BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	pSIJ663_pHT315_araE_xy	8,621	T→G	5.5%	intergenic (+3890/–)	ampR → / –	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	pSIJ663_pHT315_araE_xy	8,621	0	T	G	5.5%	8617.3 / 102.2	3308	intergenic (+3890/–)	ampR/–	–/–
Reads supporting (aligned to +/- strand): ref base T (1478/1576); new base G (240/9); total (1722/1586)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 3318 total aligned reads displayed.

TCTTCCACACTGTTCGGGAACTGGTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCGC‑GGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGA  >  pSIJ663_pHT315_araE_xy/8483‑8759

   tCCACACTGTTCGGGAACTGGTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGt                                                                                                                                          >  2:88406/1‑139 (MQ=255)

   tCCACACTGTTCGGGAACTGGTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGt                                                                                                                                          >  8:126831/1‑139 (MQ=255)

       cacTGTTCGGGAACTGGTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGGGGTTAGc                                                                                                                                      >  5:34939/1‑139 (MQ=255)

        acTGTTCGGGAACTGGTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCt                                                                                                                                     >  7:101540/1‑139 (MQ=255)

          tGTTCGGGAACTGGTCGGTGTCCCAGCCCAGATGCGCATCGCCCCGGTTGGCGTCGCCAGAACCGAACAGGCCAAGCGCACTGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGGGGGTAGCtt                                                                                                                                    >  7:309596/1‑138 (MQ=38)

            ttCGGGAACTGGTCGGTGTCCCAGCCCAGTTGCGCATCGCCCCGGTTGGCGTCGACAGCCCCGAACAGGCCCCGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGGGGTTAGCttga                                                                                                                                  >  4:224795/1‑136 (MQ=38)

            ttCGGGAACTGGTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGCACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGGGGGGAGCGTCa                                                                                                                                  >  2:282117/1‑138 (MQ=38)

            ttCGGGAACTGGTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTGGCttag                                                                                                                                  >  1:119719/1‑136 (MQ=255)

            ttCGGGAACTGGTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCaa                                                                                                                                 >  5:268778/1‑139 (MQ=255)

            ttCGGGAACTGGTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCaa                                                                                                                                 >  6:200087/1‑139 (MQ=255)

            ttCGGGAACTGGTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCaa                                                                                                                                 >  5:242103/1‑139 (MQ=255)

            ttCGGGAACTGGTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCaa                                                                                                                                 >  7:94919/1‑139 (MQ=255)

            ttCGGGAACTGGTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCaa                                                                                                                                 >  7:289149/1‑139 (MQ=255)

            ttCGGGAACTGGTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCa                                                                                                                                  >  8:267980/1‑138 (MQ=255)

            ttCGGGAACTGGTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCGATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGGGGTTTGCTTCaa                                                                                                                                 >  1:63583/1‑139 (MQ=255)

              cGGGAACTGGTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGGGGTTAGCTTCAATg                                                                                                                               >  1:171860/1‑139 (MQ=255)

                ggAACTGGTCGGTGTCCCAGCCCAGTTGCGGATCGCGACGGTTGGCGTCGACAGTACCGTACAGGCCAAGCGCTATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGtt                                                                                                                             <  1:147436/139‑1 (MQ=39)

                    cTGGTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGt                                                                                                                         >  1:100103/1‑139 (MQ=255)

                      ggTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGt                                                                                                                                          <  5:199497/120‑1 (MQ=255)

                      ggTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTTGCTTCAATGTTCAGttt                                                                                                                       >  7:205818/1‑139 (MQ=255)

                      ggTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGttt                                                                                                                       >  8:209169/1‑139 (MQ=255)

                      ggTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGtt                                                                                                                        >  7:148884/1‑138 (MQ=255)

                      ggTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGtt                                                                                                                        >  6:131246/1‑138 (MQ=255)

                      ggTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGtt                                                                                                                        >  8:55282/1‑138 (MQ=255)

                      ggTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCGTCAATGTTCAGtt                                                                                                                        >  6:244981/1‑138 (MQ=255)

                      ggTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATCATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGtt                                                                                                                        >  8:38019/1‑138 (MQ=255)

                        tCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTaa                                                                                                                     <  3:228708/139‑1 (MQ=255)

                             gtCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATctct                                                                                                                <  2:210196/139‑1 (MQ=255)

                              tCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATctct                                                                                                                <  8:168472/138‑1 (MQ=255)

                               cccagcccagTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCttt                                                                                                              >  7:226962/1‑139 (MQ=255)

                                 cagcccagTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCTGTGGCTATTTCATTATGGACAGAGTGACCTGCCGGCGTCGCGTGGTTATATTCAAGGTTCAGTTTAATCTCgtttt                                                                                                            >  7:187231/1‑139 (MQ=255)

                                 cagcccagTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGt                                                                                                                                          >  4:17796/1‑109 (MQ=255)

                                 cagcccagTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTTGCGTCAATTGTGAGTTTAATCTCttttt                                                                                                            >  4:262532/1‑139 (MQ=255)

                                   gcccagTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTcc                                                                                                          <  4:97739/139‑1 (MQ=255)

                                    cccagTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCa                                                                                                         <  6:79947/139‑1 (MQ=255)

                                       agTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGAc                                                                                                      <  1:233629/139‑1 (MQ=255)

                                        gTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTTCCAGCGTCGCGTGGGGAGCTTCAATGTTGAGTTTAATCTCTTTTTGCAGGcc                                                                                                     >  1:42723/1‑139 (MQ=255)

                                        gTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTTGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGAcc                                                                                                     >  1:89553/1‑139 (MQ=255)

                                        gTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCa                                                                                                                                  <  3:56819/110‑1 (MQ=255)

                                        gTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGAc                                                                                                      >  2:41761/1‑138 (MQ=255)

                                        gTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTAAGTTTAATCTCTTTTTCCAGAcc                                                                                                     >  3:29882/1‑139 (MQ=255)

                                            cgcATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCaaa                                                                                                  <  3:157018/138‑1 (MQ=255)

                                            cgcATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCaaa                                                                                                  <  3:34110/138‑1 (MQ=255)

                                                   ccACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGGGGTTAGCTGCa                                                                                                                                  >  3:122688/1‑99 (MQ=255)

                                                     aCGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAgg                                                                                        <  6:107009/139‑1 (MQ=255)

                                                     aCGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAgg                                                                                        <  3:269738/139‑1 (MQ=255)

                                                     aCGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAgg                                                                                        <  4:155685/139‑1 (MQ=255)

                                                     aCGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAgg                                                                                        <  2:166550/139‑1 (MQ=255)

                                                     aCGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAgg                                                                                        <  5:126571/139‑1 (MQ=255)

                                                     aCGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAgg                                                                                        <  8:187638/139‑1 (MQ=255)

                                                     aCGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTG‑CAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGa                                                                                       <  7:124199/139‑1 (MQ=255)

                                                     aCGGTGGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGGCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAgg                                                                                        <  6:177692/139‑1 (MQ=255)

                                                         ttGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCa                                                                                                                                  >  1:211249/1‑93 (MQ=255)

                                                             cGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGGGGGTTGCTTGAATGTTCAGGTTAATATGTTTTTGCAGACCCAACAGGTTCAGGCAGccc                                                                                  >  4:203641/1‑136 (MQ=255)

                                                                 gACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTTGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGAc                                                                             <  7:16359/138‑1 (MQ=255)

                                                                   cataaCCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGt                                                                          <  5:168572/136‑1 (MQ=255)

                                                                       aCCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAgg                                                                                        <  7:69374/121‑1 (MQ=255)

                                                                       aCCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTcgc‑g                                                                     <  6:157289/139‑1 (MQ=255)

                                                                       aCCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTcgc‑g                                                                     <  8:152797/139‑1 (MQ=255)

                                                                           aaCAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGCCCACACTGTTTCAGGCAGCCATAGACCGTCGC‑GGCAt                                                                 >  2:10785/1‑139 (MQ=255)

                                                                           aaCAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCGC‑GGCAt                                                                 <  2:25031/139‑1 (MQ=255)

                                                                           aaCAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGGGGGTAGCTTCAATGTTCAGTTTTATCTCTTTTTCCAGACCCAACGGTTTCAGGAAGGCATAGACCGGCGC‑GGCAt                                                                 >  4:280507/1‑139 (MQ=255)

                                                                                gCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTTATCTCTTTTTCCAGCCCAAACGGTTTCAGGGAGCCCTAGACCCTCGC‑GGCATCGTaa                                                            >  3:105226/1‑139 (MQ=255)

                                                                                gCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCGC‑GGCATCGTaa                                                            <  4:243994/139‑1 (MQ=255)

                                                                                  cAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCGC‑GGCATCGTAATc                                                          <  6:219207/139‑1 (MQ=255)

                                                                                     gcgcAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTaa                                                                                                                     <  8:73182/78‑1 (MQ=255)

                                                                                     gcgcAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCGC‑GGCATCGTAATCata                                                       >  6:294323/1‑139 (MQ=255)

                                                                                     gcgcAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGGGGGTTGCTTGAATTTTAAGTTTTATCTCTTTTTTCAGACCCAACAGTTTAAGGTAGCCAGAGACCCTCGCGGGCATAGTAATCa                                                         >  2:63681/1‑138 (MQ=255)

                                                                                          aTGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCaa                                                                                                                                 <  5:222076/61‑1 (MQ=255)

                                                                                          aTGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCaaa                                                                                                  <  6:270528/92‑1 (MQ=255)

                                                                                          aTGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGa                                                                                       <  4:100164/103‑1 (MQ=255)

                                                                                          aTGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGCAGCCATAGACCGTCGC‑GGCATCGTAATCATATTGAt                                                  >  1:193996/1‑139 (MQ=255)

                                                                                          aTGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCGC‑GGCGTCGTAATCATATTGAt                                                  >  3:76700/1‑139 (MQ=255)

                                                                                          aTGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCGC‑GGCATCGTAATCATATTGAt                                                  >  5:145982/1‑139 (MQ=255)

                                                                                          aTGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATATCTTTTTCCAGACCaaa                                                                                                  >  8:287927/1‑92 (MQ=255)

                                                                                          aTGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGGGGTTAGGGTGAATGTTGAGTTTAATGTAATTTTGCAGACCAAACTGTTTCAGGAAGCCAAAGACCCTACC‑GGCCACGTAATCATATTTAt                                                  >  2:229382/1‑139 (MQ=255)

                                                                                                gTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTTGCTTCAATTTTGAGTTTAATCTGTTTTTCCACACCAAACAGTTTAAGGAAGCCATATACCGTCGC‑GGCATCGTAATCATAGTGATGTTTgg                                            >  6:60740/1‑139 (MQ=255)

                                                                                                gTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGGTTGAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCAAAGACCGTCGC‑GGCATCCTAATCATATTTAAGTTTgg                                            >  1:29524/1‑139 (MQ=255)

                                                                                                gTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCa                                                                                                                                  >  6:35782/1‑54 (MQ=255)

                                                                                                gTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCGC‑GGCATCGTAATCATATTGATGTTTgg                                            >  7:51662/1‑139 (MQ=255)

                                                                                                 tGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCGC‑GGCATCGTAATCATATTGATGTTTGGt                                           <  3:206512/139‑1 (MQ=255)

                                                                                                 tGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCGC‑GGCATCGTAATCATATTGATGTTTGGt                                           <  2:137753/139‑1 (MQ=255)

                                                                                                  ggCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCGC‑GGCATCGTAATCATATTGATGTTTGGTc                                          <  5:86137/139‑1 (MQ=255)

                                                                                                   gCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCGC‑GGCATCGTAATCATATTGATGTTTGGTc                                          <  3:284566/138‑1 (MQ=255)

                                                                                                   gCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTAAGGAAGCCATAGACCGTCGC‑GGCATCGGAATCATATTTATGTTTGGtgg                                         >  7:268078/1‑137 (MQ=255)

                                                                                                       tttCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCATCAATGTTCAGTTTAATAGCTTTTTACAGACCAAACTGTTTCAGGAAGCCATAGACCGTCGC‑GGCATCGTAATCAAATTTATTTTTGGTAGGTTc                                     >  7:244535/1‑139 (MQ=255)

                                                                                                           atgatgGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCGC‑GGCATCGTAATCATATTGATGTTTGGTCGGTTCTTg                                  <  5:288895/138‑1 (MQ=255)

                                                                                                             gatgGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGGTTGAATGTTCAGTTTAATCTCTTTTTGCAGACCAATCTGTTTCAGGAAGCCATAGACCGTCGC‑GGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCgg                               >  4:138554/1‑139 (MQ=255)

                                                                                                              atgGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGa                                                                                       >  4:117213/1‑83 (MQ=255)

                                                                                                                 gAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCGC‑GGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGttt                            <  8:226962/138‑1 (MQ=255)

                                                                                                                 gAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCGC‑GGCATAGTaa                                                            >  1:259609/1‑106 (MQ=255)

                                                                                                                  aaaGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAgg                                                                                        >  4:42385/1‑78 (MQ=255)

                                                                                                                  aaaGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCGC‑GGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCg                          <  4:30097/139‑1 (MQ=255)

                                                                                                                        tGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTGCAGACCAAACTGTTTCAGGAAGCCATATACCGTCGC‑GGCATCGTAATAATATTGTTTTTTTGTCGGTTTTTGCGCTGTCgcgtat                     >  8:242141/1‑133 (MQ=255)

                                                                                                                         gACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGCCCGTCGC‑GGCCTCGGAATCATATTGATGTTTGGTAGGTTCTTGCGGTTTCGGTTCGa                    >  3:136940/1‑138 (MQ=255)

                                                                                                                               ccAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCGC‑GGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCa              <  3:251239/138‑1 (MQ=255)

                                                                                                                                cAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCAAGACCAAACTGTTTCAGGAAGCCATAGACCGTCGC‑GGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTAGATAAGCAAc            >  2:19048/1‑139 (MQ=255)

                                                                                                                                cAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTATTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCGC‑GGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAAc            <  4:7150/139‑1 (MQ=255)

                                                                                                                                  gCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCGC‑GGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTGCGATAAGCAACGt          <  5:60740/139‑1 (MQ=255)

                                                                                                                                          tGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCGC‑GGCATCGTAATCATATTGATGTTTGGGCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGa  <  3:179850/139‑1 (MQ=255)

TCTTCCACACTGTTCGGGAACTGGTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCGC‑GGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGA  >  pSIJ663_pHT315_araE_xy/8483‑8759

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`