| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP006881 | 3,725,237 | A→G | 100% | intergenic (‑69/‑460) | U712_19205 ← / → U712_19210 | Uncharacterized protein ywcI/Minor extracellular protease vpr |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 3,725,237 | 0 | A | G | 91.3% | 67.9 / ‑4.8 | 28 | intergenic (‑69/‑460) | U712_19205/U712_19210 | Uncharacterized protein ywcI/Minor extracellular protease vpr |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (20/5); minor base C (0/3); total (20/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.71e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑C‑TTTT‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCACGGGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAACCAACTAGTTTTTAG > CP006881/3725110‑3725363 | gTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTTTTT‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGa < 7:178287/139‑1 (MQ=255) ttCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCGGCTTTTTGGCAGGCAGC‑CTTTTT‑A‑GCAGCCCGGGTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGAtg > 3:289871/1‑138 (MQ=255) aGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCCGGCAGC‑CTTTTT‑A‑GCCGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGAGTTCTTTTCTCg > 7:201611/1‑139 (MQ=255) aGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTTTTT‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGCCGGCCGATGTCTTTTCTCg > 8:141885/1‑139 (MQ=255) tAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTTTTT‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGCCGGCCGATTTCTTTTATCGCAAACTAGCGGGTCTa > 4:65951/1‑139 (MQ=255) tAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTTTTT‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGAGTTCTTTTCTCGCAAACTAGCGGctc > 1:165876/1‑137 (MQ=255) aGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGAGTCTTTTTGGCTGGCAGC‑CTTTTT‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGAAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCa < 8:67399/139‑1 (MQ=255) aCAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCCGC‑CTTTTT‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGCGTTGATTTGTCGGCAACTAGCCGGGGTAGCaaaa > 1:132172/1‑138 (MQ=255) aCAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCCGC‑CGTTTT‑T‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCCAGAAAAGACGGCCGATTTCGTTTGTCGCAAACTTGCGGCTCTTGCaaaa > 3:18788/1‑138 (MQ=255) aCAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTTTTT‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTACCGGCTCGAGCAAAAc > 8:83991/1‑139 (MQ=255) aCAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTTTTT‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTAGCAAACTAGCGGCTCTAGCaaaa > 8:242659/1‑138 (MQ=255) aaGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTTTTT‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGACAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCGCTAGCAAAACt > 7:134659/1‑138 (MQ=255) aaGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTTTTT‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACt > 1:76065/1‑138 (MQ=255) aaGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTTTTT‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTATCGCAAACTAGCGGCTCTAGCAAAACt > 7:173887/1‑138 (MQ=255) aGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTTGCAGGCAGC‑CTTTTT‑A‑GCAGCCCGTTTTCTCAGGCGCACCCCGCAAGAAAAGACGGCCGATTTATTTTGTAGCAACCAAGCGGCTGGAACAAAAcaaa > 6:3270/1‑136 (MQ=255) aGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTTTTT‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGCCGGCCGCTTTGTTTTCTCGCAAACGATCGGAGCTAGCaaaagtga > 6:103842/1‑135 (MQ=255) ccAAAGGTTTCATACAAAAACACCCCACCGCTTCTTTTTGCCCGCCCCC‑T‑TTTT‑ACCCCGCCCGTTTACTCCGCCCCCCCCCCCAATAAACCACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCAt < 3:230315/138‑1 (MQ=255) caaaccGTTCCATACAAAAACAGCCCACCGCTTCTTTTGGGCAGCCCCCTT‑TTTT‑A‑CCCCCCGGTTTCCTCCCCCGCACCCCCAAACAAACCACGGCCGATTTCTTTTCTCGAAAACTAGCGGCTCTAGCAAAACTCAt < 6:262056/134‑1 (MQ=255) aaaCTTTTAATATAAAAAAATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTTTTT‑A‑GCCGCCCTTTTTCTCAGCCGCAGCCCGAAATAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGa < 7:246969/139‑1 (MQ=255) aaaCGTTTCATAAAAAAACATCCCTCCGCTTTTTTTTGGCTGGCAGC‑CTTTTT‑A‑GCCGCCGTTTTTGTCCGCCGCAGCCCGCAATAAACGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGa < 3:145451/139‑1 (MQ=255) ccGTTACAAACAAAAACACCCCTCCGCTTCTTTTTGCCCGCCCCC‑C‑TTTTAA‑CCCGCCCGTTTTCCCCCCCCCCCCCCCAAACAAACCACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGAct < 2:10077/138‑1 (MQ=255) acttttAATACAAAAACATCCCACCGCTTCTTTTTGGCTCGCAGCGT‑TTTT‑A‑ACAGCCGGTTTTCTCAGCCCCCGCCCGCAAGAAACGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGAct < 7:192021/136‑1 (MQ=255) aCGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTTTTT‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATGTCTTTTCTCGCAAACGTCCGGCTCAAGCAAAACTCCTGAct > 3:56788/1‑139 (MQ=255) aCGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CATTTT‑A‑GCAGCCCGTTTTCTAAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTATTCTCGCAAACTAGCGGCTAAAGAAAAACACATGCct > 4:253740/1‑139 (MQ=255) ttCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTTTTT‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTATATTCTCGCAAACTAGCGGCTCTAGCAAAACTCAAGACTCAAt > 7:200463/1‑139 (MQ=255) cccTCCGCTTCTTTTTGGCAGGCAGC‑CTTTTT‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCATTTATCGCAAACAAGCCGCTCGAGCAAAACTCATTCATCTATTATAGACCGTggga > 5:189382/1‑136 (MQ=255) cccGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGAGTTCTTTTCTCGCAAACTAGCGGCGCGTGCAAAACGCATGACTCTATGCTAGTCCGTGGAAAGAGAATTCTTTTTCACAATATGAGTGAAAAAACCAAc > 5:97465/1‑139 (MQ=255) cAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAACCAAC‑AGTTTTTAg > 7:288240/1‑138 (MQ=255) | GTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑C‑TTTT‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCACGGGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAACCAACTAGTTTTTAG > CP006881/3725110‑3725363 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |