BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	pSIJ663_pHT315_araE_xy	8,839	T→A	5.1%	intergenic (+4108/–)	ampR → / –	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	pSIJ663_pHT315_araE_xy	8,839	0	T	A	5.1%	5041.3 / 43.7	1683	intergenic (+4108/–)	ampR/–	–/–
Reads supporting (aligned to +/- strand): ref base T (1005/567); new base A (5/103); total (1013/670)
Fisher's exact test for biased strand distribution p-value = 5.78e-37
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1700 total aligned reads displayed.

ATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTAACCTTCACGACCGCCCCACAGGACATAGTTTTCACCGCCCAATTTATGGGTTGCTTCCATCGCTGTAACAACTTGCGTTGCCGCCCAGCTGAAGACTTCAGGATCTGGGTTCGTCGCCGCACCC  >  pSIJ663_pHT315_araE_xy/8704‑8977

atatTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTaa                                                                                                                                          >  3:251818/1‑138 (MQ=255)

atatTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTaa                                                                                                                                          >  2:145675/1‑138 (MQ=255)

  atTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAg                                                                                                                                       >  5:21049/1‑139 (MQ=255)

    tGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCACCGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCg                                                                                                                                     >  4:162079/1‑139 (MQ=255)

    tGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGc                                                                                                                                      <  4:310914/138‑1 (MQ=255)

     gATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAg                                                                                                                                       >  1:90233/1‑136 (MQ=255)

      aTGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGtt                                                                                                                                   >  1:196348/1‑139 (MQ=255)

       tGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGttt                                                                                                                                  >  3:79582/1‑139 (MQ=255)

           tGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACTGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGt                                                                                                                               >  5:55402/1‑138 (MQ=255)

           tGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAg                                                                                                                                       >  1:65137/1‑130 (MQ=255)

           tGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGt                                                                                                                               >  2:4035/1‑138 (MQ=255)

           tGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGt                                                                                                                               >  7:78263/1‑138 (MQ=255)

           tGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGt                                                                                                                               >  7:245628/1‑138 (MQ=255)

           tGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGt                                                                                                                               >  1:236096/1‑138 (MQ=255)

           tGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGt                                                                                                                               >  6:206678/1‑138 (MQ=255)

           tGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGt                                                                                                                               >  4:129118/1‑138 (MQ=255)

           tGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTAGTTAACAGCGTTTCGt                                                                                                                               >  8:61751/1‑138 (MQ=255)

            gttCGGTTCTTCCGGTTTCCGTTCGAAAAGAAACGTCCCATGCACACCGATTTTATGTTTATGCACAACCACACTCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTa                                                                                                                              <  7:130826/136‑1 (MQ=255)

            gggcGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTa                                                                                                                              <  3:64773/135‑1 (MQ=255)

            ggTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTa                                                                                                                              <  8:305495/138‑1 (MQ=255)

            ggTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTa                                                                                                                              <  8:178695/138‑1 (MQ=255)

            ggTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTa                                                                                                                              <  7:31974/138‑1 (MQ=255)

            ggTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTa                                                                                                                              <  3:113691/138‑1 (MQ=255)

            ggTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTa                                                                                                                              <  4:268459/138‑1 (MQ=255)

            ggTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTa                                                                                                                              <  3:148917/138‑1 (MQ=255)

            ggTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTa                                                                                                                              <  7:23070/138‑1 (MQ=255)

            ggTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTa                                                                                                                              <  7:167799/138‑1 (MQ=255)

            ggTCGGTTCTTGCCGTTTCGGTTCGATAAGCAACCTGCCCTGGAAACCGATTTTAAGTTTATGCGCAACCCCCATCTGCAAAAAGCGGCCACGTTGTTCACGCTCCTTACGCAAGTCCGTATTTAACATCGGTTCGTaa                                                                                                                             >  2:264056/1‑139 (MQ=255)

                ggTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTAACCtt                                                                                                                         >  6:143716/1‑139 (MQ=255)

                        cGGTTTCGGTTCTATACGAAACGTGCCCTGTAAACGGTTTTTATGTTTATGCTCAACCACCATCTGCTTAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGTTATTTAACAGCTTTTCTTAACCTTCACGACCg                                                                                                                 <  1:1011/139‑1 (MQ=255)

                           tttCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTACACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTAACCTTCACGACCGccc                                                                                                              >  3:172057/1‑139 (MQ=255)

                            ttCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTACCCTTCACGCCCGcccc                                                                                                             >  1:147318/1‑139 (MQ=255)

                            ttCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTAACCTTCACGACCGcccc                                                                                                             >  3:92080/1‑139 (MQ=255)

                            ttCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTAACCTTCACGACCGcccc                                                                                                             >  3:298063/1‑139 (MQ=255)

                            ttCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTAACCTTCACGACCGccc                                                                                                              >  5:259438/1‑138 (MQ=255)

                            ttCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAAAAGCGTTTCGTAACCTTCACGCCCGcccc                                                                                                             >  5:160091/1‑139 (MQ=255)

                            ttCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGGTCACGCTCCTGCCGCAAGTCGGTATTTAACAGCGTTTCGTAACCTTCACGACCGcccc                                                                                                             >  7:185646/1‑139 (MQ=255)

                               ggTTCTATAAGCACGGGGCCCGGCAAACCTATTTTTTGTTTAGGCCCAACCACCATCTGAATACTGCGCCCCTGTTGTTCACGCGCCTGACGCAAGTCGGTATTAAACAGCGTTTCGTAACCTTCACGACCGCCCcaca                                                                                                          <  6:276432/139‑1 (MQ=255)

                               ggTGCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGGTCACGCGCCTGCCGCACGTCGTTATTTAACAGGGTTTCTTAACCTGCACGACCGCCCcaca                                                                                                          <  5:73520/139‑1 (MQ=255)

                                   cGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTAACCTTCACGACCGCCCCACAGGAc                                                                                                      <  3:309161/139‑1 (MQ=255)

                                   cGATAAGCAACGTGCCCTGGAAACCGATTTTATCTTTATGCTCAACCCCCATCTGCATAAAGCGGCCCAGTTGTTCCCGCTCCTGACCCAAGTCGGAATTTAACAGCGTTGCGTAACCTGCACCCCCGCCCCCCAGGa                                                                                                       >  6:86119/1‑138 (MQ=255)

                                     aTAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTa                                                                                                                              >  2:210345/1‑113 (MQ=255)

                                           aaCGTGCCCTGGAAACCGATTTTATGTTTATGCACAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTa                                                                                                                              <  1:164983/107‑1 (MQ=255)

                                                  ccTGCAAACAGATTTTATGTTAATCCTCAACCACCAACGCAATAACGCGGCCCAGTTGATCACGCTCCTGACCCAAGTGGGTATTTAACCGCGTTCCGTAACATACACGACCGCCCCACAGGACATAGTTTTCACCGcc                                                                                       <  6:231143/139‑1 (MQ=255)

                                                     ggAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGGATTTAACAGCGTTTCGTAACCTTCACGACCGCCCCGCAGGACATAGTTTTCACCGCCCaa                                                                                    >  8:22588/1‑139 (MQ=255)

                                                                ttatgtttatgCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTa                                                                                                                              >  2:234490/1‑86 (MQ=255)

                                                                  atgtttatgCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGAGTTTCGTAACCTTCACGACCGCCCCACAGGACATAGTTAACACCGCACAATTTATGGGCTgt                                                                        >  5:95455/1‑137 (MQ=255)

                                                                              aaCCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTAACCTTCACGACCGCCCCACAGGACATAGTTTTCACCGCCCAATTTATGGGTTGCTTCCATCGCTGt                                                            >  7:209693/1‑138 (MQ=255)

                                                                               accaccATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTAACCTTCACGACCGCCCCACAGGACATAGTTTTCACCGCCCAATTTATGGGTTGCTTCCATCGCTGTaa                                                          >  3:127420/1‑139 (MQ=255)

                                                                               accaccAACAGAATAAGGCGGCCCAGTTGTTCACGCTCCTGACGCCAGTTGTTATTAAACCGGGTTTCGTAACATCCACGACCGCCCCACAGGACATAGTTTTCACCGCCCAATTTATGGGTTGCTTCCATCGCTGTaa                                                          <  8:245628/139‑1 (MQ=255)

                                                                                   ccaTCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTAACCTTCACGACCGCCCCACAGGACATAGTTTTCACCGCCCAATTTATGGGTTGGTTCCATCGCTGTaacaac                                                      >  3:199891/1‑139 (MQ=255)

                                                                                   ccaTCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTAACCTTCACGACCGCCCCACAGGACATAGTTTTCACCGCCCAATTTATGGGTTGCTTCCATCGCTGTaacaac                                                      >  7:44113/1‑139 (MQ=255)

                                                                                    caTCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTAACCTTCACGACCGCCCCACAGGACATAGTTTTCACCGCCCAATTTATGGGTTGCTTCCATCGCTGTAACAACt                                                     >  2:16540/1‑139 (MQ=255)

                                                                                     aacTGCATAAAGCGGCCCAGTTGTTCACCCTCCTCACGCAAGTCGGTATTTAACAGCGTTTCGTAACCTTCACGACCGCCCCACACTACATAGTTTACACCGCCCAATTTATGGGTTGCTTCAATCGCTGTAACAACtt                                                    >  8:203038/3‑139 (MQ=255)

                                                                                      tCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCg                                                                                                                                     >  2:109148/1‑57 (MQ=255)

                                                                                                gcgcCCCCGTTGCACACGCCCCTCACGCACGGCGGTATAAAACCGCGTTTCGAAACCACCACGACCGCCCCACAGGACATAGTTTTCACCGCCCAATTTATGGGTTGCTTCCATCGCTGTAACAACTTGCGTTgccgcc                                         <  5:31670/139‑1 (MQ=255)

                                                                                                gCGTCCCATTTGTTCACGCCCCTGACGCAAGTCGGTTTTTAACTGGGTTTCGAACCCTTCACGACCGCCCCACAGGACATAGTTTTCACCGCCCAATTTATGGGTTGCTTCCATCGCTGTAACAACTTGCGTTgccgcc                                         <  7:61145/139‑1 (MQ=255)

                                                                                                gCGGCCCAGTTGTTCACGCGCGTGACGCAAGTCGGTATTTCACCGCGTTTCGTCCCCCCCACGACCGCCCCACAGGACATAGTTTTCACCGCCCAATTTATGGGGTGCTTCCATCGCTGTAACAACTTGCGGTgccgcc                                         <  6:109911/139‑1 (MQ=255)

                                                                                                 cGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTAACCTTCACGACCGCCCCACAGGACATAGTTTTCACCGCCCAATTTATGGGTTGCTTCCATCGCTGTAACAACTTGCGTTGCCGccc                                        >  4:110438/1‑139 (MQ=255)

                                                                                                    cccAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTACCAGCGTTTCGTAACCTTCACGACCGCCCCACAGGACATAGTTTTCACCGCCCAATTTATGGGTTGCTTCCATCGCTGTAACAACTTGCGTTGCCGCCCAGc                                     >  4:145255/1‑139 (MQ=255)

                                                                                                    cccAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGc                                                                                                                                      >  6:302658/1‑42 (MQ=255)

                                                                                                    cccAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGt                                                                                                                               >  2:67917/1‑49 (MQ=255)

                                                                                                    cccAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTa                                                                                                                              >  3:43552/1‑50 (MQ=255)

                                                                                                    cccAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTa                                                                                                                              >  1:271634/1‑50 (MQ=255)

                                                                                                    cccAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTAACCTTCACGCCCGCCCCACAGGACATAGTTTTCACCGCCCAATTTATGGGTTGCTTCCATCGCTGTAACAACTTGCGTTGCCGCCCAGc                                     >  6:44787/1‑139 (MQ=255)

                                                                                                    cccAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTAACCTTCACGATCGCCCCACAGGACATAGTTTTCACCGCCCAATTTATGGGTTGCTTCCATCGCTGTAACAACTTGCGTTGCCGCCCAGc                                     >  6:65779/1‑139 (MQ=255)

                                                                                                    cccAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTAACCTTCACGACCGCCCCACCGGACATAGTTTTCACCGCCCAATTTATGCGTTGCTTCCATCGCTGTAACAACTTGCGTTGCCGCCCAGc                                     >  4:89867/1‑139 (MQ=255)

                                                                                                    cccAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTAACCTTCACGACCGCCCCACAGGACATAGTTTTCACCGccc                                                                                      >  1:217438/1‑90 (MQ=255)

                                                                                                    cccAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTAACCTTCACGACCGCCCCACAGGACATAGTTTTCACCGCCCAATTTATggg                                                                            <  8:258787/100‑1 (MQ=255)

                                                                                                    cccAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTAACCTTCACGACCGCCCCACAGGACATAGTTTTCACCGCCCAATTTATGGGTTGCTTCCATCg                                                                >  8:43559/1‑112 (MQ=255)

                                                                                                    cccAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTAACCTTCACGACCGCCCCACAGGACATAGTTTTCACCGCCCAATTTATGGGTTGCTTCCATCGCTGTAACAACTTGCGTTgccgcc                                         >  3:278474/1‑135 (MQ=255)

                                                                                                    cccAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTAACCTTCACGACCGCCCCACAGGACATAGTTTTCACCGCCCAATTTATGGGTTGCTTCCATCGCTGTAACAACTTGCGTTGCCGCCCAGc                                     >  7:264786/1‑139 (MQ=255)

                                                                                                    cccAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTAACCTTCACGACCGCCCCACAGGACATAGTTTTCACCGCCCAATTTATGGGTTGCTTCCATCGCTGTAACAACTTGCGTTGCCGCCCAGc                                     >  7:109351/1‑139 (MQ=255)

                                                                                                    cccAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTAACCTTCACGACCGCCCCACAGGACATAGTTTTCACCGCCCAATTTATGGGTTGCTTCCATCGCTGTAACAACTTGCGTTGCCGCCCAGc                                     >  7:288557/1‑139 (MQ=255)

                                                                                                    cccAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTAACCTTCACGACCGCCCCACAGGACATAGTTTTCACCGCCCAATTTATGGGTTGCTTCCATCGCTGTAACAACTTGCGTTGCCGCCCAGc                                     >  6:161902/1‑139 (MQ=255)

                                                                                                    cccAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTAACCTTCACGACCGCCCCACAGGACATAGTTTTCACCGCCCAATTTATGGGTTGCTTCCATCGCTGTAACAACTTGCGTTGCCGCCCAGc                                     >  5:233513/1‑139 (MQ=255)

                                                                                                    cccAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTAACCTTCACGACCGCCCCACAGGACATAGTTTTCACCGCCCAATTTATGGGTTGCTTCCATCGCTGTAACAACTTGCGTTGCCGCCCAGc                                     >  4:204667/1‑139 (MQ=255)

                                                                                                    cccAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTAACCTTCACGACCGCCCCACAGGACATAGTTTTCACCGCCCAATTTATGGGTTGCTTCCATCGCTGTAACAACTTGCGTTGCCGCCCAGc                                     >  1:42423/1‑139 (MQ=255)

                                                                                                    cccAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGGTTCGTAACCTTCACGACCGCCCCACAGGACATAGTTTTCACCGCCCAATTTATGGGTTGCTTCCATCGCTGTAACAACTTGCGTTGCCGCCCAGc                                     >  5:127262/1‑139 (MQ=255)

                                                                                                    cccAGTTGTTCACGCTCCTGACGCAAGTCGGGATTTAACAGCGTTTCGGAACCTTCACGCCCGCCGCACAGGCCCTAGTTTTCACCGCCCAATTTATGGGTTGCTTCCATCGCTGTAACCACTTGCTGTGCCGCCCAGc                                     >  6:128586/1‑139 (MQ=255)

                                                                                                      aaGTTGTTCACGCTCCTGACGCAAGTCGGTATTTACCCGCGTTTCGTAACCTGCACGACCGCCCCACAGGACATAGTTTTCACCGCCCAATTTATGGGTTGCTTCCATCGCTGTAACAACTTGCGTTGCCGCCCAGCTg                                   <  7:156545/138‑1 (MQ=255)

                                                                                                        gttgttAACGCGCCTGACGCAAGTGGGTATTAACCTGCTTTTCTAAACAAACACGACCGCCCCACAGGACATAGTTTTCACCGCCCAATTTATGGGTTGCTTCCATCGCTGTAACAACTTGCGTTGCCGCCCAGCTGaa                                 <  1:12587/139‑1 (MQ=255)

                                                                                                        gttgtgCAGGCTCCTGACGCATGTCGGTATTAAACAGCGTTTCGAAACCTCCACGACCGCCCCACAGGACATAGTTTTCACCGCCCAATTTATGGGTTGCTTCCATCGCTGTAACAACTTGCGTTGCCGCCCAGCTGaa                                 <  8:282769/139‑1 (MQ=255)

                                                                                                        gttgctCACGCTCCTGACGCATGTCGGTATTAAACACCGTTCCGTAACCTCCACGACCGCCCCACAGGACATAGTTTTCACCGCCCAATTTATGGGTTGCTTCCATCGCTGTAACAACTTGCGTTGCCGCCCAGCTGaa                                 <  5:111720/139‑1 (MQ=255)

                                                                                                         ttgttCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTAACCTTCACGACCGCCCCACAGGACATAGTTTTCACCGCCCAATTTATGGGTTGCTTCCATCGCTGTAACAACTTGCGTTGCCGCCCAGCTGaa                                 <  7:94362/138‑1 (MQ=255)

                                                                                                         ttgttCACGCTCCTGACCCATGTGGGTTTTTAACAGCTTTTCTAAACCTTCACGACCGCCCCACAGGACATAGTTTTCACCGCCCAATTTATGGGTTGCTTCCATCGCTGTAACAACTTGCGTTGCCGCCCAGCTGaa                                 <  4:235614/138‑1 (MQ=255)

                                                                                                              cccGCTCCTGCCCCACGGCGGTATTAACCAGCGTTTCGTACCCTCCACGACCGCCCCACAGGACATAGTTTTCACCGCCCAATTTATGGGTTGCTTCCATCGCTGTAACAACTTGCGTTGCCGCCCAGCTGAAGACTTc                           <  3:21948/137‑1 (MQ=255)

                                                                                                              cACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTAACCTTCACGACCGCCCCACAGGACATAGTTTTCACCGCCCAATTTATGGGTTGCTTCCATCGCTGTAACAACTTGCGTTGCCGCCCAGCTGAAGACTTc                           >  2:298132/1‑139 (MQ=255)

                                                                                                              cACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTAACCTTCACGACCGCCCCACAGGACATAGTTTTCACAGCCCAATTTATGGGTTGCTTCCATCGCTGTAACAACTTGCGTTGCCGCCCAGCTGAAGACtt                            >  1:2943/1‑138 (MQ=255)

                                                                                                               aCGCGCCTGAGGCTAGTGGTTATTAAACCGGGTTTCGTAACCTCCACGACCGCCGCACAGGACATAGTTTTCACCGCCCAATTTATGGGTTGCTTCCATCGCTGTAACAACTTGCGTTGCCGCCCAGCTGAAGACTTCa                          <  4:70359/139‑1 (MQ=255)

                                                                                                                 gCTCCTGACGCTAGTCGGTATTTAACAGCGGTTCGTAACCTTCACGCCCGCCCCACAGGACATAGTTTTCACCGCCCAAGTTATGGGTTGCTTCCATCGCTGTAACAACTTGCGTTGCCGCCCAGCTGAAGACtggagg                        >  8:79290/1‑134 (MQ=255)

                                                                                                                 gCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTAACCTTCACGACCGCCCca                                                                                                            >  8:159947/1‑55 (MQ=255)

                                                                                                                   tCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTAACCTTCACGACCGCCCCACAGGACATAGTTTTCACCGCCCAATTTATGGGTTGCTTCCATCGCTGTAACAACTTGCGTTGCCGCCCAGCTGAAGACTTCAGGAt                      >  8:122220/1‑139 (MQ=255)

                                                                                                                      tGACGCAAGTCGGTATTTAACAGCGTTTCGTAACCTTCACGACCGCCCCACAGGACATAGTTTTCACCGCCCAATTTATGGGTTGCTTCCATCGCTGTAACAACTTGCGTTGCCGCCCAGCTGAAGACTTCAGGATCTg                   >  2:9415/1‑139 (MQ=255)

                                                                                                                          gCAAGTCGGTATTTAACAGCGTTTCGTAACCTTCACGACCGCCCCACAGGACATAGTTTTCACCGCCCAATTTATGGGGTGCTTCCATCGCTGTAACAACTTGCGTTGCCGCCCAGCTGAAGACTTCAGGATCTGGGtt               <  4:292301/139‑1 (MQ=255)

                                                                                                                             aGTCGGTATTTAACAGCGTTTCGTAACCTTCACGACCGCCCCACAGGACATAGTTTTCACCGCCCAATTTATGGGTTGGTTCCATCGCTGTACCAACTTGCGTTGCCGCCCAGCTGATGACGTCAGGATCTGGGTtcgt            >  5:141795/1‑139 (MQ=255)

                                                                                                                             aGTCGGTATTTAACAGCGTTTCGTAACCTTCACGACCGCCCCACAGGACATAGTTTTCACCGCCCAATTTATGGGTTGCTTCCATCGCTGTAACAACTTGCGTTGCCGCCCAGCTGAAGACTTCAGGATCTGGGTtcgt            >  4:46321/1‑139 (MQ=255)

                                                                                                                             aGTCGGTATTTAACAGCGTTTCGTAACCTTCACGACCGCCCCACAGGACATAGTTTTCACCGCCCAATTTATGGGTTGCTTCCATCGCTGTAACAACTTGCGTTGCCGCCCAGCTGAAGACTTCAGGATCTGGGTtcgt            >  2:190012/1‑139 (MQ=255)

                                                                                                                              gTCGGTATTTAACAGCGTTTCGTAACCTTCACGCCCGCCCCACAGGACATAGTTTTCACCGCCCAATTTATGGGTTGCTTCCATCGCTGTAACAACTTGCGTTGCCGCCCAGCTGAAGACTTCAGGATCTGGGTtcgtc           >  5:282390/1‑139 (MQ=255)

                                                                                                                               tctgTATTTAACAGAGTTTCGTAACCTTCCCGCCCGCCCCACAGGACATAGTTTTCACCGCCAAATTTATGGTTTGCTTCAATCGCTGTAACAACTTGCGTTGCCGCCCAGATGAATACTTCAGGCTCTGGGttcttcc          >  6:262736/4‑138 (MQ=255)

ATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTAACCTTCACGACCGCCCCACAGGACATAGTTTTCACCGCCCAATTTATGGGTTGCTTCCATCGCTGTAACAACTTGCGTTGCCGCCCAGCTGAAGACTTCAGGATCTGGGTTCGTCGCCGCACCC  >  pSIJ663_pHT315_araE_xy/8704‑8977

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`