Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP006881	3,724,623	A→T	100%	I68I (ATT→ATA)	U712_19200 ←	SacPA operon antiterminator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	3,724,623	0	A	T	100.0%	160.8 / NA	48	I68I (ATT→ATA)	U712_19200	SacPA operon antiterminator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (22/26);  total (22/26)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCAGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCCCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCAATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTAAACTTTTCATTCTCGTCGTGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGGTCATTTTTCTTTTTTTGAAAAGCGATTCCTGGTCCCAT  >  CP006881/3724489‑3724757                                                                                                                                       |                                                                                                                                       ttCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGGCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTatat                                                                                                                                    <  7:82770/139‑1 (MQ=255)      cacaAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGctc                                                                                                                                <  3:127815/138‑1 (MQ=255)       acaAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGCGCTCTCCTTCCGCATAGGTGATGATTTCCTCATCTATTTCTATATGctcct                                                                                                                              >  5:190974/1‑139 (MQ=255)           gcagcCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCgg                                                                                                                          >  8:290668/1‑139 (MQ=255)            cagcCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGc                                                                                                                         <  3:54807/139‑1 (MQ=255)              gcCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCCGCGGCGCCGCGCGGTCTCCTTCCGCATAGCTGATGATATCCTCAGCTAGTTCTATATGCTCCTCCGGCAg                                                                                                                       >  3:90042/1‑139 (MQ=255)                     ttGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTg                                                                                                                 <  7:290668/138‑1 (MQ=255)                         aTCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGa                                                                                                            >  4:39706/1‑139 (MQ=255)                         aTCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGa                                                                                                            >  3:225906/1‑139 (MQ=255)                         aTCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGAGCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGa                                                                                                            <  3:71225/139‑1 (MQ=255)                                  tgcGCAAAGGTGAAATGGTCGGAAAGCGCGATATGAATGTGTTCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGtttga                                                                                                   <  6:179737/137‑3 (MQ=255)                                  aTCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGtttga                                                                                                   <  6:261075/139‑3 (MQ=255)                                                   gTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGc                                                                                                                                  >  6:179441/1‑91 (MQ=255)                                                   gTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGc                                                                                                                                  <  5:179441/91‑1 (MQ=255)                                                     cggAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCtcgtcgcg                                                                                <  1:258783/137‑3 (MQ=255)                                                      ggAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCtcgtcgcg                                                                                >  1:104214/2‑136 (MQ=255)                                                       gAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCa                                                                              <  3:39706/139‑1 (MQ=255)                                                       gAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCa                                                                              <  3:221099/139‑1 (MQ=255)                                                       gAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCa                                                                              <  7:125128/139‑1 (MQ=255)                                                        aaaGCGCGATATGAATGTGATCGCGCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCAc                                                                             <  5:188906/139‑1 (MQ=255)                                                        aaaGCGCGATATGAATGCGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCAc                                                                             <  5:184905/139‑1 (MQ=255)                                                               gagatGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATCTGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAg                                                                      <  8:82457/136‑1 (MQ=255)                                                                         tgATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTcc                                                             <  1:140697/138‑1 (MQ=255)                                                                            tCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACt                                                          <  4:35446/138‑1 (MQ=255)                                                                             cGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACtt                                                         <  6:190974/138‑1 (MQ=255)                                                                               cTCAGCGGCGCCGCGCGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGGAGTGTTTGGAGGGTTTGTTTGTACTTTTCATTATCGTCGCGCACGACAAAGATGGTTTGCACttttt                                                      >  1:153007/1‑137 (MQ=255)                                                                               cTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTAt                                                      <  7:43069/139‑1 (MQ=255)                                                                               cTCAGCGGCGCCGCGAGCTCGCCTTCCGCATAGCGGCTGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTGGTTTGTAGTTTTGATTCTCGTCGCGCCCCACCAAGATCTTTTCCACgtttt                                                      >  4:178105/1‑137 (MQ=255)                                                                                   gcggcgCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTTTATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGATCTTTTCATTCTCGTCTCGCACGACAAAGATCTTTACCACTTTATTCAt                                                  <  1:111819/139‑1 (MQ=255)                                                                                         ccgcGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGAt                                            >  2:65234/1‑139 (MQ=255)                                                                                                           cATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACa                                                                         >  8:37597/1‑92 (MQ=255)                                                                                                           cATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACa                                                                         <  7:37597/92‑1 (MQ=255)                                                                                                           cATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCttttt                          >  4:32604/1‑139 (MQ=255)                                                                                                           cATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCttttt                          >  3:181170/1‑139 (MQ=255)                                                                                                           cATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACATTATTAATAGGGATGAGATCATTTTTCttttt                          >  1:251001/1‑139 (MQ=255)                                                                                                             tAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCttttttt                        >  6:174838/1‑139 (MQ=255)                                                                                                                cTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTGTGAACTTTTCATTCTCGTCGCGCACGCCAAAGATCTTTTCCACTTTATTCATTGGGATGAGATCATTTTTCTTTTTTTGa                      >  8:89578/1‑138 (MQ=255)                                                                                                                   atgatATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCtttt                                                               >  5:72411/1‑94 (MQ=255)                                                                                                                   atgatATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCtttt                                                               <  6:72411/94‑1 (MQ=255)                                                                                                                   atgatATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACt                                                          >  8:95281/1‑99 (MQ=255)                                                                                                                   atgatATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACt                                                          <  7:95281/99‑1 (MQ=255)                                                                                                                          ccTCAGCTATTTCTATATGCTCCTCCGGCAGTGGTGGCAGGATTTGTTCGAACTTTTCATTCTCGGCGCCCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTcc           <  6:293725/139‑1 (MQ=255)                                                                                                                              aGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGt       >  1:38384/1‑139 (MQ=255)                                                                                                                              aGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGt       >  1:33674/1‑139 (MQ=255)                                                                                                                              aGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGt       <  2:33674/139‑1 (MQ=255)                                                                                                                              aGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGt       <  2:38384/139‑1 (MQ=255)                                                                                                                                cTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTcc     >  5:253093/1‑139 (MQ=255)                                                                                                                                   tttCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCAt  >  1:96944/1‑139 (MQ=255)                                                                                                                                       |                                                                                                                                       TTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCAGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCCCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCAATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTAAACTTTTCATTCTCGTCGTGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGGTCATTTTTCTTTTTTTGAAAAGCGATTCCTGGTCCCAT  >  CP006881/3724489‑3724757

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑