGTTTACAGCAATTTATTTTTTCGTCTTCAGATTTTTAATCCTCAAATTCAATATCGCTACACCAGGACGGGAAAAGGATg                             <  1:57007/139‑1 (MQ=255)
                                                                                                                ccGCTCGTTGGCAGCCACGGTATGACATATGTGTATCAAATTTTGCTCGGGCTCTCGTTTACAGCAATTAATATTTTCGTCTTCATATTTAAAATCCTCAAATTCAATATCGCTACACCAGGACGGGAAAAGGATGaa                           <  4:248968/138‑1 (MQ=255)
                                                                                                                      tttGGCAGCCACGGTATGACATATGTGTATCAAATTTTGATCGGGCTCTCGTTTACAGCAATTTATTTTTTCGTCTTCAGATTTTTAATCCTCAAATTCAATATCGCTACACCAGGACGGGAAAAGGATGAAcagca                      <  7:284144/137‑1 (MQ=255)
                                                                                                                                aCGGTATGACATATGTGTATCAAATTTTGATCGGGCTCTCGTTTACAGCAATTTATTTTTTCGTCTTCAGATTTTTAATCCTCAAATTCAATATCGCTACACCAGGACGGGAAAAGGATGAACAGCAGGAAACAAAGc           >  7:122637/1‑138 (MQ=255)
                                                                                                                                    tATGACATATGTGTATCAAATTTTGATCGGGCTCTCGTTTACAGCAATTTATTTTTTCGTCTTCAGATTTTTAATCCTCAAATTCAATATCGCTACACCAGGACGTGAAAAGGATGAACAGCAGGAAACACAGCTatat      >  8:305223/1‑139 (MQ=255)
                                                                                                                                      tGACATATGTGTATCAAATTTTGATCGGGCTCTCGTTTACAGCAATTTATTTTTTCGTCTTCAGATTTTTAATCCTCAAATTCAATATCGCTACACCAGGACGGGAAAAgg                                <  4:139309/111‑1 (MQ=255)
                                                                                                                                      tGACATATGTGTATCAAATTTTGATCGGGCTCTCGTTTACAGCAATTTATTTTTTCGTCTTCAGATTTTTAATCCTCAAATTCAATATCGCTACACCAGGACGGGAAAAgg                                >  3:139309/1‑111 (MQ=255)
                                                                                                                                        aCATATGTGTATCAAATTTTGCTCGGGCTCTCGTTTACAGCAATTTATTTTTTCGTCTTCAGAATTTTAATCCTCAAATTCAAAATCGCTACACCACGCCGGGAAAAGGATGAACAGCAGGAAACAAAGCTATATTCGa  <  4:180243/139‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                         
ATTTGCGGTTCACGCCGTGCTTGCCGCCACAATGTCGACAGTTATGTATATGGCCGGCGTCGTCGGAAATATGGGAGGCGGACTGATTGAGGCGGTAACCTTGAACTGGATTCCGCTCTTTGGCAGCCACGGTATGACATATGTGTATCAAATTTTGATCGGGCTCTCGTTTACAGCAATTTATTTTTTCGTCTTCAGATTTTTAATCCTCAAATTCAATATCGCTACACCAGGACGGGAAAAGGATGAACAGCAGGAAACAAAGCTATATTCGA  >  CP006881/856259‑856533

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑