BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	pSIJ663_pHT315_araE_xy	6,689	A→C	9.7%	intergenic (+1958/–)	ampR → / –	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	pSIJ663_pHT315_araE_xy	6,689	0	A	C	9.7%	4650.2 / 77.0	1997	intergenic (+1958/–)	ampR/–	–/–
Reads supporting (aligned to +/- strand): ref base A (1089/602); new base C (17/217); total (1106/891)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 2021 total aligned reads displayed.

GTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCTGACAG  >  pSIJ663_pHT315_araE_xy/6551‑6827

 tCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAg                                                                                                                                           >  6:269718/1‑139 (MQ=255)

   aaaCCAAAGTAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAgg                                                                                                                                          <  5:269022/138‑1 (MQ=255)

   aaaCCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAgg                                                                                                                                          <  3:141798/138‑1 (MQ=255)

   aaaCCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAgg                                                                                                                                          <  8:246260/138‑1 (MQ=255)

   aaaCCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGc                                                                                                                                         >  1:2321/1‑139 (MQ=255)

   aaaCCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGACTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGc                                                                                                                                         >  1:250202/1‑139 (MQ=255)

                cccccTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTcgc                                                                                                                            <  3:160071/139‑1 (MQ=255)

                    cTTCGCGCGGGGATTATTGTGTGGCGTCCGCGCGCCGCAAAGAAACCGCACACACCAAACTGGCTCGGCACTTTAATCACCCTGTTGAGCGGCCGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGcc                                                                                                                        <  2:277813/139‑1 (MQ=255)

                    cTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGCCATTGCTCAGGCCGGTTAATTTCGCGGcc                                                                                                                        >  7:236208/1‑139 (MQ=255)

                      tCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGccc                                                                                                                       >  1:35937/1‑138 (MQ=255)

                      tCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGccc                                                                                                                       >  1:80027/1‑138 (MQ=255)

                        ggcTGGGGATTTTTGGGTGCAGTCCGCTCGCCGAAAAGAAACGCCCCAAACCAACCTGGGGCGGCACTTTCATCAGCCTGTTGCGCTGCAGATATTACAGCTGGGACATTCCTCCGGCCGGTTAATTTCGCGGCCCAAt                                                                                                                    <  8:114803/137‑1 (MQ=255)

                        gCCTGGGTATTATTGTGGGGCGTACGCGCGCCGGAAAGATAAGGCAGAAACCAAACTGGCGCGGCACTTTCATCCGCCTGTTGAGCTGCAGCGATTAACGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAAt                                                                                                                    <  4:106869/139‑1 (MQ=255)

                        gCCTGGGGATTATTGTGTGGCGTACGCGCGCCGGAAAGATAAGGCAGAAACCAAACTGGCGCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAAt                                                                                                                    <  2:91531/139‑1 (MQ=255)

                          cTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCCCTTTCATCAGCCTGTTGAGCTGCAGCGCTTAAAGCTGGGAGATTGCTCAGGCCGGTTAATTTCGCGGCCCAATcc                                                                                                                  >  8:38732/1‑139 (MQ=255)

                               gATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAACGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGaca                                                                                                             <  7:29138/139‑1 (MQ=255)

                                     tgtgggGCGTACGCTCGCCGTAACTAAACGGCACAAACCAAACTGGCTCGGAACTTGCATCAGCCTGTTGAGCTCCAGCGATTAACGCTGGGACTTTGCCCCGGCCGGTTAATTGCGCGGCCCAATCCAGACacgacg                                                                                                        <  4:264199/138‑1 (MQ=255)

                                      gtgggGCGTACGCGCGCCGTAAAGATACGGCAGAAACCAAAGGGGCGCGGAACTTTCATCAGCGGTTGGCGCTGCTGCGATTAACGCGGGGACATTGCTCCGGCGGGTTAATTTCGCGGCCCAATCCAGACACGACGCt                                                                                                      <  8:296246/139‑1 (MQ=255)

                                       tgtgGCGTATGCTCGCCGGAAAGATAAGGCAGAACCCAAACTGGCTCGGCACTGTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCACTCCAGACGCGACGCTg                                                                                                     >  5:287616/1‑139 (MQ=255)

                                       tgtgGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCCCTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACGCTg                                                                                                     >  1:213382/1‑139 (MQ=255)

                                       tgtgGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACGCTg                                                                                                     >  1:10152/1‑139 (MQ=255)

                                       tgtgGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACGCTg                                                                                                     >  2:51797/1‑139 (MQ=255)

                                       tgtgGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATAAAAGCTGGGACATTGCTCAGGCCGGTTACTTTCGCGGCCCAATCCAGACACGACGCTg                                                                                                     >  1:274297/1‑139 (MQ=255)

                                         tgGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTcg                                                                                                                             >  2:229807/1‑113 (MQ=255)

                                         tgGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACGCTGcg                                                                                                   >  5:76874/1‑138 (MQ=255)

                                         tgGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACGCTGCa                                                                                                   >  6:238761/1‑139 (MQ=255)

                                         tgGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACGCTGCa                                                                                                   >  7:187565/1‑139 (MQ=255)

                                         tgGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACGCTGCa                                                                                                   >  4:132317/1‑139 (MQ=255)

                                         tgGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACGCTGCa                                                                                                   >  1:290828/1‑139 (MQ=255)

                                         tgGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATAAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCGGGTTAATTTCGCGGCCCAAt                                                                                                                    <  6:137327/122‑1 (MQ=255)

                                          ggCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACGCTGCAc                                                                                                  >  4:237607/1‑139 (MQ=255)

                                           gCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACGCTGCACt                                                                                                 <  7:209235/139‑1 (MQ=255)

                                           gCGTACGCTCGCCGGAAAGAAACGGCAGAAACCAACCTGGCGCGGCACTTTCCTCAGCCTGTTGAGCTGCAGCTATAAAAGCTGGGACATTGCTCCGGCCGGTTAATTTCGCGGCACAATCCAGACACGACGCTGCACt                                                                                                 <  3:249346/139‑1 (MQ=255)

                                           gCGTACGCGCGCCGGAAATAAACGGCAGAAACCAAACTGGCGCGGCACTTTCATCTGCCTGTTGAGCGGCCGCTATAAAAGCGGGGACATTGCTCCGGCGGGTTATTTTCGCGGCCCAATCCAGACACGACGCTGCACt                                                                                                 <  4:60648/139‑1 (MQ=255)

                                            cGTACGCTCGCCGTAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACGCTGCACt                                                                                                 >  3:260762/1‑138 (MQ=255)

                                            cGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACGCTGCACt                                                                                                 >  1:5595/1‑138 (MQ=255)

                                            cGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTc                                                                                                >  8:179080/1‑139 (MQ=255)

                                            cGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTc                                                                                                >  2:160919/1‑139 (MQ=255)

                                            cGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTc                                                                                                >  4:34448/1‑139 (MQ=255)

                                             gTACGCTCGCCGGAAAGATACGCCAGAAACCAACCGGGCGCGCCACTTTCATCCGCCTGTTGCGCTGCCGCTATAAACGCGGGGACATTGCGCCGGCCGGTTAATTCCGCGGCCCAATCCCCACACGACGCTGCACTCa                                                                                               <  8:58721/139‑1 (MQ=255)

                                              tACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTcg                                                                                                                             <  8:139528/108‑1 (MQ=255)

                                              tACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAg                                                                                              >  7:271249/1‑139 (MQ=255)

                                              tACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAg                                                                                              >  3:226875/1‑139 (MQ=255)

                                              tACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAg                                                                                              >  2:70853/1‑139 (MQ=255)

                                              tACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAg                                                                                              >  6:82562/1‑139 (MQ=255)

                                                 gCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGCCACGACGCTGCACTCAGCAt                                                                                           >  3:309479/1‑139 (MQ=255)

                                                   tCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCTGCCCAAACCAGACACGACGCTGCACTCAGCATca                                                                                         >  8:264463/1‑139 (MQ=255)

                                                   tCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATcc                                                                                         >  2:206376/1‑138 (MQ=255)

                                                     gCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATcaca                                                                                       <  3:123526/139‑1 (MQ=255)

                                                      ccGTAAATATACGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCGGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAg                                                                                      <  4:152117/139‑1 (MQ=255)

                                                                  ggaaGAAACCAACCGGGCGCGGCACTTTCATCAGCCTGTTGCGCTGCAGCGATAAAAGCGGGCACATTGCGCAGGCCGGTTAATTTCGCGGCCCAATCCAGCCACGACGCTGCACTCAGCATCACAGACATTAAATGcc                                                                          <  3:293644/136‑1 (MQ=255)

                                                                       aaaCCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGt                                                                     >  4:278433/1‑139 (MQ=255)

                                                                       aaaCCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGt                                                                     >  3:70107/1‑139 (MQ=255)

                                                                        aaCCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACACGCCGCTGCACTCAGCATCACAGACATTAAATGCCAACGtt                                                                    >  5:224229/1‑139 (MQ=255)

                                                                             aaCTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGcc                                                                                                                        >  5:51052/1‑82 (MQ=255)

                                                                              aCTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCgg                                                                >  4:294048/1‑137 (MQ=255)

                                                                                  gcgcgccACTTTCACCCGCCTGTTGAGCCGCCGCTATAAATGCTGGCACATTCCTCCGGCGGGTAAATTGCGCGGCCCAATCCCCACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAgcgc                                                          <  1:98210/133‑1 (MQ=255)

                                                                                  gCTCGGCACTTTCATCAGCCTGTGGAGCTGCAGCGATTAAAGCGGGGACATTGCTCAGGCCGGTAAATATCGCGGCCCAATCCACACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAgcgc                                                          <  7:105512/139‑1 (MQ=255)

                                                                                     cGGCACTTTCATCAGCCTGTTGAGCTGCAGAGATTAAAGCTGGGACATTGCTCAGGACGGTTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATAAAATGCCAACGTTGCGGTAGCGCATg                                                       >  7:166705/1‑139 (MQ=255)

                                                                                       gCACTTTCATCCGCCTGTGTAGCTGCAGCGATTAAAGCTGGTACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGc                                                     <  6:104473/139‑1 (MQ=255)

                                                                                         aCTTTCTACAGCCTGTGGCCCGCCAGCGATAAAAGCTGGGACATTGCTCCGGCCGGTAAATTTCGCGCCCAAATCCCCACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCaa                                                   <  5:109531/139‑1 (MQ=255)

                                                                                         aCTTTCATCTGCGTGTTGAGCGGCAGCGTTTAAAGGGGGGACATTGCGCAGGCGGGTTAATTGCGCGGCCAAATCCAGCCACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCaa                                                   <  7:35205/139‑1 (MQ=255)

                                                                                         aCTTTCATCAGCCTGTTGAGCTCCAGCTATTAAAGCGGGGACTTTGCTCTGGCGGGTTAATTTCGCGGCCAAATCCACACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCaa                                                   <  7:53874/139‑1 (MQ=255)

                                                                                              catccGCGTGTTTAGCTGCCGCGATAAACGCTGGGACATTGCCCAGGCCGGTTATTTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCt                                              <  8:222330/139‑1 (MQ=255)

                                                                                                  aGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTcg                                                                                                                             <  1:187994/56‑1 (MQ=255)

                                                                                                  aGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCCGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATg                                           >  1:166726/1‑138 (MQ=255)

                                                                                                  aGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATg                                           >  3:178747/1‑138 (MQ=255)

                                                                                                       gTTGAGCTGCCGCTATTAACGCTGGGACATTGCTCTGGCCGGTTAATTTCGCGCCCCAATCCACCCACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGgc                                     <  6:309275/139‑1 (MQ=255)

                                                                                                       gTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCaa                                                   <  5:149457/125‑1 (MQ=255)

                                                                                                       gTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGATGCGGTAGCGCATGGCAAAAGCTATGTACGgc                                     >  6:286262/1‑139 (MQ=255)

                                                                                                                cAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTcg                                                                                                                             >  5:244381/1‑42 (MQ=255)

                                                                                                                cAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTgg                            >  7:254160/1‑139 (MQ=255)

                                                                                                                cAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTgg                            >  3:277806/1‑139 (MQ=255)

                                                                                                                 aGCTATTAACGCTGGGACATTGCTCCGGCCGGTAAATTGCGCGGCCCAAGCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGgc                           <  3:95591/139‑1 (MQ=255)

                                                                                                                 aGCTATTAAAGCGGGGACATTGCTCAGGCGGGTTAATTTCGCGGCCAAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGgc                           <  8:79490/139‑1 (MQ=255)

                                                                                                                  gCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGgct                          <  8:161603/139‑1 (MQ=255)

                                                                                                                  gCGATTAAAGCTGGGACATTGCTCAGGCCGGCTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGgct                          >  5:129224/1‑139 (MQ=255)

                                                                                                                  gCGATAAATGCGGGGACATTGCTCCGGCCGGTAAATTTCGCGGCCAAATCCACACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGgct                          <  6:62096/139‑1 (MQ=255)

                                                                                                                  cctaTTAATGCTGGTAAATTGCGCTCGCGGGTTAATTTCGCGCCCAAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGgct                          <  3:50871/136‑1 (MQ=255)

                                                                                                                   ctaTTAACGCGGGGACATTGCTCAGGCCGGTAAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGgct                          <  2:183613/136‑1 (MQ=255)

                                                                                                                     aTTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGgcttgc                       >  4:86946/1‑139 (MQ=255)

                                                                                                                      taaaaGCTGGCACATGCCGCCGGCCGGTTAATGACGCGCCCCAAACCACCCACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGgcttg                        <  2:38030/135‑1 (MQ=255)

                                                                                                                       tAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAAt                                                                                                                    >  6:177824/1‑44 (MQ=255)

                                                                                                                         aaGCGGGGAAATTGCTCTGGCGGGTAATTTGCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCACATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTaa                   <  4:218905/139‑1 (MQ=255)

                                                                                                                          aGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGgcttgctt                     >  7:89951/1‑136 (MQ=255)

                                                                                                                               ggACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCaa                                                   >  6:152525/1‑101 (MQ=255)

                                                                                                                               ggACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACTGCGCTTTCTGGCTTGCTTAAGAAccc             >  5:17409/1‑139 (MQ=255)

                                                                                                                               ggACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGgcttg                        >  1:12193/1‑128 (MQ=255)

                                                                                                                               ggACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGgcttg                        <  2:12193/128‑1 (MQ=255)

                                                                                                                               ggACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAAccc             >  5:202036/1‑139 (MQ=255)

                                                                                                                               ggACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAAccc             >  5:308961/1‑139 (MQ=255)

                                                                                                                               ggACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAAccc             >  4:181345/1‑139 (MQ=255)

                                                                                                                               ggACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAAccc             >  6:214582/1‑139 (MQ=255)

                                                                                                                               ggACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAAccc             >  1:141662/1‑139 (MQ=255)

                                                                                                                                     tGCTCAGGCCGGTTAATTTCGCGGCCCAAGCCCGACACGCCGCTGCACTCAGCATCACAGCCATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCGTGCTTCAGCACCCTTCGcg       >  2:250689/1‑138 (MQ=255)

                                                                                                                                      gCTCCGGCGGGTTTATTTCGCGGCCCACTCCAGACACTACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGGTTGCTTAAGAACCCTTCGCt       <  7:14853/138‑1 (MQ=255)

                                                                                                                                      gCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCt       <  8:200845/138‑1 (MQ=255)

                                                                                                                                      ccTCCGGCGGGTTAATTGCGCGGCCCAATCCACACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCTg      <  3:208860/138‑1 (MQ=255)

                                                                                                                                       cTCCGGCCGGTTAATTGCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCTGa     <  7:123331/139‑1 (MQ=255)

                                                                                                                                          aGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCTGACAg  >  7:73281/1‑139 (MQ=255)

GTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTTCGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCTGACAG  >  pSIJ663_pHT315_araE_xy/6551‑6827

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`