BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	pSIJ663_pHT315_araE_xy	116	T→A	5.5%	intergenic (–/‑2862)	– / → eryR	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	pSIJ663_pHT315_araE_xy	116	0	T	A	5.5%	9244.9 / 76.6	3371	intergenic (–/‑2862)	–/eryR	–/–
Reads supporting (aligned to +/- strand): ref base T (1400/1734); new base A (5/231); total (1405/1966)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 3376 total aligned reads displayed.

‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGA‑TTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTT  >  pSIJ663_pHT315_araE_xy/1‑254

accctgataaatgcttcaataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCATATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGA‑TTTAATAGAAACATAGCGGTAAAATTAGCAGTAACtt                                                                                                                                            >  8:195161‑M1/23‑138 (MQ=255)

 ccctgataaatgcttcaataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGA‑TTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTa                                                                                                                                           <  7:223158‑M1/117‑1 (MQ=255)

      ataaatgcttcaataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGA‑TTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGaa                                                                                                                                     <  7:159863‑M1/123‑1 (MQ=255)

      ataaatgcttcaataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGA‑TTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGaa                                                                                                                                     <  4:100791‑M1/123‑1 (MQ=255)

      ataaatgcttcaataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGA‑TTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGaa                                                                                                                                     <  3:60707‑M1/123‑1 (MQ=255)

      ataaatgcttcaataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGA‑TTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGaa                                                                                                                                     <  5:287973‑M1/123‑1 (MQ=255)

      ataaatgcttcaataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGA‑TTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGaa                                                                                                                                     <  1:147313‑M1/123‑1 (MQ=255)

           tgcttcaataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGA‑TTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGa                                                                                                                                 <  7:141661‑M1/127‑1 (MQ=255)

           tgcttcaataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGA‑TTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGa                                                                                                                                 <  1:238053‑M1/127‑1 (MQ=255)

            gcttcaataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGATGA‑TTTAATAGAAACATAGCGGTAAATATAGCAGTAACTTAATAGAACGGaaa                                                                                                                               <  7:294365‑M1/129‑1 (MQ=255)

             cttcaataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGA‑TTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGaaa                                                                                                                               >  7:19156‑M1/10‑138 (MQ=255)

                caataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCATATTTTCGTTGA‑TTTAATAGAATCATAGCGGTAAAATTAGCTGTAACTTAATAGATCGGAAATGa                                                                                                                            <  3:30514‑M1/132‑1 (MQ=255)

                caataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGA‑TTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGa                                                                                                                            <  1:170397‑M1/132‑1 (MQ=255)

                caataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGA‑TTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGa                                                                                                                            <  5:244523‑M1/132‑1 (MQ=255)

                      tcctacctccAATGATGGAGAGTGACTTTTATTACTCAAATATTAATGTTTCTGGTCAACCTTATCAAATTTTCGTTTA‑TTTAATAGAAACAAAGCGGTAAATTTAGCATTAACTTAAAAGAACGCAATTGAAAAAAGc                                                                                                                     <  4:220859/134‑1 (MQ=255)

                      tCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGA‑TTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGc                                                                                                                     <  6:77849/139‑1 (MQ=255)

                      tCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGA‑TTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGc                                                                                                                     >  3:44301/1‑139 (MQ=255)

                        cATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGA‑TTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                                   >  8:215752/1‑139 (MQ=255)

                        cATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGA‑TTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                                   >  5:136023/1‑139 (MQ=255)

                        cATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGA‑TTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                                   <  5:8538/139‑1 (MQ=255)

                        cATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTATGGTGAACCTTATCAAATTTTCGTTGA‑TTTAATAGAAACATAGCGGTAAAATTAGCAGTAAGTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                                   >  5:308022/1‑139 (MQ=255)

                        cATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGAAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGA‑TTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATTAAAAAAGCCa                                                                                                                   <  3:253051/139‑1 (MQ=255)

                          tcctccAAATTTGGAGAGTGAGTTTTATGTCGAAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCTTTGA‑TTTAATAGAAACATAGCGGTAAAATTAGCAGTAAATTAATAGAACGGAAATGAAAAAAGCCAct                                                                                                                 <  7:51205/139‑1 (MQ=255)

                          tcctccAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGA‑TTTAATAGAAACATAGGGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                                   <  1:197596/137‑1 (MQ=255)

                          tcctccAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGA‑TTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGaa                                                                                                                                     >  4:50469/1‑119 (MQ=255)

                          tcctccAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGA‑TTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGTAAATGAAAAAAGCCAct                                                                                                                 <  2:18157/139‑1 (MQ=255)

                          tcctccAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGA‑TTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCAct                                                                                                                 >  7:126223/1‑139 (MQ=255)

                           cctccAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGA‑TTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCActc                                                                                                                >  2:229005/1‑139 (MQ=255)

                           cctccAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGA‑TTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCActc                                                                                                                >  8:173934/1‑139 (MQ=255)

                               cAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATAAAATTTTCGTTGA‑TTTAATAGAAACATAGCGTTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCat                                                                                                            <  8:62693/139‑1 (MQ=255)

                                aactttGGAGCGAGAGTTTAAGGGCGAAAAAATTAACGTTTCGCGTCACCCTTACAAAATTTTCGTTGA‑TTTAAAACAAACCTAGCGTAAAAATACCCAGAAACTAAAACCACCGCAAATCAAAAACGCCACTCTCata                                                                                                           <  2:276216/135‑1 (MQ=255)

                                    ttGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGA‑TTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAACGCCACTCTCATATGc                                                                                                        >  4:76715/1‑138 (MQ=255)

                                       gagaGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGA‑TTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTAtt                                                                                                    >  2:149617/1‑139 (MQ=255)

                                       gagaGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGA‑TTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTAtt                                                                                                    <  5:121018/139‑1 (MQ=255)

                                       gagaGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGA‑TTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTAtt                                                                                                    >  8:36332/1‑139 (MQ=255)

                                       gagaGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGA‑TTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTAtt                                                                                                    >  4:117487/1‑139 (MQ=255)

                                       gagaGTGAGTTTTATGTCGCAAAAATTAATGTTTCTGGTGAACATTATCAAATTTTCGTAGA‑TTTAATAGAAACATAGAGGTAATATTAGCAGTATCTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTAtt                                                                                                    >  1:50186/1‑139 (MQ=255)

                                        ttagtgagtTTTATGTCGAAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTTA‑TTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTg                                                                                                   <  8:241113/137‑1 (MQ=255)

                                            tgagtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGA‑TTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTa                                                                                               >  3:153378/1‑139 (MQ=255)

                                              agtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTTA‑TTAAAAAGAAACATAGCGGAAAAATTAGAAGTAACTTAATAGAACGCAAATGAAAAAAGCCACTCTCATATGCTATTGGCTAcc                                                                                             <  1:100421/139‑1 (MQ=255)

                                              agtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGA‑TTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCGCTCTCATATGCTATTGGCTAc                                                                                              >  6:242038/1‑138 (MQ=255)

                                              agtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGA‑TTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTAcc                                                                                             <  3:234018/139‑1 (MQ=255)

                                              agtTTTATGTCCAAAATATACATGTTTCTCGTGAACCTTACAAACTTTTCGTTGA‑TTTAATACAAACAACCCTTAAAAATTCCCATAAACTTAATACACCGGAAATCAAAAACGCCACTCTCATATGCTATTGGCTAcc                                                                                             <  5:20395/139‑1 (MQ=255)

                                                      gTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTGGA‑TTTAATAGAATCTTAGCGGTAAAATTAGCAGTAACTTAATATAACGGATATGATAAAAGACAGTCTCATATGATATTTGATAGCAAGCta                                                                                       >  8:81770/1‑136 (MQ=255)

                                                        cGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGA‑TTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAACAAAGCCACTCTCATATGCTATTGGCTACCAACCTGTAGc                                                                                   >  7:100885/1‑139 (MQ=255)

                                                          cAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGA‑TTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCGTATGCTATTGGCTACCAACCTTTAGc                                                                                   >  7:248680/1‑137 (MQ=255)

                                                          cAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGA‑TTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGc                                                                                   >  7:90774/1‑137 (MQ=255)

                                                          cAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGA‑TTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGc                                                                                   >  6:222467/1‑137 (MQ=255)

                                                          cAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGA‑TTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGc                                                                                   >  2:51417/1‑137 (MQ=255)

                                                             attttAATGTTTCTTGTGAACCTTATCAATTTTTGGTTGA‑TTTAATAGAAACATAGCGTTAAATTTAGCATTAACTTAATACAACGGAAATTAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                              <  2:255005/136‑1 (MQ=255)

                                                             atatTAATTTTTCTGGTGAACCTTATCAAATTTTCGTTTA‑TTTATTATAAACATAGTGGTAAATTTAGGAGTTACTTAATTGAACGGAAATGAAAAAAGCCACTCTCATTTGCTATTGGCTACCAACCTTTAGCGAGaa                                                                              <  7:72243/139‑1 (MQ=255)

                                                             atatTAATGTTTGTGGTGACCCTTATCAATTTTTCGTTTT‑TTTAATAGAAAAATAGCGGAAAATTTAGAATTAACTTAAAAGATCGGAAATTAAAAACGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                              <  8:294365/139‑1 (MQ=255)

                                                             atatTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGA‑TTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                              <  5:106065/139‑1 (MQ=255)

                                                             atatTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGA‑TTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                              <  6:143269/139‑1 (MQ=255)

                                                                  aaTTTTTCTGGCGACAGTAATAAAATTTTCGGTTT‑TTTAATTGAACCATTGGGTTAAAATACGCATAAACTTAATTGCCCGGAAATGAAAAACGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACt                                                                         <  4:134835/139‑1 (MQ=255)

                                                                   aTGTTTCTGGTCAACATAAAAAAATTATCGTTGA‑TTTAATAAAAACATAGGGGAAAAATTCCAATTAACTAAAAACACCGCAAATCAAAAACGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACt                                                                         <  4:159961/138‑1 (MQ=255)

                                                                    tGTTTCTGGTGAACCTTATCAAATTTTCGTTGA‑TTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTa                                                                       <  2:206212/139‑1 (MQ=255)

                                                                      tttCTGGTGAACCTTTTAAAATTTTCGTTGA‑TTAAATAGAAAAATAGCGTTAAAATTAGCAGTAACTAAATAGAACTGAAAGTAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTaa                                                                      <  6:164137/138‑1 (MQ=255)

                                                                       ttCTGGTGAACCTTATCAAATTTTCGTTGA‑TTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTCCCAACCTTTAGCGAGAATGACTTAATc                                                                    >  5:46393/1‑139 (MQ=255)

                                                                        tCTGGTGAACCTTATCAAATTTTCGTTGA‑TTTAATAGACACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATcc                                                                   >  3:121274/1‑139 (MQ=255)

                                                                        tCTGGTGAACCTTATCAAATTTTCGTTGA‑TTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCACCCTTTAGCGAGAATGCCTTAATcc                                                                   >  6:128264/1‑139 (MQ=255)

                                                                        tCTGGTGAACCTTATCAAATTTTCGTTGA‑TTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATcc                                                                   >  6:188715/1‑139 (MQ=255)

                                                                        tCTGGTGAACCTTATCAAATTTTCGTTGA‑TTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATcc                                                                   >  3:138661/1‑139 (MQ=255)

                                                                        tCTGGTGAACCTTATCAAATTTTCGTTGA‑TTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATcc                                                                   >  5:6611/1‑139 (MQ=255)

                                                                        tCTGGTGAACCTTATCAAATTTTCGTTGA‑TTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATcc                                                                   >  6:54394/1‑139 (MQ=255)

                                                                        tCTGGTGAACCTTATCAAATTTTCGTTGA‑TTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATcc                                                                   >  1:282360/1‑139 (MQ=255)

                                                                        tCTGGTGAACCTTATCAAATTTTCGTTGA‑TTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGACACTCTCATATGCTATTGGCTACCAGCCTTTAGCGAGAATGACTTAATc                                                                    >  3:81214/1‑138 (MQ=255)

                                                                         cTGGTGAACCTTATCAAATTTTCGTTGA‑TTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCt                                                                  >  8:153571/1‑139 (MQ=255)

                                                                         cTGGTGAACCTTATCAAATTTTCGTTGA‑TTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCt                                                                  >  8:133325/1‑139 (MQ=255)

                                                                            gTGAACCTTATAAATTTTTCTTTTA‑TTTAATAGAAACATAGCGGTAAAATTAGAAGTAAATTAATACAACGTAAATCAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTa                                                               <  5:178168/139‑1 (MQ=255)

                                                                             tGAACATTATAAAATTTTCGTTGATTTTAAAACAAACATAGCGGAAAAATTAGCAGAAACTTAATATAACGGAAATTAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTa                                                               <  1:97470/139‑1 (MQ=255)

                                                                               taCCTTATCAAATTTTCGTTGA‑TTTAATATAAACATAGCGGAAAAAATAGCAGTAAATAATAAGAACGTAAATCAAAAACGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAg                                                            <  5:222640/138‑1 (MQ=255)

                                                                               cacattATAAAATTTTCGTTGA‑TTTAATAGAAACATAGCGTTAAAATTAGCAGTAACTTAATACAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAg                                                            <  2:122677/135‑1 (MQ=255)

                                                                               aaCCTTATCAAATTTTCGTTGA‑TTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACt                                                                         >  7:17258/1‑126 (MQ=255)

                                                                               aaCCTTATCAAATATTCGTTCA‑TTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAACGCCACTCTCATATGCTATTGGCTACCAACCTTAAGCGAGAATGACTTAATCCTGTACAg                                                            >  1:260483/1‑139 (MQ=255)

                                                                               aaCCTTAAAAAATTTTCGTTTA‑TATAATAGAAACATAGCGTTAAAATTAGCAGTAACTAAATACAACGGAAATTAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAg                                                            <  3:176948/139‑1 (MQ=255)

                                                                               aaCCTAATAAAATTTTCGTTTA‑TTAAATAGAAACAACGCGTAAAAATTACCAGTAAATTAATAGAACGTAAATCAAAAACGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAg                                                            <  1:119503/139‑1 (MQ=255)

                                                                                aCCTTATCAAATTTTCCTTTA‑TTTAAAACAAACATAGCGGTAAAATTAGCAGTAAATTAATAGAACCGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAg                                                            <  3:276878/138‑1 (MQ=255)

                                                                                    tATCAAATTTTCGTTGA‑TTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATa                                                       >  5:267882/1‑139 (MQ=255)

                                                                                      tCAAATTTTCGTTTA‑TTTAATAGAAACATAGCGGTAAAATTCGCAGTAACTTAATAGACCGTAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCTAGAATGACTTAATCCTGTACAGCCATAc                                                      <  7:35430/138‑1 (MQ=255)

                                                                                      tCAAATTTTCGTTGA‑TTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATa                                                       >  6:108569/1‑137 (MQ=255)

                                                                                           ttttCGTTGA‑TTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACt                                                >  8:108568/1‑139 (MQ=255)

                                                                                             ttctctGA‑TTAAAAAGAAACATAGCGTTAAAAATAGCATTAACTTAATACAACGGAAAGGAAAAACGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACtt                                               <  6:271874/133‑1 (MQ=255)

                                                                                             ttCGTTGA‑TTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACtt                                               <  1:74365/138‑1 (MQ=255)

                                                                                             ttCGTTGA‑TTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACtt                                               <  4:188798/138‑1 (MQ=255)

                                                                                              tctttta‑TTTATTACAAACATCGCGTTAAAATTAGAACTAACTAAATATAACTGAAATCAAAAACGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTc                                              <  1:215864/132‑1 (MQ=255)

                                                                                              tCGTTGA‑TTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTc                                              >  1:307924/1‑138 (MQ=255)

                                                                                               cGTTGA‑TTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGa                                            >  3:106027/1‑139 (MQ=255)

                                                                                                        tAATATAAACATAGCTTAAAAATAAGCAGAAAATTAAAAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATaa                                     <  4:306278/138‑1 (MQ=255)

                                                                                                               aaCATAGCTGTAAAATTAGCATTAACTAAATACAACGTAAATGAAAACAGCCACTCTCATATGCTAATGGCTAGCAACCTTTAGCGATAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGcc                             <  5:156995/139‑1 (MQ=255)

                                                                                                                aCATAGCGGTAAAATTAGCAGTAACTTAATAGATCGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGcc                             >  3:210192/1‑138 (MQ=255)

                                                                                                                aCATAGAGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATAGGATACCAACATTTAGAGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGcc                             >  4:246971/1‑138 (MQ=255)

                                                                                                                     gCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACtt                        >  8:267561/1‑138 (MQ=255)

                                                                                                                      cGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTc                       >  2:77840/1‑138 (MQ=255)

                                                                                                                        gTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                              <  4:272443/81‑1 (MQ=255)

                                                                                                                           aaaTTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAAt                   >  7:203697/1‑137 (MQ=255)

                                                                                                                              ttAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTa                                                               <  6:289883/90‑1 (MQ=255)

                                                                                                                                aggaGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTAt            >  2:308785/4‑139 (MQ=255)

                                                                                                                                       aCTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACt                                                                         <  6:309118/71‑1 (MQ=255)

                                                                                                                                       aCTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTAAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCtt     >  7:275332/1‑139 (MQ=255)

‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGA‑TTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTT  >  pSIJ663_pHT315_araE_xy/1‑254

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`