BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	pSIJ663_pHT315_araE_xy	126	G→C	5.7%	intergenic (–/‑2852)	– / → eryR	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	pSIJ663_pHT315_araE_xy	126	0	G	C	5.7%	9006.9 / 71.6	3486	intergenic (–/‑2852)	–/eryR	–/–
Reads supporting (aligned to +/- strand): ref base G (1648/1335); new base C (4/242); total (1665/1821)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 3583 total aligned reads displayed.

‑‑‑‑‑‑‑‑‑‑TCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGAACA  >  pSIJ663_pHT315_araE_xy/1‑264

gcttcaataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGaaa                                                                                                                                         <  4:248564‑M1/129‑1 (MQ=255)

    caataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGa                                                                                                                                      <  7:29879‑M1/132‑1 (MQ=255)

    caataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGa                                                                                                                                      <  3:217192‑M1/132‑1 (MQ=255)

    caataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGAAGTAACTTAATAGAACGGAAATGa                                                                                                                                      <  7:181707‑M1/132‑1 (MQ=255)

         aTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGaaa                                                                                                                                         <  2:127961‑M1/129‑1 (MQ=255)

          tCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGc                                                                                                                               >  8:134454/1‑139 (MQ=255)

          tCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGc                                                                                                                               >  5:106164/1‑139 (MQ=255)

            cATCCTCCAATGTTGGAGTGTGAGTTTTATGTCGAAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTTATTTAATAGAAAAATAGCGGAAAAATTAGGAGTAACTTAATACACCGGAAATAAAAAAAGCCa                                                                                                                             <  7:152075/139‑1 (MQ=255)

            cATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATTAAAAAAGCCa                                                                                                                             <  3:111858/139‑1 (MQ=255)

            cATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAACGCCa                                                                                                                             <  3:66312/139‑1 (MQ=255)

            cATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                                             >  1:52065/1‑139 (MQ=255)

            cATCCTCCAAAGTGGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGAAACTTAATAGACCGGAAATGAAAAAAGCCa                                                                                                                             <  2:182769/139‑1 (MQ=255)

            cATCCTCAAATGTTGGAGAGTTTGTTTTATGTCGCAAATATTAATGTTTCTGGTTAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCTTTAACTTAATAGAACGCAAATTAAAAAAGCCa                                                                                                                             <  2:209896/139‑1 (MQ=255)

            cATCCACCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATAAAATTTTCGTTTATTAAATACAAAAATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATCAAAAAAGCCa                                                                                                                             <  8:155685/139‑1 (MQ=255)

              tcctccAATGTTGGAGTGTGAGTTTTATGTCGCAAATATTATTGTTTCTGGTTAACATTATCAAATTTTCGTTGATTTAATAGAAACATAGGGGTAAAATTAGCAGTAACTTAATTGAACGGAAATGAAAAAAGCCAct                                                                                                                           <  7:214952/139‑1 (MQ=255)

              tcctccAAAGTTGGTGATTGAGTTTTATGTCGAAAATATTAATGTTTCTGTTGAACTTTATCAAATTTTCTTTGATTTAATAGAAAAATAGCGTTAAAATTACCAGTAACTTAATAGAACGGAAATGAAAAAAGCCAct                                                                                                                           <  3:129165/139‑1 (MQ=255)

              tcctccAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGaaa                                                                                                                                    >  6:146200/1‑130 (MQ=255)

              tcctccAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCAct                                                                                                                           >  1:191995/1‑139 (MQ=255)

              tcctccAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCAct                                                                                                                           <  6:251754/139‑1 (MQ=255)

              tcctcaAAAGTTGTGTTGTGAGTTTTATGCCCAAAATATTATTGTTTCTGTTCAACATTATAAAATTTTCGTTGATTTAATATAAACATAGCGTAAAAATTACCATTAACTTAATCCACCGTAAATCAAAAACGCCAct                                                                                                                           <  6:36967/139‑1 (MQ=255)

               cctccAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                                             <  7:198612/136‑1 (MQ=255)

                   cAAAGTTTGATAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTTATTAAAAAGAAACATAGCGGAAAAATTAGCAGTAACTTAATAGAACGGAAATCAAAAAAGCCACtctc                                                                                                                        <  5:90882/137‑1 (MQ=255)

                   cAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCa                                                                                                                       >  4:52303/1‑138 (MQ=255)

                    aaaGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAAAATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCata                                                                                                                     <  3:31467/139‑1 (MQ=255)

                          ggAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCAACTCTCATATGatat                                                                                                               >  3:90045/1‑139 (MQ=255)

                           gagaGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGCACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAATTTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTAtt                                                                                                              >  3:48018/1‑139 (MQ=255)

                           gagaGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTAtt                                                                                                              >  3:284063/1‑139 (MQ=255)

                           gagaGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTAtt                                                                                                              >  6:171165/1‑139 (MQ=255)

                           gagaGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTAtt                                                                                                              >  6:800/1‑139 (MQ=255)

                                tgagtAATATGTCGCAAATATTAAAGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGAAACTTAATAGAACGGAAATGAAAAACGCCACTCTCATAACCTATTGGATa                                                                                                         >  8:3920/1‑139 (MQ=255)

                                  agtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTTATTAAAAAGAAACATAGCGGAAAAATTAGAAGTAACTTAATAGAACGCAAATGAAAAAAGCCACTCTCATATGCTATTGGCTAcc                                                                                                       <  1:100421/139‑1 (MQ=255)

                                  agtTTTATGCCCCAAAAATAAATGTTTCTGTTCAACCTTATCAAATTTTCGTTCATATAATACAAACAAAGCGGAAAAATAAGCAGAAACTTAAAACACCCCAAATCAAAAACGCCACTCTCATATGCTATTGGCTAcc                                                                                                       <  4:107064/139‑1 (MQ=255)

                                   gtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATAAAATTTTCGTTTATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTAcc                                                                                                       <  7:234274/138‑1 (MQ=255)

                                   gtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATAAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTAcc                                                                                                       <  8:84353/138‑1 (MQ=255)

                                      ttATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAAcc                                                                                                   >  3:240209/1‑139 (MQ=255)

                                       tATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCt                                                                                                  >  5:271292/1‑139 (MQ=255)

                                       tATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCt                                                                                                  >  5:70021/1‑139 (MQ=255)

                                       tATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCt                                                                                                  >  8:294418/1‑139 (MQ=255)

                                           tCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAg                                                                                              <  4:272782/139‑1 (MQ=255)

                                              cAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGc                                                                                             >  4:12506/1‑137 (MQ=255)

                                                 tttttAATTTTTCTGTTTATCATTATATAATTTTCGTTGTATAAAAATAACAATTGCGTTAAAATTTGCTTTAAATATTTAGTACGCATTTTAAATAAGCCACTCTCATATGCTATTGGCTACGAACCTTTAGCGAGaa                                                                                        <  8:217533/136‑1 (MQ=255)

                                                 attttAATTTTTCTGGGGAACCTTATCAAATTTTCGTTTATTTAATAGAAACATAGCTCTAAATATAGAAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAAACTTTAGCGAGaa                                                                                        <  5:135695/136‑1 (MQ=255)

                                                 atatTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                        <  6:129013/139‑1 (MQ=255)

                                                 atatTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                        <  5:50420/139‑1 (MQ=255)

                                                 atatTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                        <  5:244654/139‑1 (MQ=255)

                                                 atatTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                        <  2:92128/139‑1 (MQ=255)

                                                       aTGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACt                                                                                   <  1:28079/138‑1 (MQ=255)

                                                       aTGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGAAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACt                                                                                   <  1:76131/138‑1 (MQ=255)

                                                       aTGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAAAAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACt                                                                                   <  7:54131/138‑1 (MQ=255)

                                                         gTTTCTGGTGAACCTTATCATATTTTCGTTGATTTAATATAAACATTGCGGTAAAATTAGCAGTAACTTAATAGAACGTAAATGAAAAACGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTaa                                                                                <  7:3920/139‑1 (MQ=255)

                                                          tttCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTaa                                                                                <  2:41448/138‑1 (MQ=255)

                                                          tttCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTaa                                                                                <  8:64320/138‑1 (MQ=255)

                                                            tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                                             >  5:222193/1‑91 (MQ=255)

                                                            tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCATTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATcc                                                                             >  7:91591/1‑139 (MQ=255)

                                                            tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATg                                                                                                                   >  5:120514/1‑101 (MQ=255)

                                                            tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATcc                                                                             >  2:234586/1‑139 (MQ=255)

                                                            tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATcc                                                                             >  3:257125/1‑139 (MQ=255)

                                                            tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATc                                                                              <  3:146789/138‑1 (MQ=255)

                                                            tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCAACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATcc                                                                             >  5:201114/1‑139 (MQ=255)

                                                               ggTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTg                                                                           >  3:186677/1‑138 (MQ=255)

                                                                gTGACCCTTATCAAATTTTAGTTGATTTAATAGAAACATAGCGGTAAAATTAGCCGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTa                                                                         <  8:176042/139‑1 (MQ=255)

                                                                gTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTATCTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTa                                                                         <  6:110403/139‑1 (MQ=255)

                                                                gTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTa                                                                         <  7:256101/139‑1 (MQ=255)

                                                                 ttaaCATTATAAAATTTTCGTTTATTTAATACAAAAATAGCGTTAAAATTAGCATTAATTAAATACAAGTTAAATTAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTa                                                                         <  2:281586/136‑1 (MQ=255)

                                                                    actttATAAAATTTTCTTTTATTAAATACAAAAAAAGCTTAAAAATTAGCAGAAAATTAAAACACCGCAAATCAAAAACGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAg                                                                      <  4:167639/135‑1 (MQ=255)

                                                                    acattATAAAATTTTGGTTGATTTAATATAAACATAGCGTTAAAATTAGCAGTAAATTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAg                                                                      <  6:276662/135‑1 (MQ=255)

                                                                          tCAAATTTTCGTTGATTTAATAGAAACATAGCTGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGACATa                                                                 >  1:232409/1‑137 (MQ=255)

                                                                          tCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATa                                                                 >  8:110122/1‑137 (MQ=255)

                                                                          tCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATAc                                                                <  6:227281/138‑1 (MQ=255)

                                                                               ttttCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTa                                                                         <  2:258194/124‑1 (MQ=255)

                                                                                 ttCGTTTATTAAATACAAACATAGCGTTAAAATTACCAGTAACTAAATACAACGCAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACtt                                                         <  7:133589/138‑1 (MQ=255)

                                                                                  tCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTc                                                        >  1:117897/1‑138 (MQ=255)

                                                                                  tCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTc                                                        <  4:143239/138‑1 (MQ=255)

                                                                                           tAATATAAACATAGCTTAAAAATAAGCAGAAAATTAAAAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATaa                                               <  4:306278/138‑1 (MQ=255)

                                                                                                   aCATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGcc                                       >  6:195436/1‑138 (MQ=255)

                                                                                                       aGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAg                                                                      >  6:60750/1‑103 (MQ=255)

                                                                                                       aGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACtt                                                         <  8:19528/116‑1 (MQ=255)

                                                                                                       aGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACtt                                  >  2:65029/1‑139 (MQ=255)

                                                                                                       aGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACtt                                  >  3:162817/1‑139 (MQ=255)

                                                                                                        tcgttAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTAATAAGAGGCGCCAACTTc                                 <  7:28489/135‑1 (MQ=255)

                                                                                                           gTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCaaa                              >  5:29167/1‑139 (MQ=255)

                                                                                                                aTTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGt                         <  1:256331/139‑1 (MQ=255)

                                                                                                                   aGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCACCCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCCACTTCAAATAAGTTAt                      >  1:9989/1‑139 (MQ=255)

                                                                                                                   aGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTAt                      >  4:225844/1‑139 (MQ=255)

                                                                                                                          aCTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGTCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTAAAAGAGGCGCCAACTTCAAATAAGTTATTTGCCtt               >  5:155759/1‑139 (MQ=255)

                                                                                                                          aCTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACt                                                                                   >  5:309118/1‑71 (MQ=255)

                                                                                                                           cTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTg              <  4:197027/139‑1 (MQ=255)

                                                                                                                                  gAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTcgc       >  2:155290/1‑139 (MQ=255)

                                                                                                                                     cGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                        >  7:110035/1‑55 (MQ=255)

                                                                                                                                     cGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGaa    >  1:21222/1‑139 (MQ=255)

                                                                                                                                     cGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGaa    >  1:165209/1‑139 (MQ=255)

                                                                                                                                     cGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGaa    >  1:143372/1‑139 (MQ=255)

                                                                                                                                     cGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGaa    >  4:82325/1‑139 (MQ=255)

                                                                                                                                     cGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGaa    >  5:174547/1‑139 (MQ=255)

                                                                                                                                     cGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGaa    >  7:169103/1‑139 (MQ=255)

                                                                                                                                     cGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGaa    >  6:295969/1‑139 (MQ=255)

                                                                                                                                     cGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACATAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGAGCCAAAATCAAAAAATAAATATGACTTCTTTTCGCGaa    >  1:305874/1‑139 (MQ=255)

                                                                                                                                     cGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTGAGCGAGAATGACTTAATCCTGTACAGCCATACAGCACTTCGACTTATAAGAGTCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGa     >  7:76906/1‑138 (MQ=255)

                                                                                                                                      ggAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGaa    <  8:241527/138‑1 (MQ=255)

                                                                                                                                       gAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGAACa  <  8:268626/139‑1 (MQ=255)

‑‑‑‑‑‑‑‑‑‑TCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGAACA  >  pSIJ663_pHT315_araE_xy/1‑264

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`