BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	pSIJ663_pHT315_araE_xy	6,673	A→C	6.9%	intergenic (+1942/–)	ampR → / –	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	pSIJ663_pHT315_araE_xy	6,673	0	A	C	6.9%	5955.0 / 49.7	2150	intergenic (+1942/–)	ampR/–	–/–
Reads supporting (aligned to +/- strand): ref base A (1097/803); new base C (7/192); total (1106/1044)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 2173 total aligned reads displayed.

GGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAG  >  pSIJ663_pHT315_araE_xy/6536‑6811

gggCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAg                                                                                                                                           >  6:68244/1‑139 (MQ=255)

gggCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAg                                                                                                                                           <  5:149377/139‑1 (MQ=255)

   ccttGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTg                                                                                                                                        <  8:24495/136‑1 (MQ=255)

   ccATGTTGATGAGTCAACCCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTg                                                                                                                                        <  5:86000/139‑1 (MQ=255)

   ccATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTg                                                                                                                                        <  3:159107/139‑1 (MQ=255)

   ccATGTTGAGGCGTCAAACCAAACAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGCACGCGCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATAAAAGcgg                                                                                                                                        <  2:203618/139‑3 (MQ=255)

   ccATGTGGAGGCCTCAAACCAAAGCAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTg                                                                                                                                        <  2:69719/139‑1 (MQ=255)

      tGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTgggc                                                                                                                                     >  4:77063/1‑138 (MQ=255)

      tGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGa                                                                                                                                     >  4:311915/1‑139 (MQ=255)

        ttGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGGGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACa                                                                                                                                   <  3:35219/139‑1 (MQ=255)

          gatgaGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTTGCTCGGCACTATCATCAGCCTGTTGAGCTGCAGCGAATAAAGCTGGTACAtt                                                                                                                                 >  1:100651/1‑139 (MQ=255)

           atgaGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTg                                                                                                                                >  2:180394/1‑139 (MQ=255)

                tCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACa                                                                                                                                   <  6:197242/131‑1 (MQ=255)

                tCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAg                                                                                                                           >  3:121273/1‑139 (MQ=255)

                tCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAg                                                                                                                           >  4:270406/1‑139 (MQ=255)

                  aaaCCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCCCTTTCATCAGCCTGTTGAGCTGCCGCGATTAAAGCTGGGACATTGCTCAggg                                                                                                                         >  1:285531/1‑138 (MQ=255)

                  aaaCCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAgg                                                                                                                          <  3:176791/138‑1 (MQ=255)

                  aaaCCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAgg                                                                                                                          <  7:184030/138‑1 (MQ=255)

                        aaGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCCGCGATTAAAGCTGGGACATTGCTCAGGCCGGtt                                                                                                                    >  5:199241/1‑138 (MQ=255)

                         aGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTaa                                                                                                                  >  1:74816/1‑139 (MQ=255)

                         aGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTaa                                                                                                                  >  1:248077/1‑139 (MQ=255)

                         aGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTaa                                                                                                                  >  6:86886/1‑139 (MQ=255)

                         aGAAAACCCCCTTCGCCGGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGCCATTGCTCAGGCCGGTTaa                                                                                                                  >  8:143216/1‑139 (MQ=255)

                              aCCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTcg                                                                                                             <  4:223025/139‑1 (MQ=255)

                              aCCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTcg                                                                                                             <  8:80370/139‑1 (MQ=255)

                              aCCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTcg                                                                                                             <  2:5406/139‑1 (MQ=255)

                              aCCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGCCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTcg                                                                                                             <  7:252735/139‑1 (MQ=255)

                                  ccTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGc                                                                                                         <  3:80576/139‑1 (MQ=255)

                                   cTTCGCCTGGGTATTATTGTGTGGCGTACGCTCGCCGGAAACATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGcc                                                                                                        <  3:198625/139‑1 (MQ=255)

                                   cTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGTGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGcc                                                                                                        <  3:243755/139‑1 (MQ=255)

                                   cTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCTATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGcc                                                                                                        <  5:279140/139‑1 (MQ=255)

                                   cTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGcc                                                                                                        <  7:60037/139‑1 (MQ=255)

                                   cTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGcc                                                                                                        <  8:224553/139‑1 (MQ=255)

                                   cTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGcc                                                                                                        <  2:118793/139‑1 (MQ=255)

                                   cTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCAATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGcc                                                                                                        <  6:302327/139‑1 (MQ=255)

                                   cTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTGGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGcc                                                                                                        <  8:177771/139‑1 (MQ=255)

                                            gggATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGa                                                                                               >  4:50871/1‑139 (MQ=255)

                                            gggATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGa                                                                                               >  3:61087/1‑139 (MQ=255)

                                            gggATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGa                                                                                               >  6:257143/1‑139 (MQ=255)

                                            gggATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGAGTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGa                                                                                               >  1:186081/1‑139 (MQ=255)

                                              gtttattGTGGGGCGTCCGCGCGCCGTAAATAAACGGCATAAACCAAAGTGGCTCGGAAGTTTCATCAGCCTGTTGAGCTGCAGCTATTAACGCTGGGACATTGCTCCGGCGGGTTAATTTCGCGGCCCAATCCAGaca                                                                                             <  4:158410/137‑1 (MQ=255)

                                                 tattGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGTGATTAAAGCTg                                                                                                                                        <  5:235132/93‑1 (MQ=255)

                                                 tattGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACacga                                                                                          >  2:276608/1‑139 (MQ=255)

                                                 tattGTGGGGCGAACGCCCGCCGGAAAGATAAGCCACAAACCAAACTGGCGCGCCACTTTAATCAGCCTGTTGCGCTGCAGCGTTTAACGCGGGGAAATTGCTCAGGCCGGTTAATTTCGCGGCCAAATCCAGACacg                                                                                           <  6:227260/138‑1 (MQ=255)

                                                   gagccgggCGTCCGCTCGCCGTAAAGATACGCCAAAAACCATAGGGGCGGGGCAGTTTCCTCAGCTTTTTGCGGGGCTGCGTTTAATGGGGGGACATTGCTCGGGCCGGTTAATTTCGCGCCCCAATCCAGACacgacg                                                                                        <  8:262773/133‑1 (MQ=255)

                                                     gtgtgGCGCACGCCCGCGGTAAACATAAGCCACAAACAAAACTGCCGCGGCACTTTCACCAGCCGGTTGCGCGGCAGCTATAAAAGCGGGTCCATGCCCCTGGCCGGTAAATTCCGCGGCCCAATCCAGACACGACGCt                                                                                      <  2:226640/139‑1 (MQ=255)

                                                      tgtgGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAgg                                                                                                                          >  2:90740/1‑102 (MQ=255)

                                                      tgtgGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGCCACGACGCTg                                                                                     >  1:143357/1‑139 (MQ=255)

                                                      tgtgGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACGCTg                                                                                     >  4:195840/1‑139 (MQ=255)

                                                        tgGCGTACGCTCGCCGGAAATATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCCGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACGATgaa                                                                                   >  6:45805/1‑137 (MQ=255)

                                                        tgGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGAc                                                                                                                                    >  5:47178/1‑90 (MQ=255)

                                                        tgGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACa                                                                                                                                   >  7:164740/1‑91 (MQ=255)

                                                        tgGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAgg                                                                                                                          <  8:117680/100‑1 (MQ=255)

                                                        tgGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTcg                                                                                                             >  5:258653/1‑113 (MQ=255)

                                                        tgGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTcg                                                                                                             <  1:229807/113‑1 (MQ=255)

                                                        tgGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGcc                                                                                                        <  6:147479/118‑1 (MQ=255)

                                                        tgGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACGCTGCa                                                                                   >  1:304688/1‑139 (MQ=255)

                                                        tgGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACGCTGCa                                                                                   >  3:220423/1‑139 (MQ=255)

                                                        tgGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACGCTGCa                                                                                   >  5:126334/1‑139 (MQ=255)

                                                        tgGCGTACGCTCGCCCGAAAGATAAGGCAGAAACCAAACAGGCTCGGCACTATCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAAACCAGAAACGACGCTGc                                                                                    >  7:204326/1‑138 (MQ=255)

                                                          gCGTCCGCGCGCCGTAAATAAAACCCACAAACCAAACTGGCTCGGCATAATCAACAGCCTGTTGCGCTCCAGCTATTAAAGCTGGCACTTTCCACAGGCGGGTTAATTTCGCGGCCAAATCCAGACACGACGCTGCACt                                                                                 <  8:65007/139‑1 (MQ=255)

                                                             tACGCTCGCCGGAAATATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAg                                                                              >  5:104473/1‑139 (MQ=255)

                                                             tACGCTCGCCGGAAAGATAAGGCAGAACCCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAAGCCAGACACGACGCTGCACTCAg                                                                              >  7:141466/1‑139 (MQ=255)

                                                             tACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAgg                                                                                                                          <  7:7688/95‑1 (MQ=255)

                                                             tACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTcg                                                                                                             <  8:201970/108‑1 (MQ=255)

                                                             tACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACCCGACGCTGCCCTCAg                                                                              >  3:306417/1‑139 (MQ=255)

                                                             tACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAg                                                                              >  7:271249/1‑139 (MQ=255)

                                                             tACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAg                                                                              >  7:113385/1‑139 (MQ=255)

                                                                    gCCGGAAAGAACAGGCACAAACCAACCTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAACGCTGGGACATTGCTCAGGCCGGTAAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATca                                                                         <  3:137501/137‑1 (MQ=255)

                                                                    gCCGCAAAGATAAGGCACAAACCAAACTGGCTCGGCACTTTCATCCGCCTGTTGAGCGGCAGCGATTAAAGCGGGGACATTGCTCAGGCGGGTTAATTGCGCGGCCAAATCCAGACACGACGCTGCACTCAGCATcaca                                                                       <  1:51210/139‑1 (MQ=255)

                                                                     ccGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACa                                                                                                                                   >  5:219240/1‑78 (MQ=255)

                                                                     ccGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATca                                                                         >  7:93596/1‑136 (MQ=255)

                                                                     ccGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAg                                                                      >  6:279174/1‑139 (MQ=255)

                                                                                      aaaCCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGt                                                     >  1:268306/1‑139 (MQ=255)

                                                                                      aaaCCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGt                                                     >  3:9895/1‑139 (MQ=255)

                                                                                      aaaCAAAACGGCCTCGGCACTTTAATCAGCCTGTTGTGCGGCAGCTATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGCCCAAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGt                                                     <  6:119446/139‑1 (MQ=255)

                                                                                            aaCTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCgg                                                >  8:291314/1‑138 (MQ=255)

                                                                                                 gCTCGCCACTTTCATCAGCCTGTTGTGCTGCTGCTATTAATGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCAAATCCAGACACGACGCTGCACTCAGCATCACAAACATTAAATGCCAACGTTGCGGTAgcgc                                          <  4:291250/139‑1 (MQ=255)

                                                                                                        aCTTTCATCAGCCGGTTGCGCTCCAGCGTTTAATGCTGGTACATTGCTCCGCCGGTTTAATTCCGCGGCCCAAGCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCaa                                   <  5:304500/139‑1 (MQ=255)

                                                                                                           ttCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACCCGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAg                                >  7:297224/1‑139 (MQ=255)

                                                                                                            tcatcaGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGc                               >  7:37489/1‑139 (MQ=255)

                                                                                                             cacccgCCTGGTGCGCTGCCCCTAATAAAGCGGGCACATTGCGCCGCCCGGTTAATTGCGCGCCCAAACCCACACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCt                              <  5:172567/134‑1 (MQ=255)

                                                                                                                 cgccgtttGAGCTGCTGCTATTAACGCTGGGACATTGCGCAGCCCGTTTAATTCCGCGGCCCAATCCCGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGt                          <  1:97465/133‑1 (MQ=255)

                                                                                                                 aGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCCGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATg                           >  1:166726/1‑138 (MQ=255)

                                                                                                                   ccTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTAc                        >  4:245393/1‑139 (MQ=255)

                                                                                                                     ttttGCGCTGCAGCGATTAACGCTGGGACTTTGCTCTGGCCGTTATATTGCGCGGCCAAAACCACACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACgg                      <  5:66634/137‑1 (MQ=255)

                                                                                                                      gTTGCGCTGCAGCTATAAAAGCGGGGACTTTGCTCAGGCCGGATAATTTCGCGCCCCAATCCCGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACg                       <  2:248959/137‑1 (MQ=255)

                                                                                                                      gTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGcc                                                                                                        <  3:257560/56‑1 (MQ=255)

                                                                                                                            cTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTcg                                                                                                             <  1:119832/45‑1 (MQ=255)

                                                                                                                             tGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCt              >  8:40984/1‑139 (MQ=255)

                                                                                                                               cAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTgg            >  2:160232/1‑139 (MQ=255)

                                                                                                                                 gggATTAAAGCTGGGACATTGCTCCGGGCGGTTAATTCCGCGGCACAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGgct          <  6:165239/137‑1 (MQ=255)

                                                                                                                                 gctaTTAATGCGGGGACATTGCACAGGCCGTTTAATTCCGCGGCCCTATCCCGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGgct          <  3:273478/136‑1 (MQ=255)

                                                                                                                                 gCGTTTAATGGTGGGACATTGCCCCGCCCGGTTAATTGCGCGGCCCAATCCCGCCACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGgct          <  4:118551/139‑1 (MQ=255)

                                                                                                                                 gCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGgct          <  1:21270/139‑1 (MQ=255)

                                                                                                                                 gCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGgct          <  8:161603/139‑1 (MQ=255)

                                                                                                                                 gCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGgct          >  3:288790/1‑139 (MQ=255)

                                                                                                                                 gCGATAAAAGCTGGGACATTGCTCAGGCGGGTTAATTCCGCGGCCCAATCCACACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGgct          <  4:134431/139‑1 (MQ=255)

                                                                                                                                    aTTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATcaca                                                                       >  7:232451/1‑75 (MQ=255)

                                                                                                                                     taaaaGCTGGCACATGCCGCCGGCCGGTTAATGACGCGCCCCAAACCACCCACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGgcttg        <  2:38030/135‑1 (MQ=255)

GGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAG  >  pSIJ663_pHT315_araE_xy/6536‑6811

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`