BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	pSIJ663_pHT315_araE_xy	111	T→A	5.0%	intergenic (–/‑2867)	– / → eryR	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	pSIJ663_pHT315_araE_xy	111	0	T	A	5.0%	9564.2 / 67.2	3400	intergenic (–/‑2867)	–/eryR	–/–
Reads supporting (aligned to +/- strand): ref base T (1362/1813); new base A (7/214); total (1370/2030)
Fisher's exact test for biased strand distribution p-value = 1.71e-40
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 3424 total aligned reads displayed.

‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCC  >  pSIJ663_pHT315_araE_xy/1‑249

ccctgataaatgcttcaataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTaa                                                                                                                                     >  6:14386‑M1/22‑139 (MQ=255)

     ataaatgcttcaataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGATCCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGaa                                                                                                                                <  1:166790‑M1/123‑1 (MQ=255)

     ataaatgcttcaataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGTTAAAATTAGCAGTAACTTaa                                                                                                                                     <  4:134373‑M1/118‑1 (MQ=255)

     ataaatgcttcaataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGaa                                                                                                                                <  7:159863‑M1/123‑1 (MQ=255)

     ataaatgcttcaataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGaa                                                                                                                                <  8:294757‑M1/123‑1 (MQ=255)

     ataaatgcttcaataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGaa                                                                                                                                <  6:311849‑M1/123‑1 (MQ=255)

     ataaatgcttcaataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGaa                                                                                                                                <  1:49393‑M1/123‑1 (MQ=255)

      taaatgcttcaataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAATTTAGCAGTAACTTAATAGaa                                                                                                                                >  2:271645‑M1/16‑138 (MQ=255)

          tgcttcaataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGa                                                                                                                            <  7:127874‑M1/127‑1 (MQ=255)

          tgcttcaataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGa                                                                                                                            <  3:272643‑M1/127‑1 (MQ=255)

          tgcttcaataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGa                                                                                                                            <  2:296445‑M1/127‑1 (MQ=255)

           gcttcaataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGaaa                                                                                                                          <  2:992‑M1/129‑1 (MQ=255)

            cttcaataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGaaa                                                                                                                          >  7:19156‑M1/10‑138 (MQ=255)

               caataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGa                                                                                                                       <  4:186622‑M1/132‑1 (MQ=255)

               caataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGa                                                                                                                       <  3:40935‑M1/132‑1 (MQ=255)

               caataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGa                                                                                                                       <  7:246847‑M1/132‑1 (MQ=255)

                       cATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCTCAATTATTAATGTTTATGGTGAACCTTATCAAATTTTCTTTGATTTAATAGAAACATAGCGGTAAAATTAGCATTAACTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                              <  6:128524/139‑1 (MQ=255)

                       cATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATTTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATACAACGCAAATGAAAAAAGCCa                                                                                                              <  5:307736/139‑1 (MQ=255)

                       cATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                              <  5:86355/139‑1 (MQ=255)

                       cATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAAATTAATAGAACGGAAATGAAAAAAGCCa                                                                                                              <  1:214111/139‑1 (MQ=255)

                       cATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATAAGCAGTAACTAAATAGAACGTAAATGAAAAAAGCCa                                                                                                              <  2:73160/139‑1 (MQ=255)

                       aaTCCTCCAAAGTTGGAGAGTTAGTTTTAGTTCGAAAATATTATTGTTTCTGGTGAAACTTATAAAATTTTCGTTGTTTTATTATAACAATTGCGTTAAATTTAGCAGAAACTTAATACAACGGAAATTAAAAAAGCCa                                                                                                              <  2:225991/138‑1 (MQ=255)

                       aaTCCTCCAAAGTTGGACAGTGAGTTTTATTGCGCAAATATTAATGTTTCTGGGGAACCTTATCAAATTTTCGTTCATTAAATACAACCATCGCGTTAAAATTACCAGAAACTTAATACAACGCAAATCAAAAAAGCCa                                                                                                              <  6:273853/138‑1 (MQ=255)

                         tcctccAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                              <  3:301033/137‑1 (MQ=255)

                         tcctcaAACGTTGGAGAGTGATTTTTATGTCGCAAAAATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTTATTAAATAGAAACATAGCGTTAAAATTAGCAGTAAATTAAAACAACGTAAATTAAAAAAGCCAct                                                                                                            <  2:48876/139‑1 (MQ=255)

                          cctccAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCActc                                                                                                           >  7:35867/1‑139 (MQ=255)

                              cAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCat                                                                                                       <  3:99762/139‑1 (MQ=255)

                               aaaGGTGGAGAGTGAGTTTTATTCCGCAAATATTAATGTTTCTTGTTAACCTTATCAAATTTTCGTTGATTTAATAGAAAAATAGCGGAAAAATTCGCAGAAACTTAATAGACCGTAAATGAAAAAAGCCACTCTCata                                                                                                      <  6:290735/139‑1 (MQ=255)

                                aaGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCatat                                                                                                     >  5:287196/1‑139 (MQ=255)

                                   ttGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCAct                                                                                                            <  1:191427/129‑1 (MQ=255)

                                     ggAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTAt                                                                                                >  7:9152/1‑139 (MQ=255)

                                      gagaGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCAct                                                                                                            <  1:236510/126‑1 (MQ=255)

                                      gagaGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGcctgt                                                                                               >  3:249689/1‑135 (MQ=255)

                                      gagaGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTAtt                                                                                               >  7:102517/1‑139 (MQ=255)

                                      gagaGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTAtt                                                                                               >  1:71618/1‑139 (MQ=255)

                                      gagaGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTAtt                                                                                               >  1:259623/1‑139 (MQ=255)

                                      gagaGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTAtt                                                                                               >  6:800/1‑139 (MQ=255)

                                           tgagtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGATACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAACGCCACTCTCATATGCTATTGGCtt                                                                                          >  7:203349/1‑138 (MQ=255)

                                           tgagtAATATGTCGCAAATATTAAAGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGAAACTTAATAGAACGGAAATGAAAAACGCCACTCTCATAACCTATTGGATa                                                                                          >  8:3920/1‑139 (MQ=255)

                                             agtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTTATTAAAAAGAAACATAGCGGAAAAATTAGAAGTAACTTAATAGAACGCAAATGAAAAAAGCCACTCTCATATGCTATTGGCTAcc                                                                                        <  1:100421/139‑1 (MQ=255)

                                             agtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTgctgtct                                                                                        >  3:150900/1‑133 (MQ=255)

                                             agtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTAc                                                                                         >  2:171938/1‑138 (MQ=255)

                                             agtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTAc                                                                                         >  6:222640/1‑138 (MQ=255)

                                             agtTTTATGTCCCAAAAATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATCGAAACATCGCGGAAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTAcc                                                                                        <  5:241751/139‑1 (MQ=255)

                                              gtTTTATGTCGAAAATATTAATGTTTCTGGTTAACCTTATAAAATTTTCGTTTATTTAATAGAAACATAGCGGTAAAATTCGCAGTAAATTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTAcc                                                                                        <  3:116448/138‑1 (MQ=255)

                                                   aTGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCtt                                                                                  >  4:301329/1‑139 (MQ=255)

                                                     gTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTGGATTTAATAGAATCTTAGCGGTAAAATTAGCAGTAACTTAATATAACGGATATGATAAAAGACAGTCTCATATGATATTTGATAGCAAGCta                                                                                  >  8:81770/1‑136 (MQ=255)

                                                       cGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGATAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGc                                                                              >  1:145892/1‑139 (MQ=255)

                                                        gCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGc                                                                              <  2:204340/138‑1 (MQ=255)

                                                         cAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCAct                                                                                                            >  4:70637/1‑107 (MQ=255)

                                                         cAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGc                                                                              >  8:62882/1‑137 (MQ=255)

                                                         cAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGc                                                                              >  5:197767/1‑137 (MQ=255)

                                                         cAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGc                                                                              >  7:51380/1‑137 (MQ=255)

                                                            tttttAATTTTTCTGTTTATCATTATATAATTTTCGTTGTATAAAAATAACAATTGCGTTAAAATTTGCTTTAAATATTTAGTACGCATTTTAAATAAGCCACTCTCATATGCTATTGGCTACGAACCTTTAGCGAGaa                                                                         <  8:217533/136‑1 (MQ=255)

                                                            atatTAATTTTTGTGGTTAAAATTATAAATTTTTATTTGTTTTAATTCAAAAATCTCTTTAAAATTACCAGAATCTAAATAGAACGAAAATAAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCgagat                                                                         <  8:195757/139‑2 (MQ=255)

                                                            atatTAATGTTTTTGTTTAACCTTATAAAATTTTCGTTGATTTAATATAAACATAGCGGTAAAATTAGCAGAAAATTAATAGACCGAAAATCAAAAACGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                         <  5:175382/139‑1 (MQ=255)

                                                            atatTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCATTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                         <  3:168373/139‑1 (MQ=255)

                                                            atatTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                         <  1:30481/139‑1 (MQ=255)

                                                            atatTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                         <  7:222878/139‑1 (MQ=255)

                                                                  ttttttCTGGTTAACCTTATAAAATTTTCGTTTATTTAATAGAAACATAGCGGTAAAATAAGCATTAACTTAATAGAACGCAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACt                                                                    <  6:244523/135‑1 (MQ=255)

                                                                     tttCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTaa                                                                 <  2:25049/138‑1 (MQ=255)

                                                                     tttCTGGTGAAACTTATAAAATTTTCGTTTTTTTAATATAAACAAAGCTGTAAAATTAGCAGTAACTAAATATAACGGAAATTAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTaa                                                                 <  4:244109/138‑1 (MQ=255)

                                                                     tctgtGGTGAACCTTATTAATTTTTGGTTTATTAAAAACAAACATAGCGTTAAAATTATCTTTAAGTAAATCTAACGTAATTCAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTaa                                                                 <  2:120844/134‑1 (MQ=255)

                                                                      ttCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTCCCAACCTTTAGCGAGAATGACTTAATc                                                               >  5:46393/1‑139 (MQ=255)

                                                                       tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATcc                                                              >  6:16474/1‑139 (MQ=255)

                                                                       tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATcc                                                              >  1:94277/1‑139 (MQ=255)

                                                                       tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATcc                                                              >  5:103839/1‑139 (MQ=255)

                                                                       tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATcc                                                              >  4:45814/1‑139 (MQ=255)

                                                                       tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATcc                                                              >  7:271528/1‑139 (MQ=255)

                                                                       tCTGGTGAACCTTATCAAATTTTCGTTGAGTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCAACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATcc                                                              >  3:198261/1‑139 (MQ=255)

                                                                       tCTGGTGAACCTAATAAAATTTTCGTTGATTTAATAGAAACATATCTGTAAAATTAGAAGTAACTTAATAGAACGCAAATCAAAAAAGCCACTCACATATGCTATTGGCTACCAACCTTTAGATAGAATGACTTAATcc                                                              >  3:134148/1‑139 (MQ=255)

                                                                        cTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                         <  4:155634/127‑1 (MQ=255)

                                                                           gTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTa                                                          <  8:175733/139‑1 (MQ=255)

                                                                           gTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTAAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTa                                                          <  7:7331/139‑1 (MQ=255)

                                                                            tcaaCATTATCAAATTTTCGTTGATTTAATAGAAACATAGCGCTAAAATTAGCAGAAACTTAATACAACGCAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTAc                                                         <  4:114341/137‑1 (MQ=255)

                                                                              aaCCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGATTGACTTAATCCTGTACAg                                                       >  2:147672/1‑139 (MQ=255)

                                                                              aaCCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAg                                                       >  6:190638/1‑139 (MQ=255)

                                                                              aaCCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCAACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAg                                                       >  5:266882/1‑139 (MQ=255)

                                                                              aaCCTTATCAAATTTTCGTTGATTTAAAAGAAACATACCGGTAAAATTAGCTGTAACTAAATACCACGGAAATCAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAg                                                       <  5:222029/139‑1 (MQ=255)

                                                                              aaCCTAATAAAATTTTCGTTTATTAAATAGAAACAACGCGTAAAAATTACCAGTAAATTAATAGAACGTAAATCAAAAACGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAg                                                       <  1:119503/139‑1 (MQ=255)

                                                                                   tATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGTAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATa                                                  >  4:269234/1‑139 (MQ=255)

                                                                                   tATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCCTa                                                  >  7:142127/1‑139 (MQ=255)

                                                                                   tATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATa                                                  >  5:155961/1‑139 (MQ=255)

                                                                                    aTCAAATTTTCGTTGATTTAAAAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATTAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATa                                                  <  5:118819/138‑1 (MQ=255)

                                                                                     tCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATa                                                  >  4:218178/1‑137 (MQ=255)

                                                                                          ttttCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                              <  7:293146/72‑1 (MQ=255)

                                                                                           tttCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATc                                                               <  3:22732/118‑1 (MQ=255)

                                                                                            ttCTTTTATTTAATACAAACATAGCTTTAAAATTAGCTGTAACTTAATACAACGGAAATCAAAAACGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACtt                                          <  1:279495/138‑1 (MQ=255)

                                                                                            ttCGTTGCTTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTATTAGAACGTAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACtt                                          <  6:59994/138‑1 (MQ=255)

                                                                                            ttCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACtt                                          <  5:134530/138‑1 (MQ=255)

                                                                                             tCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTc                                         <  7:173934/138‑1 (MQ=255)

                                                                                               gTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAACGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGa                                       <  8:241329/138‑1 (MQ=255)

                                                                                                             aaCATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTAcc                                                                                        >  8:154229/1‑75 (MQ=255)

                                                                                                             aaCATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTg                                                            >  6:108964/1‑103 (MQ=255)

                                                                                                              aCATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGcc                        >  3:57081/1‑138 (MQ=255)

                                                                                                                 tAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACt                    <  8:133730/139‑1 (MQ=255)

                                                                                                                 tAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACt                    >  3:78052/1‑139 (MQ=255)

                                                                                                                   gCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACtt                   >  8:267561/1‑138 (MQ=255)

                                                                                                                       tAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTa                                                          <  8:109465/95‑1 (MQ=255)

                                                                                                                              aGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTAt       >  5:68532/1‑139 (MQ=255)

‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCC  >  pSIJ663_pHT315_araE_xy/1‑249

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`