BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	pSIJ663_pHT315_araE_xy	5,173	T→G	6.4%	intergenic (+442/–)	ampR → / –	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	pSIJ663_pHT315_araE_xy	5,173	0	T	G	6.4%	7927.9 / 126.0	4023	intergenic (+442/–)	ampR/–	–/–
Reads supporting (aligned to +/- strand): ref base T (1831/1831); new base G (215/120); total (2054/1969)
Fisher's exact test for biased strand distribution p-value = 6.13e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 4153 total aligned reads displayed.

ATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGT‑GTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGC  >  pSIJ663_pHT315_araE_xy/5035‑5311

      aaTACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCACGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGT‑GTCTTACCGGGTTGGACt                                                                                                                                      <  6:55459/139‑1 (MQ=255)

           tGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGT‑GTCTTACCGGGTTGGACTCAAGa                                                                                                                                 >  5:309908/1‑139 (MQ=255)

            gttcTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGT‑GTCTTACCGGGTTGGACt                                                                                                                                      >  4:257664/4‑133 (MQ=255)

            gttcTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGT‑GTCTTACCGGGTTGGACTCAAGAc                                                                                                                                >  7:66964/4‑139 (MQ=255)

             ttcTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGT‑GTGTTAGCGGGGTGGGCGGAAGACg                                                                                                                               >  5:171752/3‑139 (MQ=255)

             ttcTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGT‑GTCTTACCGGGTTGGACTCAAGAc                                                                                                                                >  7:300820/3‑138 (MQ=255)

             ttcTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGT‑GTCTTACCGGGTTGGACTCAAGAc                                                                                                                                >  4:33593/3‑138 (MQ=255)

             ttcTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGT‑GTCTTACCGGGTTGGACTCAAGACg                                                                                                                               >  3:49793/3‑139 (MQ=255)

             ttcTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTAGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGT‑GTCTTACCCGGTTGGCCTCAAGAc                                                                                                                                >  1:31906/3‑138 (MQ=255)

             ttcTTCTAGTGTAGCCGTACTTAGGCCACCACTACAAGAACTCTGTAGCACCGAGTACATACCACGCTCAGCTAATACTGATACCAGTGGCTGCTGCCAGAGGAGATAAGACGT‑TTCTGACATGGTTGGACTCAacac                                                                                                                                >  4:59182/3‑138 (MQ=255)

               cTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGT‑GTCTTACCGGGTTGGACTCAAGACGa                                                                                                                              <  8:35890/138‑1 (MQ=255)

                  cTAGTGTAGCCGTAGTTAGGCCCCCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGT‑GTCTTACCGGGTTGGCCGCAAGCCGATAGt                                                                                                                          >  6:98639/1‑139 (MQ=255)

                  cTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGT‑GTCTTACCGGGTTGGACTCAAGACGATAg                                                                                                                           <  7:2575/138‑1 (MQ=255)

                     gtgtAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGT‑GTCTTACCGGGTTGGAGTCAAGACGATAGTTa                                                                                                                        <  5:28822/138‑1 (MQ=255)

                     gtgtAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGT‑GTCTTACCGGGTTGGACTCAAGACGATAGTTa                                                                                                                        <  1:91719/138‑1 (MQ=255)

                     gtgtAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGT‑GTCTTACCGGGTTGGACTCAAGACGATAGTTa                                                                                                                        <  4:115737/138‑1 (MQ=255)

                     gtgtAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGT‑GTCTTACCGGGTTGGACTCAAGACGATAGTTAc                                                                                                                       >  5:50877/1‑139 (MQ=255)

                       gtAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGT‑GTCATACCGGGTTGGACTCAAGACGATAGTTACCg                                                                                                                     >  3:251395/1‑139 (MQ=255)

                          gCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGT‑GTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGa                                                                                                                   >  7:83770/1‑138 (MQ=255)

                          gCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGT‑GTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGAt                                                                                                                  <  1:124009/139‑1 (MQ=255)

                           ccGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGT‑GTCTTACCGGGTTGGACTCAAGACGATAGTTAc                                                                                                                       >  6:76709/1‑133 (MQ=255)

                           ccGTAGTTAGGCCAACACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGT‑GTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATa                                                                                                                 <  8:56105/139‑1 (MQ=255)

                                     gCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGT‑GTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGa                                                                                                                   >  8:104288/1‑127 (MQ=255)

                                          cacTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGT‑GGCTTAACGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCgg                                                                                                  >  3:210345/1‑139 (MQ=255)

                                            cTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGT‑GTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGc                                                                                                <  3:88846/139‑1 (MQ=255)

                                              tCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGT‑GTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCt                                                                                               >  7:17743/1‑138 (MQ=255)

                                                       ctGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGT‑GTCTTACCGGGTTGGACTCAAGACGATAGTTa                                                                                                                        <  8:230057/104‑1 (MQ=255)

                                                       ctGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGT‑GTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACgggggg                                                                                     >  7:196848/1‑139 (MQ=255)

                                                       ctGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGT‑GTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACgggggg                                                                                     >  3:69835/1‑139 (MQ=255)

                                                       ctGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGT‑GTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACgggggg                                                                                     >  6:113925/1‑139 (MQ=255)

                                                        tGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGT‑GTCTTACCGGGTTGGGCTCAAGACGATAGTTTACGGAGAAGGCACAGCGGTCGGGCTGGACGGGGGGt                                                                                    >  1:151319/1‑139 (MQ=255)

                                                        tGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGT‑GTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGgcac                                                                                                           >  6:143409/1‑114 (MQ=255)

                                                                 gCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGT‑GTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGcaca                                                                           <  1:241095/139‑1 (MQ=255)

                                                                  ccTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGT‑GTGTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGACCGGGGGGTTCGTGcccac                                                                          >  5:72889/1‑139 (MQ=255)

                                                                  ccTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGT‑GTCTTTCCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCAAAGCCGTCGGGCTGAACGGGGGGTTCGTGcacaa                                                                          >  2:190978/1‑138 (MQ=255)

                                                                  ccTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGT‑GTCTTACCGGGTTGGACt                                                                                                                                      >  6:260408/1‑79 (MQ=255)

                                                                  ccTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGT‑GTCTTACCGGGGTGGACTCAAGACGATAGTTAACGGATAAGGCACAGCGGTAGGGCTGAACGGGGGGTTCGTGgacac                                                                          >  6:307155/1‑139 (MQ=255)

                                                                  ccTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGT‑GTCTTACCGGGGTGGACTCAAGACGATAGGTACCGGCTAAGGCACCGCGGGCGGGCTGAACGGGGGGTTGGTGgacac                                                                          >  8:267696/1‑139 (MQ=255)

                                                                    tACATACCTCGCTCTGCTAAACCTGTTACCACTGGCTGCTGCCAGTGGCGAAAAGTCGT‑GGGTTAACCGGTTGTACTCAATAACATAGTTAACCGATCAGGCACAGCCGTCGGGCTGAAACGCGGCTTCCTTcacacac                                                                        >  4:278411/1‑138 (MQ=255)

                                                                      cATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGT‑GTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGcc                                                                      <  2:61302/139‑1 (MQ=255)

                                                                      cATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGT‑GTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGcc                                                                      <  4:18140/139‑1 (MQ=255)

                                                                      cATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGT‑GTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGcc                                                                      <  3:182045/139‑1 (MQ=255)

                                                                       aTACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGT‑GTCTTACCGGGTTGGCCTCAAGACGAATGTTAACGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGcc                                                                      >  7:11777/1‑138 (MQ=255)

                                                                           cTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGT‑GTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCt                                                                 >  7:41692/1‑139 (MQ=255)

                                                                             cGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGGTGTTCCCGGGTGGGACTCACGCCGTGAGTTACCGGATAAGGCCCGGCGGGCGGGCGGAGCGGGGGGTGCGGGCACCCAGCCCGGCtt                                                                >  2:41489/1‑139 (MQ=255)

                                                                             cGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGT‑GTCTTTCCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACCGCCCAGCATg                                                               >  1:289095/1‑139 (MQ=255)

                                                                             cGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGT‑GTCTTACCGGGTTGGACTCAAGACGATAGTTa                                                                                                                        >  7:235251/1‑82 (MQ=255)

                                                                             cGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGT‑GTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAgg                                                                                                              <  1:100351/92‑1 (MQ=255)

                                                                             cGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGT‑GTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTg                                                               >  3:27224/1‑139 (MQ=255)

                                                                             cGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGT‑GTCTTACCGGGGTGGCCTCAAGACGATAGTTACCGGATAAGGCACCGCGGTCGGGCGGAACGGGGGGTTCGTGCCCCCAGCCCAGCGTg                                                               >  8:248199/1‑139 (MQ=255)

                                                                              gCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGT‑GTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTgg                                                              >  2:8598/1‑139 (MQ=255)

                                                                               ctctGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGT‑GTCTTACCGGGTTGGACTAAAGACGATAGTTTCCGGATAAGGCCCAGCGGTCGGGCGGAACGGGGGGTTCGTGCACACCGCCCAGCTTg                                                               >  6:190426/1‑137 (MQ=255)

                                                                                    ctcaTCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGT‑GTCTTCCCGGGGTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAAC‑GGGGGTTCGTGCACACAGCCCAGCTTGGAGCGaa                                                        <  6:21548/135‑1 (MQ=255)

                                                                                    cTAATGCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGT‑GTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGa                                                         <  5:15018/138‑1 (MQ=255)

                                                                                    cTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGT‑GTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGaa                                                        <  4:297629/139‑1 (MQ=255)

                                                                                    cTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGT‑GTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGaa                                                        <  8:128041/139‑1 (MQ=255)

                                                                                    cTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGT‑GTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGaa                                                        <  1:173219/139‑1 (MQ=255)

                                                                                    cTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGT‑GTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGaa                                                        <  4:21071/139‑1 (MQ=255)

                                                                                    cTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGT‑GTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGaa                                                        <  7:214363/139‑1 (MQ=255)

                                                                                    cTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGT‑GTCTTACCGGGGTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGa                                                         <  2:29341/138‑1 (MQ=255)

                                                                                    cTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGG‑GTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGaa                                                        <  8:293306/139‑1 (MQ=255)

                                                                                    cTAATCCTCTTACCAGTGGCTGCTGCCAGTTGCGATAAGTCGT‑GGCGTACCGGCTGGGACTCAAGACGATAGTTACCGGATATGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGa                                                         <  4:236226/138‑1 (MQ=255)

                                                                                       aTCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGT‑GTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAAc                                                       <  5:88734/137‑1 (MQ=255)

                                                                                       aTCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGT‑GTCTTAACGGGTTGGACTCAAGACGATAGTTTCCGGATAAGGGACAGCGGTCGGGCTGAACGGGGGGTTCGTGCCCCAAGCCCAGCGTGGAGCGAACGa                                                     >  6:168218/1‑139 (MQ=255)

                                                                                          cTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGT‑GTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCt                                                  <  4:133959/139‑1 (MQ=255)

                                                                                            gTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGT‑GTCTTACCGGGTTGGACTCAATACGATAGTTACCGGATAAGGCACAGCGGTCGCGCTGAACGGGGGGTTCGTTCACCCAGCCCAGCTTGGAGCGAACGCCCGac                                                >  4:78302/1‑139 (MQ=255)

                                                                                            gTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGT‑GTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACgggggg                                                                                     <  2:21705/102‑1 (MQ=255)

                                                                                            gTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGT‑GTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACgggg                                                                                       >  6:279773/1‑100 (MQ=255)

                                                                                            gTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGT‑GTCTTACCGGGGTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGATCGAACGACCTa                                                 >  7:156262/1‑138 (MQ=255)

                                                                                             ttACCAGTGGCTGCTGCCAGTGGCGATAAGTCGT‑GTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTaca                                               <  1:218415/139‑1 (MQ=255)

                                                                                              tACCAGTGGCTGCTGCCAGTGGCGATAAGTCGT‑GTCTTACGGGGGTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTaca                                               <  7:279618/138‑1 (MQ=255)

                                                                                              tACCAGTGGCTGCTGCCAGTGGCGATAAGTCGT‑GTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTaca                                               <  1:69183/138‑1 (MQ=255)

                                                                                                  aGTGGCTGCTGCCAGTGGCGATAAGTCGT‑GTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGaa                                          <  8:80316/139‑1 (MQ=255)

                                                                                                  aGTGGCTGCTGCCAGTGGCGATAAGTCGT‑GTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGaa                                          <  1:192926/139‑1 (MQ=255)

                                                                                                  aGTGGCTGCTGCCAGTGGCGATAAGTCGT‑GTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGaa                                          <  3:230938/139‑1 (MQ=255)

                                                                                                    tGGCTGCTGCCAGTGGCGATAAGTCGT‑GTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGAc                                                    <  1:49113/127‑1 (MQ=255)

                                                                                                    tGGCTGCTGCCAGTGGCGATAAGTCGT‑GTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACt                                        <  4:151575/139‑1 (MQ=255)

                                                                                                     ggCTGCTGCCAGTGGCGATAAGTCGT‑GTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGCCCAACACCGAACt                                        >  8:173346/1‑138 (MQ=255)

                                                                                                      gctgctGCCAGTGGCGATTAGTCGT‑GTCTTTGCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGACCAGCTTGGAGCGAACGACCTACACCGAACTga                                      <  6:236787/139‑1 (MQ=255)

                                                                                                      gctgctGCCAGTGGCGATAAGTCGT‑GTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTga                                      <  5:267232/139‑1 (MQ=255)

                                                                                                      gctgctGCCAGTGGCGATAAGTCGT‑GTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTga                                      <  7:141038/139‑1 (MQ=255)

                                                                                                        tggtgcCAGTGGCGATAAGTCGT‑GTATTACCGGGGTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTgaga                                    <  3:111788/136‑1 (MQ=255)

                                                                                                        tgctgcCAGGGGCGATCAGTCGT‑GTCTTACCGGGGTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCGCAGCTTGGAGCGAACGACCTACACCGAACTgaga                                    <  2:147065/139‑1 (MQ=255)

                                                                                                          ctgcCAGTGGCGATAAGTCGT‑GTCTTACCGGGTTGGACTCAAGACGATAGTTTCCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTGGGCGCGCACGACCTACCCCGAACTGCGAtg                                  >  5:200274/1‑138 (MQ=255)

                                                                                                          ctgcCAGTGGCGATAAGTCGT‑GTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGcaca                                                                           >  5:246370/1‑98 (MQ=255)

                                                                                                          ctgcCAGTGGCGATAAGTCGT‑GTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTaca                                               <  3:291293/126‑1 (MQ=255)

                                                                                                          ctgcCAGTGGCGATAAGTCGT‑GTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTgaga                                    <  5:221335/137‑1 (MQ=255)

                                                                                                          ctgcCAGTGGCGATAAGTCGT‑GTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTgaga                                    <  2:174268/137‑1 (MQ=255)

                                                                                                          ctgcCAGTGGCGATAAGTCGT‑GTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTgaga                                    <  3:153879/137‑1 (MQ=255)

                                                                                                          ctgcCAGGGGCGATAAGGCTT‑GTCTTACCGGGATGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGATCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTgaga                                    <  5:104103/137‑1 (MQ=255)

                                                                                                             ccAGTGGCGATAAGGCCG‑GTCTTACCGGGGTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTGCGTTCACACAGCCCAGCTTGGAGCGAACGACCTAAACCGAACTGAGATACCt                               <  2:113845/139‑1 (MQ=255)

                                                                                                              cAGTGGCGATAAGTCGT‑GTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGTTAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTa                              <  2:26906/139‑1 (MQ=255)

                                                                                                                     gATAAGTCGT‑GTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTg                       <  3:133148/139‑1 (MQ=255)

                                                                                                                     gATAAGTCGT‑GTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTg                       <  5:127725/139‑1 (MQ=255)

                                                                                                                     gATAAGGCGT‑GTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTg                       <  8:151678/139‑1 (MQ=255)

                                                                                                                      aTAAGTTGT‑GTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGt                        >  1:261297/1‑137 (MQ=255)

                                                                                                                       tAAGTCGT‑GTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTgaga                                    <  3:310505/124‑1 (MQ=255)

                                                                                                                       tAAGTCGT‑GTCCTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAg                     <  2:241951/139‑1 (MQ=255)

                                                                                                                              t‑gtCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACgggggg                                                                                     <  8:192226/68‑1 (MQ=255)

                                                                                                                                      ccGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAgcgc       >  8:207257/1‑139 (MQ=255)

ATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGT‑GTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGC  >  pSIJ663_pHT315_araE_xy/5035‑5311

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`