Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 2,181,363 C→A 12.2% A121A (GCG→GCT)  U712_10920 ← Menaquinol‑cytochrome c reductase cytochrome b/c subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,181,3630CA12.2% 86.7 / 5.1 33A121A (GCG→GCT) U712_10920Menaquinol‑cytochrome c reductase cytochrome b/c subunit
Reads supporting (aligned to +/- strand):  ref base C (14/15);  new base A (2/2);  total (16/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.40e-01

TTCTTCTGCCTTGGCCCAGTCGTGAGTAGCAACTGACTGCCACGTCAAAAATACAGCAGCGGAAATCGCCAAAAGCATCATACCAACAGCAACAGGACGCTTCCACGGCCTTCTCTCTGTTCCTCTGTCAAGGAATGGCGCCAAAAGCAGGGCGCCAAAAGCGAGTCCCGGCATAATCATGGCGCCGACCACTGTAAAGCTTCCGGCGGCGTATTCATACTTCAACAGCTGGTATAAGAAAAGAAAATACCAATCCGGCAGCGGAATATAACCTGT  >  CP006881/2181225‑2181500
                                                                                                                                          |                                                                                                                                         
ttcttcTGCCTTGGCCCAGTCGTGAGTAGCAACTGACTGCCAAGTCAAAAAAAAAGAAGCGGAAATCGCCAAAAGCAACATACCAACACCAACAGGACGCTACCACGGCCTACTCTCTCTTCCACTGACAATGAATGgc                                                                                                                                           >  8:189051/1‑139 (MQ=255)
            ggCCCAGTCGTGAGTAGCATCTGTCTGCCACGTCAAAAATACAGCAGCGGAAATCGCCAAAAGCATCTTACCAACAGCAACAGGACGCTTCCACGGCCTTCTCTCTGTTCCTCTGTCAAGGAATGGCGCCAAAAGCAg                                                                                                                                <  7:189051/138‑1 (MQ=255)
            ggCCCAGTCGTGAGTAGCAACTGACTGCCACGTCAAAAATACAGCAGCGGAAATCGCCAAAAGCATCATACCAACAGCAACAGGACGCTTCCACGGCCTTCTCTCTGTTCCTCTGTCAAGGAATGGCGCCAAAAGCAg                                                                                                                                <  7:185329/138‑1 (MQ=255)
            ggCCCAGTCGTGAGTAGCAACTGACTGCCACGTCAAAAATACAGCAGCGGAAATCGCCAAAAGCATCATACCAACAGCAACAGGACGCTTCCACGGCCTTCTCTCTGTTCCTCTGTCAAGGAATGGCGCCAAAAGCAg                                                                                                                                <  5:15009/138‑1 (MQ=255)
                  gTCGTGAGTAGCAACTGACTGCCCCGTCAAAAATACAGCAGCGGAAATCGCCAAAAGCATCATACCAACACCAACAGGACGCTTCCACGGCCTTCTCGCTGTTGAACTGGAAAGGAAATGCGCCAAAACCACGGCGccc                                                                                                                         >  6:15009/1‑138 (MQ=255)
                  gTCGTGAGTAGCAACTGACTGCCACGTCAAAAATACAGCAGCGGAAATCGCCAAAAGCATCATACCAACAGCAACAGGACGCTTCCACGGCCTTCTCTCTGTTCCTCTGTCAAGGAATGGCGCCAAAAGCAGGGCGcc                                                                                                                          >  5:71035/1‑138 (MQ=255)
                             cAACTGACTGCCACGTCAAAAATACAGCAGCGGAAATCGCCAAAAGCATCATACCAACAGCAACAGGACGCTTCCACGGCCTTCTCTCTGTTCCTCTGTCAAGGAATGGCGCCAAAAGCAGGGCGCCAAAAGCGAGTcc                                                                                                              >  2:235974/1‑139 (MQ=255)
                             cAACTGACTGCCACGTCAAAAATACAGCAGCGGAAATCGCCAAAAGCATCATACCAACAGCAACAGGACGCTTCCACGGCCTTCTCTCTGTTCCTCTGTCAAGGAATGGCGCCAAAAGCAGGGCGCCAAAAGCGAGTcc                                                                                                              <  6:71035/139‑1 (MQ=255)
                                             cAAAAATACAGCAGCGGAAATCGCCAAAAGCATCATACCAACAGCAACAGGACGCTTCCACGGCCTTCTCTCTGTTCCTCTGTCAAGGAATGGCGCCAAAAGCAGGGCGCCAAAAGCGAGTCCCGGCATAATCATGgcg                                                                                              <  1:235974/139‑1 (MQ=255)
                                                        cagcGGAAATCGCCAAAAGCATCATACCAACAGCAACAGGACGCTTCCACGGCCTTCTCTCTGTTCCTCTGTCAAGGAATGGCGCCAAAAGCAGGGCGCCAACAGCGAGTCCCGGCCTAATCATGGCGCCGCCCCCTg                                                                                    >  4:256569/1‑138 (MQ=255)
                                                           cGGAAATCGCCAAAAGCATCATACCAACAGCAACAGGACGCTTCCACGGCCTTCGCTCGGTTCGTCAGGCAAGGAATGGCGCCAAAAGAAGGGCGCCAAAAGCGACTACCGGCATAATCATGGCGCCGACCACTGTa                                                                                  >  4:78982/1‑137 (MQ=255)
                                                                     cAAAAGCATCATACCAACAGCAACAGGACGCTTCCACGGCCTTCTCTCTGTTCCTCTGTCAAGGAATGGCGCCAAAAGCAGGGCGCCAAAAGCGAGTCCCGGCATAATCATGGCGCCGCCCACTGTAAAGCTTCcggcg                                                                      >  2:195629/1‑139 (MQ=255)
                                                                        aaGCATCATACCGACAGCGACCGGACGCTTCCACGGCCTTCTTTCTGTCCCTCTGTCTAGGAATGGAGCCAAAAGCAGGGCCCCAAAAGCGAGACCTGGCATAATCATGGCGCCGAc                                                                                         >  1:41862/1‑117 (MQ=255)
                                                                        aaGCATCATACCGACAGCGACCGGACGCTTCCACGGCCTTCTTTCTGTCCCTCTGTCTAGGAATGGAGCCAAAAGCAGGGCCCCAAAAGCGAGACCTGGCATAATCATGGCGCCGAc                                                                                         <  2:41862/117‑1 (MQ=255)
                                                                            atcatACCAACAGCAACAGGACGCTTCCACGGCCTTCTCTCTGTTCCTCTGTCAAGGAATGGCGCCAAAACCAGGGCGCCAAAAGCGAGTCCCGGCATAATCATGGCGCCGACCACTGTAAAGCTTCCGGCGGCGTAtt                                                               <  5:89452/139‑1 (MQ=255)
                                                                              catACCAACAGCAACAGGACGCTTCCACGGCCTTCTCTCTGTTCCTCTGTCAAGGAATGGCGCCAAAAGCAGGGCGCCAAAAGCGAGTCCCGGCATAATCATGGCGCCGACCACTGTAAAGCTTCCGGCGGCGTATTCa                                                             <  1:195629/139‑1 (MQ=255)
                                                                                                 cGCTTCCACGGCCTTCTCTCTGTTCCTCTGTCAAGGAATGGCGCCAAAAGCAGGGCGCCAAAAGCGAGTCCCGGCATAATCATGGCGCCGACCACTGTAAAGCTTCCGGCGGCGTATTCATACTTCAACAGCTGGtat                                           >  5:110516/1‑138 (MQ=255)
                                                                                                    ttCCACGGCCTTCTCTCTGTTCCTCTGTCAAGGAATGGCGCCAAAAGCAGGGCGCCAAAAGCGAGTCCCGGCATAATCATGGCGCCGACCCCTGTAAAGCTTCCGGTGGCGTATTCATACTTGAACAGCTGGttttag                                        >  8:164222/1‑133 (MQ=255)
                                                                                                      ccACGGCCTTCTCTCTGTTCCTCTGTCAAGGAATGGCGCCAAAAGCAGGGCGCCAAAAGCGAGTCCCGGCATAATCATGGCGCCGACCACTGTAAAGCTTCCGGCGGCGTATTCATACTTCAACAGCTGGTATaagaa                                      <  5:81071/138‑1 (MQ=255)
                                                                                                            ccTTCTCTCTGTTCCTCTGTCAAGGAATGGCGCCAAAAGCAGGGCGCCAAAAGCGAGTCCCGGCATAATCATGGCGCCGAc                                                                                         <  8:87914/81‑1 (MQ=255)
                                                                                                            ccTTCTCTCTGTTCCTCTGTCAAGGAATGGCGCCAAAAGCAGGGCGCCAAAAGCGAGTCCCGGCATAATCATGGCGCCGAc                                                                                         >  7:87914/1‑81 (MQ=255)
                                                                                                              ttCACTCTGTTCCTCTGTCAAGGAATGGCGCCAAAAGCAGGGCGCCAAAAGCGAGTCCCGGCATAATCATGGCGCCGACCACTGTAAAGCTTCCCGCGGCGTATTCATAATTAAACACCGTGTTTAAgaaaagaaaaa                              >  6:81071/1‑137 (MQ=255)
                                                                                                                ctctctGTTCCTATGTCAAGGAATGGCGCCAAAAGCAGGGCGCCAAAAGCGAGTCCCGGCATAATCATGGCGCCGACCACTGTAAAGCATCCGGCGGCGTATTCATACATCAACACCTGGTATAAGAAAAGAAAAAAc                            >  4:176204/1‑138 (MQ=255)
                                                                                                                      gtcccTCTGTCTAGGAATGGAGCCAAAAGCAGGGCCCCAAAAGCGAGACCTGGCATAATCATGGCGCCGACCACTGTAAAGCTTCCGGCGGCGTATTCATACTTCAACAGCTGGTATAAGAAAAGAAAATAc                            >  3:286317/4‑132 (MQ=255)
                                                                                                                      gtcccTCTGTCTAGGAATGGAGCCAAAAGCAGGGCCCCAAAAGCGAGACCTGGCATAATCATGGCGCCGACCACTGTAAAGCTTCCGGCGGCGTATTCATACTTCAACAGCTGGTATAAGAAAAGAAAATAc                            <  4:286317/129‑1 (MQ=255)
                                                                                                                      gTTCCTCTGTCAAGGAATGGCGCCAAAAGCAGGGCGCCAAAAGCGAGTCCCGGCATAATCATGGCGCCGACCACTGTa                                                                                  >  5:97654/1‑78 (MQ=255)
                                                                                                                      gTTCCTCTGTCAAGGAATGGCGCCAAAAGCAGGGCGCCAAAAGCGAGTCCCGGCATAATCATGGCGCCGACCACTGTa                                                                                  <  6:97654/78‑1 (MQ=255)
                                                                                                                      gTTCCTCTGTCAAGGAATGGCGCCAAAAGCAGGGCGCCAAAAGCGAGTCCCGGCATAATCATGGCGCCGACCACTGTAAAGCTTCCGGCGGCGTATTCATACTTCAACAGCTGGTATAAGAAAAGAAAATACCAATcct                     >  5:147294/1‑138 (MQ=255)
                                                                                                                       ttCCTATGTAAAGGAATGGCGCCAAAAGCTGGGCGCCAAAAGCGAGTCCCGGCATAATCATGGCGCCGACCACTGTAAAGCTTCCGGCGGCGTATTCATACTTCAACAGCTGGTATAAGAAAAGAAAATACCAATCCgg                    <  1:112293/139‑1 (MQ=255)
                                                                                                                          ctctGTCAAGGAATGGCGCCAAAAGCAGGGCGCCAAAAGCGAGTCCCGGCATAATCATGGCGCCGACCACTGTAAAGCTTCCGGCGGCGTATTCATACTTCAACa                                                   >  7:269033/1‑105 (MQ=255)
                                                                                                                          ctctGTCAAGGAATGGCGCCAAAAGCAGGGCGCCAAAAGCGAGTCCCGGCATAATCATGGCGCCGACCACTGTAAAGCTTCCGGCGGCGTATTCATACTTCAACa                                                   <  8:269033/105‑1 (MQ=255)
                                                                                                                             tGTCAAGGAATGGCGCCAAAAGCAGGGCGCCAAAAGCGAGTCCCGGCATAATCATGGCGCCGACCACTGTAAAGCTTCCGGTGGCGTATTCATACTTCAACAGCTGGTATAAGAAAAGAAAATACCAATCCGGCAGc                <  7:164222/137‑1 (MQ=255)
                                                                                                                             tGTCAAGGAATGGCGCCAAAAGCAGGGCGCCAAAAGCGAGTCCCGGCATAATCATGGCGCCGACCACTGTAAAGCTTCCGGCGGCGTATTCATACTTCAACAGCTGGTATAAGAAAAGAAAATACCAATCCGGCAGc                <  5:216154/137‑1 (MQ=255)
                                                                                                                                      aTGGCGCCAAAAGCAGGGCGCCAAAAGCGAGTCCCGGCATAATCATGGCGCCGACCACTGTAAAGCTTCCGGCGGCGTATTCATACTTCAACAGCTGGTATAAGAAAAGAAAATACCAATCCGGCAGCGGAATATAAcc     <  3:176204/139‑1 (MQ=255)
                                                                                                                                         gcgcCAAAAGCAGGGCGCCAAAAGCGAGTCCCGGCATAATCATGGCGCCGACCACTGTAAAGCTTCCGGCGGCGTATTCATACTTCAACAGCTGGTATAAGAAAAGAAAATACCAATCCGGCAGCGGAata          >  2:36438/1‑131 (MQ=255)
                                                                                                                                         gcgcCAAAAGCAGGGCGCCAAAAGCGAGTCCCGGCATAATCATGGCGCCGACCACTGTAAAGCTTCCGGCGGCGTATTCATACTTCAACAGCTGGTATAAGAAAAGAAAATACCAATCCGGCAGCGGAata          <  1:36438/131‑1 (MQ=255)
                                                                                                                                         gcgcCAAAAGCAGGGCGCCAAAAGCGAGTCCCGGCATAATCATGGCGCCGACCACTGTAAAGCTTCCGGCGGCGGATTCCAACTTAAACAGCGGGTAAAAGAAAAGAAAATACCAAACCCGCAGCGGAATAAAACCTGt  >  2:168500/1‑139 (MQ=255)
                                                                                                                                          |                                                                                                                                         
TTCTTCTGCCTTGGCCCAGTCGTGAGTAGCAACTGACTGCCACGTCAAAAATACAGCAGCGGAAATCGCCAAAAGCATCATACCAACAGCAACAGGACGCTTCCACGGCCTTCTCTCTGTTCCTCTGTCAAGGAATGGCGCCAAAAGCAGGGCGCCAAAAGCGAGTCCCGGCATAATCATGGCGCCGACCACTGTAAAGCTTCCGGCGGCGTATTCATACTTCAACAGCTGGTATAAGAAAAGAAAATACCAATCCGGCAGCGGAATATAACCTGT  >  CP006881/2181225‑2181500

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑