BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	pSIJ663_pHT315_araE_xy	3,783	G→T	5.1%	intergenic (+68/‑88)	eryR → / → ampR	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	pSIJ663_pHT315_araE_xy	3,783	0	G	T	5.1%	7586.4 / 54.7	2369	intergenic (+68/‑88)	eryR/ampR	–/–
Reads supporting (aligned to +/- strand): ref base G (699/1475); new base T (148/31); total (857/1512)
Fisher's exact test for biased strand distribution p-value = 1.09e-40
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 2435 total aligned reads displayed.

TAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATA‑TACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGTATCGGGGTACATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCC  >  pSIJ663_pHT315_araE_xy/3645‑3925

 aaGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAg                                                                                                                                                 <  4:247485/138‑1 (MQ=255)

 aaGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAg                                                                                                                                                 <  8:184847/138‑1 (MQ=255)

  aGTACCATTACTTATGAGGAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACTGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGat                                                                                                                                               <  2:139772/139‑1 (MQ=255)

  aGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGat                                                                                                                                               <  4:18189/139‑1 (MQ=255)

  aGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGat                                                                                                                                               <  3:14265/139‑1 (MQ=255)

  aGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGat                                                                                                                                               <  7:275519/139‑1 (MQ=255)

   gTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGata                                                                                                                                              <  1:120641/139‑1 (MQ=255)

   gTACCATTACTTATGAGCAAGTATTGTCTATTTATAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTCTGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGata                                                                                                                                              <  3:155903/139‑1 (MQ=255)

      ccATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTTAATTTTGAAAGTTAAACGTTACTAAATGGAATGGAGATAAATTATtatata‑taa                                                                                                                                          >  8:296264/1‑138 (MQ=255)

         ttACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTTATTTTGGGAAGTTAGACGGTTGTAAAGGGGATGGTGGTAATTTTTTTGAtt‑tagtgc                                                                                                                                       >  6:60817/1‑135 (MQ=255)

         ttACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GGTTTTTTATTTTGTAAGTTTAACGGTAGTAAAGGGAATGGTGATAAATTATTTTata‑ttatac                                                                                                                                       >  6:148118/1‑134 (MQ=255)

               aTGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATA‑TACTACTGAc                                                                                                                                   <  5:173383/137‑1 (MQ=255)

               aTGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATA‑TACTACTGAc                                                                                                                                   <  4:264131/137‑1 (MQ=255)

               aTGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATA‑TACTACTGAc                                                                                                                                   <  1:44781/137‑1 (MQ=255)

                 gAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTTAATTTTTAAAGTTAAACGTTACTTAAGGGAATGGAGATAAATTATTATTTA‑TAATAATGACAGCt                                                                                                                               >  7:164312/1‑139 (MQ=255)

                     aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATA‑TACTACTGACAGCTTCCa                                                                                                                           <  7:19787/139‑1 (MQ=255)

                       gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATA‑TACTACTGACAGCTTCCaag                                                                                                                         <  8:253125/139‑1 (MQ=255)

                       gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATA‑TACTACTGACAGCTTCCaag                                                                                                                         <  1:146949/139‑1 (MQ=255)

                       gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATA‑TACTACTGACAGCTTCCaag                                                                                                                         <  7:179296/139‑1 (MQ=255)

                       gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATA‑TACTACTGACAGCTTCCaag                                                                                                                         <  1:64748/139‑1 (MQ=255)

                        tATTTTCTATTTTTTATAGTTATCTATTATTTAACGTGATGAAATAATTCTATGAGTAGC‑TTTTTTATATTTGGAAAGTTACACGTTACTAAAGGTAATGGAGATAAATTATTAGATA‑TACTACTGACAGCTTCCaag                                                                                                                         <  3:54422/138‑1 (MQ=255)

                         aTTGTCTATTTTTAATAGTTATCTATTATTTAACGGGATGAAATAATTCTATGAGTCTC‑TTTTTTAAATTTGTAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATA‑TACTACTGACAGCTTCCaaga                                                                                                                        <  7:236249/138‑1 (MQ=255)

                               tATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATA‑TACTACTGACAGCTTCCAAGAAGCTaaa                                                                                                                 <  2:161896/139‑1 (MQ=255)

                                 tttttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATA‑TACTACTGACAGCTTCCAAGAAGCTaaa                                                                                                                 <  7:293448/137‑1 (MQ=255)

                                  ttttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTTAATTTTGTTAGTTAAAAGTTTATAAAAGGTATTGAGATAAAATTTTATATATTAATA‑TTACAACTTCAAAGAAACTaaatat                                                                                                              >  8:60515/1‑136 (MQ=255)

                                  ttttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GGTTTTTAATTTTGTAAATTACACCGTACTAAATGGGATTGAGTTAAATTATTAGATA‑TAATAATGACACCTGCCCAAAAGCTAAagag                                                                                                              >  6:81869/1‑139 (MQ=255)

                                   tttAATAGTTATCTATTATTTAACTGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGCAAAGATACACGTTACTAAAGGGACTGGAGATAAATTATTAGATA‑TACTACTGACAGCTTCCAAGAAGCTAAagag                                                                                                              <  3:117129/138‑1 (MQ=255)

                                   tttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATA‑TACTACTGACAGCTTCCAAGAAGCTAAagag                                                                                                              <  7:68907/138‑1 (MQ=255)

                                   tttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATA‑TACTACTGACAGCTTCCAAGAAGCTAAagag                                                                                                              <  7:219118/138‑1 (MQ=255)

                                            tATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTAAATTTTGAAAGTTAACCGTTTCTAAATGGAATGGAGATAAATTATTTGATA‑TACTAATGACAGCGTCCAACAAGCTAAAGAGGTACCTAtcg                                                                                                    >  7:48558/1‑139 (MQ=255)

                                                    attTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATA‑TACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACgg                                                                                             <  8:276784/138‑1 (MQ=255)

                                                    aattAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATA‑TACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACgg                                                                                             <  6:234856/136‑1 (MQ=255)

                                                     tttAACGGGAGGAAATAATTCTATGAGTCGCGTTTTTTAAATTTGAAAGTTTAAACG‑TAATAAATCGAAATGAGATAAAATAATATATA‑TACTAATGACAGCTTCACATAAGCTAAAAAGGTCCCAAGCGCCGAAgggg                                                                                           >  8:166719/1‑139 (MQ=255)

                                                           gggAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTATATA‑TACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTG                                                                                     <  6:109532‑M1/139‑1 (MQ=255)

                                                             ccggaaaTAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGATACACGTTACTAAAGGCAAGGGAGATAAATTATTAGATA‑TACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgg                                                                                   <  4:123495‑M1/137‑3 (MQ=255)

                                                              agtaaataaTTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATA‑TACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGggg                                                                                  <  3:29353‑M1/136‑4 (MQ=255)

                                                              aggaAATAATTCTATGAGCCGC‑TTTTTAAAATTTGGAAAGCTACACGGTACAAACGGGAATCGAGATAAATTATTAGATA‑TACTACTGACAGCTTCCACGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGggg                                                                                  <  2:84891‑M1/139‑4 (MQ=255)

                                                                  aataatTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGTAATGGAGATAAATTATTAGATA‑TACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtaca                                                                              <  4:149234‑M1/139‑8 (MQ=255)

                                                                  aataatTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATA‑TACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtaca                                                                              <  5:283650‑M1/139‑8 (MQ=255)

                                                                  aataatTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATA‑TACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtaca                                                                              <  1:259593‑M1/139‑8 (MQ=255)

                                                                  aataaaTCTATGATTCGC‑TTTTTTAAATATGGAAAGTTACACGTTACTAAAGGGAATGCAGATAAATTATTAGATA‑TACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtaca                                                                              <  2:68036‑M1/139‑8 (MQ=255)

                                                                    taatTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATA‑TACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacatt                                                                            <  5:129756‑M1/139‑10 (MQ=255)

                                                                    taatTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATA‑TACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacatt                                                                            <  2:294149‑M1/139‑10 (MQ=255)

                                                                         cTATGATCCGC‑GTTTTTAAATTTGGAAAGTAACAAGTTAATAAAGGGAATGTAGATAAATAATTAGATA‑TACAACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaa                                                                        <  5:44851‑M1/138‑14 (MQ=255)

                                                                         cTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATA‑TACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaa                                                                        <  3:73237‑M1/138‑14 (MQ=255)

                                                                            tGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATA‑TACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                                    <  4:204105‑M1/139‑18 (MQ=255)

                                                                            tGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATA‑TACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                                    <  3:254329‑M1/139‑18 (MQ=255)

                                                                            tGAGTCGC‑TTTTTTAAATTTGGAAAGATACACGTTACTAAAGGGAATGGAGATAAATTATTAGATA‑TACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                                    <  6:131237‑M1/139‑18 (MQ=255)

                                                                            tGAGTCGC‑TTTTTTAAATTTGGAAAGATACACGTTACTAAAGGGAATGGAGATAAATTATTAGATA‑TACTACTGACAGCTTCCAAGAAGCTAAAGAGGGCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                                    <  4:298800‑M1/139‑18 (MQ=255)

                                                                             gAGTCTA‑TTTTTTATATTTGGATAGTTACAAGTTACTAAAGGGAATTGAGATAAATTATTAGATA‑TACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                                    <  7:308925‑M1/138‑18 (MQ=255)

                                                                             gAGTCGC‑TTTTTTTAATTTTGAAATTTACACGTTACTAAAGTGAATGGAGATAAATTATTAGATA‑TACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacatttaaaaagg                                                                    <  7:143875‑M1/138‑18 (MQ=255)

                                                                             gAGTCGC‑TTTTTTTAATTTGGAAAGTTACACGTTAATAAAGGGAATGGAGATAAATTATTAGATA‑TACTACTTACAGCTTCCAAGCAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                                    <  5:30363‑M1/138‑18 (MQ=255)

                                                                             gAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATA‑TACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                                    <  1:101052‑M1/138‑18 (MQ=255)

                                                                              cgTCGA‑TTATTTAAATTTAGAAAGCTACACGTAACAAACGTCAATGTACATAAATTATTAGATA‑CACTCCTGACTGCTGCCACCAAGCAAAAGAGGCCCCTCGCGCCTACGGGGAATTT‑‑‑‑‑GGGGTACATTGAAAAAGGaa                                                             <  6:31684/138‑1 (MQ=255)

                                                                                  gC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATA‑TACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagt                                                              <  8:215047‑M1/139‑24 (MQ=255)

                                                                                      tttttaaatttttaaaGTTAGACGTTACTAAAGGGAATGGAGATAAATTATTAGAAA‑TACTACTGACAGCTTCCAAGAAGCTAAAGAGGGCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                                                            <  5:110434‑M1/138‑26 (MQ=255)

                                                                                       ttttAAATTTGGAAATTAACACTTTACTAAAGGGAATGGAGATAAATTATTATATA‑TACTACTGTCAGCTTCCAAGAAGCAAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                                                            <  8:132177‑M1/137‑26 (MQ=255)

                                                                                            aaTTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATA‑TACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtatt                                                     <  6:174505‑M1/139‑33 (MQ=255)

                                                                                              tttGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATA‑TACTACTGACAGCTTCCAAGAAGCTAAGGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattca                                                   <  4:226611‑M1/139‑35 (MQ=255)

                                                                                                   aaaGTTACACGTTACTAAAGGTAATGGAGATAAATTATTAGATA‑TACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatt                                              <  1:299170‑M1/139‑40 (MQ=255)

                                                                                                          cacGTTACTTTAGGGAATGGAGATAAATTATTAGTTA‑TACTACAGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgt                                       <  1:97209‑M1/139‑47 (MQ=255)

                                                                                                          cacGTTACTAAAGGTAATGGAGATAAATTATTAGATA‑TACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgt                                       <  3:32386‑M1/139‑47 (MQ=255)

                                                                                                           acGTTACTAAAGGGAATGGAGATAAATTATTAGATA‑TACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtc                                      <  7:256009‑M1/139‑48 (MQ=255)

                                                                                                            cGTTACTAAATGGAATGGAGATAAATTATTAGATA‑TACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGTGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcg                                     <  7:84574‑M1/139‑49 (MQ=255)

                                                                                                                aCTAAAGGGAATGGAGATAAATTATTAGATA‑TACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaatggaagagtaggagtattcaacatttccgtgtcgccct                                 >  2:42208‑M1/1‑87 (MQ=255)

                                                                                                                 cTAAAGGGAATGGAGATAAATTATTAGATA‑TACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgt                                       >  8:234403‑M1/1‑86 (MQ=255)

                                                                                                                    aaGGGAATGGAGATAAATTATTAGATA‑TACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                                                            <  5:229825‑M1/108‑26 (MQ=255)

                                                                                                                       ggAATGGAGATAAATTATTAGATA‑TACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAAt                                                                                        <  7:109197/77‑1 (MQ=255)

                                                                                                                       ggAATGGAGATAAATTATTAGATA‑TACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattccc                          >  5:257960‑M1/1‑80 (MQ=255)

                                                                                                                       ggAATGGAGATAAATTATTAGATA‑TACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgaggattcaacatttccgtgt                                       <  7:12667‑M1/126‑47 (MQ=255)

                                                                                                                         aaTGGAGATAAATTATTAGATA‑TACTACTGACAGCTTCCAAGAAGCTAAAGCGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattccctt                        >  6:192859‑M1/1‑78 (MQ=255)

                                                                                                                         aaTGGAGATAAATTATTAGATA‑TACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtctcccttattcactt                        >  5:201846‑M1/1‑78 (MQ=255)

                                                                                                                         aaTGGAGATAAATTATTAGATA‑TACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcgcttattccctt                        >  4:57029‑M1/1‑78 (MQ=255)

                                                                                                                         aaTGGAGATAAATTATTAGATA‑TACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattccctt                        >  1:83754‑M1/1‑78 (MQ=255)

                                                                                                                         aaTGGAGATAAATTATTAGATA‑TACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattccctt                        >  1:276115‑M1/1‑78 (MQ=255)

                                                                                                                         aaTGGAGATAAATTATTAGATA‑TACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattccctt                        >  6:290081‑M1/1‑78 (MQ=255)

                                                                                                                         aaTGGAGATAAATTATTAGATA‑TACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattccctt                        >  3:271835‑M1/1‑78 (MQ=255)

                                                                                                                         aaTGGAGATAAATTATTAGATA‑TACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattccctt                        >  5:87440‑M1/1‑78 (MQ=255)

                                                                                                                         aaTGGAGATAAATTATTAGATA‑TACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattccctt                        >  3:209519‑M1/1‑78 (MQ=255)

                                                                                                                         aaTGGAGATAAATTATTAGATA‑TACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattccctt                        >  3:191863‑M1/1‑78 (MQ=255)

                                                                                                                         aaTGGAGATAAATTATTAGATA‑TACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcg                                     >  2:210814‑M1/1‑78 (MQ=255)

                                                                                                                         aaTGGAGATAAATTATTAGATA‑TACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                                                            >  8:33152‑M1/1‑78 (MQ=255)

                                                                                                                         aaTGGAGATAAATTATTAGATA‑TACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa                                                                  <  1:168642‑M1/97‑20 (MQ=255)

                                                                                                                         aaTGGAGATAAATTATTAGATA‑TACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgcgtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattccctt                        >  1:216603‑M1/1‑78 (MQ=255)

                                                                                                                           tGGAGATAAATTATTAGATA‑TACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattccctttt                      >  2:265244‑M1/1‑76 (MQ=255)

                                                                                                                           tGGAGATAAATTATTAGATA‑TACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattccctttt                      >  5:62826‑M1/1‑76 (MQ=255)

                                                                                                                           tGGAGATAAATTATTAGATA‑TACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgccattattccctttt                      >  4:89761‑M1/1‑76 (MQ=255)

                                                                                                                           tGGAGATAAATTATTAGATA‑TACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcg                                     <  6:264864‑M1/124‑49 (MQ=255)

                                                                                                                              agaTAAATTATTAGATA‑TACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAAt                                                                                        >  2:125859/1‑70 (MQ=255)

                                                                                                                                aTAAATTATTAGATA‑TACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtaca                                                                              >  3:98086‑M1/1‑71 (MQ=255)

                                                                                                                                  aaaTTATTAGATA‑TACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattga                                                                          <  1:194883‑M1/80‑12 (MQ=255)

                                                                                                                                   aaTTATTAGATA‑TACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggca              >  3:170985‑M1/1‑68 (MQ=255)

                                                                                                                                   aaTTATTAGATA‑TACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggca              >  2:184390‑M1/1‑68 (MQ=255)

                                                                                                                                   aaTTATTAGATA‑TACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggca              >  5:144881‑M1/1‑68 (MQ=255)

                                                                                                                                       attaGAAA‑AACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGATTTTGgggtacattgaaaaaggaagagtatgagtattcaacattaccgtgtcgcgcttaatccctattttgcggctttat          >  8:152724‑M1/1‑64 (MQ=255)

                                                                                                                                        ttaGATA‑TACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttg         >  2:242651‑M1/1‑63 (MQ=255)

                                                                                                                                        ttaGATA‑TACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttg         >  3:228516‑M1/1‑63 (MQ=255)

                                                                                                                                        ttaGATA‑TACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                                    >  8:105726‑M1/1‑63 (MQ=255)

                                                                                                                                         taGATA‑TACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgc        >  8:74609‑M1/1‑62 (MQ=255)

                                                                                                                                          aGATA‑TACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgcc       >  1:23616‑M1/1‑61 (MQ=255)

TAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATA‑TACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGTATCGGGGTACATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCC  >  pSIJ663_pHT315_araE_xy/3645‑3925

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`