BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	pSIJ663_pHT315_araE_xy	137	A→C	5.6%	intergenic (–/‑2841)	– / → eryR	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	pSIJ663_pHT315_araE_xy	137	0	A	C	5.6%	8648.8 / 91.1	3428	intergenic (–/‑2841)	–/eryR	–/–
Reads supporting (aligned to +/- strand): ref base A (1660/1494); new base C (18/250); total (1683/1745)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 3566 total aligned reads displayed.

TCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGAACAAGGCTTATTAG  >  pSIJ663_pHT315_araE_xy/1‑275

accctcctccAACGTTGGCTAGTTATTTTTATCTCGAAAATATTAATGTTTCTGGTGAACCTAATCAAATTTTCGTTGATTTAATACAAACATAGCGCTAAAATAAGCATTAACTTAATACACCGCAAATCAAAAAAGc                                                                                                                                          <  6:26023/135‑1 (MQ=255)

  catcatCCAAAGTTGGAGAGTGTGTTTTATTTCGAAAATATTATTTTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAAATAAATAGAACGGAAATCAAAAAAGCCa                                                                                                                                        <  6:83827/139‑1 (MQ=255)

  cATCCTCCAAAGTTTGAGAGTGAGTTTTAGGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTTATTTAATAGAAACATAGCGTTAAAATTAGCATTAACTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                                                        <  7:97023/139‑1 (MQ=255)

  cATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                                                        <  5:118373/139‑1 (MQ=255)

  cATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGCAAATGAAAAAAGCCa                                                                                                                                        <  2:7995/139‑1 (MQ=255)

  cATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTAAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATACAACGGAAATGAAAAAAGCCa                                                                                                                                        <  3:281570/139‑1 (MQ=255)

  cATCCTCCAAAGTGGGAGAGTGAGTTTTATGTCCCAAATATAAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                                                        <  5:173391/139‑1 (MQ=255)

    tcctccAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGTAAATGAAAAAAGCCAct                                                                                                                                      <  2:18157/139‑1 (MQ=255)

    tcctccAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCAct                                                                                                                                      <  5:134059/139‑1 (MQ=255)

    tcctccAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCAct                                                                                                                                      >  1:278618/1‑139 (MQ=255)

    tcctccAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCAct                                                                                                                                      >  4:16975/1‑139 (MQ=255)

    tcctccAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATAAGCAGTAACTTAATAGAACGGAAATGAAATAATCCAc                                                                                                                                       >  6:44805/1‑138 (MQ=255)

    tcctccAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTCTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCAct                                                                                                                                      <  5:273265/139‑1 (MQ=255)

    tcctccAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATATTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTAAATAGAACGTAAATGAAAAAAgaaact                                                                                                                                      >  8:270343/1‑134 (MQ=255)

     cctccAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCActc                                                                                                                                     >  4:1267/1‑139 (MQ=255)

         cAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCa                                                                                                                                  >  8:197683/1‑138 (MQ=255)

          aaaGTTTGATAGTGAGTTTTATGTCGCAATTATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATTAAAAAAGCCACTCTCata                                                                                                                                <  6:301310/139‑1 (MQ=255)

              ttGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGc                                                                                                                             >  8:117315/1‑138 (MQ=255)

                ggAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCAACTCTCATATGatat                                                                                                                          >  3:90045/1‑139 (MQ=255)

                 gagaGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTAtt                                                                                                                         >  5:9854/1‑139 (MQ=255)

                 gagaGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTAtt                                                                                                                         <  7:20066/139‑1 (MQ=255)

                 gagaGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTAtt                                                                                                                         >  4:306517/1‑139 (MQ=255)

                 gagaGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTAtt                                                                                                                         >  1:71618/1‑139 (MQ=255)

                 gagaGTGAGTTTTATGACGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAAAGAAAAAAGCCACTCTCATATCCTAtt                                                                                                                         >  8:179626/1‑139 (MQ=255)

                      tgagtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                                                        >  2:5632/1‑119 (MQ=255)

                      tgagtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTAtt                                                                                                                         <  7:1521/134‑1 (MQ=255)

                      tgagtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTa                                                                                                                    >  8:84163/1‑139 (MQ=255)

                      tgagtAATATGTCGCAAATATTAAAGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGAAACTTAATAGAACGGAAATGAAAAACGCCACTCTCATAACCTATTGGATa                                                                                                                    >  8:3920/1‑139 (MQ=255)

                        agtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTTATTAAAAAGAAACATAGCGGAAAAATTAGAAGTAACTTAATAGAACGCAAATGAAAAAAGCCACTCTCATATGCTATTGGCTAcc                                                                                                                  <  1:100421/139‑1 (MQ=255)

                        agtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTAcc                                                                                                                  <  7:286579/139‑1 (MQ=255)

                        agtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTAc                                                                                                                   >  3:30935/1‑138 (MQ=255)

                        agtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTAc                                                                                                                   >  3:14301/1‑138 (MQ=255)

                        agtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTAc                                                                                                                   >  7:182273/1‑138 (MQ=255)

                        agtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATAAAATTTTCGTTGATTTAATATAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATT‑GCTAcc                                                                                                                  <  1:95943/138‑1 (MQ=255)

                         gtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGACCGTAAATGAAAAACGCCACTCTCATATGCTATTGGCTAcc                                                                                                                  <  1:49778/138‑1 (MQ=255)

                                   gCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGc                                                                                                        <  2:204340/138‑1 (MQ=255)

                                    cAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGc                                                                                                        >  6:282467/1‑137 (MQ=255)

                                    cAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGc                                                                                                        >  7:133730/1‑137 (MQ=255)

                                       atatTAATGTTTCTGTTGAACATTATCAAATTTTCTTTGATTTAATAGAAACATTGCGTTAAAATTAGCAGTAACTTAATACAACGCAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                                   <  1:208461/139‑1 (MQ=255)

                                       atatTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                                   <  8:162595/139‑1 (MQ=255)

                                       atatTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                                   <  8:218578/139‑1 (MQ=255)

                                       atatTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                                   <  7:81327/139‑1 (MQ=255)

                                       atatTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGAAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                                   <  3:236717/139‑1 (MQ=255)

                                       atatTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTAAATAGAACCATACGGTTAAAATTAGCAGTAACTTAATAGACCGGAATTGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                                   <  3:46753/139‑1 (MQ=255)

                                       atatTAATGTTTCTGGTGAACCTTATAAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGCAAATGAAAAACGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                                   <  5:103672/139‑1 (MQ=255)

                                             ttGTTTATTGTTAATCTTATAATATTTTCTTTTTTTTATAACAAAAATTGCGTTAAAATTAGCATTATATAAAAACATCGAATTACAAAAACGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACt                                                                                              <  6:182695/137‑1 (MQ=255)

                                                tttCTGGTCAACCTTATCAAATTTTCGTTCAATTAAACCAAACATAGCGGAAAAATTCGCAGTAACTAAATAGAACGCAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTaa                                                                                           <  1:304778/138‑1 (MQ=255)

                                                 tgggggTGAACGTGATGAAATTTTCGTTGTTTTATATGAACGATTGCGTTAAATTTAGCAGTAACTTAATCTCCCGTAAATAAAAAACGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATc                                                                                         <  2:151614/135‑1 (MQ=255)

                                                  tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCat                                                                                                                                 <  1:188369/98‑1 (MQ=255)

                                                  tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                                   <  1:250838/128‑1 (MQ=255)

                                                  tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATcc                                                                                        >  3:157520/1‑139 (MQ=255)

                                                  tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATcc                                                                                        >  3:124305/1‑139 (MQ=255)

                                                  tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCAACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATcc                                                                                        >  5:201114/1‑139 (MQ=255)

                                                   cTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                                                        <  2:30019/90‑1 (MQ=255)

                                                   cTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCt                                                                                       >  2:252558/1‑139 (MQ=255)

                                                   cTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCt                                                                                       >  7:57895/1‑139 (MQ=255)

                                                   cTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCt                                                                                       >  3:213694/1‑139 (MQ=255)

                                                      gTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACTTAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATTACTTAATccctg                                                                                     >  1:227163/1‑135 (MQ=255)

                                                       gtaaacTTATCAAATTTTCTTTGATTAAATATAAAATTAGCGGTAATATTTGCTTTAACTAAATAGAACGTAAATCAAAAAAGCCACTCTCATATGCTATTTGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTa                                                                                    <  3:107353/133‑1 (MQ=255)

                                                         aaatttATAAAATTTTCTTTGATTTAATACAAAAATGGCGCTAAAATAAGCAGAATATTAAAAGAAGGTAAATTAAAATAGCAACTCTCATATGCTATTGGCTACCAACCTATAGCGAGAATGTCTTAATCCTGTACAg                                                                                 <  7:4234/135‑1 (MQ=255)

                                                         aaCCTTATCAAATTTACGTTGATTAAATACAAACATAGCGGTAAAATTAGCCGTAACTAAATACAACGGAATTGAAAAACGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAg                                                                                 <  7:219306/139‑1 (MQ=255)

                                                         aaCCTTATAAATTTTTCGTTTATTTAATAGAAACATCGCGGTAAAATTCGCCGAAACTTAATAGAACCCAAATCAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAg                                                                                 <  4:248687/139‑1 (MQ=255)

                                                           ccatataAAATTTTCGTTGATATAATAGAAACATAGCGGTAAAATTAGAAGTAACTTAATAGAACGGAAATGAAAAAAGCCAATCTCATATGCTATTGTCTACCACAATGTATCGAGAATGACTTAATCCTCTActga                                                                                >  6:158957/4‑135 (MQ=255)

                                                           ccTTATCAAATTTTCGTTTATTTAATCGACACTTAGCGGTAAAATTTGCAGAATCTTAATTGAAGGGAAATCAAAAAAGCCAGTCTCATATGCTATTGGCTACCAAGCTTTAGCGAGAATGACTTAATCCTGTACAGcc                                                                               <  4:145322/139‑1 (MQ=255)

                                                                acAAATTTTCGTTTATTAAATATAACCATAGCTTTAAAATTACAATTAAATAAAAACAACGCAAATGAAAAACGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATAc                                                                           <  3:182679/137‑1 (MQ=255)

                                                                      tttCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATc                                                                                         >  4:22732/1‑118 (MQ=255)

                                                                       ttCTTTTATTTAATATAAACATAGCGGAAAAATTAGCCTTAAATTAATACACCGAAAATCAAAAACGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACtt                                                                    <  5:33958/138‑1 (MQ=255)

                                                                       ttCGTTGATTTAATACAAACATAGCGGAAAAATTAGCAGTAACTTAATAGACCGGAAATGAAAAACGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACtt                                                                    <  6:232465/138‑1 (MQ=255)

                                                                        tCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAACGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTc                                                                   <  4:51329/138‑1 (MQ=255)

                                                                        tCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTc                                                                   >  1:117897/1‑138 (MQ=255)

                                                                                        aaCATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGAGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGgcgc                                                   >  3:256740/1‑138 (MQ=255)

                                                                                         aCATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGcc                                                  >  6:6510/1‑138 (MQ=255)

                                                                                         aCATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGcc                                                  >  1:161464/1‑138 (MQ=255)

                                                                                             aGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACtt                                             >  6:210762/1‑139 (MQ=255)

                                                                                                 gTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                                   <  4:272443/81‑1 (MQ=255)

                                                                                                   aaaaTTAGCAGAAACATAATATAACGCAAATGAAAAACGCCACTCTCATATGCTATAGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATCAGAGTCGCCAACTTCAAAt                                        <  4:198261/138‑1 (MQ=255)

                                                                                                    aaaTTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGgcgc                                                   >  2:22690/1‑126 (MQ=255)

                                                                                                    aaaTTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATa                                       <  7:261724/138‑1 (MQ=255)

                                                                                                            aGTAACTTTATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACAAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTg                              >  5:221111/1‑139 (MQ=255)

                                                                                                                 cTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCATGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTg                         >  7:236902/1‑139 (MQ=255)

                                                                                                                      tAGAACGTAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTc                    <  8:138340/139‑1 (MQ=255)

                                                                                                                         aaCGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTaa                                                                                           <  6:134046/65‑1 (MQ=255)

                                                                                                                           cgtaaaTGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGaa               <  7:303469/136‑1 (MQ=255)

                                                                                                                           cGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAAc                                               <  4:78160/107‑1 (MQ=255)

                                                                                                                           cGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGaa               >  4:107945/1‑139 (MQ=255)

                                                                                                                           cGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGaa               >  7:169103/1‑139 (MQ=255)

                                                                                                                           cGGAAATGAAAAAAGCCACTCTCATATGCTATAGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCTACAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGaa               >  3:6454/1‑139 (MQ=255)

                                                                                                                            gtaaaTGAAAACCGCCACTCTCATCTGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATATGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGAAc              <  5:154728/137‑1 (MQ=255)

                                                                                                                            ggAAATTAAAAACGCCACTCTCATATGCTATTGGCTACCTACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGAAc              <  2:123043/139‑1 (MQ=255)

                                                                                                                             gAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGAACa             <  5:295969/139‑1 (MQ=255)

                                                                                                                             caaaTCAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGCATGACTTAATCCTGTACAGCCATACAGTACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGAACa             <  5:246172/138‑1 (MQ=255)

                                                                                                                              aaaTGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATa                                                                            <  8:62975/75‑1 (MQ=255)

                                                                                                                              aaaTGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGAAc              >  2:272314/1‑137 (MQ=255)

                                                                                                                                aTGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGAACAAgg          <  8:2426/139‑1 (MQ=255)

                                                                                                                                 tGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGAACAAGGc         <  5:56340/139‑1 (MQ=255)

                                                                                                                                    aaaaaGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGAACAAGGCtta      <  8:295047/139‑1 (MQ=255)

                                                                                                                                    aaaaaGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGAACAAGGCtt       <  6:116541/138‑1 (MQ=255)

                                                                                                                                     aaaaGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGAACAAGGCttat     <  5:76029/139‑1 (MQ=255)

                                                                                                                                     aaaaGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGAACAAGGCttat     <  7:110122/139‑1 (MQ=255)

                                                                                                                                        aGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGAACAAGGCttat     >  1:1424/1‑136 (MQ=255)

TCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGAACAAGGCTTATTAG  >  pSIJ663_pHT315_araE_xy/1‑275

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`