Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP006881	3,723,853	G→C	100%	V223M (GTA→CTG)	U712_19195 →	putative transporter ywcJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	3,723,853	0	G	C	100.0%	249.5 / NA	66	V223M (GTA→CTG)	U712_19195	putative transporter ywcJ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (35/31);  total (35/31)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GAAGGGGCAAAGCTCTTTACCATGATGCTTTTCGTTTTCTGCTTCTTCATTTCCGGCTTCGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCACTTTTGATCGAGCACCCTGATACAGTGACAGTAATGGGAGCAGTCAGAAACTTAATTCCCGTTACACTCGGCAACCTGACCGCGGGAATGGTCATGATGGGCTGGATGTATTACACGCTCAATCCCGATAAATAAAAAAACTTCCAGAGATCACTCCCTGCAAG  >  CP006881/3723724‑3723986                                                                                                                                  |                                                                                                                                      gaaggagCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAg                                                                                                                              >  8:198832/1‑139 (MQ=255) gaaggagCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAg                                                                                                                              <  2:168058/139‑1 (MQ=255)  aaggagCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAg                                                                                                                              >  3:248819/1‑138 (MQ=255)                tttACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGa                                                                                                                      <  6:39399/132‑1 (MQ=255)                tttACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGa                                                                                                                      >  5:39399/1‑132 (MQ=255)                      atgatgCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCg                                                                                                         <  3:198099/139‑1 (MQ=255)                       tgatgCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAAt                                                                                                              <  2:234836/133‑1 (MQ=255)                       tgatgCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAAt                                                                                                              >  1:234836/1‑133 (MQ=255)                       tgatgCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGt                                                                                                        >  1:175130/1‑139 (MQ=255)                       tgatgCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGt                                                                                                        >  8:148140/1‑139 (MQ=255)                       tgatgCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGt                                                                                                        >  5:280897/1‑139 (MQ=255)                               ttCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCg                                                                                                >  5:106125/1‑139 (MQ=255)                                          cttcttTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGAc                                                                                      <  1:125899/138‑1 (MQ=255)                                                  tttCCGGCGTTGCACAAAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGCTACAGTGACACTGATGGGAGCAGGCAGAAACTTAATCCCCGTTACGCGCGGCAATCTGACCGCGGGaa                                                                              <  2:302209/138‑1 (MQ=255)                                                    tCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAAt                                                                                                              <  7:48789/104‑1 (MQ=255)                                                    tCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAAt                                                                                                              >  8:48789/1‑104 (MQ=255)                                                      cGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGtt                                                                         <  6:45140/139‑2 (MQ=255)                                                          cttgAACACAGCATTGCCAATATGTGCACATTCGCCATCTCTCTTTTGATCGAGCACACTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTatga                                                                     <  4:90328/136‑1 (MQ=255)                                                            tgAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTatga                                                                     <  1:248666/136‑1 (MQ=255)                                                                  caGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTgg                                                             <  6:106125/139‑1 (MQ=255)                                                                  caGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTgg                                                             <  4:248819/139‑1 (MQ=255)                                                                  caGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTgg                                                             <  2:175130/139‑1 (MQ=255)                                                                    gCATTGCCAATATGTGCACATTCGCCATCTCTCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGAt                                                           <  7:198832/139‑1 (MQ=255)                                                                    gCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGAt                                                           <  6:280897/139‑1 (MQ=255)                                                                    gCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGAt                                                           <  7:103025/139‑1 (MQ=255)                                                                    gCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGAt                                                           <  7:98520/139‑1 (MQ=255)                                                                        tGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGa                                                                                                                      <  4:208015/76‑1 (MQ=255)                                                                        tGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGa                                                                                                                      >  3:208015/1‑76 (MQ=255)                                                                          ccAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTa                                                     <  3:66762/139‑1 (MQ=255)                                                                           cAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGtactac                                                    <  1:123932/139‑1 (MQ=255)                                                                             atatGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAAt                                                                                                              <  4:41703/79‑1 (MQ=255)                                                                             atatGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAAt                                                                                                              >  3:41703/1‑79 (MQ=255)                                                                             atatGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGaa                                                                              >  2:189800/1‑111 (MQ=255)                                                                             atatGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGaa                                                                              <  1:189800/111‑1 (MQ=255)                                                                               atGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAAt                                                                                                              <  1:98947/77‑1 (MQ=255)                                                                               atGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAAt                                                                                                              >  2:98947/1‑77 (MQ=255)                                                                                    cacaTTCGCCATCTCGCTTTTGATCGAGCCCCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGaa                                                                              >  7:220895/1‑104 (MQ=255)                                                                                    cacaTTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCa                                                                                                                        >  3:140514/1‑62 (MQ=255)                                                                                    cacaTTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCa                                                                                                                        <  4:140514/62‑1 (MQ=255)                                                                                    cacaTTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAAt                                           >  3:221017/1‑139 (MQ=255)                                                                                    cacaTTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAAt                                           >  7:293725/1‑139 (MQ=255)                                                                                         tCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACCCACTGAATCCTGa                                      >  2:206426/1‑139 (MQ=255)                                                                                         tCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGACCAGTCAGAAACTTAATCCCCGATACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGa                                      >  5:298320/1‑139 (MQ=255)                                                                                         tCGCCAGCTCGCTTTTGATCGAGCACCCTGATACAGTGACACGGTTGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATTGTTATGATGGGCTGGATGTACTACACACTGAATCCTGa                                      >  3:72741/1‑139 (MQ=255)                                                                                         tCGCCAACTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACcgc                                                                                   <  8:166574/94‑1 (MQ=255)                                                                                         tCGCCAACTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACcgc                                                                                   >  7:166574/1‑94 (MQ=255)                                                                                                         gATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTgg                                                             <  7:294052/100‑1 (MQ=255)                                                                                                         gATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTgg                                                             >  8:294052/1‑100 (MQ=255)                                                                                                             gAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCa                    >  3:227872/1‑137 (MQ=255)                                                                                                                       aTACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGaa                                                                              <  5:20192/69‑1 (MQ=255)                                                                                                                       aTACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGaa                                                                              >  6:20192/1‑69 (MQ=255)                                                                                                                             tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCATTCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCa                    <  1:257157/121‑1 (MQ=255)                                                                                                                             tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCATTCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCa                    >  2:257157/1‑121 (MQ=255)                                                                                                                             tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGaa                                                                              >  7:151459/1‑63 (MQ=255)                                                                                                                             tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGaa                                                                              <  8:151459/63‑1 (MQ=255)                                                                                                                             tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTa                                                     <  8:55297/88‑1 (MQ=255)                                                                                                                             tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTa                                                     >  7:55297/1‑88 (MQ=255)                                                                                                                             tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACt                                               >  4:301821/1‑94 (MQ=255)                                                                                                                             tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACt                                               <  3:301821/94‑1 (MQ=255)                                                                                                                             tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAg  >  7:202449/1‑139 (MQ=255)                                                                                                                             tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAg  >  6:84109/1‑139 (MQ=255)                                                                                                                             tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAg  >  6:271419/1‑139 (MQ=255)                                                                                                                             tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAg  >  4:117913/1‑139 (MQ=255)                                                                                                                             tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAg  >  1:136214/1‑139 (MQ=255)                                                                                                                             tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAg  >  1:106656/1‑139 (MQ=255)                                                                                                                             tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAg  >  8:99200/1‑139 (MQ=255)                                                                                                                                  |                                                                                                                                      GAAGGGGCAAAGCTCTTTACCATGATGCTTTTCGTTTTCTGCTTCTTCATTTCCGGCTTCGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCACTTTTGATCGAGCACCCTGATACAGTGACAGTAATGGGAGCAGTCAGAAACTTAATTCCCGTTACACTCGGCAACCTGACCGCGGGAATGGTCATGATGGGCTGGATGTATTACACGCTCAATCCCGATAAATAAAAAAACTTCCAGAGATCACTCCCTGCAAG  >  CP006881/3723724‑3723986

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑